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P15807 (MET8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Siroheme biosynthesis protein MET8

Including the following 2 domains:

  1. Precorrin-2 dehydrogenase
    EC=1.3.1.76
  2. Sirohydrochlorin ferrochelatase
    EC=4.99.1.4
Gene names
Name:MET8
Ordered Locus Names:YBR213W
ORF Names:YBR1461
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of precorrin-2 into siroheme. This reaction consist of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme. Ref.4

Catalytic activity

Precorrin-2 + NAD+ = sirohydrochlorin + NADH.

Siroheme + 2 H+ = sirohydrochlorin + Fe2+.

Pathway

Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.

Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Subunit structure

Homodimer. Ref.5

Sequence similarities

Belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family. MET8 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274Siroheme biosynthesis protein MET8
PRO_0000096446

Regions

Nucleotide binding23 – 242NAD
Nucleotide binding43 – 453NAD

Sites

Active site1411Proton acceptor Potential
Binding site931NAD; via amide nitrogen

Experimental info

Mutagenesis221G → D: Loss of dehydrogenase activity. No effect on chelatase activity. Ref.5
Mutagenesis1411D → A: Loss of chelatase and dehydrogenase activity. Ref.5
Mutagenesis2371H → A: No effect on dehydrogenase or chelatase activity. Ref.5
Sequence conflict151K → R Ref.5
Sequence conflict331I → M Ref.5
Sequence conflict611E → K Ref.5
Sequence conflict1021D → N Ref.5

Secondary structure

............................................. 274
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P15807 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 0EE9A6DE8A43673E

FASTA27431,918
        10         20         30         40         50         60 
MVKSLQLAHQ LKDKKILLIG GGEVGLTRLY KLIPTGCKLT LVSPDLHKSI IPKFGKFIQN 

        70         80         90        100        110        120 
EDQPDYREDA KRFINPNWDP TKNEIYEYIR SDFKDEYLDL EDENDAWYII MTCIPDHPES 

       130        140        150        160        170        180 
ARIYHLCKER FGKQQLVNVA DKPDLCDFYF GANLEIGDRL QILISTNGLS PRFGALVRDE 

       190        200        210        220        230        240 
IRNLFTQMGD LALEDAVVKL GELRRGIRLL APDDKDVKYR MDWARRCTDL FGIQHCHNID 

       250        260        270 
VKRLLDLFKV MFQEQNCSLQ FPPRERLLSE YCSS 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the MET8 gene of Saccharomyces cerevisiae."
Cherest H., Thomas D., Surdin-Kerjan Y.
Nucleic Acids Res. 18:659-659(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26786 / X2180-1A.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The role of Saccharomyces cerevisiae Met1p and Met8p in sirohaem and cobalamin biosynthesis."
Raux E., McVeigh T., Peters S.E., Leustek T., Warren M.J.
Biochem. J. 338:701-708(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase."
Schubert H.L., Raux E., Brindley A.A., Leech H.K., Wilson K.S., Hill C.P., Warren M.J.
EMBO J. 21:2068-2075(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 11-274 IN COMPLEX WITH NAD, SUBUNIT, MUTAGENESIS OF GLY-22; ASP-141 AND HIS-237.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17271 Genomic DNA. Translation: CAA35173.1.
Z36082 Genomic DNA. Translation: CAA85177.1.
BK006936 Genomic DNA. Translation: DAA07329.1.
PIRS20155.
RefSeqNP_009772.1. NM_001178561.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KYQX-ray2.20A/B/C1-274[»]
ProteinModelPortalP15807.
SMRP15807. Positions 1-273.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32910. 16 interactions.
DIPDIP-4944N.
MINTMINT-553462.

Proteomic databases

MaxQBP15807.
PaxDbP15807.
PRIDEP15807.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR213W; YBR213W; YBR213W.
GeneID852514.
KEGGsce:YBR213W.

Organism-specific databases

CYGDYBR213w.
SGDS000000417. MET8.

Phylogenomic databases

eggNOGCOG1648.
HOGENOMHOG000181024.
KOK02304.
OMAVCCCIDD.
OrthoDBEOG7WHHP1.

Enzyme and pathway databases

BioCycMetaCyc:G3O-29150-MONOMER.
YEAST:G3O-29150-MONOMER.
BRENDA4.99.1.4. 984.
UniPathwayUPA00262; UER00222.
UPA00262; UER00376.

Gene expression databases

GenevestigatorP15807.

Family and domain databases

Gene3D1.10.3280.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR028161. Met8.
IPR028162. Met8_C.
IPR016040. NAD(P)-bd_dom.
IPR028281. Sirohaem_synthase_central.
IPR006367. Sirohaem_synthase_N.
[Graphical view]
PANTHERPTHR21091:SF88. PTHR21091:SF88. 1 hit.
PfamPF13241. NAD_binding_7. 1 hit.
PF14823. Sirohm_synth_C. 1 hit.
PF14824. Sirohm_synth_M. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP15807.
NextBio971538.

Entry information

Entry nameMET8_YEAST
AccessionPrimary (citable) accession number: P15807
Secondary accession number(s): D6VQK9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways