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Protein

Baseplate central spike complex protein gp5

Gene

5

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall in the periplasmic space, a necessary step for viral DNA ejection into the host cell. Involved in the tail assembly.1 Publication1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

pH dependencei

Optimum pH is 5.8.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei184Proton donorBy similarity1
Active sitei193NucleophileBy similarity1

GO - Molecular functioni

  • lysozyme activity Source: UniProtKB
  • peptidoglycan beta-N-acetylmuramidase activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Keywords - Biological processi

Degradation of host cell envelope components during virus entry, Degradation of host peptidoglycans during virus entry, Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Viral tail assembly, Virus entry into host cell, Virus exit from host cell

Protein family/group databases

CAZyiGH24. Glycoside Hydrolase Family 24.
TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Baseplate central spike complex protein gp51 Publication
Alternative name(s):
Peptidoglycan hydrolase gp5 (EC:3.2.1.171 Publication)
Protein Gp5
Cleaved into the following 2 chains:
Gene namesi
Name:5
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • virion Source: CACAO
  • virus tail, baseplate Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Viral baseplate protein, Viral tail protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002181111 – 575Baseplate central spike complex protein gp5Add BLAST575
ChainiPRO_00004083601 – 351Gp5*1 PublicationAdd BLAST351
ChainiPRO_0000408361352 – 575Gp5C1 PublicationAdd BLAST224

Post-translational modificationi

In the fully assembled virus, gp5 is cleaved after residue 351, but the resulting fragments, gp5* and gp5C, remain associated with the virion.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei351 – 352Cleavage2

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer (PubMed:12837775, PubMed:19896486, PubMed:11823865). Heteromultimer with gp27 trimer and gp5.4 monomer; this interaction forms the central spike complex that creates an extension of the tail tube (PubMed:12837775, PubMed:27193680). The central spike complex is made up of three copies of the gp27-gp5*-gp5C complex and one copy of gp5.4 (PubMed:11823865, PubMed:27193680). Part of the baseplate macromolecular complex which consists of gp5, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube) (PubMed:27193680).1 Publication4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
27P171722EBI-1032754,EBI-1032762

Protein-protein interaction databases

IntActiP16009. 1 interactor.
MINTiMINT-231912.

Structurei

Secondary structure

1575
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 17Combined sources9
Beta strandi27 – 31Combined sources5
Turni32 – 34Combined sources3
Beta strandi43 – 45Combined sources3
Helixi49 – 51Combined sources3
Beta strandi54 – 57Combined sources4
Beta strandi69 – 71Combined sources3
Beta strandi81 – 86Combined sources6
Beta strandi94 – 99Combined sources6
Beta strandi103 – 106Combined sources4
Beta strandi112 – 115Combined sources4
Beta strandi117 – 119Combined sources3
Beta strandi124 – 129Combined sources6
Helixi131 – 134Combined sources4
Helixi136 – 140Combined sources5
Helixi143 – 149Combined sources7
Beta strandi154 – 156Combined sources3
Turni163 – 165Combined sources3
Helixi176 – 184Combined sources9
Beta strandi187 – 192Combined sources6
Beta strandi194 – 196Combined sources3
Beta strandi198 – 201Combined sources4
Beta strandi204 – 206Combined sources3
Helixi214 – 225Combined sources12
Turni231 – 234Combined sources4
Helixi238 – 257Combined sources20
Helixi262 – 268Combined sources7
Helixi271 – 284Combined sources14
Helixi286 – 290Combined sources5
Helixi293 – 300Combined sources8
Helixi304 – 311Combined sources8
Helixi315 – 318Combined sources4
Turni319 – 322Combined sources4
Helixi323 – 333Combined sources11
Beta strandi334 – 336Combined sources3
Helixi337 – 339Combined sources3
Beta strandi347 – 350Combined sources4
Turni354 – 356Combined sources3
Beta strandi373 – 377Combined sources5
Beta strandi389 – 394Combined sources6
Beta strandi400 – 404Combined sources5
Beta strandi411 – 415Combined sources5
Beta strandi421 – 424Combined sources4
Beta strandi430 – 433Combined sources4
Beta strandi486 – 492Combined sources7
Beta strandi494 – 500Combined sources7
Beta strandi502 – 508Combined sources7
Beta strandi510 – 516Combined sources7
Beta strandi518 – 524Combined sources7
Beta strandi526 – 532Combined sources7
Beta strandi534 – 540Combined sources7
Beta strandi542 – 563Combined sources22
Beta strandi565 – 568Combined sources4
Beta strandi570 – 574Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K28X-ray2.90A1-575[»]
1PDLelectron microscopy12.00A/B/C1-575[»]
1WTHX-ray2.80A1-575[»]
2Z6BX-ray3.11A1-575[»]
3A1MX-ray2.00A/B/C/D/E/F490-575[»]
4JIVX-ray1.90A/B/C484-575[»]
4JIWX-ray3.40A/B/C/E/F/G/I/J/K/M/N/O484-575[»]
4JJ2X-ray1.28A/B/C483-575[»]
4KU0X-ray1.15A/B/C484-575[»]
4OSDX-ray1.96A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R484-575[»]
5IV5electron microscopy4.11YA/YB/YC1-575[»]
DisProtiDP00284.
ProteinModelPortaliP16009.
SMRiP16009.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16009.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.Curated

