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P15807

- MET8_YEAST

UniProt

P15807 - MET8_YEAST

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Protein

Siroheme biosynthesis protein MET8

Gene

MET8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of precorrin-2 into siroheme. This reaction consist of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme.1 Publication

Catalytic activityi

Precorrin-2 + NAD+ = sirohydrochlorin + NADH.
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931NAD; via amide nitrogen1 Publication
Active sitei141 – 1411Proton acceptorSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi23 – 242NAD1 Publication
Nucleotide bindingi43 – 453NAD1 Publication

GO - Molecular functioni

  1. ferrochelatase activity Source: SGD
  2. precorrin-2 dehydrogenase activity Source: SGD
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. siroheme biosynthetic process Source: SGD
  3. sulfate assimilation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:G3O-29150-MONOMER.
YEAST:G3O-29150-MONOMER.
BRENDAi4.99.1.4. 984.
UniPathwayiUPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme biosynthesis protein MET8
Including the following 2 domains:
Precorrin-2 dehydrogenase (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase (EC:4.99.1.4)
Gene namesi
Name:MET8
Ordered Locus Names:YBR213W
ORF Names:YBR1461
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR213w.
SGDiS000000417. MET8.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi22 – 221G → D: Loss of dehydrogenase activity. No effect on chelatase activity. 1 Publication
Mutagenesisi141 – 1411D → A: Loss of chelatase and dehydrogenase activity. 1 Publication
Mutagenesisi237 – 2371H → A: No effect on dehydrogenase or chelatase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Siroheme biosynthesis protein MET8PRO_0000096446Add
BLAST

Proteomic databases

MaxQBiP15807.
PaxDbiP15807.
PRIDEiP15807.

Expressioni

Gene expression databases

GenevestigatoriP15807.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi32910. 17 interactions.
DIPiDIP-4944N.
MINTiMINT-553462.

Structurei

Secondary structure

1
274
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95Combined sources
Beta strandi15 – 228Combined sources
Helixi23 – 3210Combined sources
Helixi33 – 353Combined sources
Beta strandi38 – 469Combined sources
Helixi50 – 545Combined sources
Helixi56 – 583Combined sources
Beta strandi72 – 743Combined sources
Beta strandi86 – 894Combined sources
Helixi95 – 984Combined sources
Beta strandi107 – 1126Combined sources
Helixi117 – 13115Combined sources
Beta strandi135 – 1395Combined sources
Helixi143 – 1453Combined sources
Beta strandi146 – 1494Combined sources
Beta strandi152 – 1565Combined sources
Turni157 – 1593Combined sources
Beta strandi160 – 16910Combined sources
Helixi171 – 18818Combined sources
Helixi193 – 21018Combined sources
Helixi214 – 2163Combined sources
Helixi217 – 23519Combined sources
Helixi236 – 2383Combined sources
Helixi241 – 25212Combined sources
Turni253 – 2564Combined sources
Helixi264 – 2707Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KYQX-ray2.20A/B/C1-274[»]
ProteinModelPortaliP15807.
SMRiP15807. Positions 1-273.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15807.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1648.
HOGENOMiHOG000181024.
InParanoidiP15807.
KOiK02304.
OMAiVCCCIDD.
OrthoDBiEOG7WHHP1.

