Reviewed, UniProtKB/Swiss-Prot P15950 (KLK3_RAT)

Names and origin

Protein names

Recommended name:
    Glandular kallikrein-3, submandibular
    EC=3.4.21.35
Alternative name(s):
    Tissue kallikrein
    S1 kallikrein
    RGK-3
    RSGK-50

Gene names

Name:	Klk3
Synonyms:	Klk-3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	188 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 188	›188	Glandular kallikrein-3, submandibular		PRO_0000088702
Regions
	Domain	‹1 – 185	›185	Peptidase S1
Sites
	Active site	47	1	Charge relay system
	Active site	140	1	Charge relay system
Amino acid modifications
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Glycosylation	36	1	N-linked (GlcNAc...) Potential
	Disulfide bond	79 ↔ 146		By similarity
	Disulfide bond	111 ↔ 125		By similarity
	Disulfide bond	136 ↔ 161		By similarity
Experimental info
	Sequence conflict	34 – 35	2	MR → IW in AAA41465. Ref.2
	Sequence conflict	42	1	K → G in AAA41465. Ref.2
	Sequence conflict	186	1	E → K in AAA42080. Ref.1
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P15950-1 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: B3ECEC65582EB1E9	FASTA	188	20,986
	10 20 30 40 50 60 NYHVLLGQNN LSEDVQHRLV SQSFRHPDYK PFLMRNHTRK PKDYSNDLML LHLSEPADIT 70 80 90 100 110 120 DGVKVIDLPT KEPKVGSTCL VSGWGSTNPS EWEFPDDLQC VNIHLLSNEK CIKAYKEKVT 130 140 150 160 170 180 DLMLCAGELE GGKDTCRGDS GGPLICDGVL QGITSWGSVP CGEPNKPGIY TKLIKFTSWI KEVMKENP

References

[1]	"Characterization of genes encoding rat tonin and a kallikrein-like serine protease." Shai S.Y., Woodley-Miller C., Chao J., Chao L. Biochemistry 28:5334-5343(1989) [PubMed: 2550051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin." Ashley P.L., MacDonald R.J. Biochemistry 24:4512-4520(1985) [PubMed: 2998455] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-188.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M26534 Genomic DNA. Translation: AAA42080.1. M11564 mRNA. Translation: AAA41465.1.
IPI	IPI00778532.
PIR	B23863. B32340.
UniGene	Rn.107068
3D structure databases
HSSP	HSSP built from PDB template 1TON based on UniProtKB P00759.
SMR	P15950. Positions 1-188.
ModBase	Search...
Protein family/group databases
MEROPS	S01.411.
Genome annotation databases
Ensembl	ENSRNOG00000033414. Rattus norvegicus. [Contig view]
Phylogenomic databases
HOVERGEN	P15950.
Enzyme and pathway databases
BRENDA	3.4.21.35. 248. 3.4.21.77. 248.
Gene expression databases
ArrayExpress	P15950.
GermOnline	ENSRNOG00000033414. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. Partial match. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KLK3_RAT

Accession

Primary (citable) accession number: P15950

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	June 1, 1994
Last modified:	March 3, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)