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P16009

- VG05_BPT4

UniProt

P16009 - VG05_BPT4

Protein

Tail-associated lysozyme

Gene

5

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Tail-associated lysozyme essential for localized hydrolysis of bacterial cell wall necessary for viral DNA injection. The needle-like gp5 protein punctures the outer cell membrane and then digests the peptidoglycan cell wall in the periplasmic space.1 Publication

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei184 – 1841Proton donorBy similarity
    Active sitei193 – 1931NucleophileBy similarity
    Sitei351 – 3522Cleavage

    GO - Molecular functioni

    1. lysozyme activity Source: CACAO
    2. peptidoglycan beta-N-acetylmuramidase activity Source: CACAO
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cell wall macromolecule catabolic process Source: InterPro
    2. cytolysis Source: UniProtKB-KW
    3. defense response to bacterium Source: UniProtKB-KW
    4. peptidoglycan catabolic process Source: InterPro
    5. viral entry into host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Keywords - Biological processi

    Degradation of host cell envelope components during virus entry, Degradation of host peptidoglycans during virus entry, Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Virus entry into host cell

    Protein family/group databases

    CAZyiGH24. Glycoside Hydrolase Family 24.
    TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tail-associated lysozyme (EC:3.2.1.17)
    Alternative name(s):
    Protein Gp5
    Cleaved into the following 2 chains:
    Gene namesi
    Name:5
    OrganismiEnterobacteria phage T4 (Bacteriophage T4)
    Taxonomic identifieri10665 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000009087: Genome

    Subcellular locationi

    Virion Curated

    GO - Cellular componenti

    1. virion Source: CACAO
    2. virus tail, baseplate Source: CACAO

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 575575Tail-associated lysozymePRO_0000218111Add
    BLAST
    Chaini1 – 351351Gp5*PRO_0000408360Add
    BLAST
    Chaini352 – 575224Gp5CPRO_0000408361Add
    BLAST

    Post-translational modificationi

    In the fully assembled virus, gp5 is cleaved after residue 351, but the resulting fragments, gp5* and gp5C, remain associated with the virion.1 Publication

    Expressioni

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with gp27; this interaction forms the tail lysozyme complex. The T4 tail lysozyme complex is made up of three copies of the proteolytically processed gp5 and three copies of gp27.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    27P171722EBI-1032754,EBI-1032762

    Protein-protein interaction databases

    IntActiP16009. 1 interaction.
    MINTiMINT-231912.

    Structurei

    Secondary structure

    1
    575
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi9 – 179
    Beta strandi27 – 315
    Turni32 – 343
    Beta strandi43 – 453
    Helixi49 – 513
    Beta strandi54 – 574
    Beta strandi69 – 713
    Beta strandi81 – 866
    Beta strandi94 – 996
    Beta strandi103 – 1064
    Beta strandi112 – 1154
    Beta strandi117 – 1193
    Beta strandi124 – 1296
    Helixi131 – 1344
    Helixi136 – 1405
    Helixi143 – 1497
    Beta strandi154 – 1563
    Turni163 – 1653
    Helixi176 – 1849
    Beta strandi187 – 1926
    Beta strandi194 – 1963
    Beta strandi198 – 2014
    Beta strandi204 – 2063
    Helixi214 – 22512
    Turni231 – 2344
    Helixi238 – 25720
    Helixi262 – 2687
    Helixi271 – 28414
    Helixi286 – 2905
    Helixi293 – 3008
    Helixi304 – 3118
    Helixi315 – 3184
    Turni319 – 3224
    Helixi323 – 33311
    Beta strandi334 – 3363
    Helixi337 – 3393
    Beta strandi347 – 3504
    Turni354 – 3563
    Beta strandi373 – 3775
    Beta strandi389 – 3946
    Beta strandi400 – 4045
    Beta strandi411 – 4155
    Beta strandi421 – 4244
    Beta strandi430 – 4334
    Beta strandi486 – 4927
    Beta strandi494 – 5007
    Beta strandi502 – 5087
    Beta strandi510 – 5167
    Beta strandi518 – 5247
    Beta strandi526 – 5327
    Beta strandi534 – 5407
    Beta strandi542 – 56322
    Beta strandi565 – 5684
    Beta strandi570 – 5745

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K28X-ray2.90A1-575[»]
    1PDLelectron microscopy12.00A/B/C1-575[»]
    1WTHX-ray2.80A1-575[»]
    2Z6BX-ray3.11A1-575[»]
    3A1MX-ray2.00A/B/C/D/E/F490-575[»]
    4JIVX-ray1.90A/B/C484-575[»]
    4JIWX-ray3.40A/B/C/E/F/G/I/J/K/M/N/O484-575[»]
    4JJ2X-ray1.28A/B/C483-575[»]
    4KU0X-ray1.15A/B/C484-575[»]
    DisProtiDP00284.
    ProteinModelPortaliP16009.
    SMRiP16009. Positions 6-575.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP16009.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 24 family.Curated

    Family and domain databases

    Gene3Di1.10.530.40. 1 hit.
    InterProiIPR002196. Glyco_hydro_24.
    IPR010609. Gp5_C.
    IPR009590. Gp5_OB_N_virus.
    IPR023346. Lysozyme-like_dom.
    IPR023347. Lysozyme_dom.
    IPR001165. Phage_T4_Gp5_lysozyme.
    [Graphical view]
    PfamiPF06715. Gp5_C. 3 hits.
    PF06714. Gp5_OB. 1 hit.
    PF00959. Phage_lysozyme. 1 hit.
    [Graphical view]
    PRINTSiPR00684. T4LYSOZYME.
    SUPFAMiSSF53955. SSF53955. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16009-1 [UniParc]FASTAAdd to Basket

