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P15873

- PCNA_YEAST

UniProt

P15873 - PCNA_YEAST

Protein

Proliferating cell nuclear antigen

Gene

POL30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi61 – 8020Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA polymerase processivity factor activity Source: SGD
    3. identical protein binding Source: IntAct
    4. protein binding Source: IntAct

    GO - Biological processi

    1. chromatin silencing at silent mating-type cassette Source: SGD
    2. chromatin silencing at telomere Source: SGD
    3. error-free translesion synthesis Source: SGD
    4. establishment of mitotic sister chromatid cohesion Source: SGD
    5. lagging strand elongation Source: SGD
    6. leading strand elongation Source: SGD
    7. maintenance of DNA trinucleotide repeats Source: SGD
    8. meiotic mismatch repair Source: SGD
    9. mismatch repair Source: SGD
    10. mitotic cell cycle Source: SGD
    11. mitotic sister chromatid cohesion Source: SGD
    12. nucleotide-excision repair Source: SGD
    13. positive regulation of exodeoxyribonuclease activity Source: SGD
    14. positive regulation of phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands Source: SGD
    15. postreplication repair Source: SGD
    16. regulation of DNA replication Source: InterPro

    Keywords - Biological processi

    DNA damage, DNA repair, DNA replication

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29055-MONOMER.
    ReactomeiREACT_189247. G1/S-Specific Transcription.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proliferating cell nuclear antigen
    Short name:
    PCNA
    Gene namesi
    Name:POL30
    Ordered Locus Names:YBR088C
    ORF Names:YBR0811
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR088c.
    SGDiS000000292. POL30.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD
    2. PCNA complex Source: SGD
    3. replication fork Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 258258Proliferating cell nuclear antigenPRO_0000149176Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki127 – 127Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
    Cross-linki164 – 164Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
    Cross-linki164 – 164Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication

    Post-translational modificationi

    Sumoylated on Lys-164, and to a lesser extent on Lys-127 by the UBC9/SIZ1 complex during S-phase; which impairs ubiquitination and function in DNA repair.3 Publications
    Monoubiquitinated on Lys-164 by the UBC2/RAD18 complex upon DNA damage, and then polyubiquitinated through 'Lys-63'-linkage by UBC13/MMS2. Ubiquitination is required for UBC2-mediated DNA repair.1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP15873.
    PaxDbiP15873.
    PeptideAtlasiP15873.

    Expressioni

    Gene expression databases

    GenevestigatoriP15873.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with RAD30 and MCM10.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-12993,EBI-12993
    ELG1Q120509EBI-12993,EBI-32195
    MCM10P323544EBI-12993,EBI-5965
    RAD27P267933EBI-12993,EBI-14693
    RAD30Q040493EBI-12993,EBI-36214
    SMT3Q123066EBI-12993,EBI-17490
    SRS2P1295410EBI-12993,EBI-18110

    Protein-protein interaction databases

    BioGridi32794. 223 interactions.
    DIPiDIP-2417N.
    IntActiP15873. 18 interactions.
    MINTiMINT-675752.
    STRINGi4932.YBR088C.

    Structurei

    Secondary structure

    1
    258
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 87
    Helixi9 – 1911
    Turni20 – 223
    Beta strandi24 – 318
    Beta strandi34 – 407
    Beta strandi44 – 5310
    Helixi54 – 563
    Beta strandi57 – 604
    Beta strandi66 – 716
    Helixi72 – 787
    Turni83 – 853
    Beta strandi86 – 927
    Beta strandi97 – 1048
    Beta strandi106 – 1094
    Beta strandi111 – 1177
    Helixi122 – 1243
    Beta strandi134 – 1407
    Helixi141 – 15212
    Beta strandi156 – 1638
    Beta strandi166 – 1727
    Beta strandi177 – 1826
    Beta strandi185 – 1873
    Beta strandi188 – 1903
    Helixi191 – 1933
    Beta strandi195 – 2017
    Beta strandi203 – 2086
    Helixi209 – 2157
    Helixi216 – 2205
    Beta strandi223 – 2297
    Beta strandi231 – 2333
    Beta strandi235 – 2417
    Beta strandi244 – 2507
    Beta strandi254 – 2563

