P15822 (ZEP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 40

Alternative name(s):
Cirhin interaction protein
Short name=CIRIP
Gate keeper of apoptosis-activating protein
Short name=GAAP
Human immunodeficiency virus type I enhancer-binding protein 1
Short name=HIV-EP1
Major histocompatibility complex-binding protein 1
Short name=MBP-1
Positive regulatory domain II-binding factor 1
Short name=PRDII-BF1

Gene names

Name:	HIVEP1
Synonyms:	ZNF40

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	2718 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV-1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, and interferon-beta genes. It may act in T-cell activation. Involved in activating HIV-1 gene expression. Isoform 2 and isoform 3 also bind to the IPCS (IRF1 and p53 common sequence) DNA sequence in the promoter region of interferon regulatory factor 1 and p53 genes and are involved in transcription regulation of these genes. Isoform 2 does not activate HIV-1 gene expression. Isoform 2 and isoform 3 may be involved in apoptosis. Ref.2 Ref.7 Ref.8 Ref.9
Subunit structure	Interacts with CIRH1A. Ref.12
Subcellular location	Isoform 1: Nucleus Ref.2 Ref.9 Ref.12. Isoform 2: Cytoplasm. Nucleus Ref.2 Ref.9 Ref.12. Isoform 3: Cytoplasm. Nucleus Ref.2 Ref.9 Ref.12.
Induction	By mitogens and phorbol ester.
Domain	Contains two sets of 2 zinc-fingers, which are widely separated and recognize the same DNA sequence. There is a fifth zinc-finger in-between.
Sequence similarities	Contains 5 C2H2-type zinc fingers.
Sequence caution	The sequence CAA35798.1 differs from that shown. Reason: Frameshift at several positions. The sequence CAH73909.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAH73982.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI14768.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI21070.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.12. Source: UniProtKB
Molecular_function	DNA binding Traceable author statement Ref.1. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATXN1	P54253	6	EBI-722264,EBI-930964
CACNA1A	O00555	2	EBI-722264,EBI-766279

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P15822-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P15822-2)

Also known as: Delta 2; GAAP-1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-2017: Missing.
     2018-2024: KWKSSLS → MGQKFQK

Isoform 3 (identifier: P15822-3)

Also known as: GAAP-2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-2002: Missing.
     2003-2024: AITTHSKSDLLVYSSKWKSSLS → MGQKFQKKSYRLVLKELRNPLL

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 2718

2718

Zinc finger protein 40

PRO_0000047369

Regions

Zinc finger

406 – 428

23

C2H2-type 1

Zinc finger

434 – 456

23

C2H2-type 2

Zinc finger

959 – 986

28

C2H2-type 3

Zinc finger

2088 – 2110

23

C2H2-type 4

Zinc finger

2116 – 2140

25

C2H2-type 5

Compositional bias

803 – 806

4

Poly-Ser

Amino acid modifications

Modified residue

429

1

Phosphothreonine Ref.11

Modified residue

492

1

Phosphoserine Ref.13

Modified residue

495

1

Phosphoserine Ref.13

Modified residue

1036

1

Phosphoserine Ref.11

Modified residue

1735

1

Phosphoserine Ref.11

Modified residue

1749

1

Phosphoserine Ref.13 Ref.14

Modified residue

1753

1

Phosphoserine Ref.13 Ref.14

Natural variations

Alternative sequence

1 – 2017

2017

Missing in isoform 2.

VSP_037714

Alternative sequence

1 – 2002

2002

Missing in isoform 3.

VSP_037715

Alternative sequence

2003 – 2024

22

AITTH…KSSLS → MGQKFQKKSYRLVLKELRNP LL in isoform 3.

VSP_037716

Alternative sequence

2018 – 2024

7

KWKSSLS → MGQKFQK in isoform 2.

VSP_037717

Natural variant

187

1

T → M.
Corresponds to variant rs2228209 [ dbSNP | Ensembl ].

VAR_057383

Natural variant

362

1

P → L.
Corresponds to variant rs34221818 [ dbSNP | Ensembl ].

VAR_057384

Natural variant

716

1

T → A.
Corresponds to variant rs2228210 [ dbSNP | Ensembl ].

