Reviewed, UniProtKB/Swiss-Prot P15964 (GST26_SCHMA)

Names and origin

Protein names	Recommended name:     Glutathione S-transferase class-mu 26 kDa isozyme       Short name=GST 26     EC=2.5.1.18 Alternative name(s):     Sm26/1 antigen     SmGST
Organism	Schistosoma mansoni (Blood fluke)
Taxonomic identifier	6183 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Platyhelminthes › Trematoda › Digenea › Strigeidida › Schistosomatoidea › Schistosomatidae › Schistosoma

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Tissue specificity	Tegument and in subtegumentary parenchymal cells. GST 26 may be actively excreted by adult worms.
Miscellaneous	There are at least three isoenzymes of GST in S.mansoni.
Sequence similarities	Belongs to the GST superfamily. Mu family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Gene Ontology (GO)
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Glutathione S-transferase class-mu 26 kDa isozyme		PRO_0000185806
Regions
	Domain	1 – 83	83	GST N-terminal
	Domain	85 – 203	119	GST C-terminal
Sites
	Active site	7	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15964-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 061A6548A842D6E8	FASTA	218	25,401
	10 20 30 40 50 60 MAPKFGYWKV KGLVQPTRLL LEHLEETYEE RAYDRNEIDA WSNDKFKLGL EFPNLPYYID 70 80 90 100 110 120 GDFKLTQSMA IIRYIADKHN MLGACPKERA EISMLEGAVL DIRMGVLRIA YNKEYETLKV 130 140 150 160 170 180 DFLNKLPGRL KMFEDRLSNK TYLNGNCVTH PDFMLYDALD VVLYMDSQCL NEFPKLVSFK 190 200 210 KCIEDLPQIK NYLNSSRYIK WPLQGWDATF GGGDTPPK

References

[1]	"Molecular cloning and tissue distribution of a 26-kilodalton Schistosoma mansoni glutathione S-transferase." Trottein F., Kieny M.P., Verwaerde C., Torpier G., Pierce R.J., Balloul J.-M., Schmitt D., Lecocq J.-P., Capron A. Mol. Biochem. Parasitol. 41:35-44(1990) [PubMed: 2385266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Puerto Rican.
[2]	"Comparison of the cloned genes of the 26- and 28-kilodalton glutathione S-transferases of Schistosoma japonicum and Schistosoma mansoni." Henkle K.J., Davern K.M., Wright M.D., Ramos A.J., Mitchell G.F. Mol. Biochem. Parasitol. 40:23-34(1990) [PubMed: 1693415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-218. Strain: Puerto Rican.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31106 mRNA. Translation: AAA29888.1. M26913 mRNA. Translation: AAA29889.1.
PIR	A45523.
3D structure databases
SMR	P15964. Positions 2-218.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.18. 1460.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GST26_SCHMA

Accession

Primary (citable) accession number: P15964

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	November 24, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)