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P16015

- CAH3_MOUSE

UniProt

P16015 - CAH3_MOUSE

Protein

Carbonic anhydrase 3

Gene

Ca3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Reversible hydration of carbon dioxide.

    Catalytic activityi

    H2CO3 = CO2 + H2O.

    Cofactori

    Zinc.By similarity

    Enzyme regulationi

    Inhibited by acetazolamide.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi94 – 941Zinc; catalytic
    Metal bindingi96 – 961Zinc; catalytic
    Metal bindingi119 – 1191Zinc; catalytic
    Active sitei127 – 1271By similarity

    GO - Molecular functioni

    1. carbonate dehydratase activity Source: UniProtKB-EC
    2. nickel cation binding Source: MGI
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. one-carbon metabolic process Source: InterPro
    2. response to ethanol Source: Ensembl
    3. response to oxidative stress Source: Ensembl

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_199096. Reversible hydration of carbon dioxide.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbonic anhydrase 3 (EC:4.2.1.1)
    Alternative name(s):
    Carbonate dehydratase III
    Carbonic anhydrase III
    Short name:
    CA-III
    Gene namesi
    Name:Ca3
    Synonyms:Car3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:88270. Car3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 260259Carbonic anhydrase 3PRO_0000077427Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei182 – 1821S-glutathionyl cysteineBy similarity
    Modified residuei187 – 1871S-glutathionyl cysteineBy similarity

    Post-translational modificationi

    S-glutathionylated in hepatocytes under oxidative stress.By similarity

    Keywords - PTMi

    Acetylation, Glutathionylation

    Proteomic databases

    MaxQBiP16015.
    PaxDbiP16015.
    PRIDEiP16015.

    2D gel databases

    SWISS-2DPAGEP16015.

    PTM databases

    PhosphoSiteiP16015.

    Expressioni

    Tissue specificityi

    Expressed at lower levels in adipose tissue from animals that were either genetically obese or had experimentally induced obesity.1 Publication

    Gene expression databases

    BgeeiP16015.
    CleanExiMM_CAR3.
    GenevestigatoriP16015.

    Interactioni

    Protein-protein interaction databases

    BioGridi198484. 1 interaction.
    IntActiP16015. 4 interactions.
    MINTiMINT-1869657.
    STRINGi10090.ENSMUSP00000029076.

    Structurei

    3D structure databases

    ProteinModelPortaliP16015.
    SMRiP16015. Positions 2-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni64 – 674Involved in proton transferBy similarity
    Regioni198 – 1992Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the alpha-carbonic anhydrase family.Curated

    Phylogenomic databases

    eggNOGiCOG3338.
    GeneTreeiENSGT00750000117305.
    HOGENOMiHOG000112637.
    HOVERGENiHBG002837.
    InParanoidiP16015.
    KOiK01672.
    OMAiEAPFTHF.
    OrthoDBiEOG7WMCK7.
    PhylomeDBiP16015.
    TreeFamiTF316425.

    Family and domain databases

    Gene3Di3.10.200.10. 1 hit.
    InterProiIPR001148. Carbonic_anhydrase_a.
    IPR023561. Carbonic_anhydrase_a-class.
    IPR018338. Carbonic_anhydrase_a-class_CS.
    IPR018441. Carbonic_anhydrase_CA3.
    [Graphical view]
    PANTHERiPTHR18952. PTHR18952. 1 hit.
    PTHR18952:SF96. PTHR18952:SF96. 1 hit.
    PfamiPF00194. Carb_anhydrase. 1 hit.
    [Graphical view]
    SMARTiSM01057. Carb_anhydrase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51069. SSF51069. 1 hit.
    PROSITEiPS00162. ALPHA_CA_1. 1 hit.
    PS51144. ALPHA_CA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16015-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKEWGYASH NGPDHWHELY PIAKGDNQSP IELHTKDIKH DPSLQPWSAS    50
    YDPGSAKTIL NNGKTCRVVF DDTYDRSMLR GGPLSGPYRL RQFHLHWGSS 100
    DDHGSEHTVD GVKYAAELHL VHWNPKYNTF GEALKQPDGI AVVGIFLKIG 150
    REKGEFQILL DALDKIKTKG KEAPFTHFDP SCLFPACRDY WTYHGSFTTP 200
    PCEECIVWLL LKEPMTVSSD QMAKLRSLFS SAENEPPVPL VGNWRPPQPV 250
    KGRVVRASFK 260
    Length:260
    Mass (Da):29,366
    Last modified:January 23, 2007 - v3
    Checksum:iF4E9FE69A3C324BB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 91S → R in AAA37355. (PubMed:2496681)Curated
    Sequence conflicti86 – 861G → R in AAA37355. (PubMed:2496681)Curated
    Sequence conflicti126 – 1261K → R in AAA37355. (PubMed:2496681)Curated
    Sequence conflicti146 – 1461F → L in AAA37355. (PubMed:2496681)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27796 mRNA. Translation: AAA37355.1.
    AF294988
    , AF294983, AF294984, AF294985, AF294986, AF294987 Genomic DNA. Translation: AAG22029.1.
    BC011129 mRNA. Translation: AAH11129.1.
    CCDSiCCDS17250.1.
    PIRiA43641.
    RefSeqiNP_031632.2. NM_007606.3.
    UniGeneiMm.300.

