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P15950 (KLK3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glandular kallikrein-3, submandibular

Short name=rGK-3
EC=3.4.21.35
Alternative name(s):
RSGK-50
S1 kallikrein
Tissue kallikrein
Gene names
Name:Klk3
Synonyms:Klk-3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length188 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 188›188Glandular kallikrein-3, submandibular
PRO_0000088702

Regions

Domain‹1 – 185›185Peptidase S1

Sites

Active site471Charge relay system
Active site1401Charge relay system

Amino acid modifications

Glycosylation101N-linked (GlcNAc...) Potential
Glycosylation361N-linked (GlcNAc...) Potential
Disulfide bond79 ↔ 146 By similarity
Disulfide bond111 ↔ 125 By similarity
Disulfide bond136 ↔ 161 By similarity

Experimental info

Sequence conflict34 – 352MR → IW in AAA41465. Ref.2
Sequence conflict421K → G in AAA41465. Ref.2
Sequence conflict1861E → K in AAA42080. Ref.1
Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P15950 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: B3ECEC65582EB1E9

FASTA18820,986
        10         20         30         40         50         60 
NYHVLLGQNN LSEDVQHRLV SQSFRHPDYK PFLMRNHTRK PKDYSNDLML LHLSEPADIT 

        70         80         90        100        110        120 
DGVKVIDLPT KEPKVGSTCL VSGWGSTNPS EWEFPDDLQC VNIHLLSNEK CIKAYKEKVT 

       130        140        150        160        170        180 
DLMLCAGELE GGKDTCRGDS GGPLICDGVL QGITSWGSVP CGEPNKPGIY TKLIKFTSWI 


KEVMKENP 

« Hide

References

[1]"Characterization of genes encoding rat tonin and a kallikrein-like serine protease."
Shai S.Y., Woodley-Miller C., Chao J., Chao L.
Biochemistry 28:5334-5343(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."
Ashley P.L., MacDonald R.J.
Biochemistry 24:4512-4520(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-188.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M26534 Genomic DNA. Translation: AAA42080.1.
M11564 mRNA. Translation: AAA41465.1.
PIRB23863.
B32340.
UniGeneRn.107068.

3D structure databases

ProteinModelPortalP15950.
SMRP15950. Positions 1-188.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000025777.

Protein family/group databases

MEROPSS01.411.

Proteomic databases

PRIDEP15950.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP15950.
OrthoDBEOG7W9RV6.
PhylomeDBP15950.
TreeFamTF331065.

Enzyme and pathway databases

BRENDA3.4.21.77. 5301.

Gene expression databases

GenevestigatorP15950.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKLK3_RAT
AccessionPrimary (citable) accession number: P15950
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries