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Protein

Oxygen-insensitive NAD(P)H nitroreductase

Gene

nfsB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone (By similarity).By similarity

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411NAD or NADP; via amide nitrogenBy similarity
Binding sitei71 – 711FMNBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 145FMNBy similarity
Nucleotide bindingi153 – 1586NAD or NADPBy similarity
Nucleotide bindingi165 – 1662FMNBy similarity
Nucleotide bindingi205 – 2073FMNBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NAD, NADP

Enzyme and pathway databases

BioCyciSENT99287:GCTI-581-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-insensitive NAD(P)H nitroreductase (EC:1.-.-.-)
Gene namesi
Name:nfsBBy similarity
Synonyms:nfnBImported, nfsI
Ordered Locus Names:STM0578
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217Oxygen-insensitive NAD(P)H nitroreductasePRO_0000072713Add
BLAST

Proteomic databases

PaxDbiP15888.
PRIDEiP15888.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi99287.STM0578.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 97Combined sources
Helixi24 – 3613Combined sources
Helixi40 – 423Combined sources
Beta strandi46 – 516Combined sources
Helixi54 – 618Combined sources
Helixi62 – 643Combined sources
Helixi66 – 716Combined sources
Helixi72 – 776Combined sources
Beta strandi78 – 8912Combined sources
Helixi92 – 10413Combined sources
Helixi111 – 12919Combined sources
Turni130 – 1323Combined sources
Helixi135 – 15622Combined sources
Helixi169 – 1757Combined sources
Helixi178 – 1814Combined sources
Beta strandi183 – 19210Combined sources
Helixi199 – 2024Combined sources
Helixi210 – 2134Combined sources
Beta strandi214 – 2174Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HZNX-ray2.40A/B/C/D/E/F/G/H1-217[»]
ProteinModelPortaliP15888.
SMRiP15888. Positions 2-216.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15888.

Family & Domainsi

Sequence similaritiesi

Belongs to the nitroreductase family.Curated

Phylogenomic databases

eggNOGiENOG4108RCM. Bacteria.
COG0778. LUCA.
HOGENOMiHOG000146740.
KOiK10679.
OMAiCLAEEGL.
OrthoDBiEOG65QWJJ.
PhylomeDBiP15888.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
InterProiIPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.

Sequencei

Sequence statusi: Complete.

P15888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIVSVALQR YSTKAFDPSK KLTAEEADKI KTLLQYSPSS TNSQPWHFIV
60 70 80 90 100
ASTEEGKARV AKSAAGNYTF NERKMLDASH VVVFCAKTAM DDAWLERVVD
110 120 130 140 150
QEDADGRFAT PEAKAANDKG RRFFADMHRV SLKDDHQWMA KQVYLNVGNF
160 170 180 190 200
LLGVAAMGLD AVPIEGFDAE VLDAEFGLKE KGYTSLVVVP VGHHSVEDFN
210
AGLPKSRLPL ETTLTEV
Length:217
Mass (Da):23,955
Last modified:April 1, 1990 - v1
Checksum:iE90E9E05A0826D0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17250 Genomic DNA. Translation: CAA35113.1.
AE006468 Genomic DNA. Translation: AAL19529.1.
PIRiS08397.
RefSeqiNP_459570.1. NC_003197.1.
WP_000355879.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19529; AAL19529; STM0578.
GeneIDi1252098.
KEGGistm:STM0578.
PATRICi32379501. VBISalEnt20916_0610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17250 Genomic DNA. Translation: CAA35113.1.
AE006468 Genomic DNA. Translation: AAL19529.1.
PIRiS08397.
RefSeqiNP_459570.1. NC_003197.1.
WP_000355879.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HZNX-ray2.40A/B/C/D/E/F/G/H1-217[»]
ProteinModelPortaliP15888.
SMRiP15888. Positions 2-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0578.

Proteomic databases

PaxDbiP15888.
PRIDEiP15888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19529; AAL19529; STM0578.
GeneIDi1252098.
KEGGistm:STM0578.
PATRICi32379501. VBISalEnt20916_0610.

Phylogenomic databases

eggNOGiENOG4108RCM. Bacteria.
COG0778. LUCA.
HOGENOMiHOG000146740.
KOiK10679.
OMAiCLAEEGL.
OrthoDBiEOG65QWJJ.
PhylomeDBiP15888.

Enzyme and pathway databases

BioCyciSENT99287:GCTI-581-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP15888.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
InterProiIPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of Salmonella typhimurium nitroreductase gene."
    Watanabe M., Ishidate M., Nohmi T.
    Nucleic Acids Res. 18:1059-1059(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29631 / TA 1538.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiNFSB_SALTY
AccessioniPrimary (citable) accession number: P15888
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 11, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.