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P15840

- MTSI_SPISQ

UniProt

P15840 - MTSI_SPISQ

Protein

CPG DNA methylase

Gene

sssIM

Organism
Spiroplasma monobiae (strain ATCC 33825 / MQ-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 3 (01 Oct 1993)
      Previous versions | rss
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    • Comment

    Functioni

    This de-novo methylase methylates completely and exclusively CG residues in Spiroplasma strain MQ-1 DNA.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei141 – 1411PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CPG DNA methylase (EC:2.1.1.37)
    Alternative name(s):
    Cytosine-specific methyltransferase SssI
    Short name:
    M.SssI
    Gene namesi
    Name:sssIM
    Synonyms:mssSI
    OrganismiSpiroplasma monobiae (strain ATCC 33825 / MQ-1)
    Taxonomic identifieri2136 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesSpiroplasmataceaeSpiroplasma

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 386386CPG DNA methylasePRO_0000087912Add
    BLAST

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliP15840.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 386376SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15840-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKVENKTKK LRVFEAFAGI GAQRKALEKV RKDEYEIVGL AEWYVPAIVM    50
    YQAIHNNFHT KLEYKSVSRE EMIDYLENKT LSWNSKNPVS NGYWKRKKDD 100
    ELKIIYNAIK LSEKEGNIFD IRDLYKRTLK NIDLLTYSFP CQDLSQQGIQ 150
    KGMKRGSGTR SGLLWEIERA LDSTEKNDLP KYLLMENVGA LLHKKNEEEL 200
    NQWKQKLESL GYQNSIEVLN AADFGSSQAR RRVFMISTLN EFVELPKGDK 250
    KPKSIKKVLN KIVSEKDILN NLLKYNLTEF KKTKSNINKA SLIGYSKFNS 300
    EGYVYDPEFT GPTLTASGAN SRIKIKDGSN IRKMNSDETF LYIGFDSQDG 350
    KRVNEIEFLT ENQKIFVCGN SISVEVLEAI IDKIGG 386
    Length:386
    Mass (Da):44,313
    Last modified:October 1, 1993 - v3
    Checksum:iB3EB188C09E78CDF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17195 Genomic DNA. Translation: CAA35058.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17195 Genomic DNA. Translation: CAA35058.1 .

    3D structure databases

    ProteinModelPortali P15840.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P15840.
    ChEMBLi CHEMBL4373.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, characterization, and expression in Escherichia coli of the gene coding for the CpG DNA methylase from Spiroplasma sp. strain MQ1(M.SssI)."
      Renbaum P., Abrahamove D., Fainsod A., Wilson G., Rottem S., Razin A.
      Nucleic Acids Res. 18:1145-1152(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiMTSI_SPISQ
    AccessioniPrimary (citable) accession number: P15840
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 78 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3