Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glandular kallikrein-3, submandibular

Gene

Klk3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei47 – 471Charge relay system
Active sitei140 – 1401Charge relay system

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.77. 5301.

Protein family/group databases

MEROPSiS01.411.

Names & Taxonomyi

Protein namesi
Recommended name:
Glandular kallikrein-3, submandibular (EC:3.4.21.35)
Short name:
rGK-3
Alternative name(s):
RSGK-50
S1 kallikrein
Tissue kallikrein
Gene namesi
Name:Klk3
Synonyms:Klk-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 188›188Glandular kallikrein-3, submandibularPRO_0000088702Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi10 – 101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi79 ↔ 146PROSITE-ProRule annotation
Disulfide bondi111 ↔ 125PROSITE-ProRule annotation
Disulfide bondi136 ↔ 161PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP15950.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025777.

Structurei

3D structure databases

ProteinModelPortaliP15950.
SMRiP15950. Positions 1-188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini‹1 – 185›185Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15950.
OrthoDBiEOG7W9RV6.
PhylomeDBiP15950.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P15950-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
NYHVLLGQNN LSEDVQHRLV SQSFRHPDYK PFLMRNHTRK PKDYSNDLML
60 70 80 90 100
LHLSEPADIT DGVKVIDLPT KEPKVGSTCL VSGWGSTNPS EWEFPDDLQC
110 120 130 140 150
VNIHLLSNEK CIKAYKEKVT DLMLCAGELE GGKDTCRGDS GGPLICDGVL
160 170 180
QGITSWGSVP CGEPNKPGIY TKLIKFTSWI KEVMKENP
Length:188
Mass (Da):20,986
Last modified:June 1, 1994 - v2
Checksum:iB3ECEC65582EB1E9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti34 – 352MR → IW in AAA41465 (PubMed:2998455).Curated
Sequence conflicti42 – 421K → G in AAA41465 (PubMed:2998455).Curated
Sequence conflicti186 – 1861E → K in AAA42080 (PubMed:2550051).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26534 Genomic DNA. Translation: AAA42080.1.
M11564 mRNA. Translation: AAA41465.1.
PIRiB23863.
B32340.
UniGeneiRn.107068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26534 Genomic DNA. Translation: AAA42080.1.
M11564 mRNA. Translation: AAA41465.1.
PIRiB23863.
B32340.
UniGeneiRn.107068.

3D structure databases

ProteinModelPortaliP15950.
SMRiP15950. Positions 1-188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025777.

Protein family/group databases

MEROPSiS01.411.

Proteomic databases

PRIDEiP15950.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15950.
OrthoDBiEOG7W9RV6.
PhylomeDBiP15950.
TreeFamiTF331065.

Enzyme and pathway databases

BRENDAi3.4.21.77. 5301.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of genes encoding rat tonin and a kallikrein-like serine protease."
    Shai S.Y., Woodley-Miller C., Chao J., Chao L.
    Biochemistry 28:5334-5343(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."
    Ashley P.L., MacDonald R.J.
    Biochemistry 24:4512-4520(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-188.

Entry informationi

Entry nameiKLK3_RAT
AccessioniPrimary (citable) accession number: P15950
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 1994
Last modified: June 24, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.