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P15840

- MTSI_SPISQ

UniProt

P15840 - MTSI_SPISQ

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Protein

CPG DNA methylase

Gene

sssIM

Organism
Spiroplasma monobiae (strain ATCC 33825 / MQ-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This de-novo methylase methylates completely and exclusively CG residues in Spiroplasma strain MQ-1 DNA.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei141 – 1411PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
CPG DNA methylase (EC:2.1.1.37)
Alternative name(s):
Cytosine-specific methyltransferase SssI
Short name:
M.SssI
Gene namesi
Name:sssIM
Synonyms:mssSI
OrganismiSpiroplasma monobiae (strain ATCC 33825 / MQ-1)
Taxonomic identifieri2136 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesSpiroplasmataceaeSpiroplasma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386CPG DNA methylasePRO_0000087912Add
BLAST

Interactioni

Structurei

3D structure databases

ProteinModelPortaliP15840.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 386376SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15840-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKVENKTKK LRVFEAFAGI GAQRKALEKV RKDEYEIVGL AEWYVPAIVM
60 70 80 90 100
YQAIHNNFHT KLEYKSVSRE EMIDYLENKT LSWNSKNPVS NGYWKRKKDD
110 120 130 140 150
ELKIIYNAIK LSEKEGNIFD IRDLYKRTLK NIDLLTYSFP CQDLSQQGIQ
160 170 180 190 200
KGMKRGSGTR SGLLWEIERA LDSTEKNDLP KYLLMENVGA LLHKKNEEEL
210 220 230 240 250
NQWKQKLESL GYQNSIEVLN AADFGSSQAR RRVFMISTLN EFVELPKGDK
260 270 280 290 300
KPKSIKKVLN KIVSEKDILN NLLKYNLTEF KKTKSNINKA SLIGYSKFNS
310 320 330 340 350
EGYVYDPEFT GPTLTASGAN SRIKIKDGSN IRKMNSDETF LYIGFDSQDG
360 370 380
KRVNEIEFLT ENQKIFVCGN SISVEVLEAI IDKIGG
Length:386
Mass (Da):44,313
Last modified:October 1, 1993 - v3
Checksum:iB3EB188C09E78CDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17195 Genomic DNA. Translation: CAA35058.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17195 Genomic DNA. Translation: CAA35058.1 .

3D structure databases

ProteinModelPortali P15840.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P15840.
ChEMBLi CHEMBL4373.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, characterization, and expression in Escherichia coli of the gene coding for the CpG DNA methylase from Spiroplasma sp. strain MQ1(M.SssI)."
    Renbaum P., Abrahamove D., Fainsod A., Wilson G., Rottem S., Razin A.
    Nucleic Acids Res. 18:1145-1152(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTSI_SPISQ
AccessioniPrimary (citable) accession number: P15840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1993
Last modified: October 1, 2014
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3