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Protein

Amyloid-like protein 2

Gene

Aplp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein. Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei322 – 3232Reactive bondBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI02.016.

Names & Taxonomyi

Protein namesi
Recommended name:
Amyloid-like protein 2
Short name:
APLP-2
Alternative name(s):
Sperm membrane protein YWK-II
Gene namesi
Name:Aplp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2128. Aplp2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 695664ExtracellularSequence analysisAdd
BLAST
Transmembranei696 – 71823HelicalSequence analysisAdd
BLAST
Topological domaini719 – 76547CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 765734Amyloid-like protein 2PRO_0000000209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 2161PhosphoserineCombined sources
Disulfide bondi312 ↔ 362PROSITE-ProRule annotation
Disulfide bondi321 ↔ 345PROSITE-ProRule annotation
Disulfide bondi337 ↔ 358PROSITE-ProRule annotation
Modified residuei592 – 5921PhosphoserineBy similarity
Glycosylationi628 – 6281O-linked (Xyl...) (glycosaminoglycan)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP15943.
PRIDEiP15943.

PTM databases

iPTMnetiP15943.
PhosphoSiteiP15943.

Interactioni

Subunit structurei

Interacts with CPEB1. Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067835.

Structurei

3D structure databases

ProteinModelPortaliP15943.
SMRiP15943. Positions 47-139, 376-566, 737-764.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini308 – 36659BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni751 – 76515Interaction with DAB2By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi752 – 7576NPXY motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi218 – 28265Asp/Glu-rich (highly acidic)Add
BLAST
Compositional biasi218 – 22912Poly-GluAdd
BLAST

Sequence similaritiesi

Belongs to the APP family.Curated
Contains 1 BPTI/Kunitz inhibitor domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3540. Eukaryota.
ENOG410ZW2A. LUCA.
HOVERGENiHBG000051.
InParanoidiP15943.
PhylomeDBiP15943.

Family and domain databases

Gene3Di3.30.1490.140. 1 hit.
3.90.570.10. 1 hit.
4.10.410.10. 1 hit.
InterProiIPR008155. Amyloid_glyco.
IPR011178. Amyloid_glyco_Cu-bd.
IPR024329. Amyloid_glyco_E2_domain.
IPR008154. Amyloid_glyco_extra.
IPR019744. Amyloid_glyco_extracell_CS.
IPR015849. Amyloid_glyco_heparin-bd.
IPR019745. Amyloid_glyco_intracell_CS.
IPR019543. APP_amyloid_C.
IPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF10515. APP_amyloid. 1 hit.
PF12924. APP_Cu_bd. 1 hit.
PF12925. APP_E2. 1 hit.
PF02177. APP_N. 1 hit.
PF00014. Kunitz_BPTI. 1 hit.
[Graphical view]
PRINTSiPR00203. AMYLOIDA4.
PR00759. BASICPTASE.
SMARTiSM00006. A4_EXTRA. 1 hit.
SM00131. KU. 1 hit.
[Graphical view]
SUPFAMiSSF109843. SSF109843. 1 hit.
SSF56491. SSF56491. 1 hit.
SSF57362. SSF57362. 1 hit.
SSF89811. SSF89811. 1 hit.
PROSITEiPS00319. A4_EXTRA. 1 hit.
PS00320. A4_INTRA. 1 hit.
PS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P15943-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATGTAAAA ATGKLLVLLL LGLTAPAAAL AGYIEALAAN AGTGFAVAEP
60 70 80 90 100
QIAMFCGKLN MHVNIQTGKW EPDPTGTKSC LGTKEEVLQY CQEIYPELQI
110 120 130 140 150
TNVMEANQPV NIDSWCRRDK KQCRSHIVIP FKCLVGEFVS DVLLVPENCQ
160 170 180 190 200
FFHQERMEVC EKHQRWHTVV KEACLTEGMT LYSYGMLLPC GVDQFHGTEY
210 220 230 240 250
VCCPQTKVVD SDSTMSKEEE EEEEEDEEED YALDKSEFPT EADLEDFTEA
260 270 280 290 300
AADEDEDEEE EEEEEGEEVV EDRDYYYDSF KGDDYNEENP TEPSSDGTIS
310 320 330 340 350
DKEIAHDVKA VCSQEAMTGP CRAVMPRWYF DLSKGKCVRF IYGGCGGNRN
360 370 380 390 400
NFESEDYCMA VCKTMIPPTP LPTNDVDVYF ETSADDNEHA RFQKAKEQLE
410 420 430 440 450
IRHRSRMDRV KKEWEEAELQ AKNLPKAERQ TLIQHFQAMV KALEKEAASE
460 470 480 490 500
KQQLVETHLA RVEAMLNDRR RIALENYLAA LQSDPPRPHR ILQALRRYVR
510 520 530 540 550
AENKDRLHTI RHYQHVLAVD PEKAAQMKSQ VMTHLHVIEE RRNQSLSLLY
560 570 580 590 600
KVPYVAQEIQ EEIDELLQEQ RADMDQFTSS ISENPVDVRV SSEESEEIPP
610 620 630 640 650
FHPFHPFPSL SENEDTQPEL YHPMKKGSGM AEQDGGLIGA EEKVINSKNK
660 670 680 690 700
MDENMVIDET LDVKEMIFNA ERVGGLEEEP DSVGPLREDF SLSSSALIGL
710 720 730 740 750
LVIAVAIATV IVISLVMLRK RQYGTISHGI VEVHPMLTPE ERHLNKMQNH
760
GYENPTYKYL EQMQI
Length:765
Mass (Da):86,883
Last modified:October 1, 1996 - v2
Checksum:iCF51FCCCE305A0CF
GO
Isoform B (identifier: P15943-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     616-627: Missing.

