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P15948

- K1B22_MOUSE

UniProt

P15948 - K1B22_MOUSE

Protein

Kallikrein 1-related peptidase b22

Gene

Klk1b22

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

    Catalytic activityi

    Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Charge relay system
    Active sitei118 – 1181Charge relay system
    Active sitei211 – 2111Charge relay system

    GO - Molecular functioni

    1. peptidase activity Source: MGI
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. zymogen activation Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.

    Protein family/group databases

    MEROPSiS01.039.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kallikrein 1-related peptidase b22 (EC:3.4.21.35)
    Alternative name(s):
    Beta-NGF-endopeptidase
    Epidermal growth factor-binding protein type A
    Short name:
    EGF-BP A
    Glandular kallikrein K22
    Short name:
    mGK-22
    Nerve growth factor beta chain endopeptidase
    Tissue kallikrein 22
    Gene namesi
    Name:Klk1b22
    Synonyms:Klk-22, Klk22
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:95291. Klk1b22.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Add
    BLAST
    Propeptidei18 – 247Activation peptide2 PublicationsPRO_0000027989
    Chaini25 – 259235Kallikrein 1-related peptidase b22PRO_0000027990Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 171PROSITE-ProRule annotation
    Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
    Glycosylationi102 – 1021N-linked (GlcNAc...)Curated
    Disulfide bondi150 ↔ 217PROSITE-ProRule annotation
    Disulfide bondi182 ↔ 196PROSITE-ProRule annotation
    Disulfide bondi207 ↔ 232PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP15948.
    PaxDbiP15948.
    PRIDEiP15948.

    Expressioni

    Gene expression databases

    BgeeiP15948.
    CleanExiMM_KLK1B22.
    GenevestigatoriP15948.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000076733.

    Structurei

    3D structure databases

    ProteinModelPortaliP15948.
    SMRiP15948. Positions 25-259.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 256232Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP15948.
    KOiK01325.
    OMAiCVNINIL.
    OrthoDBiEOG75B84T.
    PhylomeDBiP15948.
    TreeFamiTF331065.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15948-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRFLILFLTL SLGGIDAAPP VQSRILGGFK CEKNSQPWQV AVYYLDEYLC    50
    GGVLLDRNWV LTAAHCYEDK YNIWLGKNKL FQDEPSAQHR LVSKSFPHPD 100
    FNMSLLQSVP TGADLSNDLM LLRLSKPADI TDVVKPIDLP TTEPKLGSTC 150
    LASGWGSINQ LIYQNPNDLQ CVSIKLHPNE VCVKAHILKV TDVMLCAGEM 200
    NGGKDTCKGD SGGPLICDGV LQGITSWGST PCGEPNAPAI YTKLIKFTSW 250
    IKDTMAKNP 259
    Length:259
    Mass (Da):28,384
    Last modified:April 1, 1990 - v1
    Checksum:iD7745794D8A87B9C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17979, M17977, M17978 Genomic DNA. Translation: AAA37682.1. Sequence problems.
    M18598 Genomic DNA. Translation: AAA39361.1.
    M18618 Genomic DNA. Translation: AAA39362.1.
    CCDSiCCDS21196.1.
    PIRiA29746.
    RefSeqiNP_034244.1. NM_010114.1.
    UniGeneiMm.5193.

    Genome annotation databases

    EnsembliENSMUST00000077528; ENSMUSP00000076733; ENSMUSG00000060177.
    GeneIDi13646.
    KEGGimmu:13646.
    UCSCiuc009goi.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17979 , M17977 , M17978 Genomic DNA. Translation: AAA37682.1 . Sequence problems.
    M18598 Genomic DNA. Translation: AAA39361.1 .
    M18618 Genomic DNA. Translation: AAA39362.1 .
    CCDSi CCDS21196.1.
    PIRi A29746.
    RefSeqi NP_034244.1. NM_010114.1.
    UniGenei Mm.5193.

    3D structure databases

    ProteinModelPortali P15948.
    SMRi P15948. Positions 25-259.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000076733.

    Protein family/group databases

    MEROPSi S01.039.

    Proteomic databases

    MaxQBi P15948.
    PaxDbi P15948.
    PRIDEi P15948.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000077528 ; ENSMUSP00000076733 ; ENSMUSG00000060177 .
    GeneIDi 13646.
    KEGGi mmu:13646.
    UCSCi uc009goi.1. mouse.

    Organism-specific databases

    CTDi 13646.
    MGIi MGI:95291. Klk1b22.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P15948.
    KOi K01325.
    OMAi CVNINIL.
    OrthoDBi EOG75B84T.
    PhylomeDBi P15948.
    TreeFami TF331065.

    Enzyme and pathway databases

    Reactomei REACT_199000. Activation of Matrix Metalloproteinases.

    Miscellaneous databases

    NextBioi 284362.
    PROi P15948.
    SOURCEi Search...

    Gene expression databases

    Bgeei P15948.
    CleanExi MM_KLK1B22.
    Genevestigatori P15948.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse glandular kallikrein genes: identification and characterization of the genes encoding the epidermal growth factor binding proteins."
      Drinkwater C.C., Evans B.A., Richards R.I.
      Biochemistry 26:6750-6756(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
      Tissue: Salivary gland.
    2. "Beta-NGF-endopeptidase: structure and activity of a kallikrein encoded by the gene mGK-22."
      Fahnestock M., Woo J.E., Lopez G.A., Snow J., Walz D.A., Arici M.J., Mobley W.C.
      Biochemistry 30:3443-3450(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-54.
    3. "mGK-6-derived true tissue kallikrein is synthesized, processed, and targeted through a regulated secretory pathway in mouse pituitary AtT-20 cells."
      Peters J., Takahashi S., Tada M., Miyake Y.
      J. Biochem. 111:643-648(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-41.
      Tissue: Submandibular gland.
    4. "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
      Evans B.A., Drinkwater C.C., Richards R.I.
      J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-54 AND 70-120.

    Entry informationi

    Entry nameiK1B22_MOUSE
    AccessioniPrimary (citable) accession number: P15948
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3