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P15947 (KLK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein-1
EC=3.4.21.35
Alternative name(s):
Glandular kallikrein K1
KAL-B
Renal kallikrein
Tissue kallikrein-6
Short name=mGK-6
Gene names
Name:Klk1
Synonyms:Klk-6, Klk6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Probable
Propeptide19 – 246Activation peptide
PRO_0000027975
Chain25 – 261237Kallikrein-1
PRO_0000027976

Regions

Domain25 – 258234Peptidase S1

Sites

Active site651Charge relay system
Active site1201Charge relay system
Active site2131Charge relay system

Amino acid modifications

Glycosylation1021N-linked (GlcNAc...) Probable
Disulfide bond31 ↔ 173 By similarity
Disulfide bond50 ↔ 66 By similarity
Disulfide bond152 ↔ 219 By similarity
Disulfide bond184 ↔ 198 By similarity
Disulfide bond209 ↔ 234 By similarity

Experimental info

Sequence conflict571A → V in AAG11389. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P15947 [UniParc].

Last modified December 5, 2001. Version 3.
Checksum: 7850DDFDBFFB94B8

FASTA26128,775
        10         20         30         40         50         60 
MRFLILFLAL SLGGIDAAPP VQSRIVGGFN CEKNSQPWQV AVYRFTKYQC GGILLNANWV 

        70         80         90        100        110        120 
LTAAHCHNDK YQVWLGKNNF LEDEPSAQHR LVSKAIPHPD FNMSLLNEHT PQPEDDYSND 

       130        140        150        160        170        180 
LMLLRLKKPA DITDVVKPID LPTEEPKLGS TCLASGWGSI TPVKYEYPDE LQCVNLKLLP 

       190        200        210        220        230        240 
NEDCAKAHIE KVTDDMLCAG DMDGGKDTCA GDSGGPLICD GVLQGITSWG PSPCGKPNVP 

       250        260 
GIYTRVLNFN TWIRETMAEN D

« Hide

References

« Hide 'large scale' references
[1]"Mouse glandular kallikrein genes. Identification, structure, and expression of the renal kallikrein gene."
van Leeuwen B.H., Evans B.A., Tregear G.W., Richards R.I.
J. Biol. Chem. 261:5529-5535(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification of a tissue kallikrein gene, mGK-6, expressed in a mouse neuroendocrine cell line."
Tada M., Peters J., Takahashi S., Inoue H., Miyake Y.
Submitted (JUN-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon, Kidney and Salivary gland.
[5]"mGK-6-derived true tissue kallikrein is synthesized, processed, and targeted through a regulated secretory pathway in mouse pituitary AtT-20 cells."
Peters J., Takahashi S., Tada M., Miyake Y.
J. Biochem. 111:643-648(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-44.
Tissue: Submandibular gland.
[6]"A cytocidal tissue kallikrein isolated from mouse submandibular glands."
Murakami K., Ikigai H., Nagumo N., Tomita M., Shimamura T.
FEBS Lett. 257:400-402(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 165-174.
Tissue: Submandibular gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M13500, M13498, M13499 Genomic DNA. Translation: AAG11389.1.
D10464 mRNA. Translation: BAA01257.1.
AK002278 mRNA. Translation: BAB21982.1.
BC010754 mRNA. Translation: AAH10754.1.
BC027736 mRNA. Translation: AAH27736.1.
BC053697 mRNA. Translation: AAH53697.1.
IPIIPI00323869.
PIRA25606.
RefSeqNP_034769.4. NM_010639.7.
UniGeneMm.142722.

3D structure databases

ProteinModelPortalP15947.
SMRP15947. Positions 25-260.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000074659.

Protein family/group databases

MEROPSS01.167.

Proteomic databases

PaxDbP15947.
PRIDEP15947.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000075162; ENSMUSP00000074659; ENSMUSG00000063903.
GeneID16612.
KEGGmmu:16612.
UCSCuc009gon.1. mouse.

Organism-specific databases

CTD3816.
MGIMGI:102850. Klk1.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00690000101721.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP15947.
KOK01325.
OMAFNTWIRE.
OrthoDBEOG4DZ1VW.

Gene expression databases

BgeeP15947.
CleanExMM_KLK1.
GenevestigatorP15947.
GermOnlineENSMUSG00000063903. Mus musculus.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. Pept_Ser_Cys. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio290197.
SOURCESearch...

Entry information

Entry nameKLK1_MOUSE
AccessionPrimary (citable) accession number: P15947
Secondary accession number(s): Q61855
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 5, 2001
Last modified: April 3, 2013
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents