P15937 (ACH1_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-CoA hydrolase
EC=3.1.2.1

Alternative name(s):
Acetate utilization protein
Acetyl-CoA deacylase
Short name=Acetyl-CoA acylase

Gene names

Name:	acu-8
ORF Names:	NCU09770

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › Neurospora crassa

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for utilization of acetate. Ref.3
Catalytic activity	Acetyl-CoA + H2O = CoA + acetate.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the acetyl-CoA hydrolase/transferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	acetate metabolic process Inferred from electronic annotation. Source: EnsemblFungi acetyl-CoA metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from electronic annotation. Source: EnsemblFungi mitochondrion Inferred from electronic annotation. Source: EnsemblFungi
Molecular_function	acetate CoA-transferase activity Inferred from electronic annotation. Source: EnsemblFungi acetyl-CoA hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Acetyl-CoA hydrolase		PRO_0000215521
Experimental info
	Sequence conflict	14 – 16	3	VKR → QG in AAA33554. Ref.1
	Sequence conflict	46 – 56	11	GVGYPKKVPTM → ASATPRRSYY in AAA33554. Ref.1
	Sequence conflict	65 – 86	22	GLQGQ…AETEN → ASRPAQVQPLRRCLRRRRDR E in AAA33554. Ref.1
	Sequence conflict	95	1	A → AHDR in AAA33554. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P15937 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: E6134462D4A35423	FASTA	525	58,006
	10 20 30 40 50 60 MASPIASAAL RARVKRPSML KKLCNPEDML QHFPNGAYIG WSGFTGVGYP KKVPTMLADH 70 80 90 100 110 120 VEKNGLQGQL KYSLFVGASA GAETENRWAA LDMIARRAPH QVGKNIAKGI NEGRINFFDK 130 140 150 160 170 180 HLSMFPVDLV YGYYTKDRQN KNLDVVCVEA TEIKEDGSIV LGASVGATPE LIQMADKVII 190 200 210 220 230 240 EVNTAIPSFD GLHDITFSDL PPNRKPYLIQ QCRDRIGTTS VPVDPEKVVG IIECTTPDQT 250 260 270 280 290 300 LPNSPADETA TAIAGHLIEF FEHEVAHGRL PKNLLPLQSG IGNIANAVIG GLETSNFKNL 310 320 330 340 350 360 NVWTEVIQDT FLDLFDSGKL DFATATSIRF SPTGFERFYK NWDNYYDKLL LRSQSVSNAP 370 380 390 400 410 420 EIIRRLGVIG MNTPVEVDIY AHANSTNVMG SRMLNGLGGS ADFLRNSKYS IMHTPSTRPS 430 440 450 460 470 480 KTDAHGVSCI VPMCTHVDQT EHDLDVIVTE NGLADVRGLS PRERARVIID KCAHDVYKPI 490 500 510 520 LKAYFEKAEF ECLRKGMGHE PHLLFNSFDM HKALVEEGSM AKVKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Duplication-induced mutation of a new Neurospora gene required for acetate utilization: properties of the mutant and predicted amino acid sequence of the protein product." Marathe S., Connerton I.F., Fincham J.R.S. Mol. Cell. Biol. 10:2638-2644(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"An acetate-sensitive mutant of Neurospora crassa deficient in acetyl-CoA hydrolase." Connerton I.F., McCullough W., Fincham J.R.S. J. Gen. Microbiol. 138:1797-1800(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31521 Genomic DNA. Translation: AAA33554.1. AABX02000087 Genomic DNA. Translation: EAA29118.1.
PIR	A36316.
RefSeq	XP_958354.1. XM_953261.2.
UniGene	Ncr.13786.
3D structure databases
ProteinModelPortal	P15937.
ModBase	Search...
Protein-protein interaction databases
STRING	5141.NCU09770.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000009606; EFNCRP00000009588; EFNCRG00000009587.
GeneID	3874501.
KEGG	ncr:NCU09770.
Phylogenomic databases
eggNOG	COG0427.
HOGENOM	HOG000172597.
KO	K01067.
OMA	EVSNHPE.
OrthoDB	EOG4DBXNW.
Family and domain databases
InterPro	IPR026888. AcetylCoA_hyd_C. IPR003702. ActCoA_hydro. IPR017821. Succinate_CoA_transferase. [Graphical view]
PANTHER	PTHR21432. PTHR21432. 1 hit. PTHR21432:SF10. PTHR21432:SF10. 1 hit.
Pfam	PF13336. AcetylCoA_hyd_C. 1 hit. PF02550. AcetylCoA_hydro. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACH1_NEUCR

Accession

Primary (citable) accession number: P15937
Secondary accession number(s): Q7RV87

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 4, 2005
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program