P15865 (H14_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone H1.4

Alternative name(s):
H1d

Gene names

Name:	Hist1h1e
Synonyms:	H1f4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation By similarity.
Subcellular location	Nucleus. Chromosome. Note: Mainly localizes in euchromatin By similarity.
Domain	The C-terminal domain is required for high-affinity binding to chromatin.
Post-translational modification	Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1 By similarity. H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter By similarity.
Sequence similarities	Belongs to the histone H1/H5 family. Contains 1 H15 (linker histone H1/H5 globular) domain.

Ontologies

Keywords
Cellular component	Chromosome Nucleus
Ligand	DNA-binding
PTM	Acetylation Methylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	chromosome condensation Traceable author statement PubMed 12211017. Source: RGD nucleosome assembly Inferred from electronic annotation. Source: InterPro nucleosome positioning Inferred from electronic annotation. Source: Compara
Cellular_component	chromatin Traceable author statement PubMed 12211017. Source: RGD nucleosome Inferred from electronic annotation. Source: InterPro nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 219	218	Histone H1.4		PRO_0000195924
Regions
	Domain	36 – 109	74	H15
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.3
	Modified residue	4	1	Phosphothreonine By similarity
	Modified residue	18	1	Phosphothreonine By similarity
	Modified residue	26	1	N6-acetyllysine; alternate By similarity
	Modified residue	26	1	N6-methyllysine; alternate By similarity
	Modified residue	36	1	Phosphoserine By similarity
	Modified residue	146	1	Phosphothreonine By similarity
	Modified residue	187	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	163 – 164	2	Missing in AAA41327. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P15865 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8977726B816119CC	FASTA	219	21,987
	10 20 30 40 50 60 MSETAPAAPA APAPAEKTPI KKKARKAAGG AKRKASGPPV SELITKAVAA SKERSGVSLA 70 80 90 100 110 120 ALKKALAAAG YDVEKNNSRI KLGLKSLVSK GTLVQTKGTG ASGSFKLNKK AASGEAKPKA 130 140 150 160 170 180 KKAGAAKAKK PAGAAKKPKK ATGTATPKKS TKKTPKKAKK PAAAAGAKKA KSPKKAKATK 190 200 210 AKKAPKSPAK ARAVKPKAAK PKTSKPKAAK PKKTAAKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A rat histone H2B pseudogene is closely associated with the histone H1d gene." Drabent B., Kunz C., Doenecke D. Biochim. Biophys. Acta 1172:193-196(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[2]	"Isolation of a genomic clone encoding the rat histone variant, H1d." Cole K.D., Kandala J.C., Kremer E., Kistler W.S. Gene 89:265-269(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
[3]	Bienvenut W.V., von Kriegsheim A.F., Kolch W. Submitted (AUG-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-17; 34-46 AND 55-75, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: Pheochromocytoma.
[4]	"A proposal for a coherent mammalian histone H1 nomenclature correlated with amino acid sequences." Parseghian M.H., Henschen A.H., Krieglstein K.G., Hamkalo B.A. Protein Sci. 3:575-587(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
[5]	"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY. Tissue: Renal collecting duct.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67320 Genomic DNA. Translation: CAA47734.1. M31229 Genomic DNA. Translation: AAA41327.1.
IPI	IPI00231650.
PIR	JH0159.
RefSeq	NP_579819.1. NM_133285.1.
UniGene	Rn.196361.
3D structure databases
DisProt	DP00136.
ProteinModelPortal	P15865.
SMR	P15865. Positions 36-109.
ModBase	Search...
Protein-protein interaction databases
IntAct	P15865. 1 interaction.
PTM databases
PhosphoSite	P15865.
Proteomic databases
PRIDE	P15865.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000072564; ENSRNOP00000066786; ENSRNOG00000047459.
GeneID	201097.
KEGG	rno:201097.
Organism-specific databases
CTD	3007.
Phylogenomic databases
GeneTree	ENSGT00670000097781.
HOVERGEN	HBG009035.
KO	K11275.
Gene expression databases
Genevestigator	P15865.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR005818. Histone_H1/H5. IPR005819. Histone_H5. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00538. Linker_histone. 1 hit. [Graphical view]
PRINTS	PR00624. HISTONEH5.
SMART	SM00526. H15. 1 hit. [Graphical view]
PROSITE	PS51504. H15. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	623093.

Entry information

Entry name

H14_RAT

Accession

Primary (citable) accession number: P15865

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program