VAR_057385

Natural variant

828

1

V → I.
Corresponds to variant rs2228218 [ dbSNP | Ensembl ].

VAR_057386

Natural variant

873

1

T → A. Ref.4 Ref.5
Corresponds to variant rs6900196 [ dbSNP | Ensembl ].

VAR_057387

Natural variant

1074

1

N → S. Ref.1
Corresponds to variant rs2228220 [ dbSNP | Ensembl ].

VAR_057388

Natural variant

1170

1

K → N. Ref.1
Corresponds to variant rs34258344 [ dbSNP | Ensembl ].

VAR_057389

Natural variant

1520

1

A → G. Ref.4
Corresponds to variant rs2228212 [ dbSNP | Ensembl ].

VAR_057390

Natural variant

1609

1

M → I. Ref.5
Corresponds to variant rs2228213 [ dbSNP | Ensembl ].

VAR_057391

Natural variant

1915

1

Q → R. Ref.1
Corresponds to variant rs1126472 [ dbSNP | Ensembl ].

VAR_057392

Natural variant

2444

1

T → M.
Corresponds to variant rs2228214 [ dbSNP | Ensembl ].

VAR_059892

Natural variant

2692

1

A → G. Ref.1 Ref.5
Corresponds to variant rs1042054 [ dbSNP | Ensembl ].

VAR_059893

Experimental info

Sequence conflict

515

1

P → N in CAA35798. Ref.1

Sequence conflict

1227

1

V → I in CAA35798. Ref.1

Sequence conflict

1436

1

N → G in CAA35798. Ref.1

Sequence conflict

1660

1

V → E in AAA17534. Ref.5

Sequence conflict

1883

1

I → L in CAA35798. Ref.1

Sequence conflict

2067

1

F → C in AAV85766. Ref.6

Sequence conflict

2080

1

V → I in CAA35798. Ref.1

Sequence conflict

2149

1

V → I in CAA35798. Ref.1

Sequence conflict

2388

1

S → P in AAV85766. Ref.6

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

2718

Helix Strand Turn

Details...

