P15992 (HSP26_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heat shock protein 26

Alternative name(s):
26 kDa heat shock protein

Gene names

Name:	HSP26
Ordered Locus Names:	YBR072W
ORF Names:	YBR0714

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not known. One of the major polypeptides produced on heat shock.
Subunit structure	Present in large complexes.
Developmental stage	Expressed during the entry into stationary phase resulting from glucose limitation.
Induction	By heat shock, and under other conditions of stress, such as increased salt concentration and starvation.
Miscellaneous	Present with 19300 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the small heat shock protein (HSP20) family.

Ontologies

Keywords
Biological process	Stress response
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from direct assay. Source: SGD response to stress Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	mRNA binding Inferred from direct assay. Source: SGD unfolded protein binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 214	213	Heat shock protein 26		PRO_0000126004
Amino acid modifications
	Modified residue	42	1	Phosphothreonine Ref.7 Ref.9 Ref.10
	Modified residue	90	1	Phosphoserine Ref.10
	Modified residue	163	1	Phosphothreonine Ref.10
	Modified residue	208	1	Phosphoserine Ref.7 Ref.10
	Modified residue	211	1	Phosphoserine Ref.7 Ref.10
Experimental info
	Sequence conflict	32	1	G → A in AAA79010. Ref.2
	Sequence conflict	207	1	S → C in AAA79010. Ref.2
	Sequence conflict	212	1	W → G in AAT92994. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P15992 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0CB8B6BD22C5308F	FASTA	214	23,880
	10 20 30 40 50 60 MSFNSPFFDF FDNINNEVDA FNRLLGEGGL RGYAPRRQLA NTPAKDSTGK EVARPNNYAG 70 80 90 100 110 120 ALYDPRDETL DDWFDNDLSL FPSGFGFPRS VAVPVDILDH DNNYELKVVV PGVKSKKDID 130 140 150 160 170 180 IEYHQNKNQI LVSGEIPSTL NEESKDKVKV KESSSGKFKR VITLPDYPGV DADNIKADYA 190 200 210 NGVLTLTVPK LKPQKDGKNH VKKIEVSSQE SWGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"hsp26 of Saccharomyces cerevisiae is related to the superfamily of small heat shock proteins but is without a demonstrable function." Susek R.E., Lindquist S.L. Mol. Cell. Biol. 9:5265-5271(1989) [PubMed: 2689876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure and expression of a yeast gene encoding the small heat-shock protein Hsp26." Bossier P., Fitch I.T., Boucherie H., Tuite M.F. Gene 78:323-330(1989) [PubMed: 2673926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CLEAVAGE OF INITIATOR METHIONINE.
[3]	"Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II." van der Aart Q.J.M., Barthe C., Doignon F., Aigle M., Crouzet M., Steensma H.Y. Yeast 10:959-964(1994) [PubMed: 7985423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-42; SER-208 AND SER-211, MASS SPECTROMETRY. Strain: 2124.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-42, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-42; SER-90; THR-163; SER-208 AND SER-211, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M23871 Genomic DNA. Translation: AAA66914.1. M26942 Genomic DNA. Translation: AAA79010.1. X76294 Genomic DNA. Translation: CAA53929.1. Z35941 Genomic DNA. Translation: CAA85016.1. AY692975 Genomic DNA. Translation: AAT92994.1. BK006936 Genomic DNA. Translation: DAA07191.1.
PIR	S45465.
RefSeq	NP_009628.1. NM_001178420.1.
3D structure databases
ProteinModelPortal	P15992.
SMR	P15992. Positions 61-208.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3988N.
IntAct	P15992. 35 interactions.
MINT	MINT-536537.
STRING	P15992.
Proteomic databases
PeptideAtlas	P15992.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR072W; YBR072W; YBR072W.
GeneID	852364.
KEGG	sce:YBR072W.
NMPDR	fig|4932.3.peg.325.
Organism-specific databases
SGD	S000000276. HSP26.
Phylogenomic databases
eggNOG	fuNOG11705.
GeneTree	EFGT00070000008793.
HOGENOM	HBG204144.
OMA	KLEPREN.
OrthoDB	EOG490BK9.
Gene expression databases
ArrayExpress	P15992.
Genevestigator	P15992.
GermOnline	YBR072W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002068. Hsp20. IPR008978. HSP20-like_chaperone. [Graphical view]
KO	K13993.
Pfam	PF00011. HSP20. 1 hit. [Graphical view]
SUPFAM	SSF49764. HSP20_chap. 1 hit.
PROSITE	PS01031. HSP20. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971137.

Entry information

Entry name

HSP26_YEAST

Accession

Primary (citable) accession number: P15992
Secondary accession number(s): D6VQ71, Q6B1V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program