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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

Gene

PRP16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Influences the fidelity of branchpoint recognition in yeast splicing. This is RNA-dependent ATPase which is essential for viability. It may mediate one of the many ATP-requiring steps of spliceosome assembly and that accuracy of branchpoint recognition may be coupled to ATP binding and/or hydrolysis.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi373 – 3808ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • generation of catalytic spliceosome for second transesterification step Source: SGD
  • RNA exon ligation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32049-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (EC:3.6.4.13)
Gene namesi
Name:PRP16
Ordered Locus Names:YKR086W
ORF Names:YKR406
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR086W.
SGDiS000001794. PRP16.

Subcellular locationi

  • Nucleus

  • Note: Binds to the spliceosome.

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • U2-type catalytic step 2 spliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi386 – 3861Y → D: Suppressor phenotype.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10711071Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16PRO_0000055150Add
BLAST

Proteomic databases

MaxQBiP15938.

PTM databases

iPTMnetiP15938.

Interactioni

Protein-protein interaction databases

BioGridi34217. 143 interactions.
DIPiDIP-2824N.
IntActiP15938. 20 interactions.
MINTiMINT-1699731.

Structurei

3D structure databases

ProteinModelPortaliP15938.
SMRiP15938. Positions 310-989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini360 – 526167Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini540 – 735196Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi473 – 4764DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00840000130110.
HOGENOMiHOG000175261.
InParanoidiP15938.
KOiK12815.
OMAiITSATMN.
OrthoDBiEOG7M98QW.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15938-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHSGREERI KDIFKELTSK ELTPGLLLTL QKLAQKPNTN LEQFIASCKA
60 70 80 90 100
LTKLSSNNPI IFNELLELLK NKSEEDSTGP KKIAPSINKR KKFKIQLDLD
110 120 130 140 150
DNEDELDSPV QKKPAPTRTL FKRIDKLKAK QLRQYSPTVK DPSPNSEQQT
160 170 180 190 200
QNGHAETKDY EPTRSEVVEE DREWYDNDDD YGNLVPEPLS ELPEEAKLLP
210 220 230 240 250
VIRNIDNDDA LRNTVQLYPI PLKQRMEWIP PFLSKFALEN KVPTSIIIGS
260 270 280 290 300
ISETSSQVSA LSMVNPFRNP DSEFSANAKR GSKLVALRRI NMEHIQQSRD
310 320 330 340 350
NTTVLNTAMG EVLGLENNNK AKDKSNQKIC DDTALFTPSK DDIKHTKEQL
360 370 380 390 400
PVFRCRSQLL SLIRENQVVV IIGETGSGKT TQLAQYLYEE GYANDRGKSI
410 420 430 440 450
VVTQPRRVAA ISVAKRVAME MQVPLGKEVG YSIRFEDVTD SECTKLKFVT
460 470 480 490 500
DGILLRETLL DDTLDKYSCV IIDEAHERSL NTDILLGFFK ILLARRRDLK
510 520 530 540 550
LIITSATMNA KKFSAFFGNA PQFTIPGRTF PVQTIYTSNP VQDYVEAAVS
560 570 580 590 600
QAVKIHLAND CSSGDILIFM TGQEDIETTF DTLQEKFLQV YSKKFGTANF
610 620 630 640 650
EEINDIEILP IYSALPADLQ FKIFQDLHGT KRKIIIATNI AETSLTIKGI
660 670 680 690 700
RYVIDCGYSK LKVYNPKIGL DSLVITPISK ANADQRSGRA GRTAPGTAYR
710 720 730 740 750
LYTEDTFKED MYLQTIPEIQ RTNLSNTLLL LKSLDVTDEL SKFPFIDKPP
760 770 780 790 800
LQTFLSSLYE LWFIGAIDTS GQLTPLGLQM AKFPLQPSLS KILLIAVRNG
810 820 830 840 850
CSDEMLTIVS MLSVPQVFYR PKERQKEADI ARNKFFIAKS DHLTLLNVFE
860 870 880 890 900
QWRANNFSSH WCNKHFVQYK SLVRARDIRD QLLTILKSQK IPVISSGKDW
910 920 930 940 950
DIIKKCICSG FAHQAAKITG LRNYVHLKTG VSVQLHPTSA LHGLGDLPPY
960 970 980 990 1000
VVYHELLMTS KEYICCVTSV DPFWLMEYGG LLYDIKRIKN DQEATTTGLF
1010 1020 1030 1040 1050
GEHYEHTLDK VEDDIDINIR RCKDMRDSVI QELKMTDNSN KEDKKQKTKK
1060 1070
QNILNGKENS MKPFKRRKPF F
Length:1,071
Mass (Da):121,653
Last modified:June 1, 1994 - v2
Checksum:iEB7D4C4A18F5D4E1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti698 – 6981A → R in AAA34911 (PubMed:2138057).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31524 Genomic DNA. Translation: AAA34911.1.
Z27116 Genomic DNA. Translation: CAA81637.1.
Z28311 Genomic DNA. Translation: CAA82165.1.
BK006944 Genomic DNA. Translation: DAA09236.1.
PIRiS38164.
RefSeqiNP_013012.3. NM_001179876.3.

Genome annotation databases

EnsemblFungiiYKR086W; YKR086W; YKR086W.
GeneIDi853961.
KEGGisce:YKR086W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31524 Genomic DNA. Translation: AAA34911.1.
Z27116 Genomic DNA. Translation: CAA81637.1.
Z28311 Genomic DNA. Translation: CAA82165.1.
BK006944 Genomic DNA. Translation: DAA09236.1.
PIRiS38164.
RefSeqiNP_013012.3. NM_001179876.3.

3D structure databases

ProteinModelPortaliP15938.
SMRiP15938. Positions 310-989.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34217. 143 interactions.
DIPiDIP-2824N.
IntActiP15938. 20 interactions.
MINTiMINT-1699731.

PTM databases

iPTMnetiP15938.

Proteomic databases

MaxQBiP15938.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR086W; YKR086W; YKR086W.
GeneIDi853961.
KEGGisce:YKR086W.

Organism-specific databases

EuPathDBiFungiDB:YKR086W.
SGDiS000001794. PRP16.

Phylogenomic databases

GeneTreeiENSGT00840000130110.
HOGENOMiHOG000175261.
InParanoidiP15938.
KOiK12815.
OMAiITSATMN.
OrthoDBiEOG7M98QW.

Enzyme and pathway databases

BioCyciYEAST:G3O-32049-MONOMER.

Miscellaneous databases

PROiP15938.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing."
    Burgess S., Couto J.R., Guthrie C.
    Cell 60:705-717(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
    Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
    Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome."
    Schwer B., Guthrie C.
    Nature 349:494-499(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRP16_YEAST
AccessioniPrimary (citable) accession number: P15938
Secondary accession number(s): D6VXE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.