Turn

2091 – 2093

3

Helix

2100 – 2109

10

Beta strand

2119 – 2122

4

Beta strand

2124 – 2127

4

Helix

2128 – 2136

9

Beta strand

2137 – 2140

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 28, 2009. Version 3. Checksum: 64C1C1CF06AC25ED	FASTA	2,718	296,865
	10 20 30 40 50 60 MPRTKQIHPR NLRDKIEEAQ KELNGAEVSK KEILQAGVKG TSESLKGVKR KKIVAENHLK 70 80 90 100 110 120 KIPKSPLRNP LQAKHKQNTE ESSFAVLHSA SESHKKQNYI PVKNGKQFTK QNGETPGIIA 130 140 150 160 170 180 EASKSEESVS PKKPLFLQQP SELRRWRSEG ADPAKFSDLD EQCDSSSLSS KTRTDNSECI 190 200 210 220 230 240 SSHCGTTSPS YTNTAFDVLL KAMEPELSTL SQKGSPCAIK TEKLRPNKTA RSPPKLKNSS 250 260 270 280 290 300 MDAPNQTSQE LVAESQSSCT SYTVHMSAAQ KNEQGAMQSA SHLYHQHEHF VPKSNQHNQQ 310 320 330 340 350 360 LPGCSGFTGS LTNLQNQENA KLEQVYNIAV TSSVGLTSPS SRSQVTPQNQ QMDSASPLSI 370 380 390 400 410 420 SPANSTQSPP MPIYNSTHVA SVVNQSVEQM CNLLLKDQKP KKQGKYICEY CNRACAKPSV 430 440 450 460 470 480 LLKHIRSHTG ERPYPCVTCG FSFKTKSNLY KHKKSHAHTI KLGLVLQPDA GGLFLSHESP 490 500 510 520 530 540 KALSIHSDVE DSGESEEEGA TDERQHDLGA MELQPVHIIK RMSNAETLLK SSFTPSSPEN 550 560 570 580 590 600 VIGDFLLQDR SAESQAVTEL PKVVVHHVTV SPLRTDSPKA MDPKPELSSA QKQKDLQVTN 610 620 630 640 650 660 VQPLSANMSQ GGVSRLETNE NSHQKGDMNP LEGKQDSHVG TVHAQLQRQQ ATDYSQEQQG 670 680 690 700 710 720 KLLSPRSLGS TDSGYFSRSE SADQTVSPPT PFARRLPSTE QDSGRSNGPS AALVTTSTPS 730 740 750 760 770 780 ALPTGEKALL LPGQMRPPLA TKTLEERISK LISDNEALVD DKQLDSVKPR RTSLSRRGSI 790 800 810 820 830 840 DSPKSYIFKD SFQFDLKPVG RRTSSSSDIP KSPFTPTEKS KQVFLLSVPS LDCLPITRSN 850 860 870 880 890 900 SMPTTGYSAV PANIIPPPHP LRGSQSFDDK IGTFYDDVFV SGPNAPVPQS GHPRTLVRQA 910 920 930 940 950 960 AIEDSSANES HVLGTGQSLD ESHQGCHAAG EAMSVRSKAL AQGPHIEKKK SHQGRGTMFE 970 980 990 1000 1010 1020 CETCRNRYRK LENFENHKKF YCSELHGPKT KVAMREPEHS PVPGGLQPQI LHYRVAGSSG 1030 1040 1050 1060 1070 1080 IWEQTPQIRK RRKMKSVGDD EELQQNESGT SPKSSEGLQF QNALGCNPSL PKHNVTIRSD 1090 1100 1110 1120 1130 1140 QQHKNIQLQN SHIHLVARGP EQTMDPKLST IMEQQISSAA QDKIELQRHG TGISVIQHTN 1150 1160 1170 1180 1190 1200 SLSRPNSFDK PEPFERASPV SFQELNRTGK SGSLKVIGIS QEESHPSRDG SHPHQLALSD 1210 1220 1230 1240 1250 1260 ALRGELQESS RKSPSERHVL GQPSRLVRQH NIQVPEILVT EEPDRDLEAQ CHDQEKSEKF 1270 1280 1290 1300 1310 1320 SWPQRSETLS KLPTEKLPPK KKRLRLAEIE HSSTESSFDS TLSRSLSRES SLSHTSSFSA 1330 1340 1350 1360 1370 1380 SLDIEDVSKT EASPKIDFLN KAEFLMIPAG LNTLNVPGCH REMRRTASEQ INCTQTSMEV 1390 1400 1410 1420 1430 1440 SDLRSKSFDC GSITPPQTTP LTELQPPSSP SRVGVTGHVP LLERRRGPLV RQISLNIAPD 1450 1460 1470 1480 1490 1500 SHLSPVHPTS FQNTALPSVN AVPYQGPQLT STSLAEFSAN TLHSQTQVKD LQAETSNSSS 1510 1520 1530 1540 1550 1560 TNVFPVQQLC DINLLNQIHA PPSHQSTQLS LQVSTQGSKP DKNSVLSGSS KSEDCFAPKY 1570 1580 1590 1600 1610 1620 QLHCQVFTSG PSCSSNPVHS LPNQVISDPV GTDHCVTSAT LPTKLIDSMS NSHPLLPPEL 1630 1640 1650 1660 1670 1680 RPLGSQVQKV PSSFMLPIRL QSSVPAYCFA TLTSLPQILV TQDLPNQPIC QTNHSVVPIS 1690 1700 1710 1720 1730 1740 EEQNSVPTLQ KGHQNALPNP EKEFLCENVF SEMSQNSSLS ESLPITQKIS VGRLSPQQES 1750 1760 1770 1780 1790 1800 SASSKRMLSP ANSLDIAMEK HQKRAKDENG AVCATDVRPL EALSSRVNEA SKQKKPILVR 1810 1820 1830 1840 1850 1860 QVCTTEPLDG VMLEKDVFSQ PEISNEAVNL TNVLPADNSS TGCSKFVVIE PISELQEFEN 1870 1880 1890 1900 1910 1920 IKSSTSLTLT VRSSPAPSEN THISPLKCTD NNQERKSPGV KNQGDKVNIQ EQSQQPVTSL 1930 1940 1950 1960 1970 1980 SLFNIKDTQQ LAFPSLKTTT NFTWCYLLRQ KSLHLPQKDQ KTSAYTDWTV SASNPNPLGL 1990 2000 2010 2020 2030 2040 PTKVALALLN SKQNTGKSLY CQAITTHSKS DLLVYSSKWK SSLSKRALGN QKSTVVEFSN 2050 2060 2070 2080 2090 2100 KDASEINSEQ DKENSLIKSE PRRIKIFDGG YKSNEEYVYV RGRGRGKYIC EECGIRCKKP 2110 2120 2130 2140 2150 