Family and domain databases

Gene3Di1.10.530.40. 1 hit.
InterProiIPR002196. Glyco_hydro_24.
IPR010609. Gp5_C.
IPR009590. Gp5_OB_N.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
IPR001165. T4-type_lysozyme.
[Graphical view]
PfamiPF06715. Gp5_C. 3 hits.
PF06714. Gp5_OB. 1 hit.
PF00959. Phage_lysozyme. 1 hit.
[Graphical view]
PRINTSiPR00684. T4LYSOZYME.
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMISNNLNW FVGVVEDRMD PLKLGRVRVR VVGLHPPQRA QGDVMGIPTE
60 70 80 90 100
KLPWMSVIQP ITSAAMSGIG GSVTGPVEGT RVYGHFLDKW KTNGIVLGTY
110 120 130 140 150
GGIVREKPNR LEGFSDPTGQ YPRRLGNDTN VLNQGGEVGY DSSSNVIQDS
160 170 180 190 200
NLDTAINPDD RPLSEIPTDD NPNMSMAEML RRDEGLRLKV YWDTEGYPTI
210 220 230 240 250
GIGHLIMKQP VRDMAQINKV LSKQVGREIT GNPGSITMEE ATTLFERDLA
260 270 280 290 300
DMQRDIKSHS KVGPVWQAVN RSRQMALENM AFQMGVGGVA KFNTMLTAML
310 320 330 340 350
AGDWEKAYKA GRDSLWYQQT KGRASRVTMI ILTGNLESYG VEVKTPARSL
360 370 380 390 400
SAMAATVAKS SDPADPPIPN DSRILFKEPV SSYKGEYPYV HTMETESGHI
410 420 430 440 450
QEFDDTPGQE RYRLVHPTGT YEEVSPSGRR TRKTVDNLYD ITNADGNFLV
460 470 480 490 500
AGDKKTNVGG SEIYYNMDNR LHQIDGSNTI FVRGDETKTV EGNGTILVKG
510 520 530 540 550
NVTIIVEGNA DITVKGDATT LVEGNQTNTV NGNLSWKVAG TVDWDVGGDW
560 570
TEKMASMSSI SSGQYTIDGS RIDIG
Length:575
Mass (Da):63,116
Last modified:August 1, 1990 - v2
Checksum:i6CC2D04E05155CF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15728 Genomic DNA. Translation: CAA33749.1.
AF158101 Genomic DNA. Translation: AAD42482.1.
X14845 Genomic DNA. No translation available.
PIRiS25240. G5BPT4.
RefSeqiNP_049757.1. NC_000866.4.

Genome annotation databases

GeneIDi1258817.
KEGGivg:1258817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15728 Genomic DNA. Translation: CAA33749.1.
AF158101 Genomic DNA. Translation: AAD42482.1.
X14845 Genomic DNA. No translation available.
PIRiS25240. G5BPT4.
RefSeqiNP_049757.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K28X-ray2.90A1-575[»]
1PDLelectron microscopy12.00A/B/C1-575[»]
1WTHX-ray2.80A1-575[»]
2Z6BX-ray3.11A1-575[»]
3A1MX-ray2.00A/B/C/D/E/F490-575[»]
4JIVX-ray1.90A/B/C484-575[»]
4JIWX-ray3.40A/B/C/E/F/G/I/J/K/M/N/O484-575[»]
4JJ2X-ray1.28A/B/C483-575[»]
4KU0X-ray1.15A/B/C484-575[»]
4OSDX-ray1.96A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R484-575[»]
5IV5electron microscopy4.11YA/YB/YC1-575[»]
DisProtiDP00284.
ProteinModelPortaliP16009.
SMRiP16009.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP16009. 1 interactor.
MINTiMINT-231912.

Protein family/group databases

CAZyiGH24. Glycoside Hydrolase Family 24.
TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258817.
KEGGivg:1258817.

Miscellaneous databases

EvolutionaryTraceiP16009.

Family and domain databases

Gene3Di1.10.530.40. 1 hit.
InterProiIPR002196. Glyco_hydro_24.
IPR010609. Gp5_C.
IPR009590. Gp5_OB_N.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
IPR001165. T4-type_lysozyme.
[Graphical view]
PfamiPF06715. Gp5_C. 3 hits.
PF06714. Gp5_OB. 1 hit.
PF00959. Phage_lysozyme. 1 hit.
[Graphical view]
PRINTSiPR00684. T4LYSOZYME.
SUPFAMiSSF53955. SSF53955. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBP5_BPT4
AccessioniPrimary (citable) accession number: P16009
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: August 1, 1990
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.