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Protein

Beta-galactoside alpha-2,6-sialyltransferase 1

Gene

ST6GAL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.2 Publications

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.2 Publications

Enzyme regulationi

Inhibited by CTP.1 Publication

Kineticsi

  1. KM=530 µM for CMP-NeuAc1 Publication
  1. Vmax=1.074 pmol/min/µg enzyme1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189Substrate; via amide nitrogen1
Binding sitei212Substrate1
Binding sitei233Substrate1
Binding sitei353Substrate; via carbonyl oxygen1
Binding sitei354Substrate1
Binding sitei365Substrate1
Binding sitei369SubstrateCurated1
Binding sitei370Substrate1
Binding sitei376Substrate1

GO - Molecular functioni

  • beta-galactoside alpha-2,6-sialyltransferase activity Source: UniProtKB
  • sialyltransferase activity Source: UniProtKB

GO - Biological processi

  • humoral immune response Source: ProtInc
  • N-acetylneuraminate metabolic process Source: UniProtKB
  • O-glycan processing Source: Reactome
  • oligosaccharide metabolic process Source: GO_Central
  • protein N-linked glycosylation via asparagine Source: UniProtKB
  • sialylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS01118-MONOMER.
ZFISH:HS01118-MONOMER.
BRENDAi2.4.99.1. 2681.
ReactomeiR-HSA-4085001. Sialic acid metabolism.
R-HSA-975577. N-Glycan antennae elongation.
R-HSA-977068. Termination of O-glycan biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactoside alpha-2,6-sialyltransferase 1 (EC:2.4.99.1)
Short name:
Alpha 2,6-ST 1
Alternative name(s):
B-cell antigen CD75
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
ST6Gal I
Short name:
ST6GalI
Sialyltransferase 1
Gene namesi
Name:ST6GAL1
Synonyms:SIAT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:10860. ST6GAL1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9Cytoplasmic9
Transmembranei10 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini27 – 406LumenalSequence analysisAdd BLAST380

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi6480.
OpenTargetsiENSG00000073849.
PharmGKBiPA35762.

Chemistry databases

ChEMBLiCHEMBL3596075.

Polymorphism and mutation databases

BioMutaiST6GAL1.
DMDMi115445.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492491 – 406Beta-galactoside alpha-2,6-sialyltransferase 1Add BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi142 ↔ 4061 Publication
Glycosylationi149N-linked (GlcNAc...)2 Publications1
Glycosylationi161N-linked (GlcNAc...)1 Publication1
Disulfide bondi184 ↔ 3351 Publication
Disulfide bondi353 ↔ 3641 Publication
Modified residuei369PhosphotyrosineCombined sources1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.
The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP15907.
MaxQBiP15907.
PaxDbiP15907.
PeptideAtlasiP15907.
PRIDEiP15907.

PTM databases

iPTMnetiP15907.
PhosphoSitePlusiP15907.

Expressioni

Gene expression databases

BgeeiENSG00000073849.
CleanExiHS_ST6GAL1.
ExpressionAtlasiP15907. baseline and differential.
GenevisibleiP15907. HS.

Organism-specific databases

HPAiCAB015018.
CAB016122.

Interactioni

Protein-protein interaction databases

BioGridi112374. 17 interactors.
IntActiP15907. 2 interactors.
MINTiMINT-5000509.
STRINGi9606.ENSP00000169298.

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi102 – 104Combined sources3
Helixi107 – 118Combined sources12
Helixi137 – 147Combined sources11
Turni158 – 161Combined sources4
Helixi163 – 165Combined sources3
Turni166 – 168Combined sources3
Helixi174 – 177Combined sources4
Beta strandi181 – 187Combined sources7
Helixi191 – 193Combined sources3
Helixi199 – 202Combined sources4
Beta strandi205 – 211Combined sources7
Helixi220 – 223Combined sources4
Beta strandi228 – 233Combined sources6
Helixi234 – 239Combined sources6
Helixi241 – 244Combined sources4
Helixi247 – 250Combined sources4
Beta strandi251 – 257Combined sources7
Helixi266 – 271Combined sources6
Helixi278 – 287Combined sources10
Beta strandi293 – 296Combined sources4
Helixi299 – 311Combined sources13
Beta strandi312 – 314Combined sources3
Helixi323 – 334Combined sources12
Beta strandi335 – 344Combined sources10
Beta strandi354 – 356Combined sources3
Helixi363 – 366Combined sources4
Beta strandi368 – 370Combined sources3
Helixi372 – 382Combined sources11
Helixi387 – 393Combined sources7
Beta strandi395 – 399Combined sources5
Helixi401 – 403Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JS1X-ray2.09A89-406[»]
4JS2X-ray2.30A89-406[»]
ProteinModelPortaliP15907.
SMRiP15907.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni322 – 324Substrate binding3