2160 SMLKKHIRTH TDVRPYHCTY CNFSFKTKGN LTKHMKSKAH SKKCVDLGVS VGLIDEQDTE 2170 2180 2190 2200 2210 2220 ESDEKQRFSY ERSGYDLEES DGPDEDDNEN EDDDEDSQAE SVLSATPSVT ASPQHLPSRS 2230 2240 2250 2260 2270 2280 SLQDPVSTDE DVRITDCFSG VHTDPMDVLP RALLTRMTVL STAQSDYNRK TLSPGKARQR 2290 2300 2310 2320 2330 2340 AARDENDTIP SVDTSRSPCH QMSVDYPESE EILRSSMAGK AVAITQSPSS VRLPPAAAEH 2350 2360 2370 2380 2390 2400 SPQTAAGMPS VASPHPDPQE QKQQITLQPT PGLPSPHTHL FSHLPLHSQQ QSRTPYNMVP 2410 2420 2430 2440 2450 2460 VGGIHVVPAG LTYSTFVPLQ AGPVQLTIPA VSVVHRTLGT HRNTVTEVSG TTNPAGVAEL 2470 2480 2490 2500 2510 2520 SSVVPCIPIG QIRVPGLQNL STPGLQSLPS LSMETVNIVG LANTNMAPQV HPPGLALNAV 2530 2540 2550 2560 2570 2580 GLQVLTANPS SQSSPAPQAH IPGLQILNIA LPTLIPSVSQ VAVDAQGAPE MPASQSKACE 2590 2600 2610 2620 2630 2640 TQPKQTSVAS ANQVSRTESP QGLPTVQREN AKKVLNPPAP AGDHARLDGL SKMDTEKAAS 2650 2660 2670 2680 2690 2700 ANHVKPKPEL TSIQGQPAST SQPLLKAHSE VFTKPSGQQT LSPDRQVPRP TALPRRQPTV 2710 HFSDVSSDDD EDRLVIAT
	Isoform 2 (Delta 2) (GAAP-1) [UniParc]. Checksum: 001EB17FE50AC810	FASTA	701	75,714
	10 20 30 40 50 60 MGQKFQKKRA LGNQKSTVVE FSNKDASEIN SEQDKENSLI KSEPRRIKIF DGGYKSNEEY 70 80 90 100 110 120 VYVRGRGRGK YICEECGIRC KKPSMLKKHI RTHTDVRPYH CTYCNFSFKT KGNLTKHMKS 130 140 150 160 170 180 KAHSKKCVDL GVSVGLIDEQ DTEESDEKQR FSYERSGYDL EESDGPDEDD NENEDDDEDS 190 200 210 220 230 240 QAESVLSATP SVTASPQHLP SRSSLQDPVS TDEDVRITDC FSGVHTDPMD VLPRALLTRM 250 260 270 280 290 300 TVLSTAQSDY NRKTLSPGKA RQRAARDEND TIPSVDTSRS PCHQMSVDYP ESEEILRSSM 310 320 330 340 350 360 AGKAVAITQS PSSVRLPPAA AEHSPQTAAG MPSVASPHPD PQEQKQQITL QPTPGLPSPH 370 380 390 400 410 420 THLFSHLPLH SQQQSRTPYN MVPVGGIHVV PAGLTYSTFV PLQAGPVQLT IPAVSVVHRT 430 440 450 460 470 480 LGTHRNTVTE VSGTTNPAGV AELSSVVPCI PIGQIRVPGL QNLSTPGLQS LPSLSMETVN 490 500 510 520 530 540 IVGLANTNMA PQVHPPGLAL NAVGLQVLTA NPSSQSSPAP QAHIPGLQIL NIALPTLIPS 550 560 570 580 590 600 VSQVAVDAQG APEMPASQSK ACETQPKQTS VASANQVSRT ESPQGLPTVQ RENAKKVLNP 610 620 630 640 650 660 PAPAGDHARL DGLSKMDTEK AASANHVKPK PELTSIQGQP ASTSQPLLKA HSEVFTKPSG 670 680 690 700 QQTLSPDRQV PRPTALPRRQ PTVHFSDVSS DDDEDRLVIA T
	Isoform 3 (GAAP-2) [UniParc]. Checksum: 1F340537E16FCFF1	FASTA	716	77,538
	10 20 30 40 50 60 MGQKFQKKSY RLVLKELRNP LLKRALGNQK STVVEFSNKD ASEINSEQDK ENSLIKSEPR 70 80 90 100 110 120 RIKIFDGGYK SNEEYVYVRG RGRGKYICEE CGIRCKKPSM LKKHIRTHTD VRPYHCTYCN 130 140 150 160 170 180 FSFKTKGNLT KHMKSKAHSK KCVDLGVSVG LIDEQDTEES DEKQRFSYER SGYDLEESDG 190 200 210 220 230 240 PDEDDNENED DDEDSQAESV LSATPSVTAS PQHLPSRSSL QDPVSTDEDV RITDCFSGVH 250 260 270 280 290 300 TDPMDVLPRA LLTRMTVLST AQSDYNRKTL SPGKARQRAA RDENDTIPSV DTSRSPCHQM 310 320 330 340 350 360 SVDYPESEEI LRSSMAGKAV AITQSPSSVR LPPAAAEHSP QTAAGMPSVA SPHPDPQEQK 370 380 390 400 410 420 QQITLQPTPG LPSPHTHLFS HLPLHSQQQS RTPYNMVPVG GIHVVPAGLT YSTFVPLQAG 430 440 450 460 470 480 PVQLTIPAVS VVHRTLGTHR NTVTEVSGTT NPAGVAELSS VVPCIPIGQI RVPGLQNLST 490 500 510 520 530 540 PGLQSLPSLS METVNIVGLA NTNMAPQVHP PGLALNAVGL QVLTANPSSQ SSPAPQAHIP 550 560 570 580 590 600 GLQILNIALP TLIPSVSQVA VDAQGAPEMP ASQSKACETQ PKQTSVASAN QVSRTESPQG 610 620 630 640 650 660 LPTVQRENAK KVLNPPAPAG DHARLDGLSK MDTEKAASAN HVKPKPELTS IQGQPASTSQ 670 680 690 700 710 PLLKAHSEVF TKPSGQQTLS PDRQVPRPTA LPRRQPTVHF SDVSSDDDED RLVIAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A DNA-binding protein containing two widely separated zinc finger motifs that recognize the same DNA sequence." Fan C.M., Maniatis T. Genes Dev. 4:29-42(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS SER-1074; ASN-1170; ARG-1915 AND GLY-2692.
