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P15807

- MET8_YEAST

UniProt

P15807 - MET8_YEAST

Protein

Siroheme biosynthesis protein MET8

Gene

MET8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of precorrin-2 into siroheme. This reaction consist of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme.1 Publication

    Catalytic activityi

    Precorrin-2 + NAD+ = sirohydrochlorin + NADH.
    Siroheme + 2 H+ = sirohydrochlorin + Fe2+.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei93 – 931NAD; via amide nitrogen1 Publication
    Active sitei141 – 1411Proton acceptorSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi23 – 242NAD1 Publication
    Nucleotide bindingi43 – 453NAD1 Publication

    GO - Molecular functioni

    1. ferrochelatase activity Source: SGD
    2. precorrin-2 dehydrogenase activity Source: SGD
    3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine biosynthetic process Source: UniProtKB-KW
    2. siroheme biosynthetic process Source: SGD
    3. sulfate assimilation Source: SGD

    Keywords - Molecular functioni

    Lyase, Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-29150-MONOMER.
    YEAST:G3O-29150-MONOMER.
    BRENDAi4.99.1.4. 984.
    UniPathwayiUPA00262; UER00222.
    UPA00262; UER00376.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Siroheme biosynthesis protein MET8
    Including the following 2 domains:
    Precorrin-2 dehydrogenase (EC:1.3.1.76)
    Sirohydrochlorin ferrochelatase (EC:4.99.1.4)
    Gene namesi
    Name:MET8
    Ordered Locus Names:YBR213W
    ORF Names:YBR1461
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR213w.
    SGDiS000000417. MET8.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi22 – 221G → D: Loss of dehydrogenase activity. No effect on chelatase activity. 1 Publication
    Mutagenesisi141 – 1411D → A: Loss of chelatase and dehydrogenase activity. 1 Publication
    Mutagenesisi237 – 2371H → A: No effect on dehydrogenase or chelatase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 274274Siroheme biosynthesis protein MET8PRO_0000096446Add
    BLAST

    Proteomic databases

    MaxQBiP15807.
    PaxDbiP15807.
    PRIDEiP15807.

    Expressioni

    Gene expression databases

    GenevestigatoriP15807.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi32910. 16 interactions.
    DIPiDIP-4944N.
    MINTiMINT-553462.

    Structurei

    Secondary structure

    1
    274
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 95
    Beta strandi15 – 228
    Helixi23 – 3210
    Helixi33 – 353
    Beta strandi38 – 469
    Helixi50 – 545
    Helixi56 – 583
    Beta strandi72 – 743
    Beta strandi86 – 894
    Helixi95 – 984
    Beta strandi107 – 1126
    Helixi117 – 13115
    Beta strandi135 – 1395
    Helixi143 – 1453
    Beta strandi146 – 1494
    Beta strandi152 – 1565
    Turni157 – 1593
    Beta strandi160 – 16910
    Helixi171 – 18818
    Helixi193 – 21018
    Helixi214 – 2163
    Helixi217 – 23519
    Helixi236 – 2383
    Helixi241 – 25212
    Turni253 – 2564
    Helixi264 – 2707

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KYQX-ray2.20A/B/C1-274[»]
    ProteinModelPortaliP15807.
    SMRiP15807. Positions 1-273.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP15807.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1648.
    HOGENOMiHOG000181024.
    KOiK02304.
    OMAiVCCCIDD.
    OrthoDBiEOG7WHHP1.

