Reviewed, UniProtKB/Swiss-Prot P15922 (PEHX_ERWCH)

Names and origin

Protein names

Recommended name:
    Exo-poly-alpha-D-galacturonosidase
      Short name=Exo-PG
    EC=3.2.1.82

Gene names

Name:

pehX

Organism

Erwinia chrysanthemi

Taxonomic identifier

556 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Contributes significantly to bacterial utilization of polygalacturonate and the induction of pectate lyase in the presence of extracellular pectic polymers.
Catalytic activity	Hydrolysis of pectic acid from the non-reducing end, releasing digalacturonate.
Subcellular location	Secreted. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 1 fibronectin type-III domain. Contains 4 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-poly-alpha-galacturonosidase activity Inferred from electronic annotation. Source: EC polygalacturonase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.1
	Chain	28 – 602	575	Exo-poly-alpha-D-galacturonosidase		PRO_0000024824
Regions
	Domain	29 – 143	115	Fibronectin type-III
Sites
	Active site	395	1	Proton donor By similarity
	Active site	428	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15922-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: C7F44689C343B34B	FASTA	602	64,444
	10 20 30 40 50 60 MKVITFSRRS ALASIVATCL MSTPALAATA QAPQKLQIPT LSYDDHSVML VWDTPEDTSN 70 80 90 100 110 120 ITDYQIYQNG QLIGLASQNN DKNSPAKPYI SAFYKSDAAN FHHRIVLQNA KVDGLKAGTD 130 140 150 160 170 180 YQFTVRTVYA DGTTSNDSNT VTTTTTAVPK VINITQYGAK GDGTTLNTSA IQKAIDACPT 190 200 210 220 230 240 GCRIDVPAGV FKTGALWLKS DMTLNLLQGA TLLGSDNAAD YPDAYKIYSY VSQVRPASLL 250 260 270 280 290 300 NAIDKNSSAV GTFKNIRIVG KGIIDGNGWK RSADAKDELG NTLPQYVKSD NSKVSKDGIL 310 320 330 340 350 360 AKNQVAAAVA TGMDTKTAYS QRRSSLVTLR GVQNAYIADV TIRNPANHGI MFLESENVVE 370 380 390 400 410 420 NSVIHQTFNA NNGDGVEFGN SQNIMVFNSV FDTGDDSINF AAGMGQDAQK QEPSQNAWLF 430 440 450 460 470 480 NNFFRHGHGA VVLGSHTGAG IVDVLAENNV ITQNDVGLRA KSAPAIGGGA HGIVFRNSAM 490 500 510 520 530 540 KNLAKQAVIV TLSYADNNGT IDYTPAKVPA RFYDFTVKNV TVQDSTGSNP AIEITGDSSK 550 560 570 580 590 600 DIWHSQFIFS NMKLSGVSPT SISDLSDSQF NNLTFSNLRS GSSPWKFGTV KNVTVDGKTV TP

References

[1]	"Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of the exo-poly-alpha-D-galacturonosidase-encoding pehX gene of Erwinia chrysanthemi EC16." He S.Y., Collmer A. J. Bacteriol. 172:4988-4995(1990) [PubMed: 2168372] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-40, SUBCELLULAR LOCATION. Strain: EC16.
[2]	"Proposed acquisition of an animal protein domain by bacteria." Bork P., Doolittle R.F. Proc. Natl. Acad. Sci. U.S.A. 89:8990-8994(1992) [PubMed: 1409594] [Abstract] Cited for: DOMAIN FIBRONECTIN TYPE-III.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M31308 Genomic DNA. Translation: AAA24842.1.
PIR	A36715.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.82. 1459.
Family and domain databases
InterPro	IPR003961. FN_III. IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00041. fn3. 1 hit. PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00060. FN3. 1 hit. SM00710. PbH1. 3 hits. [Graphical view]
PROSITE	PS50853. FN3. 1 hit. PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEHX_ERWCH

Accession

Primary (citable) accession number: P15922

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)