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Protein

Quinoprotein glucose dehydrogenase

Gene

gcd

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GDH is probably involved in energy conservation rather than in sugar metabolism.

Catalytic activityi

D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei466Proton acceptorCurated1

GO - Molecular functioni

  • magnesium ion binding Source: EcoCyc
  • pyrroloquinoline quinone binding Source: EcoCyc
  • quinoprotein glucose dehydrogenase activity Source: EcoCyc
  • ubiquinone binding Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
LigandPQQ

Enzyme and pathway databases

BioCyciEcoCyc:GLUCDEHYDROG-MONOMER
MetaCyc:GLUCDEHYDROG-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Quinoprotein glucose dehydrogenase (EC:1.1.5.2)
Alternative name(s):
Glucose dehydrogenase [pyrroloquinoline-quinone]
Gene namesi
Name:gcd
Ordered Locus Names:b0124, JW0120
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10369 gcd

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10Cytoplasmic1 Publication10
Transmembranei11 – 37HelicalCuratedAdd BLAST27
Topological domaini38 – 40Periplasmic1 Publication3
Transmembranei41 – 58HelicalCuratedAdd BLAST18
Topological domaini59 – 62Cytoplasmic1 Publication4
Transmembranei63 – 81HelicalCuratedAdd BLAST19
Topological domaini82 – 95Periplasmic1 PublicationAdd BLAST14
Transmembranei96 – 110HelicalCuratedAdd BLAST15
Topological domaini111 – 118Cytoplasmic1 Publication8
Transmembranei119 – 141HelicalCuratedAdd BLAST23
Topological domaini142 – 796Periplasmic1 PublicationAdd BLAST655

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002053391 – 796Quinoprotein glucose dehydrogenaseAdd BLAST796

Proteomic databases

EPDiP15877
PaxDbiP15877
PRIDEiP15877

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi426195811 interactors.
DIPiDIP-9747N
IntActiP15877 9 interactors.
STRINGi316385.ECDH10B_0104

Structurei

3D structure databases

ProteinModelPortaliP15877
SMRiP15877
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial PQQ dehydrogenase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DZG Bacteria
COG4993 LUCA
HOGENOMiHOG000116843
InParanoidiP15877
KOiK00117
OMAiLVWNWDS
PhylomeDBiP15877

Family and domain databases

CDDicd10280 PQQ_mGDH, 1 hit
InterProiView protein in InterPro
IPR018391 PQQ_beta_propeller_repeat
IPR017511 PQQ_mDH
IPR002372 PQQ_repeat
IPR011047 Quinoprotein_ADH-like_supfam
IPR001479 Quinoprotein_DH_CS
PANTHERiPTHR32303:SF4 PTHR32303:SF4, 1 hit
PfamiView protein in Pfam
PF01011 PQQ, 6 hits
SMARTiView protein in SMART
SM00564 PQQ, 6 hits
SUPFAMiSSF50998 SSF50998, 2 hits
TIGRFAMsiTIGR03074 PQQ_membr_DH, 1 hit
PROSITEiView protein in PROSITE
PS00363 BACTERIAL_PQQ_1, 1 hit
PS00364 BACTERIAL_PQQ_2, 1 hit

Sequencei

Sequence statusi: Complete.

P15877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAINNTGSRR LLVTLTALFA ALCGLYLLIG GGWLVAIGGS WYYPIAGLVM
60 70 80 90 100
LGVAWMLWRS KRAALWLYAA LLLGTMIWGV WEVGFDFWAL TPRSDILVFF
110 120 130 140 150
GIWLILPFVW RRLVIPASGA VAALVVALLI SGGILTWAGF NDPQEINGTL
160 170 180 190 200
SADATPAEAI SPVADQDWPA YGRNQEGQRF SPLKQINADN VHNLKEAWVF
210 220 230 240 250
RTGDVKQPND PGEITNEVTP IKVGDTLYLC TAHQRLFALD AASGKEKWHY
260 270 280 290 300
DPELKTNESF QHVTCRGVSY HEAKAETASP EVMADCPRRI ILPVNDGRLI
310 320 330 340 350
AINAENGKLC ETFANKGVLN LQSNMPDTKP GLYEPTSPPI ITDKTIVMAG
360 370 380 390 400
SVTDNFSTRE TSGVIRGFDV NTGELLWAFD PGAKDPNAIP SDEHTFTFNS
410 420 430 440 450
PNSWAPAAYD AKLDLVYLPM GVTTPDIWGG NRTPEQERYA SSILALNATT
460 470 480 490 500
GKLAWSYQTV HHDLWDMDLP AQPTLADITV NGQKVPVIYA PAKTGNIFVL
510 520 530 540 550
DRRNGELVVP APEKPVPQGA AKGDYVTPTQ PFSELSFRPT KDLSGADMWG
560 570 580 590 600
ATMFDQLVCR VMFHQMRYEG IFTPPSEQGT LVFPGNLGMF EWGGISVDPN
610 620 630 640 650
REVAIANPMA LPFVSKLIPR GPGNPMEQPK DAKGTGTESG IQPQYGVPYG
660 670 680 690 700
VTLNPFLSPF GLPCKQPAWG YISALDLKTN EVVWKKRIGT PQDSMPFPMP
710 720 730 740 750
VPVPFNMGMP MLGGPISTAG NVLFIAATAD NYLRAYNMSN GEKLWQGRLP
760 770 780 790
AGGQATPMTY EVNGKQYVVI SAGGHGSFGT KMGDYIVAYA LPDDVK
Length:796
Mass (Da):86,747
Last modified:November 1, 1997 - v3
Checksum:iD9EDC705A12894E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59R → L in CAA35706 (PubMed:2228962).Curated1
Sequence conflicti149 – 156TLSADATP → HLKRRCHT (PubMed:2228962).Curated8
Sequence conflicti149 – 156TLSADATP → HLKRRCHT (PubMed:8419307).Curated8
Sequence conflicti193N → K (PubMed:2228962).Curated1
Sequence conflicti193N → K (PubMed:8419307).Curated1
Sequence conflicti666Q → H in BAA02174 (PubMed:8419307).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51323 Genomic DNA Translation: CAA35706.1
D12651 Genomic DNA Translation: BAA02174.1
U00096 Genomic DNA Translation: AAC73235.1
AP009048 Genomic DNA Translation: BAB96699.1
PIRiD64735 JV0107
RefSeqiNP_414666.1, NC_000913.3
WP_001306211.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73235; AAC73235; b0124
BAB96699; BAB96699; BAB96699
GeneIDi944830
KEGGiecj:JW0120
eco:b0124
PATRICifig|1411691.4.peg.2158

Similar proteinsi

Entry informationi

Entry nameiDHG_ECOLI
AccessioniPrimary (citable) accession number: P15877
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 180 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome