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P15947

- KLK1_MOUSE

UniProt

P15947 - KLK1_MOUSE

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Protein
Kallikrein-1
Gene
Klk1, Klk-6, Klk6
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei120 – 1201Charge relay system
Active sitei213 – 2131Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.

    Protein family/group databases

    MEROPSiS01.167.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kallikrein-1 (EC:3.4.21.35)
    Alternative name(s):
    Glandular kallikrein K1
    KAL-B
    Renal kallikrein
    Tissue kallikrein-6
    Short name:
    mGK-6
    Gene namesi
    Name:Klk1
    Synonyms:Klk-6, Klk6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:102850. Klk1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818 Inferred
    Add
    BLAST
    Propeptidei19 – 246Activation peptide
    PRO_0000027975
    Chaini25 – 261237Kallikrein-1
    PRO_0000027976Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 173 By similarity
    Disulfide bondi50 ↔ 66 By similarity
    Glycosylationi102 – 1021N-linked (GlcNAc...) Inferred
    Disulfide bondi152 ↔ 219 By similarity
    Disulfide bondi184 ↔ 198 By similarity
    Disulfide bondi209 ↔ 234 By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP15947.
    PaxDbiP15947.
    PRIDEiP15947.

    Expressioni

    Gene expression databases

    BgeeiP15947.
    CleanExiMM_KLK1.
    GenevestigatoriP15947.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000074659.

    Structurei

    3D structure databases

    ProteinModelPortaliP15947.
    SMRiP15947. Positions 25-260.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 258234Peptidase S1
    Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00750000117405.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP15947.
    KOiK01325.
    OMAiFNTWIRE.
    OrthoDBiEOG75B84T.
    PhylomeDBiP15947.
    TreeFamiTF331065.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15947-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRFLILFLAL SLGGIDAAPP VQSRIVGGFN CEKNSQPWQV AVYRFTKYQC    50
    GGILLNANWV LTAAHCHNDK YQVWLGKNNF LEDEPSAQHR LVSKAIPHPD 100
    FNMSLLNEHT PQPEDDYSND LMLLRLKKPA DITDVVKPID LPTEEPKLGS 150
    TCLASGWGSI TPVKYEYPDE LQCVNLKLLP NEDCAKAHIE KVTDDMLCAG 200
    DMDGGKDTCA GDSGGPLICD GVLQGITSWG PSPCGKPNVP GIYTRVLNFN 250
    TWIRETMAEN D 261
    Length:261
    Mass (Da):28,775
    Last modified:December 5, 2001 - v3
    Checksum:i7850DDFDBFFB94B8
    GO

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 571A → V in AAG11389. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13500, M13498, M13499 Genomic DNA. Translation: AAG11389.1.
    D10464 mRNA. Translation: BAA01257.1.
    AK002278 mRNA. Translation: BAB21982.1.
    BC010754 mRNA. Translation: AAH10754.1.
    BC027736 mRNA. Translation: AAH27736.1.
    BC053697 mRNA. Translation: AAH53697.1.
    CCDSiCCDS21202.1.
    PIRiA25606.
    RefSeqiNP_034769.4. NM_010639.7.
    UniGeneiMm.142722.

    Genome annotation databases

    EnsembliENSMUST00000075162; ENSMUSP00000074659; ENSMUSG00000063903.
    GeneIDi16612.
    KEGGimmu:16612.
    UCSCiuc009goo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13500 , M13498 , M13499 Genomic DNA. Translation: AAG11389.1 .
    D10464 mRNA. Translation: BAA01257.1 .
    AK002278 mRNA. Translation: BAB21982.1 .
    BC010754 mRNA. Translation: AAH10754.1 .
    BC027736 mRNA. Translation: AAH27736.1 .
    BC053697 mRNA. Translation: AAH53697.1 .
    CCDSi CCDS21202.1.
    PIRi A25606.
    RefSeqi NP_034769.4. NM_010639.7.
    UniGenei Mm.142722.

    3D structure databases

    ProteinModelPortali P15947.
    SMRi P15947. Positions 25-260.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000074659.

    Protein family/group databases

    MEROPSi S01.167.

    Proteomic databases

    MaxQBi P15947.
    PaxDbi P15947.
    PRIDEi P15947.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000075162 ; ENSMUSP00000074659 ; ENSMUSG00000063903 .
    GeneIDi 16612.
    KEGGi mmu:16612.
    UCSCi uc009goo.1. mouse.

    Organism-specific databases

    CTDi 3816.
    MGIi MGI:102850. Klk1.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00750000117405.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P15947.
    KOi K01325.
    OMAi FNTWIRE.
    OrthoDBi EOG75B84T.
    PhylomeDBi P15947.
    TreeFami TF331065.

    Enzyme and pathway databases

    Reactomei REACT_199000. Activation of Matrix Metalloproteinases.

    Miscellaneous databases

    NextBioi 290197.
    PROi P15947.
    SOURCEi Search...

    Gene expression databases

    Bgeei P15947.
    CleanExi MM_KLK1.
    Genevestigatori P15947.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Mouse glandular kallikrein genes. Identification, structure, and expression of the renal kallikrein gene."
      van Leeuwen B.H., Evans B.A., Tregear G.W., Richards R.I.
      J. Biol. Chem. 261:5529-5535(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of a tissue kallikrein gene, mGK-6, expressed in a mouse neuroendocrine cell line."
      Tada M., Peters J., Takahashi S., Inoue H., Miyake Y.
      Submitted (JUN-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Kidney.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon, Kidney and Salivary gland.
    5. "mGK-6-derived true tissue kallikrein is synthesized, processed, and targeted through a regulated secretory pathway in mouse pituitary AtT-20 cells."
      Peters J., Takahashi S., Tada M., Miyake Y.
      J. Biochem. 111:643-648(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-44.
      Tissue: Submandibular gland.
    6. "A cytocidal tissue kallikrein isolated from mouse submandibular glands."
      Murakami K., Ikigai H., Nagumo N., Tomita M., Shimamura T.
      FEBS Lett. 257:400-402(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 165-174.
      Tissue: Submandibular gland.

    Entry informationi

    Entry nameiKLK1_MOUSE
    AccessioniPrimary (citable) accession number: P15947
    Secondary accession number(s): Q61855
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: December 5, 2001
    Last modified: September 3, 2014
    This is version 136 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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