P15969 (TOG1A_AGEAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Omega-agatoxin-1A Alternative name(s): Omega-agatoxin IA Short name=Omega-Aga-IA Cleaved into the following 2 chains: Omega-agatoxin-1A major chain Omega-agatoxin-1A minor chain
Organism	Agelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Taxonomic identifier	6908 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Araneae › Araneomorphae › Entelegynae › RTA clade › Agelenidae › Agelenopsis

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Omega-agatoxin are antagonist of voltage-gated calcium channels. They block insect neuromuscular transmission presynaptically. This toxin is a blocker of L-type calcium channels (Cav/CACNA1). Ref.3
Subunit structure	Heterodimer of two subunits, a major chain and a minor chain, linked by a disulfide bond.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Proteolytically processed to yield the major and the minor chains.
Sequence similarities	Belongs to the omega-agatoxin superfamily. Type I omega-agatoxin family.
Mass spectrometry	Molecular mass is 7791.4 Da from positions 37 - 102 and 110 - 112. Determined by PD. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Calcium channel inhibitor Ion channel impairing toxin Neurotoxin Presynaptic neurotoxin Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	other organism presynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 36	17			PRO_0000035486
	Chain	37 – 102	66	Omega-agatoxin-1A major chain		PRO_0000035487
	Propeptide	103 – 109	7	Glu-rich		PRO_0000035488
	Chain	110 – 112	3	Omega-agatoxin-1A minor chain		PRO_0000035489
Experimental info
	Sequence conflict	101	1	D → N AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P15969 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: ACC0ED776E49DF23	FASTA	112	12,808
	10 20 30 40 50 60 MMKFVVFLAC LFVAAHSFAV EGEEEYFEAE VPELERAKAL PPGSVCDGNE SDCKCYGKWH 70 80 90 100 110 KCRCPWKWHF TGEGPCTCEK GMKHTCITKL HCPNKAEWGL DWRSEESERS PC

References

[1]	"Heterodimeric structure of the spider toxin omega-agatoxin IA revealed by precursor analysis and mass spectrometry." Santos A.D., Imperial J.S., Chaudhary T., Beavis R.C., Chait B.T., Hunsperger J.P., Olivera B.M., Adams M.E., Hillyard D.R. J. Biol. Chem. 267:20701-20705(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Venom.
[2]	"Omega-agatoxins: novel calcium channel antagonists of two subtypes from funnel web spider (Agelenopsis aperta) venom." Adams M.E., Bindokas V.P., Hasegawa L., Venema V.J. J. Biol. Chem. 265:861-867(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 37-102, MASS SPECTROMETRY. Tissue: Venom.
[3]	"Differential antagonism of transmitter release by subtypes of omega-agatoxins." Bindokas V.P., Venema V.J., Adams M.E. J. Neurophysiol. 66:590-601(1991) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95540 mRNA. No translation available.
PIR	A45069.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ArachnoServer	AS000175. omega-agatoxin-Aa1a.
Family and domain databases
InterPro	IPR013605. Toxin_34. [Graphical view]
Pfam	PF08396. Toxin_34. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOG1A_AGEAP

Accession

Primary (citable) accession number: P15969

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program