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P16006

- DCTD_BPT4

UniProt

P16006 - DCTD_BPT4

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Protein

Deoxycytidylate deaminase

Gene
CD
Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Supplies the nucleotide substrate for thymidylate synthetase.

Catalytic activityi

dCMP + H2O = dUMP + NH3.

Cofactori

Binds 2 zinc ions per subunit.

Enzyme regulationi

Allosteric enzyme whose activity is greatly influenced by the end products of its metabolic pathway, dCTP and dTTP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi19 – 191Zinc 1; structural
Metal bindingi49 – 491Zinc 1; structural
Metal bindingi94 – 941Zinc 1; structural
Metal bindingi102 – 1021Zinc 2; structural
Metal bindingi104 – 1041Zinc 2; catalytic
Active sitei106 – 1061Proton donor By similarity
Metal bindingi132 – 1321Zinc 2; catalytic
Metal bindingi135 – 1351Zinc 2; catalytic
Binding sitei153 – 1531Substrate

GO - Molecular functioni

  1. dCMP deaminase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. nucleotide biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxycytidylate deaminase (EC:3.5.4.12)
Alternative name(s):
dCMP deaminase
Short name:
dCD
Gene namesi
Name:CD
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 193193Deoxycytidylate deaminasePRO_0000171699Add
BLAST

Interactioni

Subunit structurei

Homohexamer.

Structurei

Secondary structure

1
193
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412
Beta strandi20 – 223
Beta strandi25 – 306
Beta strandi33 – 397
Helixi49 – 568
Beta strandi59 – 613
Beta strandi84 – 863
Helixi88 – 903
Helixi91 – 10111
Helixi105 – 11612
Beta strandi124 – 1296
Helixi133 – 1419
Beta strandi146 – 1516
Turni158 – 1614
Helixi162 – 1665
Beta strandi170 – 1734
Helixi176 – 1783
Helixi184 – 1863

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VQ2X-ray2.20A1-193[»]
DisProtiDP00583.
ProteinModelPortaliP16006.
SMRiP16006. Positions 1-193.

Miscellaneous databases

EvolutionaryTraceiP16006.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR016473. dCMP_deaminase.
IPR015517. dCMP_deaminase-rel.
[Graphical view]
PANTHERiPTHR11086. PTHR11086. 1 hit.
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
PIRSFiPIRSF006019. dCMP_deaminase. 1 hit.
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16006-1 [UniParc]FASTAAdd to Basket

« Hide

MKASTVLQIA YLVSQESKCC SWKVGAVIEK NGRIISTGYN GSPAGGVNCC    50
DYAAEQGWLL NKPKHAIIQG HKPECVSFGS TDRFVLAKEH RSAHSEWSSK 100
NEIHAELNAI LFAARNGSSI EGATMYVTLS PCPDCAKAIA QSGIKKLVYC 150
ETYDKNKPGW DDILRNAGIE VFNVPKKNLN KLNWENINEF CGE 193
Length:193
Mass (Da):21,198
Last modified:April 1, 1990 - v1
Checksum:iF720EF1B4A2E8CE6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05172 Genomic DNA. Translation: AAA32489.1.
AF158101 Genomic DNA. Translation: AAD42546.1.
PIRiJN0081. DUBPT4.
RefSeqiNP_049828.1. NC_000866.4.

Genome annotation databases

GeneIDi1258669.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05172 Genomic DNA. Translation: AAA32489.1 .
AF158101 Genomic DNA. Translation: AAD42546.1 .
PIRi JN0081. DUBPT4.
RefSeqi NP_049828.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VQ2 X-ray 2.20 A 1-193 [» ]
DisProti DP00583.
ProteinModelPortali P16006.
SMRi P16006. Positions 1-193.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1258669.

Miscellaneous databases

EvolutionaryTracei P16006.

Family and domain databases

InterProi IPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR016473. dCMP_deaminase.
IPR015517. dCMP_deaminase-rel.
[Graphical view ]
PANTHERi PTHR11086. PTHR11086. 1 hit.
Pfami PF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF006019. dCMP_deaminase. 1 hit.
SUPFAMi SSF53927. SSF53927. 1 hit.
PROSITEi PS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequence analysis, and expression of the bacteriophage T4 cd gene."
    Maley G.F., Duceman B.W., Wang A.-M., Martinez J., Maley F.
    J. Biol. Chem. 265:47-51(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "T4-phage deoxycytidylate deaminase is a metalloprotein containing two zinc atoms per subunit."
    Moore J.T., Silversmith R.E., Maley G.F., Maley F.
    J. Biol. Chem. 268:2288-2291(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: ZINC-BINDING.
  4. "Three-dimensional structure of the R115E mutant of T4-bacteriophage 2'-deoxycytidylate deaminase."
    Almog R., Maley F., Maley G.F., Maccoll R., Van Roey P.
    Biochemistry 43:13715-13723(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF MUTANT GLU-115 IN COMPLEX WITH SUBSTRATE ANALOG AND ZINC IONS.

Entry informationi

Entry nameiDCTD_BPT4
AccessioniPrimary (citable) accession number: P16006
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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