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P15950

- KLK3_RAT

UniProt

P15950 - KLK3_RAT

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Protein

Glandular kallikrein-3, submandibular

Gene

Klk3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei47 – 471Charge relay system
Active sitei140 – 1401Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.77. 5301.

Protein family/group databases

MEROPSiS01.411.

Names & Taxonomyi

Protein namesi
Recommended name:
Glandular kallikrein-3, submandibular (EC:3.4.21.35)
Short name:
rGK-3
Alternative name(s):
RSGK-50
S1 kallikrein
Tissue kallikrein
Gene namesi
Name:Klk3
Synonyms:Klk-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 188›188Glandular kallikrein-3, submandibularPRO_0000088702Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi10 – 101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi79 ↔ 146PROSITE-ProRule annotation
Disulfide bondi111 ↔ 125PROSITE-ProRule annotation
Disulfide bondi136 ↔ 161PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP15950.

Expressioni

Gene expression databases

GenevestigatoriP15950.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025777.

Structurei

3D structure databases

ProteinModelPortaliP15950.
SMRiP15950. Positions 1-188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini‹1 – 185›185Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15950.
OrthoDBiEOG7W9RV6.
PhylomeDBiP15950.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P15950-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
NYHVLLGQNN LSEDVQHRLV SQSFRHPDYK PFLMRNHTRK PKDYSNDLML
60 70 80 90 100
LHLSEPADIT DGVKVIDLPT KEPKVGSTCL VSGWGSTNPS EWEFPDDLQC
110 120 130 140 150
VNIHLLSNEK CIKAYKEKVT DLMLCAGELE GGKDTCRGDS GGPLICDGVL
160 170 180
QGITSWGSVP CGEPNKPGIY TKLIKFTSWI KEVMKENP
Length:188
Mass (Da):20,986
Last modified:June 1, 1994 - v2
Checksum:iB3ECEC65582EB1E9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti34 – 352MR → IW in AAA41465. (PubMed:2998455)Curated
Sequence conflicti42 – 421K → G in AAA41465. (PubMed:2998455)Curated
Sequence conflicti186 – 1861E → K in AAA42080. (PubMed:2550051)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26534 Genomic DNA. Translation: AAA42080.1.
M11564 mRNA. Translation: AAA41465.1.
PIRiB23863.
B32340.
UniGeneiRn.107068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26534 Genomic DNA. Translation: AAA42080.1 .
M11564 mRNA. Translation: AAA41465.1 .
PIRi B23863.
B32340.
UniGenei Rn.107068.

3D structure databases

ProteinModelPortali P15950.
SMRi P15950. Positions 1-188.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000025777.

Protein family/group databases

MEROPSi S01.411.

Proteomic databases

PRIDEi P15950.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P15950.
OrthoDBi EOG7W9RV6.
PhylomeDBi P15950.
TreeFami TF331065.

Enzyme and pathway databases

BRENDAi 3.4.21.77. 5301.

Gene expression databases

Genevestigatori P15950.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of genes encoding rat tonin and a kallikrein-like serine protease."
    Shai S.Y., Woodley-Miller C., Chao J., Chao L.
    Biochemistry 28:5334-5343(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."
    Ashley P.L., MacDonald R.J.
    Biochemistry 24:4512-4520(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-188.

Entry informationi

Entry nameiKLK3_RAT
AccessioniPrimary (citable) accession number: P15950
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 1994
Last modified: October 1, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3