Show »
Length:753
Mass (Da):85,472
Checksum:iA0343F35E7BE80FA
GO
Isoform C (identifier: P15943-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     311-365: Missing.

Show »
Length:710
Mass (Da):80,688
Checksum:iE000C3BD6726E579
GO
Isoform D (identifier: P15943-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     311-365: Missing.
     616-627: Missing.

Show »
Length:698
Mass (Da):79,278
Checksum:iDB63549BF92F0AB8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti575 – 5773DQF → EFV in AAA42352 (PubMed:1690887).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei311 – 36555Missing in isoform C and isoform D. CuratedVSP_000020Add
BLAST
Alternative sequencei616 – 62712Missing in isoform B and isoform D. CuratedVSP_000021Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77934 mRNA. Translation: CAA54906.1.
M31322 mRNA. Translation: AAA42352.1.
PIRiA35981.
S42880.
UniGeneiRn.129285.
Rn.1423.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77934 mRNA. Translation: CAA54906.1.
M31322 mRNA. Translation: AAA42352.1.
PIRiA35981.
S42880.
UniGeneiRn.129285.
Rn.1423.

3D structure databases

ProteinModelPortaliP15943.
SMRiP15943. Positions 47-139, 376-566, 737-764.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067835.

Protein family/group databases

MEROPSiI02.016.

PTM databases

iPTMnetiP15943.
PhosphoSiteiP15943.

Proteomic databases

PaxDbiP15943.
PRIDEiP15943.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi2128. Aplp2.

Phylogenomic databases

eggNOGiKOG3540. Eukaryota.
ENOG410ZW2A. LUCA.
HOVERGENiHBG000051.
InParanoidiP15943.
PhylomeDBiP15943.

Miscellaneous databases

PROiP15943.

Family and domain databases

Gene3Di3.30.1490.140. 1 hit.
3.90.570.10. 1 hit.
4.10.410.10. 1 hit.
InterProiIPR008155. Amyloid_glyco.
IPR011178. Amyloid_glyco_Cu-bd.
IPR024329. Amyloid_glyco_E2_domain.
IPR008154. Amyloid_glyco_extra.
IPR019744. Amyloid_glyco_extracell_CS.
IPR015849. Amyloid_glyco_heparin-bd.
IPR019745. Amyloid_glyco_intracell_CS.
IPR019543. APP_amyloid_C.
IPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF10515. APP_amyloid. 1 hit.
PF12924. APP_Cu_bd. 1 hit.
PF12925. APP_E2. 1 hit.
PF02177. APP_N. 1 hit.
PF00014. Kunitz_BPTI. 1 hit.
[Graphical view]
PRINTSiPR00203. AMYLOIDA4.
PR00759. BASICPTASE.
SMARTiSM00006. A4_EXTRA. 1 hit.
SM00131. KU. 1 hit.
[Graphical view]
SUPFAMiSSF109843. SSF109843. 1 hit.
SSF56491. SSF56491. 1 hit.
SSF57362. SSF57362. 1 hit.
SSF89811. SSF89811. 1 hit.
PROSITEiPS00319. A4_EXTRA. 1 hit.
PS00320. A4_INTRA. 1 hit.
PS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide and deduced amino acid sequence of rat amyloid protein precursor-like protein 2 (APLP2/APPH): two amino acids length difference to human and murine homologues."
    Sandbrink R., Masters C.L., Beyreuther K.
    Biochim. Biophys. Acta 1219:167-170(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-627.
    Strain: Wistar.
    Tissue: Brain and Heart.
  2. "Characterization of cDNA encoding a human sperm membrane protein related to A4 amyloid protein."
    Yan Y.C., Bai Y., Wang L.F., Miao S.Y., Koide S.S.
    Proc. Natl. Acad. Sci. U.S.A. 87:2405-2408(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 575-765.
    Tissue: Testis.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAPLP2_RAT
AccessioniPrimary (citable) accession number: P15943
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1996
Last modified: January 20, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.