SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P15948

- K1B22_MOUSE

UniProt

P15948 - K1B22_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Kallikrein 1-related peptidase b22
Gene
Klk1b22, Klk-22, Klk22
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei118 – 1181Charge relay system
Active sitei211 – 2111Charge relay system

GO - Molecular functioni

  1. peptidase activity Source: MGI
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. zymogen activation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.

Protein family/group databases

MEROPSiS01.039.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b22 (EC:3.4.21.35)
Alternative name(s):
Beta-NGF-endopeptidase
Epidermal growth factor-binding protein type A
Short name:
EGF-BP A
Glandular kallikrein K22
Short name:
mGK-22
Nerve growth factor beta chain endopeptidase
Tissue kallikrein 22
Gene namesi
Name:Klk1b22
Synonyms:Klk-22, Klk22
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:95291. Klk1b22.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717
Add
BLAST
Propeptidei18 – 247Activation peptide
PRO_0000027989
Chaini25 – 259235Kallikrein 1-related peptidase b22
PRO_0000027990Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 171 By similarity
Disulfide bondi50 ↔ 66 By similarity
Glycosylationi102 – 1021N-linked (GlcNAc...) Inferred
Disulfide bondi150 ↔ 217 By similarity
Disulfide bondi182 ↔ 196 By similarity
Disulfide bondi207 ↔ 232 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP15948.
PaxDbiP15948.
PRIDEiP15948.

Expressioni

Gene expression databases

BgeeiP15948.
CleanExiMM_KLK1B22.
GenevestigatoriP15948.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076733.

Structurei

3D structure databases

ProteinModelPortaliP15948.
SMRiP15948. Positions 25-259.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 256232Peptidase S1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15948.
KOiK01325.
OMAiCVNINIL.
OrthoDBiEOG75B84T.
PhylomeDBiP15948.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15948-1 [UniParc]FASTAAdd to Basket

« Hide

MRFLILFLTL SLGGIDAAPP VQSRILGGFK CEKNSQPWQV AVYYLDEYLC    50
GGVLLDRNWV LTAAHCYEDK YNIWLGKNKL FQDEPSAQHR LVSKSFPHPD 100
FNMSLLQSVP TGADLSNDLM LLRLSKPADI TDVVKPIDLP TTEPKLGSTC 150
LASGWGSINQ LIYQNPNDLQ CVSIKLHPNE VCVKAHILKV TDVMLCAGEM 200
NGGKDTCKGD SGGPLICDGV LQGITSWGST PCGEPNAPAI YTKLIKFTSW 250
IKDTMAKNP 259
Length:259
Mass (Da):28,384
Last modified:April 1, 1990 - v1
Checksum:iD7745794D8A87B9C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17979, M17977, M17978 Genomic DNA. Translation: AAA37682.1. Sequence problems.
M18598 Genomic DNA. Translation: AAA39361.1.
M18618 Genomic DNA. Translation: AAA39362.1.
CCDSiCCDS21196.1.
PIRiA29746.
RefSeqiNP_034244.1. NM_010114.1.
UniGeneiMm.5193.

Genome annotation databases

EnsembliENSMUST00000077528; ENSMUSP00000076733; ENSMUSG00000060177.
GeneIDi13646.
KEGGimmu:13646.
UCSCiuc009goi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17979 , M17977 , M17978 Genomic DNA. Translation: AAA37682.1 . Sequence problems.
M18598 Genomic DNA. Translation: AAA39361.1 .
M18618 Genomic DNA. Translation: AAA39362.1 .
CCDSi CCDS21196.1.
PIRi A29746.
RefSeqi NP_034244.1. NM_010114.1.
UniGenei Mm.5193.

3D structure databases

ProteinModelPortali P15948.
SMRi P15948. Positions 25-259.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000076733.

Protein family/group databases

MEROPSi S01.039.

Proteomic databases

MaxQBi P15948.
PaxDbi P15948.
PRIDEi P15948.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000077528 ; ENSMUSP00000076733 ; ENSMUSG00000060177 .
GeneIDi 13646.
KEGGi mmu:13646.
UCSCi uc009goi.1. mouse.

Organism-specific databases

CTDi 13646.
MGIi MGI:95291. Klk1b22.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P15948.
KOi K01325.
OMAi CVNINIL.
OrthoDBi EOG75B84T.
PhylomeDBi P15948.
TreeFami TF331065.

Enzyme and pathway databases

Reactomei REACT_199000. Activation of Matrix Metalloproteinases.

Miscellaneous databases

NextBioi 284362.
PROi P15948.
SOURCEi Search...

Gene expression databases

Bgeei P15948.
CleanExi MM_KLK1B22.
Genevestigatori P15948.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mouse glandular kallikrein genes: identification and characterization of the genes encoding the epidermal growth factor binding proteins."
    Drinkwater C.C., Evans B.A., Richards R.I.
    Biochemistry 26:6750-6756(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
    Tissue: Salivary gland.
  2. "Beta-NGF-endopeptidase: structure and activity of a kallikrein encoded by the gene mGK-22."
    Fahnestock M., Woo J.E., Lopez G.A., Snow J., Walz D.A., Arici M.J., Mobley W.C.
    Biochemistry 30:3443-3450(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-54.
  3. "mGK-6-derived true tissue kallikrein is synthesized, processed, and targeted through a regulated secretory pathway in mouse pituitary AtT-20 cells."
    Peters J., Takahashi S., Tada M., Miyake Y.
    J. Biochem. 111:643-648(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-41.
    Tissue: Submandibular gland.
  4. "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
    Evans B.A., Drinkwater C.C., Richards R.I.
    J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-54 AND 70-120.

Entry informationi

Entry nameiK1B22_MOUSE
AccessioniPrimary (citable) accession number: P15948
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 3, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi