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Protein

Quinoprotein glucose dehydrogenase

Gene

gcd

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GDH is probably involved in energy conservation rather than in sugar metabolism.

Catalytic activityi

D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei466 – 4661Proton acceptorCurated

GO - Molecular functioni

  • magnesium ion binding Source: EcoCyc
  • pyrroloquinoline quinone binding Source: EcoCyc
  • quinoprotein glucose dehydrogenase activity Source: EcoCyc
  • ubiquinone binding Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

PQQ

Enzyme and pathway databases

BioCyciEcoCyc:GLUCDEHYDROG-MONOMER.
ECOL316407:JW0120-MONOMER.
MetaCyc:GLUCDEHYDROG-MONOMER.
RETL1328306-WGS:GSTH-1225-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinoprotein glucose dehydrogenase (EC:1.1.5.2)
Alternative name(s):
Glucose dehydrogenase [pyrroloquinoline-quinone]
Gene namesi
Name:gcd
Ordered Locus Names:b0124, JW0120
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10369. gcd.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010Cytoplasmic1 Publication
Transmembranei11 – 3727HelicalCuratedAdd
BLAST
Topological domaini38 – 403Periplasmic1 Publication
Transmembranei41 – 5818HelicalCuratedAdd
BLAST
Topological domaini59 – 624Cytoplasmic1 Publication
Transmembranei63 – 8119HelicalCuratedAdd
BLAST
Topological domaini82 – 9514Periplasmic1 PublicationAdd
BLAST
Transmembranei96 – 11015HelicalCuratedAdd
BLAST
Topological domaini111 – 1188Cytoplasmic1 Publication
Transmembranei119 – 14123HelicalCuratedAdd
BLAST
Topological domaini142 – 796655Periplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 796796Quinoprotein glucose dehydrogenasePRO_0000205339Add
BLAST

Proteomic databases

EPDiP15877.
PaxDbiP15877.
PRIDEiP15877.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4261958. 8 interactions.
DIPiDIP-9747N.
IntActiP15877. 9 interactions.
MINTiMINT-1267882.
STRINGi511145.b0124.

Structurei

3D structure databases

ProteinModelPortaliP15877.
SMRiP15877. Positions 164-792.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial PQQ dehydrogenase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DZG. Bacteria.
COG4993. LUCA.
HOGENOMiHOG000116843.
InParanoidiP15877.
KOiK00117.
OMAiDVGETTY.
PhylomeDBiP15877.