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    MEMISNNLNW FVGVVEDRMD PLKLGRVRVR VVGLHPPQRA QGDVMGIPTE    50
    KLPWMSVIQP ITSAAMSGIG GSVTGPVEGT RVYGHFLDKW KTNGIVLGTY 100
    GGIVREKPNR LEGFSDPTGQ YPRRLGNDTN VLNQGGEVGY DSSSNVIQDS 150
    NLDTAINPDD RPLSEIPTDD NPNMSMAEML RRDEGLRLKV YWDTEGYPTI 200
    GIGHLIMKQP VRDMAQINKV LSKQVGREIT GNPGSITMEE ATTLFERDLA 250
    DMQRDIKSHS KVGPVWQAVN RSRQMALENM AFQMGVGGVA KFNTMLTAML 300
    AGDWEKAYKA GRDSLWYQQT KGRASRVTMI ILTGNLESYG VEVKTPARSL 350
    SAMAATVAKS SDPADPPIPN DSRILFKEPV SSYKGEYPYV HTMETESGHI 400
    QEFDDTPGQE RYRLVHPTGT YEEVSPSGRR TRKTVDNLYD ITNADGNFLV 450
    AGDKKTNVGG SEIYYNMDNR LHQIDGSNTI FVRGDETKTV EGNGTILVKG 500
    NVTIIVEGNA DITVKGDATT LVEGNQTNTV NGNLSWKVAG TVDWDVGGDW 550
    TEKMASMSSI SSGQYTIDGS RIDIG 575
    Length:575
    Mass (Da):63,116
    Last modified:August 1, 1990 - v2
    Checksum:i6CC2D04E05155CF5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15728 Genomic DNA. Translation: CAA33749.1.
    AF158101 Genomic DNA. Translation: AAD42482.1.
    X14845 Genomic DNA. No translation available.
    PIRiS25240. G5BPT4.
    RefSeqiNP_049757.1. NC_000866.4.

    Genome annotation databases

    GeneIDi1258817.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15728 Genomic DNA. Translation: CAA33749.1 .
    AF158101 Genomic DNA. Translation: AAD42482.1 .
    X14845 Genomic DNA. No translation available.
    PIRi S25240. G5BPT4.
    RefSeqi NP_049757.1. NC_000866.4.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K28 X-ray 2.90 A 1-575 [» ]
    1PDL electron microscopy 12.00 A/B/C 1-575 [» ]
    1WTH X-ray 2.80 A 1-575 [» ]
    2Z6B X-ray 3.11 A 1-575 [» ]
    3A1M X-ray 2.00 A/B/C/D/E/F 490-575 [» ]
    4JIV X-ray 1.90 A/B/C 484-575 [» ]
    4JIW X-ray 3.40 A/B/C/E/F/G/I/J/K/M/N/O 484-575 [» ]
    4JJ2 X-ray 1.28 A/B/C 483-575 [» ]
    4KU0 X-ray 1.15 A/B/C 484-575 [» ]
    DisProti DP00284.
    ProteinModelPortali P16009.
    SMRi P16009. Positions 6-575.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P16009. 1 interaction.
    MINTi MINT-231912.

    Protein family/group databases

    CAZyi GH24. Glycoside Hydrolase Family 24.
    TCDBi 1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1258817.

    Miscellaneous databases

    EvolutionaryTracei P16009.

    Family and domain databases

    Gene3Di 1.10.530.40. 1 hit.
    InterProi IPR002196. Glyco_hydro_24.
    IPR010609. Gp5_C.
    IPR009590. Gp5_OB_N_virus.
    IPR023346. Lysozyme-like_dom.
    IPR023347. Lysozyme_dom.
    IPR001165. Phage_T4_Gp5_lysozyme.
    [Graphical view ]
    Pfami PF06715. Gp5_C. 3 hits.
    PF06714. Gp5_OB. 1 hit.
    PF00959. Phage_lysozyme. 1 hit.
    [Graphical view ]
    PRINTSi PR00684. T4LYSOZYME.
    SUPFAMi SSF53955. SSF53955. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Functional relationships and structural determinants of two bacteriophage T4 lysozymes: a soluble (gene e) and a baseplate-associated (gene 5) protein."
      Mosig G., Lin G.W., Franklin J., Fan W.H.
      New Biol. 1:171-179(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Mosig G.
      Submitted (MAR-1990) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Sequencing, cloning and overexpression of genes of bacteriophage T4 between map positions 74.325 and 77.184."
      Koch T., Lamm N., Rueger W.
      Nucleic Acids Res. 17:4392-4392(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64.
    5. "Isolation and characterization of the bacteriophage T4 tail-associated lysozyme."
      Nakagawa H., Arisaka F., Ishii S.
      J. Virol. 54:460-466(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, CHARACTERIZATION.
    6. "Homotrimeric, beta-stranded viral adhesins and tail proteins."
      Weigele P.R., Scanlon E., King J.
      J. Bacteriol. 185:4022-4030(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INTERACTION WITH GP27, PROTEOLYTIC CLEAVAGE.
    7. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.0 ANGSTROMS).
    8. Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
    9. "Control of bacteriophage T4 tail lysozyme activity during the infection process."
      Kanamaru S., Ishiwata Y., Suzuki T., Rossmann M.G., Arisaka F.
      J. Mol. Biol. 346:1013-1020(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

    Entry informationi

    Entry nameiVG05_BPT4
    AccessioniPrimary (citable) accession number: P16009
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3