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074444.
HOGENOMiHOG000013206.
HOVERGENiHBG052853.
InParanoidiP15907.
KOiK00778.
OMAiSFQVWNK.
OrthoDBiEOG091G07C7.
PhylomeDBiP15907.
TreeFamiTF323961.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P15907-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIHTNLKKKF SCCVLVFLLF AVICVWKEKK KGSYYDSFKL QTKEFQVLKS
60 70 80 90 100
LGKLAMGSDS QSVSSSSTQD PHRGRQTLGS LRGLAKAKPE ASFQVWNKDS
110 120 130 140 150
SSKNLIPRLQ KIWKNYLSMN KYKVSYKGPG PGIKFSAEAL RCHLRDHVNV
160 170 180 190 200
SMVEVTDFPF NTSEWEGYLP KESIRTKAGP WGRCAVVSSA GSLKSSQLGR
210 220 230 240 250
EIDDHDAVLR FNGAPTANFQ QDVGTKTTIR LMNSQLVTTE KRFLKDSLYN
260 270 280 290 300
EGILIVWDPS VYHSDIPKWY QNPDYNFFNN YKTYRKLHPN QPFYILKPQM
310 320 330 340 350
PWELWDILQE ISPEEIQPNP PSSGMLGIII MMTLCDQVDI YEFLPSKRKT
360 370 380 390 400
DVCYYYQKFF DSACTMGAYH PLLYEKNLVK HLNQGTDEDI YLLGKATLPG

FRTIHC
Length:406
Mass (Da):46,605
Last modified:April 1, 1990 - v1
Checksum:iAC1E24A3875CF00F
GO
Isoform 2 (identifier: P15907-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-231: Missing.

Note: No experimental confirmation available.
Show »
Length:175
Mass (Da):20,764
Checksum:i2A31915E3D582F4B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27K → L in CAA38246 (PubMed:2373995).Curated1
Sequence conflicti72 – 73HR → T in CAA38246 (PubMed:2373995).Curated2
Sequence conflicti144L → P in CAA38246 (PubMed:2373995).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0560761 – 231Missing in isoform 2. 1 PublicationAdd BLAST231

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17247 mRNA. Translation: CAA35111.1.
X54363 mRNA. Translation: CAA38246.1.
X62822 mRNA. Translation: CAA44634.1.
AK292879 mRNA. Translation: BAF85568.1.
AK312023 mRNA. Translation: BAG34960.1.
AC007488 Genomic DNA. No translation available.
AC007690 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78160.1.
CH471052 Genomic DNA. Translation: EAW78161.1.
CH471052 Genomic DNA. Translation: EAW78162.1.
CH471052 Genomic DNA. Translation: EAW78164.1.
BC031476 mRNA. Translation: AAH31476.1.
BC040009 mRNA. Translation: AAH40009.1.
CCDSiCCDS3285.1. [P15907-1]
CCDS46973.1. [P15907-2]
PIRiA41734.
RefSeqiNP_003023.1. NM_003032.2. [P15907-1]
NP_775323.1. NM_173216.2. [P15907-1]
NP_775324.1. NM_173217.2. [P15907-2]
XP_005247776.1. XM_005247719.1. [P15907-1]
XP_005247777.1. XM_005247720.1. [P15907-1]
XP_006713797.1. XM_006713734.1. [P15907-1]
XP_011511387.1. XM_011513085.1. [P15907-1]
XP_011511388.1. XM_011513086.1. [P15907-1]
XP_016862554.1. XM_017007065.1. [P15907-1]
XP_016862555.1. XM_017007066.1. [P15907-1]
XP_016862556.1. XM_017007067.1. [P15907-1]
XP_016862557.1. XM_017007068.1. [P15907-1]
UniGeneiHs.207459.

