ID   K1KB9_MOUSE             Reviewed;         261 AA.
AC   P15949;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   24-JAN-2024, entry version 182.
DE   RecName: Full=Kallikrein 1-related peptidase b9;
DE            EC=3.4.21.35;
DE   AltName: Full=Epidermal growth factor-binding protein type C;
DE            Short=EGF-BP C;
DE   AltName: Full=Glandular kallikrein K9;
DE            Short=mGK-9;
DE   AltName: Full=Tissue kallikrein-9;
DE   Flags: Precursor;
GN   Name=Klk1b9; Synonyms=Egfbp3, Klk-9, Klk9;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=3322386; DOI=10.1021/bi00395a025;
RA   Blaber M., Isackson P.J., Bradshaw R.A.;
RT   "A complete cDNA sequence for the major epidermal growth factor binding
RT   protein in the male mouse submandibular gland.";
RL   Biochemistry 26:6742-6749(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BALB/cJ; TISSUE=Salivary gland;
RX   PubMed=3322387; DOI=10.1021/bi00395a026;
RA   Drinkwater C.C., Evans B.A., Richards R.I.;
RT   "Mouse glandular kallikrein genes: identification and characterization of
RT   the genes encoding the epidermal growth factor binding proteins.";
RL   Biochemistry 26:6750-6756(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Salivary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE OF 16-54 AND 70-122.
RC   STRAIN=BALB/cJ; TISSUE=Liver;
RX   PubMed=3036794; DOI=10.1016/s0021-9258(18)47521-7;
RA   Evans B.A., Drinkwater C.C., Richards R.I.;
RT   "Mouse glandular kallikrein genes. Structure and partial sequence analysis
RT   of the kallikrein gene locus.";
RL   J. Biol. Chem. 262:8027-8034(1987).
CC   -!- FUNCTION: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in
CC       kininogen to release Lys-bradykinin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Preferential cleavage of Arg-|-Xaa bonds in small molecule
CC         substrates. Highly selective action to release kallidin (lysyl-
CC         bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-
CC         Xaa.; EC=3.4.21.35;
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. Kallikrein subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00274}.
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DR   EMBL; M17962; AAA37541.1; -; mRNA.
DR   EMBL; M17985; AAA37681.1; -; Genomic_DNA.
DR   EMBL; M17983; AAA37681.1; JOINED; Genomic_DNA.
DR   EMBL; M17984; AAA37681.1; JOINED; Genomic_DNA.
DR   EMBL; BC024624; AAH24624.1; -; mRNA.
DR   EMBL; BC048869; AAH48869.1; -; mRNA.
DR   EMBL; M18588; AAA39350.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; M18608; AAA39351.1; -; Genomic_DNA.
DR   CCDS; CCDS21191.1; -.
DR   PIR; C29746; A29745.
DR   PIR; I70014; I70014.
DR   RefSeq; NP_034246.1; NM_010116.1.
DR   AlphaFoldDB; P15949; -.
DR   SMR; P15949; -.
DR   STRING; 10090.ENSMUSP00000080133; -.
DR   MEROPS; S01.071; -.
DR   GlyCosmos; P15949; 1 site, No reported glycans.
DR   GlyGen; P15949; 1 site.
DR   MaxQB; P15949; -.
DR   PaxDb; 10090-ENSMUSP00000080133; -.
DR   ProteomicsDB; 269141; -.
DR   DNASU; 13648; -.
DR   Ensembl; ENSMUST00000081399.4; ENSMUSP00000080133.4; ENSMUSG00000059042.4.
DR   GeneID; 13648; -.
DR   KEGG; mmu:13648; -.
DR   UCSC; uc009god.1; mouse.
DR   AGR; MGI:95293; -.
DR   CTD; 13648; -.
DR   MGI; MGI:95293; Klk1b9.
DR   VEuPathDB; HostDB:ENSMUSG00000059042; -.
DR   eggNOG; KOG3627; Eukaryota.
DR   GeneTree; ENSGT01020000230389; -.
DR   HOGENOM; CLU_006842_1_1_1; -.
DR   InParanoid; P15949; -.
DR   OMA; WLENDIS; -.
DR   OrthoDB; 2495122at2759; -.
DR   PhylomeDB; P15949; -.
DR   TreeFam; TF331065; -.
DR   BRENDA; 3.4.21.B40; 3474.
DR   BioGRID-ORCS; 13648; 3 hits in 75 CRISPR screens.
DR   ChiTaRS; Klk1b9; mouse.
DR   PRO; PR:P15949; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; P15949; Protein.
DR   Bgee; ENSMUSG00000059042; Expressed in male reproductive system and 10 other cell types or tissues.
DR   GO; GO:0001669; C:acrosomal vesicle; ISO:MGI.
DR   GO; GO:0045177; C:apical part of cell; ISO:MGI.
DR   GO; GO:0005615; C:extracellular space; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; ISO:MGI.
DR   GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR   GO; GO:0030141; C:secretory granule; IBA:GO_Central.
DR   GO; GO:0004175; F:endopeptidase activity; ISO:MGI.
DR   GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; ISO:MGI.
DR   GO; GO:0008233; F:peptidase activity; IDA:MGI.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; ISO:MGI.
DR   GO; GO:0008236; F:serine-type peptidase activity; ISO:MGI.
DR   GO; GO:0003073; P:regulation of systemic arterial blood pressure; IBA:GO_Central.
DR   GO; GO:0031638; P:zymogen activation; IDA:MGI.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; Trypsin-like serine proteases; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR018114; TRYPSIN_HIS.
DR   InterPro; IPR033116; TRYPSIN_SER.
DR   PANTHER; PTHR24271:SF47; KALLIKREIN-1; 1.
DR   PANTHER; PTHR24271; KALLIKREIN-RELATED; 1.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
DR   Genevisible; P15949; MM.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Hydrolase; Protease; Reference proteome;
KW   Serine protease; Signal; Zymogen.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000305"
FT   PROPEP          19..24
FT                   /note="Activation peptide"
FT                   /id="PRO_0000027979"
FT   CHAIN           25..261
FT                   /note="Kallikrein 1-related peptidase b9"
FT                   /id="PRO_0000027980"
FT   DOMAIN          25..258
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        65
FT                   /note="Charge relay system"
FT   ACT_SITE        120
FT                   /note="Charge relay system"
FT   ACT_SITE        213
FT                   /note="Charge relay system"
FT   CARBOHYD        102
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000305"
FT   DISULFID        31..173
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        50..66
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        152..219
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        184..198
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        209..234
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
SQ   SEQUENCE   261 AA;  28900 MW;  796FD4DAE56020D7 CRC64;
     MRFLILFLAL SLGGIDAAPP VHSRIVGGFK CEKNSQPWHV AVYRYNEYIC GGVLLDANWV
     LTAAHCYYEE NKVSLGKNNL YEEEPSAQHR LVSKSFLHPG YNRSLHRNHI RHPEYDYSND
     LMLLRLSKPA DITDVVKPIA LPTEEPKLGS TCLASGWGST TPFKFQNAKD LQCVNLKLLP
     NEDCGKAHIE KVTDVMLCAG ETDGGKDTCK GDSGGPLICD GVLQGITSWG FTPCGEPKKP
     GVYTKLIKFT SWIKDTMAKN L
//