P15962 (RDRP_TBSVC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase
EC=2.7.7.48

Alternative name(s):
Protein p92

Cleaved into the following chain:

1. Protein p33

Gene names

ORF Names:

ORF1

Organism

Tomato bushy stunt virus (strain Cherry) (TBSV) [Reference proteome]

Taxonomic identifier

12147 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Tombusviridae › Tombusvirus › Tomato bushy stunt virus

Virus host

Capsicum annuum (Bell pepper) [TaxID: 4072]
Malus [TaxID: 3749]
Pyrus (pears) [TaxID: 3766]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Solanum melongena (Eggplant) (Aubergine) [TaxID: 4111]
Tolmiea menziesii [TaxID: 29777]
Tulipa [TaxID: 13305]

Protein attributes

Sequence length	818 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase that plays an essential role in the virus replication Probable.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Miscellaneous	Readthrough of the terminator UAG occurs at position 297.
Sequence similarities	Belongs to the tombusviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Coding sequence diversity	RNA suppression of termination
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 818	818	RNA-directed RNA polymerase		PRO_0000041336
	Chain	1 – 296	296	Protein p33		PRO_0000041337
Regions
	Domain	524 – 641	118	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P15962 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: D61AD04D5F94BC42	FASTA	818	92,253
	10 20 30 40 50 60 METIKRMIWP KKEIFVGDFA TGVNRTVPVN IFQLVCRVVL RYMRTGKIEC DSDSMTKFIV 70 80 90 100 110 120 ELLKTDCAAK WEWFMKRRQR GDYIVPLSIA SIPIIPLLSY ATRVRAVSVK AFGNELSFNV 130 140 150 160 170 180 RVPRPSVPKK GLLLRLAAGL ALAPICALAV YATLPREKLS VFKLRTEARA HMEDEREATD 190 200 210 220 230 240 CLVVEPAREL KGKDGEDLLT GSRLTKVIAS TGRPRRRPYA AKIAQVARAK VGYLKNSPEN 250 260 270 280 290 300 RLIYQRVMIE IMDKDCVRYV DRDVILPLAI GCCFVYPDGV EESAALWGSQ ESLGVKXGGL 310 320 330 340 350 360 VRLPGVVTQI NRDIPSDVLL PQEVLEVRTG PPNAKDRNIF MVAGCPSQAR FLVHNHCLKN 370 380 390 400 410 420 LKRGLVERVF CVERNGKLAR TPQPTKGAFG RLSPFRKAVC EKVGVAHRLG YDGFLSYYSG 430 440 450 460 470 480 AKLRTYTRAV ESLHITPVSE RDSHLTTFVK AEKISTSKGD PAPRVIQPRN PRYNVELGRY 490 500 510 520 530 540 LRHMESKLMK AVDGVFGETT CIKGYTADEV GAIFRAKWDR FDKPVAIGLD ASRFDQHCSV 550 560 570 580 590 600 EALQYEHSFY RAMYPGNKLL GKLLEWQLHN KGKGYVPDGT ITYRKEGCRM SGDINTSLGN 610 620 630 640 650 660 YLLMCAMVHG YMRHLGINEF SLANCGDDCV LIVERRNLKQ IQRTLPEYFL NLGYTMKVEQ 670 680 690 700 710 720 PVFQLEEVEF CQAHPVQFQG GWKMVRNVRT AMSKDVHCVN NIRDLATRRA WSNAQHHGGL 730 740 750 760 770 780 ALSAGIPVVE TFYSRFKLYD VPRKHQRIDT VTNVHKWRGS GGSYVVTPES RASFWAAFGL 790 800 810 TGDEQLALED RLERWEMDLF GEEGVDAHEP SILDSAVA

References

[1]	"The complete genome structure and synthesis of infectious RNA from clones of tomato bushy stunt virus." Hearne P.Q., Knorr D.A., Hillman B.I., Morris T.J. Virology 177:141-151(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M21958 Genomic RNA. Translation: AAB02534.1. M21958 Genomic RNA. Translation: AAB02535.1.
PIR	RRVGCT. A35315.
RefSeq	NP_062897.1. NC_001554.1. NP_062898.1. NC_001554.1.
3D structure databases
ProteinModelPortal	P15962.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1493955. 1493956.
Family and domain databases
InterPro	IPR007094. RNA-dir_pol_PSvirus. IPR002166. RNA_pol_HCV. IPR013707. Tombusvirus_p33. [Graphical view]
Pfam	PF00998. RdRP_3. 1 hit. PF08500. Tombus_P33. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_TBSVC

Accession

Primary (citable) accession number: P15962
Secondary accession number(s): Q88485

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	October 5, 2010
Last modified:	April 3, 2013
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program