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Protein

V-type proton ATPase 116 kDa subunit a isoform 2

Gene

Atp6v0a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the proton channel of V-ATPases (By similarity). Essential component of the endosomal pH-sensing machinery. May play a role in maintaining the Golgi functions, such as glycosylation maturation, by controlling the Golgi pH.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-77387. Insulin receptor recycling.
R-MMU-917977. Transferrin endocytosis and recycling.
R-MMU-983712. Ion channel transport.

Protein family/group databases

TCDBi3.A.2.2.6. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase 116 kDa subunit a isoform 2
Short name:
V-ATPase 116 kDa isoform a2
Alternative name(s):
Immune suppressor factor J6B7
Short name:
ISF
Lysosomal H(+)-transporting ATPase V0 subunit a2
ShIF
Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2
Gene namesi
Name:Atp6v0a2
Synonyms:Atp6n1b, Tj6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:104855. Atp6v0a2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 393CytoplasmicSequence analysisAdd BLAST393
Transmembranei394 – 412HelicalSequence analysisAdd BLAST19
Topological domaini413 – 414VacuolarSequence analysis2
Transmembranei415 – 431HelicalSequence analysisAdd BLAST17
Topological domaini432 – 445CytoplasmicSequence analysisAdd BLAST14
Transmembranei446 – 475HelicalSequence analysisAdd BLAST30
Topological domaini476 – 549VacuolarSequence analysisAdd BLAST74
Transmembranei550 – 569HelicalSequence analysisAdd BLAST20
Topological domaini570 – 587CytoplasmicSequence analysisAdd BLAST18
Transmembranei588 – 608HelicalSequence analysisAdd BLAST21
Topological domaini609 – 651VacuolarSequence analysisAdd BLAST43
Transmembranei652 – 671HelicalSequence analysisAdd BLAST20
Topological domaini672 – 739CytoplasmicSequence analysisAdd BLAST68
Transmembranei740 – 764HelicalSequence analysisAdd BLAST25
Topological domaini765 – 785VacuolarSequence analysisAdd BLAST21
Transmembranei786 – 824HelicalSequence analysisAdd BLAST39
Topological domaini825 – 856CytoplasmicSequence analysisAdd BLAST32

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192171 – 856V-type proton ATPase 116 kDa subunit a isoform 2Add BLAST856

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei695PhosphoserineCombined sources1
Modified residuei700PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP15920.
MaxQBiP15920.
PaxDbiP15920.
PeptideAtlasiP15920.
PRIDEiP15920.

PTM databases

iPTMnetiP15920.
PhosphoSitePlusiP15920.
SwissPalmiP15920.

Expressioni

Tissue specificityi

Relatively high expression in kidney and liver. Lower levels in the spleen, testis, and skeletal muscle. Also expressed in the thymus.1 Publication

Gene expression databases

BgeeiENSMUSG00000038023.
CleanExiMM_ATP6V0A2.
ExpressionAtlasiP15920. baseline and differential.
GenevisibleiP15920. MM.

Interactioni

Subunit structurei

The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''. Directly interacts with PSCD2 through its N-terminal cytosolic tail in an intra-endosomal acidification-dependent manner. Disruption of this interaction results in the inhibition of endocytosis.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Cyth2P630345EBI-988456,EBI-988425

GO - Molecular functioni

Protein-protein interaction databases

IntActiP15920. 1 interactor.
STRINGi10090.ENSMUSP00000039737.

Structurei

Secondary structure

1856
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 16Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LX4NMR-A1-17[»]
ProteinModelPortaliP15920.
SMRiP15920.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the V-ATPase 116 kDa subunit family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2189. Eukaryota.
COG1269. LUCA.
GeneTreeiENSGT00390000004941.
HOGENOMiHOG000037059.
HOVERGENiHBG014606.
InParanoidiP15920.
KOiK02154.
OMAiHPPAEHK.
OrthoDBiEOG091G01BI.
PhylomeDBiP15920.
TreeFamiTF300346.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 1 hit.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P15920-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSLFRSESM CLAQLFLQSG TAYECLSALG EKGLVQFRDL NQNVSSFQRK
60 70 80 90 100
FVGEVKRCEE LERILVYLVQ EITRADIPLP EGEASPPAPP LKHVLEMQEQ
110 120 130 140 150
LQKLEVELRE VTKNKEKLRK NLLELVEYTH MLRVTKTFLK RNVEFEPTYE
160 170 180 190 200
EFPALENDSL LDYSCMQRLG AKLGFVSGLI QQGRVEAFER MLWRACKGYT
210 220 230 240 250
IVTYAELDEC LEDPETGEVI KWYVFLISFW GEQIGHKVKK ICDCYHCHIY
260 270 280 290 300
PYPNTAEERR EIQEGLNTRI QDLYTVLHKT EDYLRQVLCK AAESVCSRVV
310 320 330 340 350
QVRKMKAIYH MLNMCSFDVT NKCLIAEVWC PEVDLPGLRR ALEEGSRESG
360 370 380 390 400
ATIPSFMNTI PTKETPPTLI RTNKFTEGFQ NIVDAYGVGS YREVNPALFT
410 420 430 440 450
IITFPFLFAV MFGDFGHGFV MFLFALLLVL NENHPRLSQS QEILRMFFDG
460 470 480 490 500
RYILLLMGLF SVYTGLIYND CFSKSVNLFG SGWNVSAMYS SSHSPEEQRK
510 520 530 540 550
MVLWNDSTIR HSRTLQLDPN IPGVFRGPYP FGIDPIWNLA TNRLTFLNSF
560 570 580 590 600
KMKMSVILGI FHMTFGVVLG IFNHLHFRKK FNVYLVSVPE ILFMLCIFGY
610 620 630 640 650
LIFMIIYKWL AYSAETSREA PSILIEFINM FLFPTSKTHG LYPGQAHVQR
660 670 680 690 700
VLVALTVLAV PVLFLGKPLF LLWLHNGRNC FGMSRSGYTL VRKDSEEEVS
710 720 730 740 750
LLGNQDIEEG NSRMEEGCRE VTCEEFNFGE ILMTQAIHSI EYCLGCISNT
760 770 780 790 800
ASYLRLWALS LAHAQLSDVL WAMLMRVGLR VDTTYGVLLL LPVMAFFAVL
810 820 830 840 850
TIFILLVMEG LSAFLHAIRL HWVEFQNKFY VGAGTKFVPF SFSLLSSKFS

