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P15807

- MET8_YEAST

UniProt

P15807 - MET8_YEAST

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Protein
Siroheme biosynthesis protein MET8
Gene
MET8, YBR213W, YBR1461
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of precorrin-2 into siroheme. This reaction consist of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme.1 Publication

Catalytic activityi

Precorrin-2 + NAD+ = sirohydrochlorin + NADH.
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931NAD; via amide nitrogen
Active sitei141 – 1411Proton acceptor Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi23 – 242NAD
Nucleotide bindingi43 – 453NAD

GO - Molecular functioni

  1. ferrochelatase activity Source: SGD
  2. precorrin-2 dehydrogenase activity Source: SGD
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. siroheme biosynthetic process Source: SGD
  3. sulfate assimilation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:G3O-29150-MONOMER.
YEAST:G3O-29150-MONOMER.
BRENDAi4.99.1.4. 984.
UniPathwayiUPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme biosynthesis protein MET8
Including the following 2 domains:
Precorrin-2 dehydrogenase (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase (EC:4.99.1.4)
Gene namesi
Name:MET8
Ordered Locus Names:YBR213W
ORF Names:YBR1461
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR213w.
SGDiS000000417. MET8.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi22 – 221G → D: Loss of dehydrogenase activity. No effect on chelatase activity. 1 Publication
Mutagenesisi141 – 1411D → A: Loss of chelatase and dehydrogenase activity. 1 Publication
Mutagenesisi237 – 2371H → A: No effect on dehydrogenase or chelatase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Siroheme biosynthesis protein MET8
PRO_0000096446Add
BLAST

Proteomic databases

MaxQBiP15807.
PaxDbiP15807.
PRIDEiP15807.

Expressioni

Gene expression databases

GenevestigatoriP15807.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi32910. 16 interactions.
DIPiDIP-4944N.
MINTiMINT-553462.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95
Beta strandi15 – 228
Helixi23 – 3210
Helixi33 – 353
Beta strandi38 – 469
Helixi50 – 545
Helixi56 – 583
Beta strandi72 – 743
Beta strandi86 – 894
Helixi95 – 984
Beta strandi107 – 1126
Helixi117 – 13115
Beta strandi135 – 1395
Helixi143 – 1453
Beta strandi146 – 1494
Beta strandi152 – 1565
Turni157 – 1593
Beta strandi160 – 16910
Helixi171 – 18818
Helixi193 – 21018
Helixi214 – 2163
Helixi217 – 23519
Helixi236 – 2383
Helixi241 – 25212
Turni253 – 2564
Helixi264 – 2707

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KYQX-ray2.20A/B/C1-274[»]
ProteinModelPortaliP15807.
SMRiP15807. Positions 1-273.

Miscellaneous databases

EvolutionaryTraceiP15807.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1648.
HOGENOMiHOG000181024.
KOiK02304.
OMAiVCCCIDD.
OrthoDBiEOG7WHHP1.

Family and domain databases

Gene3Di1.10.3280.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR028161. Met8.
IPR028162. Met8_C.
IPR016040. NAD(P)-bd_dom.
IPR028281. Sirohaem_synthase_central.
IPR006367. Sirohaem_synthase_N.
[Graphical view]
PANTHERiPTHR21091:SF88. PTHR21091:SF88. 1 hit.
PfamiPF13241. NAD_binding_7. 1 hit.
PF14823. Sirohm_synth_C. 1 hit.
PF14824. Sirohm_synth_M. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15807-1 [UniParc]FASTAAdd to Basket