[2]	"Transcriptional regulator of genes involved in the control of cell growth or cell proliferation. Use of said regulator as a therapeutic or diagnostic agent." Tovey M. Patent number CA2448384, 12-DEC-2002 Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), FUNCTION (ISOFORMS 2 AND 3), SUBCELLULAR LOCATION (ISOFORMS 2 AND 3).
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ALA-873 AND GLY-1520.
[5]	"A large protein containing zinc finger domains binds to related sequence elements in the enhancers of the class I major histocompatibility complex and kappa immunoglobulin genes." Baldwin A.S. Jr., LeClair K.P., Singh H., Sharp P.A. Mol. Cell. Biol. 10:1406-1414(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 817-2718 (ISOFORM 1), VARIANTS ALA-873; ILE-1609 AND GLY-2692.
[6]	Yu B., Mitchell G.A., Richter A. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2035-2718 (ISOFORM 1). Tissue: Liver.
[7]	"Regulation of human immunodeficiency virus enhancer function by PRDII-BF1 and c-rel gene products." Muchardt C., Seeler J.S., Nirula A., Shurland D.L., Gaynor R.B. J. Virol. 66:244-250(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION (ISOFORM 2), ALTERNATIVE SPLICING.
[8]	"Transcription factor PRDII-BF1 activates human immunodeficiency virus type 1 gene expression." Seeler J.S., Muchardt C., Suessle A., Gaynor R.B. J. Virol. 68:1002-1009(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION (ISOFORM 1).
[9]	"GAAP-1: a transcriptional activator of p53 and IRF-1 possesses pro-apoptotic activity." Lallemand C., Palmieri M., Blanchard B., Meritet J.F., Tovey M.G. EMBO Rep. 3:153-158(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION (ISOFORM 2), SUBCELLULAR LOCATION (ISOFORM 2), ALTERNATIVE SPLICING.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-429; SER-1036 AND SER-1735, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Cirhin up-regulates a canonical NF-kappaB element through strong interaction with Cirip/HIVEP1." Yu B., Mitchell G.A., Richter A. Exp. Cell Res. 315:3086-3098(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CIRH1A.
[13]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-492; SER-495; SER-1749 AND SER-1753, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[14]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1749 AND SER-1753, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"High-resolution three-dimensional structure of a single zinc finger from a human enhancer binding protein in solution." Omichinski J.G., Clore G.M., Appella E., Sakaguchi K., Gronenborn A.M. Biochemistry 29:9324-9334(1990) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 2114-2143.
[16]	"High-resolution solution structure of the double Cys2His2 zinc finger from the human enhancer binding protein MBP-1." Omichinski J.G., Clore G.M., Robien M., Sakaguchi K., Appella E., Gronenborn A.M. Biochemistry 31:3907-3917(1992) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 2088-2143.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X51435 mRNA. Translation: CAA35798.1. Frameshift.
AL391828 , AL137221, AL157373, Z98050 Genomic DNA. Translation: CAH73909.1. Sequence problems.
AL157373 , AL137221, AL391828, Z98050 Genomic DNA. Translation: CAH73982.1. Sequence problems.
AL137221 , AL157373, AL391828, Z98050 Genomic DNA. Translation: CAI14768.1. Sequence problems.
Z98050 , AL137221, AL157373, AL391828 Genomic DNA. Translation: CAI21070.1. Sequence problems.
BC140816 mRNA. Translation: AAI40817.1.
M32019 mRNA. Translation: AAA17534.1.
AY673640 mRNA. Translation: AAV85766.1.

