P15977 (MALQ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-alpha-glucanotransferase
EC=2.4.1.25

Alternative name(s):
Amylomaltase
Disproportionating enzyme
Short name=D-enzyme

Gene names

Name:	malQ
Synonyms:	malA
Ordered Locus Names:	b3416, JW3379

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	694 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the disproportionating enzyme family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-glucanotransferase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 694	694	4-alpha-glucanotransferase		PRO_0000170125
Experimental info
	Sequence conflict	22	1	A → P in AAA24106. Ref.1
	Sequence conflict	199 – 200	2	SP → TA in AAA24106. Ref.1
	Sequence conflict	380 – 381	2	AE → GT in AAA24106. Ref.1
	Sequence conflict	462	1	G → R in AAA24106. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P15977 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 73FFB3F8E5619311	FASTA	694	78,503
	10 20 30 40 50 60 MESKRLDNAA LAAGISPNYI NAHGKPQSIS AETKRRLLDA MHQRTATKVA VTPVPNVMVY 70 80 90 100 110 120 TSGKKMPMVV EGSGEYSWLL TTEEGTQYKG HVTGGKAFNL PTKLPEGYHT LTLTQDDQRA 130 140 150 160 170 180 HCRVIVAPKR CYEPQALLNK QKLWGACVQL YTLRSEKNWG IGDFGDLKAM LVDVAKRGGS 190 200 210 220 230 240 FIGLNPIHAL YPANPESASP YSPSSRRWLN VIYIDVNAVE DFHLSEEAQA WWQLPTTQQT 250 260 270 280 290 300 LQQARDADWV DYSTVTALKM TALRMAWKGF AQRDDEQMAA FRQFVAEQGD SLFWQAAFDA 310 320 330 340 350 360 LHAQQVKEDE MRWGWPAWPE MYQNVDSPEV RQFCEEHRDD VDFYLWLQWL AYSQFAACWE 370 380 390 400 410 420 ISQGYEMPIG LYRDLAVGVA EGGAETWCDR ELYCLKASVG APPDILGPLG QNWGLPPMDP 430 440 450 460 470 480 HIITARAYEP FIELLRANMQ NCGALRIDHV MSMLRLWWIP YGETADQGAY VHYPVDDLLS 490 500 510 520 530 540 ILALESKRHR CMVIGEDLGT VPVEIVGKLR SSGVYSYKVL YFENDHEKTF RAPKAYPEQS 550 560 570 580 590 600 MAVAATHDLP TLRGYWECGD LTLGKTLGLY PDEVVLRGLY QDRELAKQGL LDALHKYGCL 610 620 630 640 650 660 PKRAGHKASL MSMTPTLNRG LQRYIADSNS ALLGLQPEDW LDMAEPVNIP GTSYQYKNWR 670 680 690 RKLSATLESM FADDGVNKLL KDLDRRRRAA AKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of malQ, the structural gene for the Escherichia coli enzyme amylomaltase." Pugsley A.P., Dubreuil C. Mol. Microbiol. 2:473-479(1988) [PubMed: 2845225] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M32793 Genomic DNA. Translation: AAA24106.1. U18997 Genomic DNA. Translation: AAA58214.1. U00096 Genomic DNA. Translation: AAC76441.1. AP009048 Genomic DNA. Translation: BAE77875.1.
PIR	C65137.
RefSeq	NP_417875.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P15977.
SMR	P15977. Positions 141-662.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10147N.
IntAct	P15977. 6 interactions.
MINT	MINT-1275716.
Protein family/group databases
CAZy	GH77. Glycoside Hydrolase Family 77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002971; EBESCP00000002971; EBESCG00000002432. EBESCT00000002972; EBESCP00000002972; EBESCG00000002432. EBESCT00000017170; EBESCP00000016461; EBESCG00000016229.
GeneID	947923.
GenomeReviews	Gene locus JW3379 in contig AP009048_GR. Gene locus b3416 in contig U00096_GR.
KEGG	ecj:JW3379. eco:b3416.
PATRIC	32122270. VBIEscCol129921_3512.
Organism-specific databases
EchoBASE	EB0556.
EcoGene	EG10561. malQ.
Phylogenomic databases
eggNOG	COG1640.
GeneTree	EBGT00050000009129.
HOGENOM	HBG686276.
OMA	DPFAPQG.
PhylomeDB	P15977.
ProtClustDB	PRK11052.
Enzyme and pathway databases
BioCyc	EcoCyc:AMYLOMALT-MONOMER. MetaCyc:AMYLOMALT-MONOMER.
Gene expression databases
Genevestigator	P15977.
Family and domain databases
InterPro	IPR003385. Glyco_hydro_77. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
KO	K00705.
Pfam	PF02446. Glyco_hydro_77. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR00217. MalQ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MALQ_ECOLI

Accession

Primary (citable) accession number: P15977
Secondary accession number(s): Q2M781

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program