ID   K1B22_MOUSE             Reviewed;         259 AA.
AC   P15948;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   27-MAR-2024, entry version 174.
DE   RecName: Full=Kallikrein 1-related peptidase b22;
DE            EC=3.4.21.35;
DE   AltName: Full=Beta-NGF-endopeptidase;
DE   AltName: Full=Epidermal growth factor-binding protein type A;
DE            Short=EGF-BP A;
DE   AltName: Full=Glandular kallikrein K22;
DE            Short=mGK-22;
DE   AltName: Full=Nerve growth factor beta chain endopeptidase;
DE   AltName: Full=Tissue kallikrein 22;
DE   Flags: Precursor;
GN   Name=Klk1b22; Synonyms=Klk-22, Klk22;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BALB/cJ; TISSUE=Salivary gland;
RX   PubMed=3322387; DOI=10.1021/bi00395a026;
RA   Drinkwater C.C., Evans B.A., Richards R.I.;
RT   "Mouse glandular kallikrein genes: identification and characterization of
RT   the genes encoding the epidermal growth factor binding proteins.";
RL   Biochemistry 26:6750-6756(1987).
RN   [2]
RP   PROTEIN SEQUENCE OF 25-54.
RX   PubMed=2012805; DOI=10.1021/bi00228a014;
RA   Fahnestock M., Woo J.E., Lopez G.A., Snow J., Walz D.A., Arici M.J.,
RA   Mobley W.C.;
RT   "Beta-NGF-endopeptidase: structure and activity of a kallikrein encoded by
RT   the gene mGK-22.";
RL   Biochemistry 30:3443-3450(1991).
RN   [3]
RP   PROTEIN SEQUENCE OF 25-41.
RC   TISSUE=Submandibular gland;
RX   PubMed=1639762; DOI=10.1093/oxfordjournals.jbchem.a123812;
RA   Peters J., Takahashi S., Tada M., Miyake Y.;
RT   "mGK-6-derived true tissue kallikrein is synthesized, processed, and
RT   targeted through a regulated secretory pathway in mouse pituitary AtT-20
RT   cells.";
RL   J. Biochem. 111:643-648(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-54 AND 70-120.
RX   PubMed=3036794; DOI=10.1016/s0021-9258(18)47521-7;
RA   Evans B.A., Drinkwater C.C., Richards R.I.;
RT   "Mouse glandular kallikrein genes. Structure and partial sequence analysis
RT   of the kallikrein gene locus.";
RL   J. Biol. Chem. 262:8027-8034(1987).
CC   -!- FUNCTION: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in
CC       kininogen to release Lys-bradykinin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Preferential cleavage of Arg-|-Xaa bonds in small molecule
CC         substrates. Highly selective action to release kallidin (lysyl-
CC         bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-
CC         Xaa.; EC=3.4.21.35;
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. Kallikrein subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00274}.
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DR   EMBL; M17979; AAA37682.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; M17977; AAA37682.1; JOINED; Genomic_DNA.
DR   EMBL; M17978; AAA37682.1; JOINED; Genomic_DNA.
DR   EMBL; M18598; AAA39361.1; -; Genomic_DNA.
DR   EMBL; M18618; AAA39362.1; -; Genomic_DNA.
DR   CCDS; CCDS21196.1; -.
DR   PIR; A29746; A29746.
DR   RefSeq; NP_034244.1; NM_010114.2.
DR   AlphaFoldDB; P15948; -.
DR   SMR; P15948; -.
DR   STRING; 10090.ENSMUSP00000076733; -.
DR   MEROPS; S01.039; -.
DR   GlyCosmos; P15948; 1 site, No reported glycans.
DR   GlyGen; P15948; 1 site.
DR   PaxDb; 10090-ENSMUSP00000076733; -.
DR   ProteomicsDB; 269136; -.
DR   DNASU; 13646; -.
DR   Ensembl; ENSMUST00000077528.7; ENSMUSP00000076733.7; ENSMUSG00000060177.7.
