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Protein

Kallikrein 1-related peptidase b22

Gene

Klk1b22

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei118 – 1181Charge relay system
Active sitei211 – 2111Charge relay system

GO - Molecular functioni

  1. peptidase activity Source: MGI
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. zymogen activation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_331346. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
REACT_332323. Activation of Matrix Metalloproteinases.

Protein family/group databases

MEROPSiS01.039.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b22 (EC:3.4.21.35)
Alternative name(s):
Beta-NGF-endopeptidase
Epidermal growth factor-binding protein type A
Short name:
EGF-BP A
Glandular kallikrein K22
Short name:
mGK-22
Nerve growth factor beta chain endopeptidase
Tissue kallikrein 22
Gene namesi
Name:Klk1b22
Synonyms:Klk-22, Klk22
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:95291. Klk1b22.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Add
BLAST
Propeptidei18 – 247Activation peptide2 PublicationsPRO_0000027989
Chaini25 – 259235Kallikrein 1-related peptidase b22PRO_0000027990Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 171PROSITE-ProRule annotation
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Glycosylationi102 – 1021N-linked (GlcNAc...)Curated
Disulfide bondi150 ↔ 217PROSITE-ProRule annotation
Disulfide bondi182 ↔ 196PROSITE-ProRule annotation
Disulfide bondi207 ↔ 232PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP15948.
PaxDbiP15948.
PRIDEiP15948.

Expressioni

Gene expression databases

BgeeiP15948.
CleanExiMM_KLK1B22.
ExpressionAtlasiP15948. baseline and differential.
GenevestigatoriP15948.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076733.

Structurei

3D structure databases

ProteinModelPortaliP15948.
SMRiP15948. Positions 25-259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 256232Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15948.
KOiK01325.
OMAiCIEAYNE.
OrthoDBiEOG75B84T.
PhylomeDBiP15948.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFLILFLTL SLGGIDAAPP VQSRILGGFK CEKNSQPWQV AVYYLDEYLC
60 70 80 90 100
GGVLLDRNWV LTAAHCYEDK YNIWLGKNKL FQDEPSAQHR LVSKSFPHPD
110 120 130 140 150
FNMSLLQSVP TGADLSNDLM LLRLSKPADI TDVVKPIDLP TTEPKLGSTC
160 170 180 190 200
LASGWGSINQ LIYQNPNDLQ CVSIKLHPNE VCVKAHILKV TDVMLCAGEM
210 220 230 240 250
NGGKDTCKGD SGGPLICDGV LQGITSWGST PCGEPNAPAI YTKLIKFTSW

IKDTMAKNP
Length:259
Mass (Da):28,384
Last modified:April 1, 1990 - v1
Checksum:iD7745794D8A87B9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17979, M17977, M17978 Genomic DNA. Translation: AAA37682.1. Sequence problems.
M18598 Genomic DNA. Translation: AAA39361.1.
M18618 Genomic DNA. Translation: AAA39362.1.
CCDSiCCDS21196.1.
PIRiA29746.
RefSeqiNP_034244.1. NM_010114.1.
UniGeneiMm.5193.

Genome annotation databases

EnsembliENSMUST00000077528; ENSMUSP00000076733; ENSMUSG00000060177.
GeneIDi13646.
KEGGimmu:13646.
UCSCiuc009goi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17979, M17977, M17978 Genomic DNA. Translation: AAA37682.1. Sequence problems.
M18598 Genomic DNA. Translation: AAA39361.1.
M18618 Genomic DNA. Translation: AAA39362.1.
CCDSiCCDS21196.1.
PIRiA29746.
RefSeqiNP_034244.1. NM_010114.1.
UniGeneiMm.5193.

3D structure databases

ProteinModelPortaliP15948.
SMRiP15948. Positions 25-259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076733.

Protein family/group databases

MEROPSiS01.039.

Proteomic databases

MaxQBiP15948.
PaxDbiP15948.
PRIDEiP15948.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077528; ENSMUSP00000076733; ENSMUSG00000060177.
GeneIDi13646.
KEGGimmu:13646.
UCSCiuc009goi.1. mouse.

Organism-specific databases

CTDi13646.
MGIiMGI:95291. Klk1b22.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15948.
KOiK01325.
OMAiCIEAYNE.
OrthoDBiEOG75B84T.
PhylomeDBiP15948.
TreeFamiTF331065.

Enzyme and pathway databases

ReactomeiREACT_331346. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
REACT_332323. Activation of Matrix Metalloproteinases.

Miscellaneous databases

NextBioi284362.
PROiP15948.
SOURCEiSearch...

Gene expression databases

BgeeiP15948.
CleanExiMM_KLK1B22.
ExpressionAtlasiP15948. baseline and differential.
GenevestigatoriP15948.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mouse glandular kallikrein genes: identification and characterization of the genes encoding the epidermal growth factor binding proteins."
    Drinkwater C.C., Evans B.A., Richards R.I.
    Biochemistry 26:6750-6756(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
    Tissue: Salivary gland.
  2. "Beta-NGF-endopeptidase: structure and activity of a kallikrein encoded by the gene mGK-22."
    Fahnestock M., Woo J.E., Lopez G.A., Snow J., Walz D.A., Arici M.J., Mobley W.C.
    Biochemistry 30:3443-3450(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-54.
  3. "mGK-6-derived true tissue kallikrein is synthesized, processed, and targeted through a regulated secretory pathway in mouse pituitary AtT-20 cells."
    Peters J., Takahashi S., Tada M., Miyake Y.
    J. Biochem. 111:643-648(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-41.
    Tissue: Submandibular gland.
  4. "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
    Evans B.A., Drinkwater C.C., Richards R.I.
    J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-54 AND 70-120.

Entry informationi

Entry nameiK1B22_MOUSE
AccessioniPrimary (citable) accession number: P15948
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 1, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.