P15949 (K1KB9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kallikrein 1-related peptidase b9
EC=3.4.21.35

Alternative name(s):
Epidermal growth factor-binding protein type C
Short name=EGF-BP C
Glandular kallikrein K9
Short name=mGK-9
Tissue kallikrein-9

Gene names

Name:	Klk1b9
Synonyms:	Egfbp3, Klk-9, Klk9

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide		PRO_0000027979
	Chain	25 – 261	237	Kallikrein 1-related peptidase b9		PRO_0000027980
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system
	Active site	120	1	Charge relay system
	Active site	213	1	Charge relay system
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Probable
	Disulfide bond	31 ↔ 173		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	152 ↔ 219		By similarity
	Disulfide bond	184 ↔ 198		By similarity
	Disulfide bond	209 ↔ 234		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15949 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 796FD4DAE56020D7	FASTA	261	28,900
	10 20 30 40 50 60 MRFLILFLAL SLGGIDAAPP VHSRIVGGFK CEKNSQPWHV AVYRYNEYIC GGVLLDANWV 70 80 90 100 110 120 LTAAHCYYEE NKVSLGKNNL YEEEPSAQHR LVSKSFLHPG YNRSLHRNHI RHPEYDYSND 130 140 150 160 170 180 LMLLRLSKPA DITDVVKPIA LPTEEPKLGS TCLASGWGST TPFKFQNAKD LQCVNLKLLP 190 200 210 220 230 240 NEDCGKAHIE KVTDVMLCAG ETDGGKDTCK GDSGGPLICD GVLQGITSWG FTPCGEPKKP 250 260 GVYTKLIKFT SWIKDTMAKN L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A complete cDNA sequence for the major epidermal growth factor binding protein in the male mouse submandibular gland." Blaber M., Isackson P.J., Bradshaw R.A. Biochemistry 26:6742-6749(1987) [PubMed: 3322386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mouse glandular kallikrein genes: identification and characterization of the genes encoding the epidermal growth factor binding proteins." Drinkwater C.C., Evans B.A., Richards R.I. Biochemistry 26:6750-6756(1987) [PubMed: 3322387] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Salivary gland.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland.
[4]	"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus." Evans B.A., Drinkwater C.C., Richards R.I. J. Biol. Chem. 262:8027-8034(1987) [PubMed: 3036794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 16-54 AND 70-122. Strain: BALB/c. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M17962 mRNA. Translation: AAA37541.1. M17985, M17983, M17984 Genomic DNA. Translation: AAA37681.1. BC024624 mRNA. Translation: AAH24624.1. BC048869 mRNA. Translation: AAH48869.1. M18588 Genomic DNA. Translation: AAA39350.1. Sequence problems. M18608 Genomic DNA. Translation: AAA39351.1.
IPI	IPI00136040.
PIR	A29745. C29746. I70014.
RefSeq	NP_034246.1. NM_010116.1.
UniGene	Mm.440884.
3D structure databases
ProteinModelPortal	P15949.
SMR	P15949. Positions 25-260.
ModBase	Search...
Protein-protein interaction databases
STRING	P15949.
Protein family/group databases
MEROPS	S01.071.
Proteomic databases
PRIDE	P15949.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000081399; ENSMUSP00000080133; ENSMUSG00000059042.
GeneID	13648.
KEGG	mmu:13648.
UCSC	uc009god.1. mouse.
Organism-specific databases
CTD	13648.
MGI	MGI:95293. Klk1b9.
Phylogenomic databases
eggNOG	roNOG16199.
GeneTree	ENSGT00590000082811.
HOGENOM	HBG755338.
HOVERGEN	HBG013304.
InParanoid	P15949.
OMA	IINEYLC.
OrthoDB	EOG4DZ1VW.
PhylomeDB	P15949.
Gene expression databases
ArrayExpress	P15949.
Bgee	P15949.
CleanEx	MM_KLK1B9.
Genevestigator	P15949.
GermOnline	ENSMUSG00000059042. Mus musculus.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
KO	K01325.
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	284370.
SOURCE	Search...

Entry information

Entry name

K1KB9_MOUSE

Accession

Primary (citable) accession number: P15949

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	November 16, 2011
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program