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Protein

RNA-directed RNA polymerase

Gene

ORF1

Organism
Tomato bushy stunt virus (strain Cherry) (TBSV)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

RNA-dependent RNA polymerase that plays an essential role in the virus replication.Curated

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein p92
Cleaved into the following chain:
Gene namesi
ORF Names:ORF1
OrganismiTomato bushy stunt virus (strain Cherry) (TBSV)
Taxonomic identifieri12147 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageTombusviridaeTombusvirus
Virus hostiCapsicum annuum (Bell pepper) [TaxID: 4072]
Malus [TaxID: 3749]
Pyrus (pears) [TaxID: 3766]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Solanum melongena (Eggplant) (Aubergine) [TaxID: 4111]
Tolmiea menziesii [TaxID: 29777]
Tulipa [TaxID: 13305]
ProteomesiUP000001666 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 818818RNA-directed RNA polymerasePRO_0000041336Add
BLAST
Chaini1 – 296296Protein p33PRO_0000041337Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP15962.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini524 – 641118RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR013707. Tombusvirus_p33.
[Graphical view]
PfamiPF00998. RdRP_3. 1 hit.
PF08500. Tombus_P33. 1 hit.
[Graphical view]
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METIKRMIWP KKEIFVGDFA TGVNRTVPVN IFQLVCRVVL RYMRTGKIEC
60 70 80 90 100
DSDSMTKFIV ELLKTDCAAK WEWFMKRRQR GDYIVPLSIA SIPIIPLLSY
110 120 130 140 150
ATRVRAVSVK AFGNELSFNV RVPRPSVPKK GLLLRLAAGL ALAPICALAV
160 170 180 190 200
YATLPREKLS VFKLRTEARA HMEDEREATD CLVVEPAREL KGKDGEDLLT
210 220 230 240 250
GSRLTKVIAS TGRPRRRPYA AKIAQVARAK VGYLKNSPEN RLIYQRVMIE
260 270 280 290 300
IMDKDCVRYV DRDVILPLAI GCCFVYPDGV EESAALWGSQ ESLGVKXGGL
310 320 330 340 350
VRLPGVVTQI NRDIPSDVLL PQEVLEVRTG PPNAKDRNIF MVAGCPSQAR
360 370 380 390 400
FLVHNHCLKN LKRGLVERVF CVERNGKLAR TPQPTKGAFG RLSPFRKAVC
410 420 430 440 450
EKVGVAHRLG YDGFLSYYSG AKLRTYTRAV ESLHITPVSE RDSHLTTFVK
460 470 480 490 500
AEKISTSKGD PAPRVIQPRN PRYNVELGRY LRHMESKLMK AVDGVFGETT
510 520 530 540 550
CIKGYTADEV GAIFRAKWDR FDKPVAIGLD ASRFDQHCSV EALQYEHSFY
560 570 580 590 600
RAMYPGNKLL GKLLEWQLHN KGKGYVPDGT ITYRKEGCRM SGDINTSLGN
610 620 630 640 650
YLLMCAMVHG YMRHLGINEF SLANCGDDCV LIVERRNLKQ IQRTLPEYFL
660 670 680 690 700
NLGYTMKVEQ PVFQLEEVEF CQAHPVQFQG GWKMVRNVRT AMSKDVHCVN
710 720 730 740 750
NIRDLATRRA WSNAQHHGGL ALSAGIPVVE TFYSRFKLYD VPRKHQRIDT
760 770 780 790 800
VTNVHKWRGS GGSYVVTPES RASFWAAFGL TGDEQLALED RLERWEMDLF
810
GEEGVDAHEP SILDSAVA
Length:818
Mass (Da):92,253
Last modified:October 5, 2010 - v2
Checksum:iD61AD04D5F94BC42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21958 Genomic RNA. Translation: AAB02534.1.
M21958 Genomic RNA. Translation: AAB02535.1.
PIRiA35315. RRVGCT.
RefSeqiNP_062897.1. NC_001554.1.
NP_062898.1. NC_001554.1.

Genome annotation databases

GeneIDi1493955.
1493956.
KEGGivg:1493955.
vg:1493956.

Keywords - Coding sequence diversityi

RNA suppression of termination

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21958 Genomic RNA. Translation: AAB02534.1.
M21958 Genomic RNA. Translation: AAB02535.1.
PIRiA35315. RRVGCT.
RefSeqiNP_062897.1. NC_001554.1.
NP_062898.1. NC_001554.1.

3D structure databases

ProteinModelPortaliP15962.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1493955.
1493956.
KEGGivg:1493955.
vg:1493956.

Family and domain databases

InterProiIPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR013707. Tombusvirus_p33.
[Graphical view]
PfamiPF00998. RdRP_3. 1 hit.
PF08500. Tombus_P33. 1 hit.
[Graphical view]
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome structure and synthesis of infectious RNA from clones of tomato bushy stunt virus."
    Hearne P.Q., Knorr D.A., Hillman B.I., Morris T.J.
    Virology 177:141-151(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiRDRP_TBSVC
AccessioniPrimary (citable) accession number: P15962
Secondary accession number(s): Q88485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 5, 2010
Last modified: April 1, 2015
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Readthrough of the terminator UAG occurs at position 297.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.