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Protein

CPG DNA methylase

Gene

sssIM

Organism
Spiroplasma monobiae (strain ATCC 33825 / MQ-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This de-novo methylase methylates completely and exclusively CG residues in Spiroplasma strain MQ-1 DNA.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei141PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
CPG DNA methylase (EC:2.1.1.37)
Alternative name(s):
Cytosine-specific methyltransferase SssI
Short name:
M.SssI
Gene namesi
Name:sssIM
Synonyms:mssSI
OrganismiSpiroplasma monobiae (strain ATCC 33825 / MQ-1)
Taxonomic identifieri2136 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesSpiroplasmataceaeSpiroplasma

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4373

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879121 – 386CPG DNA methylaseAdd BLAST386

Interactioni

Chemistry databases

BindingDBiP15840

Structurei

3D structure databases

ProteinModelPortaliP15840
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 386SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST376

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 2 hits
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P15840-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVENKTKK LRVFEAFAGI GAQRKALEKV RKDEYEIVGL AEWYVPAIVM
60 70 80 90 100
YQAIHNNFHT KLEYKSVSRE EMIDYLENKT LSWNSKNPVS NGYWKRKKDD
110 120 130 140 150
ELKIIYNAIK LSEKEGNIFD IRDLYKRTLK NIDLLTYSFP CQDLSQQGIQ
160 170 180 190 200
KGMKRGSGTR SGLLWEIERA LDSTEKNDLP KYLLMENVGA LLHKKNEEEL
210 220 230 240 250
NQWKQKLESL GYQNSIEVLN AADFGSSQAR RRVFMISTLN EFVELPKGDK
260 270 280 290 300
KPKSIKKVLN KIVSEKDILN NLLKYNLTEF KKTKSNINKA SLIGYSKFNS
310 320 330 340 350
EGYVYDPEFT GPTLTASGAN SRIKIKDGSN IRKMNSDETF LYIGFDSQDG
360 370 380
KRVNEIEFLT ENQKIFVCGN SISVEVLEAI IDKIGG
Length:386
Mass (Da):44,313
Last modified:October 1, 1993 - v3
Checksum:iB3EB188C09E78CDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17195 Genomic DNA Translation: CAA35058.1

Similar proteinsi

Entry informationi

Entry nameiMTSI_SPISQ
AccessioniPrimary (citable) accession number: P15840
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 87 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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