IPI

IPI00783879.
IPI00941780.
IPI00942123.

PIR

A34203.

RefSeq

NP_002105.2. NM_002114.2.

UniGene

Hs.567284.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BBO	NMR	-	A	2087-2143	[»]
3ZNF	NMR	-	A	2114-2143	[»]
4ZNF	NMR	-	A	2114-2143	[»]

ProteinModelPortal

P15822.

ModBase

Search...

Protein-protein interaction databases

IntAct

P15822. 54 interactions.

MINT

MINT-1188428.

STRING

9606.ENSP00000368698.

PTM databases

PhosphoSite

P15822.

Polymorphism databases

DMDM

254763385.

Proteomic databases

PaxDb

P15822.

PRIDE

P15822.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000379388; ENSP00000368698; ENSG00000095951.

GeneID

3096.

KEGG

hsa:3096.

UCSC

uc003nac.3. human.

Organism-specific databases

CTD

3096.

GeneCards

GC06P012009.

HGNC

HGNC:4920. HIVEP1.

HPA

HPA050724.

MIM

194540. gene.

neXtProt

NX_P15822.

PharmGKB

PA29297.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG296349.

HOGENOM

HOG000169307.

HOVERGEN

HBG018160.

KO

K09239.

OMA

PPLATKT.

OrthoDB

EOG4DNF3H.

Gene expression databases

ArrayExpress

P15822.

Bgee

P15822.

CleanEx

HS_HIVEP1.

Genevestigator

P15822.

GermOnline

ENSG00000095951. Homo sapiens.

Family and domain databases

Gene3D

3.30.160.60. 4 hits.

InterPro

IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 5 hits.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL2909.

ChiTaRS

HIVEP1. human.

EvolutionaryTrace

P15822.

GenomeRNAi

3096.

NextBio

12285.

SOURCE

Search...

Entry information

Entry name

ZEP1_HUMAN

Accession

Primary (citable) accession number: P15822
Secondary accession number(s): B2RTU3 , Q14122, Q5MPB1, Q5VW60

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 140 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.