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Protein

Alpha-1B adrenergic receptor

Gene

Adra1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes (By similarity).By similarity

GO - Molecular functioni

  • alpha1-adrenergic receptor activity Source: RGD
  • protein heterodimerization activity Source: UniProtKB

GO - Biological processi

  • adrenergic receptor signaling pathway Source: GOC
  • negative regulation of Rho protein signal transduction Source: MGI
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of MAPK cascade Source: UniProtKB
  • regulation of cardiac muscle contraction Source: InterPro
  • regulation of vasoconstriction Source: InterPro
  • response to drug Source: RGD
  • response to estrogen Source: RGD
  • response to hormone Source: RGD
  • response to steroid hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1B adrenergic receptor
Alternative name(s):
Alpha-1B adrenoreceptor
Short name:
Alpha-1B adrenoceptor
Gene namesi
Name:Adra1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2054. Adra1b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545ExtracellularBy similarityAdd
BLAST
Transmembranei46 – 7025Helical; Name=1By similarityAdd
BLAST
Topological domaini71 – 8313CytoplasmicBy similarityAdd
BLAST
Transmembranei84 – 10522Helical; Name=2By similarityAdd
BLAST
Topological domaini106 – 11510ExtracellularBy similarity
Transmembranei116 – 14126Helical; Name=3By similarityAdd
BLAST
Topological domaini142 – 16120CytoplasmicBy similarityAdd
BLAST
Transmembranei162 – 18221Helical; Name=4By similarityAdd
BLAST
Topological domaini183 – 20119ExtracellularBy similarityAdd
BLAST
Transmembranei202 – 22423Helical; Name=5By similarityAdd
BLAST
Topological domaini225 – 29571CytoplasmicBy similarityAdd
BLAST
Transmembranei296 – 31924Helical; Name=6By similarityAdd
BLAST
Topological domaini320 – 3267ExtracellularBy similarity
Transmembranei327 – 35125Helical; Name=7By similarityAdd
BLAST
Topological domaini352 – 515164CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intercalated disc Source: RGD
  • membrane Source: RGD
  • nuclear membrane Source: UniProtKB
  • nucleus Source: UniProtKB
  • T-tubule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1907610.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Alpha-1B adrenergic receptorPRO_0000069072Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi10 – 101N-linked (GlcNAc...)Sequence analysis
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence analysis
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence analysis
Disulfide bondi118 ↔ 195PROSITE-ProRule annotation
Modified residuei264 – 2641PhosphothreonineBy similarity
Lipidationi365 – 3651S-palmitoyl cysteineSequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

PTM databases

iPTMnetiP15823.
PhosphoSiteiP15823.

Interactioni

Subunit structurei

Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes.By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-59867N.

Chemistry

BindingDBiP15823.

Structurei

3D structure databases

ProteinModelPortaliP15823.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi368 – 37811Nuclear localization signalBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi371 – 3788Poly-Arg