    Genome annotation databases

    EnsembliENSMUST00000029076; ENSMUSP00000029076; ENSMUSG00000027559.
    GeneIDi12350.
    KEGGimmu:12350.
    UCSCiuc008oqs.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27796 mRNA. Translation: AAA37355.1 .
    AF294988
    , AF294983 , AF294984 , AF294985 , AF294986 , AF294987 Genomic DNA. Translation: AAG22029.1 .
    BC011129 mRNA. Translation: AAH11129.1 .
    CCDSi CCDS17250.1.
    PIRi A43641.
    RefSeqi NP_031632.2. NM_007606.3.
    UniGenei Mm.300.

    3D structure databases

    ProteinModelPortali P16015.
    SMRi P16015. Positions 2-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198484. 1 interaction.
    IntActi P16015. 4 interactions.
    MINTi MINT-1869657.
    STRINGi 10090.ENSMUSP00000029076.

    PTM databases

    PhosphoSitei P16015.

    2D gel databases

    SWISS-2DPAGE P16015.

    Proteomic databases

    MaxQBi P16015.
    PaxDbi P16015.
    PRIDEi P16015.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029076 ; ENSMUSP00000029076 ; ENSMUSG00000027559 .
    GeneIDi 12350.
    KEGGi mmu:12350.
    UCSCi uc008oqs.1. mouse.

    Organism-specific databases

    CTDi 12350.
    MGIi MGI:88270. Car3.

    Phylogenomic databases

    eggNOGi COG3338.
    GeneTreei ENSGT00750000117305.
    HOGENOMi HOG000112637.
    HOVERGENi HBG002837.
    InParanoidi P16015.
    KOi K01672.
    OMAi EAPFTHF.
    OrthoDBi EOG7WMCK7.
    PhylomeDBi P16015.
    TreeFami TF316425.

    Enzyme and pathway databases

    Reactomei REACT_199096. Reversible hydration of carbon dioxide.

    Miscellaneous databases

    ChiTaRSi CA3. mouse.
    NextBioi 281000.
    PROi P16015.
    SOURCEi Search...

    Gene expression databases

    Bgeei P16015.
    CleanExi MM_CAR3.
    Genevestigatori P16015.

    Family and domain databases

    Gene3Di 3.10.200.10. 1 hit.
    InterProi IPR001148. Carbonic_anhydrase_a.
    IPR023561. Carbonic_anhydrase_a-class.
    IPR018338. Carbonic_anhydrase_a-class_CS.
    IPR018441. Carbonic_anhydrase_CA3.
    [Graphical view ]
    PANTHERi PTHR18952. PTHR18952. 1 hit.
    PTHR18952:SF96. PTHR18952:SF96. 1 hit.
    Pfami PF00194. Carb_anhydrase. 1 hit.
    [Graphical view ]
    SMARTi SM01057. Carb_anhydrase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51069. SSF51069. 1 hit.
    PROSITEi PS00162. ALPHA_CA_1. 1 hit.
    PS51144. ALPHA_CA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse carbonic anhydrase III: nucleotide sequence and expression studies."
      Tweedie S., Edwards Y.
      Biochem. Genet. 27:17-30(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Nucleotide sequence and structure of the mouse carbonic anhydrase III gene."
      Kim G., Lee T.-H., Wynshaw-Boris A., Levine R.L.
      Gene 265:37-44(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    4. Cited for: PROTEIN SEQUENCE OF 79-96, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCAH3_MOUSE
    AccessioniPrimary (citable) accession number: P16015
    Secondary accession number(s): Q9ERN8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3