Genome annotation databases

EnsembliENST00000169298; ENSP00000169298; ENSG00000073849. [P15907-1]
ENST00000448044; ENSP00000389337; ENSG00000073849. [P15907-1]
ENST00000457772; ENSP00000412221; ENSG00000073849. [P15907-2]
GeneIDi6480.
KEGGihsa:6480.
UCSCiuc003frb.4. human. [P15907-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST6Gal I

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17247 mRNA. Translation: CAA35111.1.
X54363 mRNA. Translation: CAA38246.1.
X62822 mRNA. Translation: CAA44634.1.
AK292879 mRNA. Translation: BAF85568.1.
AK312023 mRNA. Translation: BAG34960.1.
AC007488 Genomic DNA. No translation available.
AC007690 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78160.1.
CH471052 Genomic DNA. Translation: EAW78161.1.
CH471052 Genomic DNA. Translation: EAW78162.1.
CH471052 Genomic DNA. Translation: EAW78164.1.
BC031476 mRNA. Translation: AAH31476.1.
BC040009 mRNA. Translation: AAH40009.1.
CCDSiCCDS3285.1. [P15907-1]
CCDS46973.1. [P15907-2]
PIRiA41734.
RefSeqiNP_003023.1. NM_003032.2. [P15907-1]
NP_775323.1. NM_173216.2. [P15907-1]
NP_775324.1. NM_173217.2. [P15907-2]
XP_005247776.1. XM_005247719.1. [P15907-1]
XP_005247777.1. XM_005247720.1. [P15907-1]
XP_006713797.1. XM_006713734.1. [P15907-1]
XP_011511387.1. XM_011513085.1. [P15907-1]
XP_011511388.1. XM_011513086.1. [P15907-1]
XP_016862554.1. XM_017007065.1. [P15907-1]
XP_016862555.1. XM_017007066.1. [P15907-1]
XP_016862556.1. XM_017007067.1. [P15907-1]
XP_016862557.1. XM_017007068.1. [P15907-1]
UniGeneiHs.207459.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JS1X-ray2.09A89-406[»]
4JS2X-ray2.30A89-406[»]
ProteinModelPortaliP15907.
SMRiP15907.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112374. 17 interactors.
IntActiP15907. 2 interactors.
MINTiMINT-5000509.
STRINGi9606.ENSP00000169298.

Chemistry databases

ChEMBLiCHEMBL3596075.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiP15907.
PhosphoSitePlusiP15907.

Polymorphism and mutation databases

BioMutaiST6GAL1.
DMDMi115445.

Proteomic databases

EPDiP15907.
MaxQBiP15907.
PaxDbiP15907.
PeptideAtlasiP15907.
PRIDEiP15907.

Protocols and materials databases

DNASUi6480.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000169298; ENSP00000169298; ENSG00000073849. [P15907-1]
ENST00000448044; ENSP00000389337; ENSG00000073849. [P15907-1]
ENST00000457772; ENSP00000412221; ENSG00000073849. [P15907-2]
GeneIDi6480.
KEGGihsa:6480.
UCSCiuc003frb.4. human. [P15907-1]

Organism-specific databases

CTDi6480.
DisGeNETi6480.
GeneCardsiST6GAL1.
HGNCiHGNC:10860. ST6GAL1.
HPAiCAB015018.
CAB016122.
MIMi109675. gene.
neXtProtiNX_P15907.
OpenTargetsiENSG00000073849.
PharmGKBiPA35762.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074444.
HOGENOMiHOG000013206.
HOVERGENiHBG052853.
InParanoidiP15907.
KOiK00778.
OMAiSFQVWNK.
OrthoDBiEOG091G07C7.
PhylomeDBiP15907.
TreeFamiTF323961.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:HS01118-MONOMER.
ZFISH:HS01118-MONOMER.
BRENDAi2.4.99.1. 2681.
ReactomeiR-HSA-4085001. Sialic acid metabolism.
R-HSA-975577. N-Glycan antennae elongation.
R-HSA-977068. Termination of O-glycan biosynthesis.

Miscellaneous databases

ChiTaRSiST6GAL1. human.
GeneWikiiST6GAL1.
GenomeRNAii6480.
PROiP15907.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000073849.
CleanExiHS_ST6GAL1.
ExpressionAtlasiP15907. baseline and differential.
GenevisibleiP15907. HS.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIAT1_HUMAN
AccessioniPrimary (citable) accession number: P15907
Secondary accession number(s): A8KA14, B2R513, D3DNV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 179 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.