« Hide

MVKSLQLAHQ LKDKKILLIG GGEVGLTRLY KLIPTGCKLT LVSPDLHKSI    50
IPKFGKFIQN EDQPDYREDA KRFINPNWDP TKNEIYEYIR SDFKDEYLDL 100
EDENDAWYII MTCIPDHPES ARIYHLCKER FGKQQLVNVA DKPDLCDFYF 150
GANLEIGDRL QILISTNGLS PRFGALVRDE IRNLFTQMGD LALEDAVVKL 200
GELRRGIRLL APDDKDVKYR MDWARRCTDL FGIQHCHNID VKRLLDLFKV 250
MFQEQNCSLQ FPPRERLLSE YCSS 274
Length:274
Mass (Da):31,918
Last modified:April 1, 1990 - v1
Checksum:i0EE9A6DE8A43673E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151K → R1 Publication
Sequence conflicti33 – 331I → M1 Publication
Sequence conflicti61 – 611E → K1 Publication
Sequence conflicti102 – 1021D → N1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17271 Genomic DNA. Translation: CAA35173.1.
Z36082 Genomic DNA. Translation: CAA85177.1.
BK006936 Genomic DNA. Translation: DAA07329.1.
PIRiS20155.
RefSeqiNP_009772.1. NM_001178561.1.

Genome annotation databases

EnsemblFungiiYBR213W; YBR213W; YBR213W.
GeneIDi852514.
KEGGisce:YBR213W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17271 Genomic DNA. Translation: CAA35173.1 .
Z36082 Genomic DNA. Translation: CAA85177.1 .
BK006936 Genomic DNA. Translation: DAA07329.1 .
PIRi S20155.
RefSeqi NP_009772.1. NM_001178561.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KYQ X-ray 2.20 A/B/C 1-274 [» ]
ProteinModelPortali P15807.
SMRi P15807. Positions 1-273.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32910. 16 interactions.
DIPi DIP-4944N.
MINTi MINT-553462.

Proteomic databases

MaxQBi P15807.
PaxDbi P15807.
PRIDEi P15807.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR213W ; YBR213W ; YBR213W .
GeneIDi 852514.
KEGGi sce:YBR213W.

Organism-specific databases

CYGDi YBR213w.
SGDi S000000417. MET8.

Phylogenomic databases

eggNOGi COG1648.
HOGENOMi HOG000181024.
KOi K02304.
OMAi VCCCIDD.
OrthoDBi EOG7WHHP1.

Enzyme and pathway databases

UniPathwayi UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci MetaCyc:G3O-29150-MONOMER.
YEAST:G3O-29150-MONOMER.
BRENDAi 4.99.1.4. 984.

Miscellaneous databases

EvolutionaryTracei P15807.
NextBioi 971538.

Gene expression databases

Genevestigatori P15807.

Family and domain databases

Gene3Di 1.10.3280.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR028161. Met8.
IPR028162. Met8_C.
IPR016040. NAD(P)-bd_dom.
IPR028281. Sirohaem_synthase_central.
IPR006367. Sirohaem_synthase_N.
[Graphical view ]
PANTHERi PTHR21091:SF88. PTHR21091:SF88. 1 hit.
Pfami PF13241. NAD_binding_7. 1 hit.
PF14823. Sirohm_synth_C. 1 hit.
PF14824. Sirohm_synth_M. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the MET8 gene of Saccharomyces cerevisiae."
    Cherest H., Thomas D., Surdin-Kerjan Y.
    Nucleic Acids Res. 18:659-659(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 26786 / X2180-1A.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The role of Saccharomyces cerevisiae Met1p and Met8p in sirohaem and cobalamin biosynthesis."
    Raux E., McVeigh T., Peters S.E., Leustek T., Warren M.J.
    Biochem. J. 338:701-708(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase."
    Schubert H.L., Raux E., Brindley A.A., Leech H.K., Wilson K.S., Hill C.P., Warren M.J.
    EMBO J. 21:2068-2075(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 11-274 IN COMPLEX WITH NAD, SUBUNIT, MUTAGENESIS OF GLY-22; ASP-141 AND HIS-237.

Entry informationi

Entry nameiMET8_YEAST
AccessioniPrimary (citable) accession number: P15807
Secondary accession number(s): D6VQK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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