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Protein

Kallikrein-1

Gene

Klk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay system1
Active sitei120Charge relay system1
Active sitei213Charge relay system1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.B10. 3474.
ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiS01.167.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein-1 (EC:3.4.21.35)
Alternative name(s):
Glandular kallikrein K1
KAL-B
Renal kallikrein
Tissue kallikrein-6
Short name:
mGK-6
Gene namesi
Name:Klk1
Synonyms:Klk-6, Klk6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:102850. Klk1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18CuratedAdd BLAST18
PropeptideiPRO_000002797519 – 24Activation peptide1 Publication6
ChainiPRO_000002797625 – 261Kallikrein-1Add BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 173PROSITE-ProRule annotation
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Glycosylationi102N-linked (GlcNAc...)Curated1
Disulfide bondi152 ↔ 219PROSITE-ProRule annotation
Disulfide bondi184 ↔ 198PROSITE-ProRule annotation
Disulfide bondi209 ↔ 234PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP15947.
PaxDbiP15947.
PeptideAtlasiP15947.
PRIDEiP15947.

Expressioni

Gene expression databases

BgeeiENSMUSG00000063903.
CleanExiMM_KLK1.
ExpressionAtlasiP15947. baseline and differential.
GenevisibleiP15947. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074659.

Structurei

3D structure databases

ProteinModelPortaliP15947.
SMRiP15947.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 258Peptidase S1PROSITE-ProRule annotationAdd BLAST234

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15947.
KOiK01325.
OMAiFNTWIRE.
OrthoDBiEOG091G0DF7.
PhylomeDBiP15947.
TreeFamiTF331065.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFLILFLAL SLGGIDAAPP VQSRIVGGFN CEKNSQPWQV AVYRFTKYQC
60 70 80 90 100
GGILLNANWV LTAAHCHNDK YQVWLGKNNF LEDEPSAQHR LVSKAIPHPD
110 120 130 140 150
FNMSLLNEHT PQPEDDYSND LMLLRLKKPA DITDVVKPID LPTEEPKLGS
160 170 180 190 200
TCLASGWGSI TPVKYEYPDE LQCVNLKLLP NEDCAKAHIE KVTDDMLCAG
210 220 230 240 250
DMDGGKDTCA GDSGGPLICD GVLQGITSWG PSPCGKPNVP GIYTRVLNFN
260
TWIRETMAEN D
Length:261
Mass (Da):28,775
Last modified:December 5, 2001 - v3
Checksum:i7850DDFDBFFB94B8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57A → V in AAG11389 (PubMed:3007510).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13500, M13498, M13499 Genomic DNA. Translation: AAG11389.1.
D10464 mRNA. Translation: BAA01257.1.
AK002278 mRNA. Translation: BAB21982.1.
BC010754 mRNA. Translation: AAH10754.1.
BC027736 mRNA. Translation: AAH27736.1.
BC053697 mRNA. Translation: AAH53697.1.
CCDSiCCDS21202.1.
PIRiA25606.
RefSeqiNP_001307260.1. NM_001320331.1.
NP_001307261.1. NM_001320332.1.
NP_034769.4. NM_010639.8.
UniGeneiMm.142722.

Genome annotation databases

EnsembliENSMUST00000075162; ENSMUSP00000074659; ENSMUSG00000063903.
GeneIDi16612.
KEGGimmu:16612.
UCSCiuc009goo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13500, M13498, M13499 Genomic DNA. Translation: AAG11389.1.
D10464 mRNA. Translation: BAA01257.1.
AK002278 mRNA. Translation: BAB21982.1.
BC010754 mRNA. Translation: AAH10754.1.
BC027736 mRNA. Translation: AAH27736.1.
BC053697 mRNA. Translation: AAH53697.1.
CCDSiCCDS21202.1.
PIRiA25606.
RefSeqiNP_001307260.1. NM_001320331.1.
NP_001307261.1. NM_001320332.1.
NP_034769.4. NM_010639.8.
UniGeneiMm.142722.

3D structure databases

ProteinModelPortaliP15947.
SMRiP15947.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074659.

Protein family/group databases

MEROPSiS01.167.

Proteomic databases

MaxQBiP15947.
PaxDbiP15947.
PeptideAtlasiP15947.
PRIDEiP15947.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075162; ENSMUSP00000074659; ENSMUSG00000063903.
GeneIDi16612.
KEGGimmu:16612.
UCSCiuc009goo.1. mouse.

Organism-specific databases

CTDi3816.
MGIiMGI:102850. Klk1.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15947.
KOiK01325.
OMAiFNTWIRE.
OrthoDBiEOG091G0DF7.
PhylomeDBiP15947.
TreeFamiTF331065.

Enzyme and pathway databases

BRENDAi3.4.21.B10. 3474.
ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

PROiP15947.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063903.
CleanExiMM_KLK1.
ExpressionAtlasiP15947. baseline and differential.
GenevisibleiP15947. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKLK1_MOUSE
AccessioniPrimary (citable) accession number: P15947
Secondary accession number(s): Q61855
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 5, 2001
Last modified: November 2, 2016
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.