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG106962.
InParanoidiP15823.
PhylomeDBiP15823.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR001115. ADRA1B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF17. PTHR24248:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00556. ADRENRGCA1BR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15823-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPDLDTGHN TSAPAHWGEL KDDNFTGPNQ TSSNSTLPQL DVTRAISVGL
60 70 80 90 100
VLGAFILFAI VGNILVILSV ACNRHLRTPT NYFIVNLAIA DLLLSFTVLP
110 120 130 140 150
FSATLEVLGY WVLGRIFCDI WAAVDVLCCT ASILSLCAIS IDRYIGVRYS
160 170 180 190 200
LQYPTLVTRR KAILALLSVW VLSTVISIGP LLGWKEPAPN DDKECGVTEE
210 220 230 240 250
PFYALFSSLG SFYIPLAVIL VMYCRVYIVA KRTTKNLEAG VMKEMSNSKE
260 270 280 290 300
LTLRIHSKNF HEDTLSSTKA KGHNPRSSIA VKLFKFSREK KAAKTLGIVV
310 320 330 340 350
GMFILCWLPF FIALPLGSLF STLKPPDAVF KVVFWLGYFN SCLNPIIYPC
360 370 380 390 400
SSKEFKRAFM RILGCQCRGG RRRRRRRRLG ACAYTYRPWT RGGSLERSQS
410 420 430 440 450
RKDSLDDSGS CMSGTQRTLP SASPSPGYLG RGTQPPVELC AFPEWKPGAL
460 470 480 490 500
LSLPEPPGRR GRLDSGPLFT FKLLGDPESP GTEGDTSNGG CDTTTDLANG
510
QPGFKSNMPL APGHF
Length:515
Mass (Da):56,586
Last modified:June 1, 1994 - v2
Checksum:iAF0C3759F80C3135
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1163GRI → LSF in AAA63478 (PubMed:1706716).Curated
Sequence conflicti203 – 2031Y → C (PubMed:2156222).Curated
Sequence conflicti203 – 2031Y → C (PubMed:7989580).Curated
Sequence conflicti207 – 2071S → C (PubMed:2156222).Curated
Sequence conflicti207 – 2071S → C (PubMed:7989580).Curated
Sequence conflicti306 – 3061C → S in AAA40647 (PubMed:8406017).Curated
Sequence conflicti415 – 4162TQ → QK in CAA35934 (PubMed:2156222).Curated
Sequence conflicti440 – 4401C → Y in AAA40647 (PubMed:8406017).Curated
Sequence conflicti484 – 4863GDT → ATA in CAA35934 (PubMed:2156222).Curated
Sequence conflicti493 – 4931T → S in AAA40647 (PubMed:8406017).Curated
Sequence conflicti511 – 5111A → G in CAA35934 (PubMed:2156222).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51585 mRNA. Translation: CAA35934.1.
M60655 mRNA. Translation: AAA63478.1.
L08610, L08609 Genomic DNA. Translation: AAA40647.1.
D32045 Genomic DNA. Translation: BAA06806.1.
PIRiJC1525.
UniGeneiRn.10032.

Genome annotation databases

UCSCiRGD:2054. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51585 mRNA. Translation: CAA35934.1.
M60655 mRNA. Translation: AAA63478.1.
L08610, L08609 Genomic DNA. Translation: AAA40647.1.
D32045 Genomic DNA. Translation: BAA06806.1.
PIRiJC1525.
UniGeneiRn.10032.

3D structure databases

ProteinModelPortaliP15823.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59867N.

Chemistry

BindingDBiP15823.
ChEMBLiCHEMBL1907610.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP15823.
PhosphoSiteiP15823.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2054. rat.

Organism-specific databases

RGDi2054. Adra1b.

Phylogenomic databases

HOVERGENiHBG106962.
InParanoidiP15823.
PhylomeDBiP15823.

Miscellaneous databases

PROiP15823.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR001115. ADRA1B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF17. PTHR24248:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00556. ADRENRGCA1BR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of a rat brain cDNA encoding an alpha-1B adrenergic receptor."
    Voigt M.M., Kispert J., Chin H.
    Nucleic Acids Res. 18:1053-1053(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Molecular cloning and expression of the cDNA for the alpha 1A-adrenergic receptor. The gene for which is located on human chromosome 5."
    Lomasney J.W., Cotecchia S., Lorenz W., Leung W.-Y., Schwinn D.A., Yang-Feng T.L., Brownstein M., Lefkowitz R.J., Caron M.G.
    J. Biol. Chem. 266:6365-6369(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Isolation and characterization of the gene encoding the rat alpha 1B adrenergic receptor."
    Gao B., Kunos G.
    Gene 131:243-247(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "cAMP responsive element-mediated regulation of the gene transcription of the alpha 1B adrenergic receptor by thyrotropin."
    Kanasaki M., Matsubara H., Murasawa S., Masaki H., Nio Y., Inada M.
    J. Clin. Invest. 94:2245-2254(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-253.

Entry informationi

Entry nameiADA1B_RAT
AccessioniPrimary (citable) accession number: P15823
Secondary accession number(s): Q63215
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 1994
Last modified: May 11, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.