P15840 (MTSI_SPISQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CPG DNA methylase
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase SssI
Short name=M.SssI

Gene names

Name:	sssIM
Synonyms:	mssSI

Organism

Spiroplasma sp. (strain MQ-1)

Taxonomic identifier

2136 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Entomoplasmatales › Spiroplasmataceae › Spiroplasma

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This de-novo methylase methylates completely and exclusively CG residues in Spiroplasma strain MQ-1 DNA.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	CPG DNA methylase		PRO_0000087912
Sites
	Active site	141	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15840 [UniParc]. Last modified October 1, 1993. Version 3. Checksum: B3EB188C09E78CDF	FASTA	386	44,313
	10 20 30 40 50 60 MSKVENKTKK LRVFEAFAGI GAQRKALEKV RKDEYEIVGL AEWYVPAIVM YQAIHNNFHT 70 80 90 100 110 120 KLEYKSVSRE EMIDYLENKT LSWNSKNPVS NGYWKRKKDD ELKIIYNAIK LSEKEGNIFD 130 140 150 160 170 180 IRDLYKRTLK NIDLLTYSFP CQDLSQQGIQ KGMKRGSGTR SGLLWEIERA LDSTEKNDLP 190 200 210 220 230 240 KYLLMENVGA LLHKKNEEEL NQWKQKLESL GYQNSIEVLN AADFGSSQAR RRVFMISTLN 250 260 270 280 290 300 EFVELPKGDK KPKSIKKVLN KIVSEKDILN NLLKYNLTEF KKTKSNINKA SLIGYSKFNS 310 320 330 340 350 360 EGYVYDPEFT GPTLTASGAN SRIKIKDGSN IRKMNSDETF LYIGFDSQDG KRVNEIEFLT 370 380 ENQKIFVCGN SISVEVLEAI IDKIGG

References

[1]

"Cloning, characterization, and expression in Escherichia coli of the gene coding for the CpG DNA methylase from Spiroplasma sp. strain MQ1(M.SssI)." Renbaum P., Abrahamove D., Fainsod A., Wilson G., Rottem S., Razin A. Nucleic Acids Res. 18:1145-1152(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17195 Genomic DNA. Translation: CAA35058.1.
3D structure databases
ProteinModelPortal	P15840.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P15840.
ChEMBL	CHEMBL4373.

Entry information

Entry name

MTSI_SPISQ

Accession

Primary (citable) accession number: P15840

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program