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PLQX-ray2.30A1-258[»]
    1PLRX-ray3.00A1-258[»]
    1SXJX-ray2.85F/G/H1-258[»]
    2OD8X-ray2.80A1-258[»]
    3F1WX-ray2.90A1-258[»]
    3GPMX-ray3.80A1-258[»]
    3GPNX-ray2.50A1-258[»]
    3L0WX-ray2.80A1-163[»]
    3L0XX-ray3.00A1-163[»]
    B165-258[»]
    3L10X-ray2.80A1-163[»]
    3PGEX-ray2.80A165-258[»]
    B1-163[»]
    3V60X-ray2.60B1-258[»]
    3V61X-ray2.80B1-258[»]
    3V62X-ray2.90B/E1-258[»]
    4L60X-ray3.00A1-256[»]
    ProteinModelPortaliP15873.
    SMRiP15873. Positions 1-258.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP15873.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PCNA family.Curated

    Phylogenomic databases

    eggNOGiCOG0592.
    GeneTreeiENSGT00390000004965.
    HOGENOMiHOG000211098.
    KOiK04802.
    OMAiMKLMNLD.
    OrthoDBiEOG7M3J9S.

    Family and domain databases

    HAMAPiMF_00317. DNApol_clamp_arch.
    InterProiIPR000730. Pr_cel_nuc_antig.
    IPR022649. Pr_cel_nuc_antig_C.
    IPR022659. Pr_cel_nuc_antig_CS.
    IPR022648. Pr_cel_nuc_antig_N.
    [Graphical view]
    PANTHERiPTHR11352. PTHR11352. 1 hit.
    PfamiPF02747. PCNA_C. 1 hit.
    PF00705. PCNA_N. 1 hit.
    [Graphical view]
    PRINTSiPR00339. PCNACYCLIN.
    TIGRFAMsiTIGR00590. pcna. 1 hit.
    PROSITEiPS01251. PCNA_1. 1 hit.
    PS00293. PCNA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15873-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLEAKFEEAS LFKRIIDGFK DCVQLVNFQC KEDGIIAQAV DDSRVLLVSL    50
    EIGVEAFQEY RCDHPVTLGM DLTSLSKILR CGNNTDTLTL IADNTPDSII 100
    LLFEDTKKDR IAEYSLKLMD IDADFLKIEE LQYDSTLSLP SSEFSKIVRD 150
    LSQLSDSINI MITKETIKFV ADGDIGSGSV IIKPFVDMEH PETSIKLEMD 200
    QPVDLTFGAK YLLDIIKGSS LSDRVGIRLS SEAPALFQFD LKSGFLQFFL 250
    APKFNDEE 258
    Length:258
    Mass (Da):28,916
    Last modified:April 1, 1990 - v1
    Checksum:i9B56C5851621DDDC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16676 Genomic DNA. Translation: CAA34664.1.
    X78993 Genomic DNA. Translation: CAA55594.1.
    Z35957 Genomic DNA. Translation: CAA85038.1.
    AY557715 Genomic DNA. Translation: AAS56041.1.
    BK006936 Genomic DNA. Translation: DAA07209.1.
    PIRiS22851. WMBYET.
    RefSeqiNP_009645.1. NM_001178436.1.

    Genome annotation databases

    EnsemblFungiiYBR088C; YBR088C; YBR088C.
    GeneIDi852385.
    KEGGisce:YBR088C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16676 Genomic DNA. Translation: CAA34664.1 .
    X78993 Genomic DNA. Translation: CAA55594.1 .
    Z35957 Genomic DNA. Translation: CAA85038.1 .
    AY557715 Genomic DNA. Translation: AAS56041.1 .
    BK006936 Genomic DNA. Translation: DAA07209.1 .
    PIRi S22851. WMBYET.
    RefSeqi NP_009645.1. NM_001178436.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PLQ X-ray 2.30 A 1-258 [» ]
    1PLR X-ray 3.00 A 1-258 [» ]
    1SXJ X-ray 2.85 F/G/H 1-258 [» ]
    2OD8 X-ray 2.80 A 1-258 [» ]
    3F1W X-ray 2.90 A 1-258 [» ]
    3GPM X-ray 3.80 A 1-258 [» ]
    3GPN X-ray 2.50 A 1-258 [» ]
    3L0W X-ray 2.80 A 1-163 [» ]
    3L0X X-ray 3.00 A 1-163 [» ]
    B 165-258 [» ]
    3L10 X-ray 2.80 A 1-163 [» ]
    3PGE X-ray 2.80 A 165-258 [» ]
    B 1-163 [» ]
    3V60 X-ray 2.60 B 1-258 [» ]
    3V61 X-ray 2.80 B 1-258 [» ]
    3V62 X-ray 2.90 B/E 1-258 [» ]
    4L60 X-ray 3.00 A 1-256 [» ]
    ProteinModelPortali P15873.
    SMRi P15873. Positions 1-258.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32794. 223 interactions.
    DIPi DIP-2417N.
    IntActi P15873. 18 interactions.
    MINTi MINT-675752.
    STRINGi 4932.YBR088C.

