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P15840

- MTSI_SPISQ

UniProt

P15840 - MTSI_SPISQ

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Protein
CPG DNA methylase
Gene
sssIM, mssSI
Organism
Spiroplasma monobiae (strain ATCC 33825 / MQ-1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This de-novo methylase methylates completely and exclusively CG residues in Spiroplasma strain MQ-1 DNA.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei141 – 1411 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
CPG DNA methylase (EC:2.1.1.37)
Alternative name(s):
Cytosine-specific methyltransferase SssI
Short name:
M.SssI
Gene namesi
Name:sssIM
Synonyms:mssSI
OrganismiSpiroplasma monobiae (strain ATCC 33825 / MQ-1)
Taxonomic identifieri2136 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesSpiroplasmataceaeSpiroplasma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386CPG DNA methylase
PRO_0000087912Add
BLAST

Interactioni

Structurei

3D structure databases

ProteinModelPortaliP15840.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 386376SAM-dependent MTase C5-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15840-1 [UniParc]FASTAAdd to Basket

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MSKVENKTKK LRVFEAFAGI GAQRKALEKV RKDEYEIVGL AEWYVPAIVM    50
YQAIHNNFHT KLEYKSVSRE EMIDYLENKT LSWNSKNPVS NGYWKRKKDD 100
ELKIIYNAIK LSEKEGNIFD IRDLYKRTLK NIDLLTYSFP CQDLSQQGIQ 150
KGMKRGSGTR SGLLWEIERA LDSTEKNDLP KYLLMENVGA LLHKKNEEEL 200
NQWKQKLESL GYQNSIEVLN AADFGSSQAR RRVFMISTLN EFVELPKGDK 250
KPKSIKKVLN KIVSEKDILN NLLKYNLTEF KKTKSNINKA SLIGYSKFNS 300
EGYVYDPEFT GPTLTASGAN SRIKIKDGSN IRKMNSDETF LYIGFDSQDG 350
KRVNEIEFLT ENQKIFVCGN SISVEVLEAI IDKIGG 386
Length:386
Mass (Da):44,313
Last modified:October 1, 1993 - v3
Checksum:iB3EB188C09E78CDF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17195 Genomic DNA. Translation: CAA35058.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17195 Genomic DNA. Translation: CAA35058.1 .

3D structure databases

ProteinModelPortali P15840.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P15840.
ChEMBLi CHEMBL4373.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, characterization, and expression in Escherichia coli of the gene coding for the CpG DNA methylase from Spiroplasma sp. strain MQ1(M.SssI)."
    Renbaum P., Abrahamove D., Fainsod A., Wilson G., Rottem S., Razin A.
    Nucleic Acids Res. 18:1145-1152(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTSI_SPISQ
AccessioniPrimary (citable) accession number: P15840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 77 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3