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Protein

Kallikrein 1-related peptidase b9

Gene

Klk1b9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei120 – 1201Charge relay system
Active sitei213 – 2131Charge relay system

GO - Molecular functioni

  1. peptidase activity Source: MGI
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. zymogen activation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.
REACT_235886. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiS01.071.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b9 (EC:3.4.21.35)
Alternative name(s):
Epidermal growth factor-binding protein type C
Short name:
EGF-BP C
Glandular kallikrein K9
Short name:
mGK-9
Tissue kallikrein-9
Gene namesi
Name:Klk1b9
Synonyms:Egfbp3, Klk-9, Klk9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:95293. Klk1b9.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818CuratedAdd
BLAST
Propeptidei19 – 246Activation peptidePRO_0000027979
Chaini25 – 261237Kallikrein 1-related peptidase b9PRO_0000027980Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 173PROSITE-ProRule annotation
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Glycosylationi102 – 1021N-linked (GlcNAc...)Curated
Disulfide bondi152 ↔ 219PROSITE-ProRule annotation
Disulfide bondi184 ↔ 198PROSITE-ProRule annotation
Disulfide bondi209 ↔ 234PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP15949.
PaxDbiP15949.
PRIDEiP15949.

Expressioni

Gene expression databases

BgeeiP15949.
CleanExiMM_KLK1B9.
GenevestigatoriP15949.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000080133.

Structurei

3D structure databases

ProteinModelPortaliP15949.
SMRiP15949. Positions 25-260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 258234Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15949.
KOiK01325.
OMAiIINEYLC.
OrthoDBiEOG75B84T.
PhylomeDBiP15949.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15949-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFLILFLAL SLGGIDAAPP VHSRIVGGFK CEKNSQPWHV AVYRYNEYIC
60 70 80 90 100
GGVLLDANWV LTAAHCYYEE NKVSLGKNNL YEEEPSAQHR LVSKSFLHPG
110 120 130 140 150
YNRSLHRNHI RHPEYDYSND LMLLRLSKPA DITDVVKPIA LPTEEPKLGS
160 170 180 190 200
TCLASGWGST TPFKFQNAKD LQCVNLKLLP NEDCGKAHIE KVTDVMLCAG
210 220 230 240 250
ETDGGKDTCK GDSGGPLICD GVLQGITSWG FTPCGEPKKP GVYTKLIKFT
260
SWIKDTMAKN L
Length:261
Mass (Da):28,900
Last modified:April 1, 1990 - v1
Checksum:i796FD4DAE56020D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17962 mRNA. Translation: AAA37541.1.
M17985, M17983, M17984 Genomic DNA. Translation: AAA37681.1.
BC024624 mRNA. Translation: AAH24624.1.
BC048869 mRNA. Translation: AAH48869.1.
M18588 Genomic DNA. Translation: AAA39350.1. Sequence problems.
M18608 Genomic DNA. Translation: AAA39351.1.
CCDSiCCDS21191.1.
PIRiC29746. A29745.
I70014.
RefSeqiNP_034246.1. NM_010116.1.
UniGeneiMm.440884.

Genome annotation databases

EnsembliENSMUST00000081399; ENSMUSP00000080133; ENSMUSG00000059042.
GeneIDi13648.
KEGGimmu:13648.
UCSCiuc009god.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17962 mRNA. Translation: AAA37541.1.
M17985, M17983, M17984 Genomic DNA. Translation: AAA37681.1.
BC024624 mRNA. Translation: AAH24624.1.
BC048869 mRNA. Translation: AAH48869.1.
M18588 Genomic DNA. Translation: AAA39350.1. Sequence problems.
M18608 Genomic DNA. Translation: AAA39351.1.
CCDSiCCDS21191.1.
PIRiC29746. A29745.
I70014.
RefSeqiNP_034246.1. NM_010116.1.
UniGeneiMm.440884.

3D structure databases

ProteinModelPortaliP15949.
SMRiP15949. Positions 25-260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000080133.

Protein family/group databases

MEROPSiS01.071.

Proteomic databases

MaxQBiP15949.
PaxDbiP15949.
PRIDEiP15949.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081399; ENSMUSP00000080133; ENSMUSG00000059042.
GeneIDi13648.
KEGGimmu:13648.
UCSCiuc009god.1. mouse.

Organism-specific databases

CTDi13648.
MGIiMGI:95293. Klk1b9.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15949.
KOiK01325.
OMAiIINEYLC.
OrthoDBiEOG75B84T.
PhylomeDBiP15949.
TreeFamiTF331065.

Enzyme and pathway databases

ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.
REACT_235886. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

NextBioi284370.
PROiP15949.
SOURCEiSearch...

Gene expression databases

BgeeiP15949.
CleanExiMM_KLK1B9.
GenevestigatoriP15949.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A complete cDNA sequence for the major epidermal growth factor binding protein in the male mouse submandibular gland."
    Blaber M., Isackson P.J., Bradshaw R.A.
    Biochemistry 26:6742-6749(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Mouse glandular kallikrein genes: identification and characterization of the genes encoding the epidermal growth factor binding proteins."
    Drinkwater C.C., Evans B.A., Richards R.I.
    Biochemistry 26:6750-6756(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
    Tissue: Salivary gland.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  4. "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
    Evans B.A., Drinkwater C.C., Richards R.I.
    J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 16-54 AND 70-122.
    Strain: BALB/c.
    Tissue: Liver.

Entry informationi

Entry nameiK1KB9_MOUSE
AccessioniPrimary (citable) accession number: P15949
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: February 4, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.