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Protein

Siroheme biosynthesis protein MET8

Gene

MET8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of precorrin-2 into siroheme. This reaction consist of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme.1 Publication

Catalytic activityi

Precorrin-2 + NAD+ = sirohydrochlorin + NADH.
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme biosynthesis protein MET8 (MET8)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme biosynthesis protein MET8 (MET8)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93NAD; via amide nitrogen1 Publication1
Active sitei141Proton acceptorSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 24NAD1 Publication2
Nucleotide bindingi43 – 45NAD1 Publication3

GO - Molecular functioni

GO - Biological processi

  • methionine biosynthetic process Source: UniProtKB-KW
  • one-carbon metabolic process Source: GO_Central
  • siroheme biosynthetic process Source: SGD
  • sulfate assimilation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:G3O-29150-MONOMER.
YEAST:G3O-29150-MONOMER.
BRENDAi1.3.1.76. 984.
4.99.1.4. 984.
UniPathwayiUPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme biosynthesis protein MET8
Including the following 2 domains:
Precorrin-2 dehydrogenase (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase (EC:4.99.1.4)
Gene namesi
Name:MET8
Ordered Locus Names:YBR213W
ORF Names:YBR1461
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR213W.
SGDiS000000417. MET8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi22G → D: Loss of dehydrogenase activity. No effect on chelatase activity. 1 Publication1
Mutagenesisi141D → A: Loss of chelatase and dehydrogenase activity. 1 Publication1
Mutagenesisi237H → A: No effect on dehydrogenase or chelatase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000964461 – 274Siroheme biosynthesis protein MET8Add BLAST274

Proteomic databases

MaxQBiP15807.
PRIDEiP15807.

PTM databases

iPTMnetiP15807.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi32910. 20 interactors.
DIPiDIP-4944N.
IntActiP15807. 1 interactor.
MINTiMINT-553462.

Structurei

Secondary structure

1274
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Beta strandi15 – 22Combined sources8
Helixi23 – 32Combined sources10
Helixi33 – 35Combined sources3
Beta strandi38 – 46Combined sources9
Helixi50 – 54Combined sources5
Helixi56 – 58Combined sources3
Turni68 – 70Combined sources3
Beta strandi72 – 74Combined sources3
Beta strandi86 – 89Combined sources4
Helixi95 – 98Combined sources4
Beta strandi107 – 112Combined sources6
Helixi117 – 131Combined sources15
Beta strandi135 – 139Combined sources5
Helixi143 – 145Combined sources3
Beta strandi146 – 149Combined sources4
Beta strandi152 – 156Combined sources5
Turni157 – 159Combined sources3
Beta strandi160 – 169Combined sources10
Helixi171 – 188Combined sources18
Helixi193 – 210Combined sources18
Helixi214 – 216Combined sources3
Helixi217 – 235Combined sources19
Helixi236 – 238Combined sources3
Helixi241 – 252Combined sources12
Turni253 – 256Combined sources4
Helixi264 – 270Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KYQX-ray2.20A/B/C1-274[»]
ProteinModelPortaliP15807.
SMRiP15807.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15807.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000181024.
InParanoidiP15807.
KOiK02304.
OMAiVCCCIDD.
OrthoDBiEOG092C3NWX.

Family and domain databases

Gene3Di1.10.3280.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR028161. Met8.
IPR028162. Met8_C.
IPR016040. NAD(P)-bd_dom.
IPR028281. Sirohaem_synthase_central.
IPR006367. Sirohaem_synthase_N.
[Graphical view]
PANTHERiPTHR21091:SF106. PTHR21091:SF106. 1 hit.
PfamiPF13241. NAD_binding_7. 1 hit.
PF14823. Sirohm_synth_C. 1 hit.
PF14824. Sirohm_synth_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P15807-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKSLQLAHQ LKDKKILLIG GGEVGLTRLY KLIPTGCKLT LVSPDLHKSI
60 70 80 90 100
IPKFGKFIQN EDQPDYREDA KRFINPNWDP TKNEIYEYIR SDFKDEYLDL
110 120 130 140 150
EDENDAWYII MTCIPDHPES ARIYHLCKER FGKQQLVNVA DKPDLCDFYF
160 170 180 190 200
GANLEIGDRL QILISTNGLS PRFGALVRDE IRNLFTQMGD LALEDAVVKL
210 220 230 240 250
GELRRGIRLL APDDKDVKYR MDWARRCTDL FGIQHCHNID VKRLLDLFKV
260 270
MFQEQNCSLQ FPPRERLLSE YCSS
Length:274
Mass (Da):31,918
Last modified:April 1, 1990 - v1
Checksum:i0EE9A6DE8A43673E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15K → R (PubMed:11980703).Curated1
Sequence conflicti33I → M (PubMed:11980703).Curated1
Sequence conflicti61E → K (PubMed:11980703).Curated1
Sequence conflicti102D → N (PubMed:11980703).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17271 Genomic DNA. Translation: CAA35173.1.
Z36082 Genomic DNA. Translation: CAA85177.1.
BK006936 Genomic DNA. Translation: DAA07329.1.
PIRiS20155.
RefSeqiNP_009772.1. NM_001178561.1.

Genome annotation databases

EnsemblFungiiYBR213W; YBR213W; YBR213W.
GeneIDi852514.
KEGGisce:YBR213W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17271 Genomic DNA. Translation: CAA35173.1.
Z36082 Genomic DNA. Translation: CAA85177.1.
BK006936 Genomic DNA. Translation: DAA07329.1.
PIRiS20155.
RefSeqiNP_009772.1. NM_001178561.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KYQX-ray2.20A/B/C1-274[»]
ProteinModelPortaliP15807.
SMRiP15807.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32910. 20 interactors.
DIPiDIP-4944N.
IntActiP15807. 1 interactor.
MINTiMINT-553462.

PTM databases

iPTMnetiP15807.

Proteomic databases

MaxQBiP15807.
PRIDEiP15807.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR213W; YBR213W; YBR213W.
GeneIDi852514.
KEGGisce:YBR213W.

Organism-specific databases

EuPathDBiFungiDB:YBR213W.
SGDiS000000417. MET8.

Phylogenomic databases

HOGENOMiHOG000181024.
InParanoidiP15807.
KOiK02304.
OMAiVCCCIDD.
OrthoDBiEOG092C3NWX.

Enzyme and pathway databases

UniPathwayiUPA00262; UER00222.
UPA00262; UER00376.
BioCyciMetaCyc:G3O-29150-MONOMER.
YEAST:G3O-29150-MONOMER.
BRENDAi1.3.1.76. 984.
4.99.1.4. 984.

Miscellaneous databases

EvolutionaryTraceiP15807.
PROiP15807.

Family and domain databases

Gene3Di1.10.3280.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR028161. Met8.
IPR028162. Met8_C.
IPR016040. NAD(P)-bd_dom.
IPR028281. Sirohaem_synthase_central.
IPR006367. Sirohaem_synthase_N.
[Graphical view]
PANTHERiPTHR21091:SF106. PTHR21091:SF106. 1 hit.
PfamiPF13241. NAD_binding_7. 1 hit.
PF14823. Sirohm_synth_C. 1 hit.
PF14824. Sirohm_synth_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMET8_YEAST
AccessioniPrimary (citable) accession number: P15807
Secondary accession number(s): D6VQK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 2, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.