NDDSIA
Length:856
Mass (Da):98,145
Last modified:January 11, 2001 - v2
Checksum:i6A0D593F6F401E22
GO
Isoform 2 (identifier: P15920-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-593: Missing.

Note: No experimental confirmation available.
Show »
Length:263
Mass (Da):29,740
Checksum:i0A1BDE2F82C0C02C
GO

Sequence cautioni

The sequence AAL57303 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti486S → C in AAA39336 (PubMed:2247090).Curated1
Sequence conflicti486S → C in CAA38968 (PubMed:2247090).Curated1
Sequence conflicti791Missing in AAA39336 (PubMed:2247090).Curated1
Sequence conflicti791Missing in CAA38968 (PubMed:2247090).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0320881 – 593Missing in isoform 2. 1 PublicationAdd BLAST593

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31226 mRNA. Translation: AAA39336.1.
X55184 mRNA. Translation: CAA38968.1.
AB022323 mRNA. Translation: BAA93007.1.
AF218252 mRNA. Translation: AAF59921.1.
AK032909 mRNA. Translation: BAC28081.1.
AK155055 mRNA. Translation: BAE33017.1.
BC108991 mRNA. Translation: AAI08992.1.
BC108992 mRNA. Translation: AAI08993.1.
BC112905 mRNA. Translation: AAI12906.1.
AF388674 mRNA. Translation: AAL57303.1. Different initiation.
PIRiJH0287.
RefSeqiNP_035726.2. NM_011596.5. [P15920-1]
UniGeneiMm.1158.
Mm.392098.
Mm.490281.

Genome annotation databases

EnsembliENSMUST00000037865; ENSMUSP00000039737; ENSMUSG00000038023. [P15920-1]
GeneIDi21871.
KEGGimmu:21871.
UCSCiuc008zqn.2. mouse. [P15920-2]
uc029voj.2. mouse. [P15920-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31226 mRNA. Translation: AAA39336.1.
X55184 mRNA. Translation: CAA38968.1.
AB022323 mRNA. Translation: BAA93007.1.
AF218252 mRNA. Translation: AAF59921.1.
AK032909 mRNA. Translation: BAC28081.1.
AK155055 mRNA. Translation: BAE33017.1.
BC108991 mRNA. Translation: AAI08992.1.
BC108992 mRNA. Translation: AAI08993.1.
BC112905 mRNA. Translation: AAI12906.1.
AF388674 mRNA. Translation: AAL57303.1. Different initiation.
PIRiJH0287.
RefSeqiNP_035726.2. NM_011596.5. [P15920-1]
UniGeneiMm.1158.
Mm.392098.
Mm.490281.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LX4NMR-A1-17[»]
ProteinModelPortaliP15920.
SMRiP15920.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP15920. 1 interactor.
STRINGi10090.ENSMUSP00000039737.

Protein family/group databases

TCDBi3.A.2.2.6. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

PTM databases

iPTMnetiP15920.
PhosphoSitePlusiP15920.
SwissPalmiP15920.

Proteomic databases

EPDiP15920.
MaxQBiP15920.
PaxDbiP15920.
PeptideAtlasiP15920.
PRIDEiP15920.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037865; ENSMUSP00000039737; ENSMUSG00000038023. [P15920-1]
GeneIDi21871.
KEGGimmu:21871.
UCSCiuc008zqn.2. mouse. [P15920-2]
uc029voj.2. mouse. [P15920-1]

Organism-specific databases

CTDi23545.
MGIiMGI:104855. Atp6v0a2.

Phylogenomic databases

eggNOGiKOG2189. Eukaryota.
COG1269. LUCA.
GeneTreeiENSGT00390000004941.
HOGENOMiHOG000037059.
HOVERGENiHBG014606.
InParanoidiP15920.
KOiK02154.
OMAiHPPAEHK.
OrthoDBiEOG091G01BI.
PhylomeDBiP15920.
TreeFamiTF300346.

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-77387. Insulin receptor recycling.
R-MMU-917977. Transferrin endocytosis and recycling.
R-MMU-983712. Ion channel transport.

Miscellaneous databases

ChiTaRSiAtp6v0a2. mouse.
PROiP15920.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038023.
CleanExiMM_ATP6V0A2.
ExpressionAtlasiP15920. baseline and differential.
GenevisibleiP15920. MM.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 1 hit.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVPP2_MOUSE
AccessioniPrimary (citable) accession number: P15920
Secondary accession number(s): A4FU82
, Q3U2X3, Q8VHU0, Q9JHJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 11, 2001
Last modified: November 2, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.