DR   GeneID; 13646; -.
DR   KEGG; mmu:13646; -.
DR   UCSC; uc009goi.1; mouse.
DR   AGR; MGI:95291; -.
DR   CTD; 13646; -.
DR   MGI; MGI:95291; Klk1b22.
DR   VEuPathDB; HostDB:ENSMUSG00000060177; -.
DR   eggNOG; KOG3627; Eukaryota.
DR   GeneTree; ENSGT01020000230389; -.
DR   HOGENOM; CLU_006842_1_1_1; -.
DR   InParanoid; P15948; -.
DR   OMA; LCAGEMN; -.
DR   OrthoDB; 4629979at2759; -.
DR   PhylomeDB; P15948; -.
DR   TreeFam; TF331065; -.
DR   BioGRID-ORCS; 13646; 3 hits in 79 CRISPR screens.
DR   ChiTaRS; Klk1b22; mouse.
DR   PRO; PR:P15948; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; P15948; Protein.
DR   Bgee; ENSMUSG00000060177; Expressed in right kidney and 9 other cell types or tissues.
DR   ExpressionAtlas; P15948; baseline and differential.
DR   GO; GO:0001669; C:acrosomal vesicle; ISO:MGI.
DR   GO; GO:0045177; C:apical part of cell; ISO:MGI.
DR   GO; GO:0005615; C:extracellular space; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; ISO:MGI.
DR   GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR   GO; GO:0030141; C:secretory granule; IBA:GO_Central.
DR   GO; GO:0004175; F:endopeptidase activity; ISO:MGI.
DR   GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; ISO:MGI.
DR   GO; GO:0008233; F:peptidase activity; IDA:MGI.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; ISO:MGI.
DR   GO; GO:0008236; F:serine-type peptidase activity; ISO:MGI.
DR   GO; GO:0003073; P:regulation of systemic arterial blood pressure; IBA:GO_Central.
DR   GO; GO:0031638; P:zymogen activation; IDA:MGI.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; Trypsin-like serine proteases; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR018114; TRYPSIN_HIS.
DR   InterPro; IPR033116; TRYPSIN_SER.
DR   PANTHER; PTHR24271:SF47; KALLIKREIN-1; 1.
DR   PANTHER; PTHR24271; KALLIKREIN-RELATED; 1.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
DR   Genevisible; P15948; MM.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Hydrolase;
KW   Protease; Reference proteome; Serine protease; Signal; Zymogen.
FT   SIGNAL          1..17
FT   PROPEP          18..24
FT                   /note="Activation peptide"
FT                   /evidence="ECO:0000269|PubMed:1639762,
FT                   ECO:0000269|PubMed:2012805"
FT                   /id="PRO_0000027989"
FT   CHAIN           25..259
FT                   /note="Kallikrein 1-related peptidase b22"
FT                   /id="PRO_0000027990"
FT   DOMAIN          25..256
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        65
FT                   /note="Charge relay system"
FT   ACT_SITE        118
FT                   /note="Charge relay system"
FT   ACT_SITE        211
FT                   /note="Charge relay system"
FT   CARBOHYD        102
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000305"
FT   DISULFID        31..171
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        50..66
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        150..217
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        182..196
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        207..232
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
SQ   SEQUENCE   259 AA;  28384 MW;  D7745794D8A87B9C CRC64;
     MRFLILFLTL SLGGIDAAPP VQSRILGGFK CEKNSQPWQV AVYYLDEYLC GGVLLDRNWV
     LTAAHCYEDK YNIWLGKNKL FQDEPSAQHR LVSKSFPHPD FNMSLLQSVP TGADLSNDLM
     LLRLSKPADI TDVVKPIDLP TTEPKLGSTC LASGWGSINQ LIYQNPNDLQ CVSIKLHPNE
     VCVKAHILKV TDVMLCAGEM NGGKDTCKGD SGGPLICDGV LQGITSWGST PCGEPNAPAI
     YTKLIKFTSW IKDTMAKNP
//