Family and domain databases

Gene3Di1.10.3280.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR028161. Met8.
IPR028162. Met8_C.
IPR016040. NAD(P)-bd_dom.
IPR028281. Sirohaem_synthase_central.
IPR006367. Sirohaem_synthase_N.
[Graphical view]
PANTHERiPTHR21091:SF88. PTHR21091:SF88. 1 hit.
PfamiPF13241. NAD_binding_7. 1 hit.
PF14823. Sirohm_synth_C. 1 hit.
PF14824. Sirohm_synth_M. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15807-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVKSLQLAHQ LKDKKILLIG GGEVGLTRLY KLIPTGCKLT LVSPDLHKSI
60 70 80 90 100
IPKFGKFIQN EDQPDYREDA KRFINPNWDP TKNEIYEYIR SDFKDEYLDL
110 120 130 140 150
EDENDAWYII MTCIPDHPES ARIYHLCKER FGKQQLVNVA DKPDLCDFYF
160 170 180 190 200
GANLEIGDRL QILISTNGLS PRFGALVRDE IRNLFTQMGD LALEDAVVKL
210 220 230 240 250
GELRRGIRLL APDDKDVKYR MDWARRCTDL FGIQHCHNID VKRLLDLFKV
260 270
MFQEQNCSLQ FPPRERLLSE YCSS
Length:274
Mass (Da):31,918
Last modified:April 1, 1990 - v1
Checksum:i0EE9A6DE8A43673E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151K → R(PubMed:11980703)Curated
Sequence conflicti33 – 331I → M(PubMed:11980703)Curated
Sequence conflicti61 – 611E → K(PubMed:11980703)Curated
Sequence conflicti102 – 1021D → N(PubMed:11980703)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17271 Genomic DNA. Translation: CAA35173.1.
Z36082 Genomic DNA. Translation: CAA85177.1.
BK006936 Genomic DNA. Translation: DAA07329.1.
PIRiS20155.
RefSeqiNP_009772.1. NM_001178561.1.

Genome annotation databases

EnsemblFungiiYBR213W; YBR213W; YBR213W.
GeneIDi852514.
KEGGisce:YBR213W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17271 Genomic DNA. Translation: CAA35173.1 .
Z36082 Genomic DNA. Translation: CAA85177.1 .
BK006936 Genomic DNA. Translation: DAA07329.1 .
PIRi S20155.
RefSeqi NP_009772.1. NM_001178561.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KYQ X-ray 2.20 A/B/C 1-274 [» ]
ProteinModelPortali P15807.
SMRi P15807. Positions 1-273.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32910. 17 interactions.
DIPi DIP-4944N.
MINTi MINT-553462.

Proteomic databases

MaxQBi P15807.
PaxDbi P15807.
PRIDEi P15807.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR213W ; YBR213W ; YBR213W .
GeneIDi 852514.
KEGGi sce:YBR213W.

Organism-specific databases

CYGDi YBR213w.
SGDi S000000417. MET8.

Phylogenomic databases

eggNOGi COG1648.
HOGENOMi HOG000181024.
InParanoidi P15807.
KOi K02304.
OMAi VCCCIDD.
OrthoDBi EOG7WHHP1.

Enzyme and pathway databases

UniPathwayi UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci MetaCyc:G3O-29150-MONOMER.
YEAST:G3O-29150-MONOMER.
BRENDAi 4.99.1.4. 984.

Miscellaneous databases

EvolutionaryTracei P15807.
NextBioi 971538.

Gene expression databases

Genevestigatori P15807.

Family and domain databases

Gene3Di 1.10.3280.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR028161. Met8.
IPR028162. Met8_C.
IPR016040. NAD(P)-bd_dom.
IPR028281. Sirohaem_synthase_central.
IPR006367. Sirohaem_synthase_N.
[Graphical view ]
PANTHERi PTHR21091:SF88. PTHR21091:SF88. 1 hit.
Pfami PF13241. NAD_binding_7. 1 hit.
PF14823. Sirohm_synth_C. 1 hit.
PF14824. Sirohm_synth_M. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the MET8 gene of Saccharomyces cerevisiae."
    Cherest H., Thomas D., Surdin-Kerjan Y.
    Nucleic Acids Res. 18:659-659(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 26786 / X2180-1A.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The role of Saccharomyces cerevisiae Met1p and Met8p in sirohaem and cobalamin biosynthesis."
    Raux E., McVeigh T., Peters S.E., Leustek T., Warren M.J.
    Biochem. J. 338:701-708(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase."
    Schubert H.L., Raux E., Brindley A.A., Leech H.K., Wilson K.S., Hill C.P., Warren M.J.
    EMBO J. 21:2068-2075(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 11-274 IN COMPLEX WITH NAD, SUBUNIT, MUTAGENESIS OF GLY-22; ASP-141 AND HIS-237.

Entry informationi

Entry nameiMET8_YEAST
AccessioniPrimary (citable) accession number: P15807
Secondary accession number(s): D6VQK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 26, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3