Reviewed,
UniProtKB/Swiss-Prot P15948 (K1B22_MOUSE)
Last modified
May 5, 2009.
Version 79.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Kallikrein 1-related peptidase b22 EC=3.4.21.35 Alternative name(s): Glandular kallikrein K22 Short name=mGK-22 Tissue kallikrein 22 Epidermal growth factor-binding protein type A Short name=EGF-BP A Nerve growth factor beta chain endopeptidase Beta-NGF-endopeptidase | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 259 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. |
| Catalytic activity | Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. |
| Sequence similarities | Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain. |
Ontologies
| Keywords | |
|---|---|
| Domain | Signal |
| Molecular function | Hydrolase Protease Serine protease |
| PTM | Disulfide bond Glycoprotein Zymogen |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | proteolysis Inferred from electronic annotation. Source: InterPro |
| Molecular function | serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 17 | 17 | |||||||||
| Propeptide | 18 – 24 | 7 | Activation peptide Ref.2 Ref.3 | PRO_0000027989 | |||||||
| Chain | 25 – 259 | 235 | Kallikrein 1-related peptidase b22 | PRO_0000027990 | |||||||
Regions | |||||||||||
| Domain | 25 – 256 | 232 | Peptidase S1 | ||||||||
Sites | |||||||||||
| Active site | 65 | 1 | Charge relay system | ||||||||
| Active site | 118 | 1 | Charge relay system | ||||||||
| Active site | 211 | 1 | Charge relay system | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 102 | 1 | N-linked (GlcNAc...) Probable | ||||||||
| Disulfide bond | 31 ↔ 171 | By similarity | |||||||||
| Disulfide bond | 50 ↔ 66 | By similarity | |||||||||
| Disulfide bond | 150 ↔ 217 | By similarity | |||||||||
| Disulfide bond | 182 ↔ 196 | By similarity | |||||||||
| Disulfide bond | 207 ↔ 232 | By similarity | |||||||||
Sequences
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References
| [1] | "Mouse glandular kallikrein genes: identification and characterization of the genes encoding the epidermal growth factor binding proteins." Drinkwater C.C., Evans B.A., Richards R.I. Biochemistry 26:6750-6756(1987) [PubMed: 3322387] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Salivary gland. |
| [2] | "Beta-NGF-endopeptidase: structure and activity of a kallikrein encoded by the gene mGK-22." Fahnestock M., Woo J.E., Lopez G.A., Snow J., Walz D.A., Arici M.J., Mobley W.C. Biochemistry 30:3443-3450(1991) [PubMed: 2012805] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-54. |
| [3] | "mGK-6-derived true tissue kallikrein is synthesized, processed, and targeted through a regulated secretory pathway in mouse pituitary AtT-20 cells." Peters J., Takahashi S., Tada M., Miyake Y. J. Biochem. 111:643-648(1992) [PubMed: 1639762] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-41. Tissue: Submandibular gland. |
| [4] | "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus." Evans B.A., Drinkwater C.C., Richards R.I. J. Biol. Chem. 262:8027-8034(1987) [PubMed: 3036794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-54 AND 70-120. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M17979, M17977, M17978 Genomic DNA. Translation: AAA37682.1. Sequence problems. M18598 Genomic DNA. Translation: AAA39361.1. M18618 Genomic DNA. Translation: AAA39362.1. | |
| IPI | IPI00136038. |
| PIR | A29746. |
| RefSeq | NP_034244.1. |
| UniGene | Mm.5193 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1SGF based on UniProtKB P00757. |
| SMR | P15948. Positions 25-258. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | S01.039. |
Proteomic databases | |
| PRIDE | P15948. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000060177. Mus musculus. [Contig view] |
| GeneID | 13646. |
| KEGG | mmu:13646. |
Organism-specific databases | |
| MGI | MGI:95291. Klk1b22. |
Phylogenomic databases | |
| HOVERGEN | P15948. |
| OMA | P15948. CAGEMNG. |
Enzyme and pathway databases | |
| BRENDA | 3.4.21.35. 244. |
Gene expression databases | |
| CleanEx | MM_KLK1B22. |
| GermOnline | ENSMUSG00000060177. Mus musculus. |
Family and domain databases | |
| InterPro | IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view] |
| Pfam | PF00089. Trypsin. 1 hit. [Graphical view] |
| PRINTS | PR00722. CHYMOTRYPSIN. |
| SMART | SM00020. Tryp_SPc. 1 hit. [Graphical view] |
| PROSITE | PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 284362. |
| SOURCE | Search... |
Entry information
| Entry name | K1B22_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P15948 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