    Proteomic databases

    MaxQBi P15873.
    PaxDbi P15873.
    PeptideAtlasi P15873.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR088C ; YBR088C ; YBR088C .
    GeneIDi 852385.
    KEGGi sce:YBR088C.

    Organism-specific databases

    CYGDi YBR088c.
    SGDi S000000292. POL30.

    Phylogenomic databases

    eggNOGi COG0592.
    GeneTreei ENSGT00390000004965.
    HOGENOMi HOG000211098.
    KOi K04802.
    OMAi MKLMNLD.
    OrthoDBi EOG7M3J9S.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29055-MONOMER.
    Reactomei REACT_189247. G1/S-Specific Transcription.

    Miscellaneous databases

    EvolutionaryTracei P15873.
    NextBioi 971192.
    PROi P15873.

    Gene expression databases

    Genevestigatori P15873.

    Family and domain databases

    HAMAPi MF_00317. DNApol_clamp_arch.
    InterProi IPR000730. Pr_cel_nuc_antig.
    IPR022649. Pr_cel_nuc_antig_C.
    IPR022659. Pr_cel_nuc_antig_CS.
    IPR022648. Pr_cel_nuc_antig_N.
    [Graphical view ]
    PANTHERi PTHR11352. PTHR11352. 1 hit.
    Pfami PF02747. PCNA_C. 1 hit.
    PF00705. PCNA_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00339. PCNACYCLIN.
    TIGRFAMsi TIGR00590. pcna. 1 hit.
    PROSITEi PS01251. PCNA_1. 1 hit.
    PS00293. PCNA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, structure and expression of the yeast proliferating cell nuclear antigen gene."
      Bauer G.A., Burgess P.M.J.
      Nucleic Acids Res. 18:261-265(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-41 AND 231-240.
    2. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
      Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
      Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Interaction with PCNA is essential for yeast DNA polymerase eta function."
      Haracska L., Kondratick C.M., Unk I., Prakash S., Prakash L.
      Mol. Cell 8:407-415(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RAD30.
    7. "RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO."
      Hoege C., Pfander B., Moldovan G.-L., Pyrowolakis G., Jentsch S.
      Nature 419:135-141(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUMOYLATION AT LYS-127 AND LYS-164, UBIQUITINATION AT LYS-164, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses."
      Zhou W., Ryan J.J., Zhou H.
      J. Biol. Chem. 279:32262-32268(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-127 AND LYS-164.
    9. "A proteomic strategy for gaining insights into protein sumoylation in yeast."
      Denison C., Rudner A.D., Gerber S.A., Bakalarski C.E., Moazed D., Gygi S.P.
      Mol. Cell. Proteomics 4:246-254(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-164.
      Strain: EJY251-11b.
    10. "Interaction between PCNA and diubiquitinated Mcm10 is essential for cell growth in budding yeast."
      Das-Bradoo S., Ricke R.M., Bielinsky A.-K.
      Mol. Cell. Biol. 26:4806-4817(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MCM10.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA."
      Krishna T.S., Kong X.P., Gary S., Burgers P.M., Kuriyan J.
      Cell 79:1233-1243(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
    13. "Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex."
      Bowman G.D., O'Donnell M., Kuriyan J.
      Nature 429:724-730(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) IN COMPLEX WITH RFC1; RCF2; RCF3; RCF4 AND RCF5.

    Entry informationi

    Entry nameiPCNA_YEAST
    AccessioniPrimary (citable) accession number: P15873
    Secondary accession number(s): D6VQ89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 141 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3