    Family and domain databases

    Gene3Di1.10.3280.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR028161. Met8.
    IPR028162. Met8_C.
    IPR016040. NAD(P)-bd_dom.
    IPR028281. Sirohaem_synthase_central.
    IPR006367. Sirohaem_synthase_N.
    [Graphical view]
    PANTHERiPTHR21091:SF88. PTHR21091:SF88. 1 hit.
    PfamiPF13241. NAD_binding_7. 1 hit.
    PF14823. Sirohm_synth_C. 1 hit.
    PF14824. Sirohm_synth_M. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15807-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKSLQLAHQ LKDKKILLIG GGEVGLTRLY KLIPTGCKLT LVSPDLHKSI    50
    IPKFGKFIQN EDQPDYREDA KRFINPNWDP TKNEIYEYIR SDFKDEYLDL 100
    EDENDAWYII MTCIPDHPES ARIYHLCKER FGKQQLVNVA DKPDLCDFYF 150
    GANLEIGDRL QILISTNGLS PRFGALVRDE IRNLFTQMGD LALEDAVVKL 200
    GELRRGIRLL APDDKDVKYR MDWARRCTDL FGIQHCHNID VKRLLDLFKV 250
    MFQEQNCSLQ FPPRERLLSE YCSS 274
    Length:274
    Mass (Da):31,918
    Last modified:April 1, 1990 - v1
    Checksum:i0EE9A6DE8A43673E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti15 – 151K → R(PubMed:11980703)Curated
    Sequence conflicti33 – 331I → M(PubMed:11980703)Curated
    Sequence conflicti61 – 611E → K(PubMed:11980703)Curated
    Sequence conflicti102 – 1021D → N(PubMed:11980703)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17271 Genomic DNA. Translation: CAA35173.1.
    Z36082 Genomic DNA. Translation: CAA85177.1.
    BK006936 Genomic DNA. Translation: DAA07329.1.
    PIRiS20155.
    RefSeqiNP_009772.1. NM_001178561.1.

    Genome annotation databases

    EnsemblFungiiYBR213W; YBR213W; YBR213W.
    GeneIDi852514.
    KEGGisce:YBR213W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17271 Genomic DNA. Translation: CAA35173.1 .
    Z36082 Genomic DNA. Translation: CAA85177.1 .
    BK006936 Genomic DNA. Translation: DAA07329.1 .
    PIRi S20155.
    RefSeqi NP_009772.1. NM_001178561.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KYQ X-ray 2.20 A/B/C 1-274 [» ]
    ProteinModelPortali P15807.
    SMRi P15807. Positions 1-273.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32910. 16 interactions.
    DIPi DIP-4944N.
    MINTi MINT-553462.

    Proteomic databases

    MaxQBi P15807.
    PaxDbi P15807.
    PRIDEi P15807.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR213W ; YBR213W ; YBR213W .
    GeneIDi 852514.
    KEGGi sce:YBR213W.

    Organism-specific databases

    CYGDi YBR213w.
    SGDi S000000417. MET8.

    Phylogenomic databases

    eggNOGi COG1648.
    HOGENOMi HOG000181024.
    KOi K02304.
    OMAi VCCCIDD.
    OrthoDBi EOG7WHHP1.

    Enzyme and pathway databases

    UniPathwayi UPA00262 ; UER00222 .
    UPA00262 ; UER00376 .
    BioCyci MetaCyc:G3O-29150-MONOMER.
    YEAST:G3O-29150-MONOMER.
    BRENDAi 4.99.1.4. 984.

    Miscellaneous databases

    EvolutionaryTracei P15807.
    NextBioi 971538.

    Gene expression databases

    Genevestigatori P15807.

    Family and domain databases

    Gene3Di 1.10.3280.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR028161. Met8.
    IPR028162. Met8_C.
    IPR016040. NAD(P)-bd_dom.
    IPR028281. Sirohaem_synthase_central.
    IPR006367. Sirohaem_synthase_N.
    [Graphical view ]
    PANTHERi PTHR21091:SF88. PTHR21091:SF88. 1 hit.
    Pfami PF13241. NAD_binding_7. 1 hit.
    PF14823. Sirohm_synth_C. 1 hit.
    PF14824. Sirohm_synth_M. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the MET8 gene of Saccharomyces cerevisiae."
      Cherest H., Thomas D., Surdin-Kerjan Y.
      Nucleic Acids Res. 18:659-659(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 26786 / X2180-1A.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The role of Saccharomyces cerevisiae Met1p and Met8p in sirohaem and cobalamin biosynthesis."
      Raux E., McVeigh T., Peters S.E., Leustek T., Warren M.J.
      Biochem. J. 338:701-708(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase."
      Schubert H.L., Raux E., Brindley A.A., Leech H.K., Wilson K.S., Hill C.P., Warren M.J.
      EMBO J. 21:2068-2075(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 11-274 IN COMPLEX WITH NAD, SUBUNIT, MUTAGENESIS OF GLY-22; ASP-141 AND HIS-237.

    Entry informationi

    Entry nameiMET8_YEAST
    AccessioniPrimary (citable) accession number: P15807
    Secondary accession number(s): D6VQK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3