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P15969

- TOG1A_AGEAP

UniProt

P15969 - TOG1A_AGEAP

Protein

Omega-agatoxin-1A

Gene
N/A
Organism
Agelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Omega-agatoxin are antagonist of voltage-gated calcium channels. They block insect neuromuscular transmission presynaptically. This toxin is a blocker of L-type calcium channels (Cav/CACNA1).1 Publication

    Keywords - Molecular functioni

    Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Presynaptic neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Omega-agatoxin-1A
    Alternative name(s):
    Omega-agatoxin IA
    Short name:
    Omega-Aga-IA
    Cleaved into the following 2 chains:
    OrganismiAgelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
    Taxonomic identifieri6908 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAgelenidaeAgelenopsis

    Organism-specific databases

    ArachnoServeriAS000175. omega-agatoxin-Aa1a.

    Subcellular locationi

    GO - Cellular componenti

    1. other organism presynaptic membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Propeptidei20 – 36171 PublicationPRO_0000035486Add
    BLAST
    Chaini37 – 10266Omega-agatoxin-1A major chainPRO_0000035487Add
    BLAST
    Propeptidei103 – 1097Glu-richPRO_0000035488
    Chaini110 – 1123Omega-agatoxin-1A minor chainPRO_0000035489

    Post-translational modificationi

    Proteolytically processed to yield the major and the minor chains.

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Interactioni

    Subunit structurei

    Heterodimer of two subunits, a major chain and a minor chain, linked by a disulfide bond.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR013605. Toxin_34.
    [Graphical view]
    PfamiPF08396. Toxin_34. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15969-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMKFVVFLAC LFVAAHSFAV EGEEEYFEAE VPELERAKAL PPGSVCDGNE    50
    SDCKCYGKWH KCRCPWKWHF TGEGPCTCEK GMKHTCITKL HCPNKAEWGL 100
    DWRSEESERS PC 112
    Length:112
    Mass (Da):12,808
    Last modified:November 1, 1997 - v2
    Checksum:iACC0ED776E49DF23
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti101 – 1011D → N AA sequence (PubMed:2295621)Curated

    Mass spectrometryi

    Molecular mass is 7791.4 Da from positions 37 - 102 and 110 - 112. Determined by PD. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95540 mRNA. No translation available.
    PIRiA45069.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95540 mRNA. No translation available.
    PIRi A45069.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    ArachnoServeri AS000175. omega-agatoxin-Aa1a.

    Family and domain databases

    InterProi IPR013605. Toxin_34.
    [Graphical view ]
    Pfami PF08396. Toxin_34. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Heterodimeric structure of the spider toxin omega-agatoxin IA revealed by precursor analysis and mass spectrometry."
      Santos A.D., Imperial J.S., Chaudhary T., Beavis R.C., Chait B.T., Hunsperger J.P., Olivera B.M., Adams M.E., Hillyard D.R.
      J. Biol. Chem. 267:20701-20705(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY.
      Tissue: Venom.
    2. "Omega-agatoxins: novel calcium channel antagonists of two subtypes from funnel web spider (Agelenopsis aperta) venom."
      Adams M.E., Bindokas V.P., Hasegawa L., Venema V.J.
      J. Biol. Chem. 265:861-867(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 37-102, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Venom.
    3. "Differential antagonism of transmitter release by subtypes of omega-agatoxins."
      Bindokas V.P., Venema V.J., Adams M.E.
      J. Neurophysiol. 66:590-601(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiTOG1A_AGEAP
    AccessioniPrimary (citable) accession number: P15969
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3