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Protein

Oxygen-insensitive NAD(P)H nitroreductase

Gene

nfsB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone (By similarity).By similarity

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41NAD or NADP; via amide nitrogenBy similarity1
Binding sitei71FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 14FMNBy similarity5
Nucleotide bindingi153 – 158NAD or NADPBy similarity6
Nucleotide bindingi165 – 166FMNBy similarity2
Nucleotide bindingi205 – 207FMNBy similarity3

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-insensitive NAD(P)H nitroreductase (EC:1.-.-.-)
Gene namesi
Name:nfsBBy similarity
Synonyms:nfnBImported, nfsI
Ordered Locus Names:STM0578
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000727131 – 217Oxygen-insensitive NAD(P)H nitroreductaseAdd BLAST217

Proteomic databases

PaxDbiP15888.
PRIDEiP15888.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi99287.STM0578.

Structurei

Secondary structure

1217
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 9Combined sources7
Helixi24 – 36Combined sources13
Helixi40 – 42Combined sources3
Beta strandi46 – 51Combined sources6
Helixi54 – 61Combined sources8
Helixi62 – 64Combined sources3
Helixi66 – 71Combined sources6
Helixi72 – 77Combined sources6
Beta strandi78 – 89Combined sources12
Helixi92 – 104Combined sources13
Helixi111 – 129Combined sources19
Turni130 – 132Combined sources3
Helixi135 – 156Combined sources22
Helixi169 – 175Combined sources7
Helixi178 – 181Combined sources4
Beta strandi183 – 192Combined sources10
Helixi199 – 202Combined sources4
Helixi210 – 213Combined sources4
Beta strandi214 – 217Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HZNX-ray2.40A/B/C/D/E/F/G/H1-217[»]
ProteinModelPortaliP15888.
SMRiP15888.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15888.

Family & Domainsi

Sequence similaritiesi

Belongs to the nitroreductase family.Curated

Phylogenomic databases

eggNOGiENOG4108RCM. Bacteria.
COG0778. LUCA.
HOGENOMiHOG000146740.
KOiK10679.
OMAiCLAEEGL.
PhylomeDBiP15888.

Family and domain databases

CDDicd02149. NfsB_like_nitroreductase. 1 hit.
Gene3Di3.40.109.10. 1 hit.
InterProiIPR033878. NfsB-like.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.

Sequencei

Sequence statusi: Complete.

P15888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIVSVALQR YSTKAFDPSK KLTAEEADKI KTLLQYSPSS TNSQPWHFIV
60 70 80 90 100
ASTEEGKARV AKSAAGNYTF NERKMLDASH VVVFCAKTAM DDAWLERVVD
110 120 130 140 150
QEDADGRFAT PEAKAANDKG RRFFADMHRV SLKDDHQWMA KQVYLNVGNF
160 170 180 190 200
LLGVAAMGLD AVPIEGFDAE VLDAEFGLKE KGYTSLVVVP VGHHSVEDFN
210
AGLPKSRLPL ETTLTEV
Length:217
Mass (Da):23,955
Last modified:April 1, 1990 - v1
Checksum:iE90E9E05A0826D0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17250 Genomic DNA. Translation: CAA35113.1.
AE006468 Genomic DNA. Translation: AAL19529.1.
PIRiS08397.
RefSeqiNP_459570.1. NC_003197.1.
WP_000355879.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19529; AAL19529; STM0578.
GeneIDi1252098.
KEGGistm:STM0578.
PATRICi32379501. VBISalEnt20916_0610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17250 Genomic DNA. Translation: CAA35113.1.
AE006468 Genomic DNA. Translation: AAL19529.1.
PIRiS08397.
RefSeqiNP_459570.1. NC_003197.1.
WP_000355879.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HZNX-ray2.40A/B/C/D/E/F/G/H1-217[»]
ProteinModelPortaliP15888.
SMRiP15888.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0578.

Proteomic databases

PaxDbiP15888.
PRIDEiP15888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19529; AAL19529; STM0578.
GeneIDi1252098.
KEGGistm:STM0578.
PATRICi32379501. VBISalEnt20916_0610.

Phylogenomic databases

eggNOGiENOG4108RCM. Bacteria.
COG0778. LUCA.
HOGENOMiHOG000146740.
KOiK10679.
OMAiCLAEEGL.
PhylomeDBiP15888.

Miscellaneous databases

EvolutionaryTraceiP15888.

Family and domain databases

CDDicd02149. NfsB_like_nitroreductase. 1 hit.
Gene3Di3.40.109.10. 1 hit.
InterProiIPR033878. NfsB-like.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNFSB_SALTY
AccessioniPrimary (citable) accession number: P15888
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.