Family and domain databases

CDDicd10280. PQQ_mGDH. 1 hit.
Gene3Di2.140.10.10. 2 hits.
InterProiIPR018391. PQQ_beta_propeller_repeat.
IPR017511. PQQ_mDH.
IPR002372. PQQ_repeat.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR001479. Quinoprotein_DH_CS.
[Graphical view]
PANTHERiPTHR32303:SF4. PTHR32303:SF4. 2 hits.
PfamiPF01011. PQQ. 6 hits.
[Graphical view]
SMARTiSM00564. PQQ. 6 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 2 hits.
TIGRFAMsiTIGR03074. PQQ_membr_DH. 1 hit.
PROSITEiPS00363. BACTERIAL_PQQ_1. 1 hit.
PS00364. BACTERIAL_PQQ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAINNTGSRR LLVTLTALFA ALCGLYLLIG GGWLVAIGGS WYYPIAGLVM
60 70 80 90 100
LGVAWMLWRS KRAALWLYAA LLLGTMIWGV WEVGFDFWAL TPRSDILVFF
110 120 130 140 150
GIWLILPFVW RRLVIPASGA VAALVVALLI SGGILTWAGF NDPQEINGTL
160 170 180 190 200
SADATPAEAI SPVADQDWPA YGRNQEGQRF SPLKQINADN VHNLKEAWVF
210 220 230 240 250
RTGDVKQPND PGEITNEVTP IKVGDTLYLC TAHQRLFALD AASGKEKWHY
260 270 280 290 300
DPELKTNESF QHVTCRGVSY HEAKAETASP EVMADCPRRI ILPVNDGRLI
310 320 330 340 350
AINAENGKLC ETFANKGVLN LQSNMPDTKP GLYEPTSPPI ITDKTIVMAG
360 370 380 390 400
SVTDNFSTRE TSGVIRGFDV NTGELLWAFD PGAKDPNAIP SDEHTFTFNS
410 420 430 440 450
PNSWAPAAYD AKLDLVYLPM GVTTPDIWGG NRTPEQERYA SSILALNATT
460 470 480 490 500
GKLAWSYQTV HHDLWDMDLP AQPTLADITV NGQKVPVIYA PAKTGNIFVL
510 520 530 540 550
DRRNGELVVP APEKPVPQGA AKGDYVTPTQ PFSELSFRPT KDLSGADMWG
560 570 580 590 600
ATMFDQLVCR VMFHQMRYEG IFTPPSEQGT LVFPGNLGMF EWGGISVDPN
610 620 630 640 650
REVAIANPMA LPFVSKLIPR GPGNPMEQPK DAKGTGTESG IQPQYGVPYG
660 670 680 690 700
VTLNPFLSPF GLPCKQPAWG YISALDLKTN EVVWKKRIGT PQDSMPFPMP
710 720 730 740 750
VPVPFNMGMP MLGGPISTAG NVLFIAATAD NYLRAYNMSN GEKLWQGRLP
760 770 780 790
AGGQATPMTY EVNGKQYVVI SAGGHGSFGT KMGDYIVAYA LPDDVK
Length:796
Mass (Da):86,747
Last modified:November 1, 1997 - v3
Checksum:iD9EDC705A12894E9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591R → L in CAA35706 (PubMed:2228962).Curated
Sequence conflicti149 – 1568TLSADATP → HLKRRCHT (PubMed:2228962).Curated
Sequence conflicti149 – 1568TLSADATP → HLKRRCHT (PubMed:8419307).Curated
Sequence conflicti193 – 1931N → K (PubMed:2228962).Curated
Sequence conflicti193 – 1931N → K (PubMed:8419307).Curated
Sequence conflicti666 – 6661Q → H in BAA02174 (PubMed:8419307).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51323 Genomic DNA. Translation: CAA35706.1.
D12651 Genomic DNA. Translation: BAA02174.1.
U00096 Genomic DNA. Translation: AAC73235.1.
AP009048 Genomic DNA. Translation: BAB96699.1.
PIRiD64735. JV0107.
RefSeqiNP_414666.1. NC_000913.3.
WP_001306211.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73235; AAC73235; b0124.
BAB96699; BAB96699; BAB96699.
GeneIDi944830.
KEGGiecj:JW0120.
eco:b0124.
PATRICi32115351. VBIEscCol129921_0127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51323 Genomic DNA. Translation: CAA35706.1.
D12651 Genomic DNA. Translation: BAA02174.1.
U00096 Genomic DNA. Translation: AAC73235.1.
AP009048 Genomic DNA. Translation: BAB96699.1.
PIRiD64735. JV0107.
RefSeqiNP_414666.1. NC_000913.3.
WP_001306211.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP15877.
SMRiP15877. Positions 164-792.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261958. 8 interactions.
DIPiDIP-9747N.
IntActiP15877. 9 interactions.
MINTiMINT-1267882.
STRINGi511145.b0124.

Proteomic databases

EPDiP15877.
PaxDbiP15877.
PRIDEiP15877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73235; AAC73235; b0124.
BAB96699; BAB96699; BAB96699.
GeneIDi944830.
KEGGiecj:JW0120.
eco:b0124.
PATRICi32115351. VBIEscCol129921_0127.

Organism-specific databases

EchoBASEiEB0364.
EcoGeneiEG10369. gcd.

Phylogenomic databases

eggNOGiENOG4105DZG. Bacteria.
COG4993. LUCA.
HOGENOMiHOG000116843.
InParanoidiP15877.
KOiK00117.
OMAiDVGETTY.
PhylomeDBiP15877.

Enzyme and pathway databases

BioCyciEcoCyc:GLUCDEHYDROG-MONOMER.
ECOL316407:JW0120-MONOMER.
MetaCyc:GLUCDEHYDROG-MONOMER.
RETL1328306-WGS:GSTH-1225-MONOMER.

Miscellaneous databases

PROiP15877.

Family and domain databases

CDDicd10280. PQQ_mGDH. 1 hit.
Gene3Di2.140.10.10. 2 hits.
InterProiIPR018391. PQQ_beta_propeller_repeat.
IPR017511. PQQ_mDH.
IPR002372. PQQ_repeat.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR001479. Quinoprotein_DH_CS.
[Graphical view]
PANTHERiPTHR32303:SF4. PTHR32303:SF4. 2 hits.
PfamiPF01011. PQQ. 6 hits.
[Graphical view]
SMARTiSM00564. PQQ. 6 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 2 hits.
TIGRFAMsiTIGR03074. PQQ_membr_DH. 1 hit.
PROSITEiPS00363. BACTERIAL_PQQ_1. 1 hit.
PS00364. BACTERIAL_PQQ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHG_ECOLI
AccessioniPrimary (citable) accession number: P15877
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 167 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.