P15948 (K1B22_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kallikrein 1-related peptidase b22
EC=3.4.21.35

Alternative name(s):
Beta-NGF-endopeptidase
Epidermal growth factor-binding protein type A
Short name=EGF-BP A
Glandular kallikrein K22
Short name=mGK-22
Nerve growth factor beta chain endopeptidase
Tissue kallikrein 22

Gene names

Name:	Klk1b22
Synonyms:	Klk-22, Klk22

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW zymogen activation Inferred from direct assay PubMed 9507064. Source: MGI
Molecular_function	peptidase activity Inferred from direct assay PubMed 9507064. Source: MGI serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17
	Propeptide	18 – 24	7	Activation peptide		PRO_0000027989
	Chain	25 – 259	235	Kallikrein 1-related peptidase b22		PRO_0000027990
Regions
	Domain	25 – 256	232	Peptidase S1
Sites
	Active site	65	1	Charge relay system
	Active site	118	1	Charge relay system
	Active site	211	1	Charge relay system
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Probable
	Disulfide bond	31 ↔ 171		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	150 ↔ 217		By similarity
	Disulfide bond	182 ↔ 196		By similarity
	Disulfide bond	207 ↔ 232		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15948 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: D7745794D8A87B9C	FASTA	259	28,384
	10 20 30 40 50 60 MRFLILFLTL SLGGIDAAPP VQSRILGGFK CEKNSQPWQV AVYYLDEYLC GGVLLDRNWV 70 80 90 100 110 120 LTAAHCYEDK YNIWLGKNKL FQDEPSAQHR LVSKSFPHPD FNMSLLQSVP TGADLSNDLM 130 140 150 160 170 180 LLRLSKPADI TDVVKPIDLP TTEPKLGSTC LASGWGSINQ LIYQNPNDLQ CVSIKLHPNE 190 200 210 220 230 240 VCVKAHILKV TDVMLCAGEM NGGKDTCKGD SGGPLICDGV LQGITSWGST PCGEPNAPAI 250 YTKLIKFTSW IKDTMAKNP

References

[1]	"Mouse glandular kallikrein genes: identification and characterization of the genes encoding the epidermal growth factor binding proteins." Drinkwater C.C., Evans B.A., Richards R.I. Biochemistry 26:6750-6756(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Salivary gland.
[2]	"Beta-NGF-endopeptidase: structure and activity of a kallikrein encoded by the gene mGK-22." Fahnestock M., Woo J.E., Lopez G.A., Snow J., Walz D.A., Arici M.J., Mobley W.C. Biochemistry 30:3443-3450(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-54.
[3]	"mGK-6-derived true tissue kallikrein is synthesized, processed, and targeted through a regulated secretory pathway in mouse pituitary AtT-20 cells." Peters J., Takahashi S., Tada M., Miyake Y. J. Biochem. 111:643-648(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-41. Tissue: Submandibular gland.
[4]	"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus." Evans B.A., Drinkwater C.C., Richards R.I. J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-54 AND 70-120.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M17979, M17977, M17978 Genomic DNA. Translation: AAA37682.1. Sequence problems. M18598 Genomic DNA. Translation: AAA39361.1. M18618 Genomic DNA. Translation: AAA39362.1.
IPI	IPI00136038.
PIR	A29746.
RefSeq	NP_034244.1. NM_010114.1.
UniGene	Mm.5193.
3D structure databases
ProteinModelPortal	P15948.
SMR	P15948. Positions 25-259.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000076733.
Protein family/group databases
MEROPS	S01.039.
Proteomic databases
PaxDb	P15948.
PRIDE	P15948.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000077528; ENSMUSP00000076733; ENSMUSG00000060177.
GeneID	13646.
KEGG	mmu:13646.
Organism-specific databases
CTD	13646.
MGI	MGI:95291. Klk1b22.
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P15948.
KO	K01325.
OMA	CAGEMNG.
OrthoDB	EOG4DZ1VW.
Gene expression databases
ArrayExpress	P15948.
Bgee	P15948.
CleanEx	MM_KLK1B22.
Genevestigator	P15948.
GermOnline	ENSMUSG00000060177. Mus musculus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	284362.
SOURCE	Search...

Entry information

Entry name

K1B22_MOUSE

Accession

Primary (citable) accession number: P15948

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program