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Protein

Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form

Gene

penDE

Organism
Penicillium chrysogenum (Penicillium notatum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Last enzyme in penicillin biosynthetic pathway, which converts isopenicillin N (IPN) to penicillin G, using phenyl-acetyl-CoA or phenoxyacetyl-CoA as acyl donors.

Catalytic activityi

Phenylacetyl-CoA + isopenicillin N + H2O = CoA + penicillin G + L-2-aminohexanedioate.

Pathwayi: penicillin G biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase (PCBAB), N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase (PCBAB)
  2. Isopenicillin N synthase (pcbC)
  3. Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form (penDE)
This subpathway is part of the pathway penicillin G biosynthesis, which is itself part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine, the pathway penicillin G biosynthesis and in Antibiotic biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13369.
BRENDAi2.3.1.164. 4606.
UniPathwayiUPA00149; UER00241.

Protein family/group databases

MEROPSiC45.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form (EC:2.3.1.164)
Alternative name(s):
Isopenicillin-N N-acyltransferase
Cleaved into the following 2 chains:
Gene namesi
Name:penDE
Synonyms:aat
OrganismiPenicillium chrysogenum (Penicillium notatum)
Taxonomic identifieri5076 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaePenicilliumPenicillium chrysogenum complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000205951 – 357Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa formAdd BLAST357
ChainiPRO_00000205961 – 102Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 11 kDa subunitAdd BLAST102
ChainiPRO_0000020597103 – 357Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 29 kDa subunitAdd BLAST255

Keywords - PTMi

Zymogen

Miscellaneous databases

PMAP-CutDBP15802.

Interactioni

Protein-protein interaction databases

STRINGi500485.XP_002569112.1.

Structurei

Secondary structure

1357
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi10 – 20Combined sources11
Helixi22 – 37Combined sources16
Helixi44 – 61Combined sources18
Helixi63 – 76Combined sources14
Helixi80 – 87Combined sources8
Helixi89 – 100Combined sources12
Beta strandi104 – 108Combined sources5
Beta strandi115 – 122Combined sources8
Helixi124 – 129Combined sources6
Beta strandi130 – 136Combined sources7
Beta strandi143 – 148Combined sources6
Beta strandi155 – 158Combined sources4
Beta strandi163 – 167Combined sources5
Helixi181 – 189Combined sources9
Helixi194 – 203Combined sources10
Beta strandi206 – 209Combined sources4
Beta strandi211 – 216Combined sources6
Beta strandi221 – 227Combined sources7
Beta strandi230 – 234Combined sources5
Beta strandi240 – 244Combined sources5
Helixi263 – 277Combined sources15
Helixi283 – 289Combined sources7
Turni294 – 297Combined sources4
Beta strandi298 – 301Combined sources4
Turni306 – 308Combined sources3
Beta strandi312 – 320Combined sources9
Turni321 – 324Combined sources4
Beta strandi325 – 331Combined sources7
Beta strandi337 – 343Combined sources7
Helixi346 – 353Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X1CX-ray1.85A/B/C/D1-357[»]
2X1DX-ray1.64A/B/C/D1-357[»]
2X1EX-ray2.00A/B/C/D1-357[»]
SMRiP15802.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15802.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C45 family.Curated

Phylogenomic databases

eggNOGiENOG410JNG2. Eukaryota.
ENOG410XSKX. LUCA.
PhylomeDBiP15802.

Family and domain databases

InterProiIPR005079. Peptidase_C45.
[Graphical view]
PfamiPF03417. AAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHILCQGTP FEIGYEHGSA AKAVIARSID FAVDLIRGKT KKTDEELKQV
60 70 80 90 100
LSQLGRVIEE RWPKYYEEIR GIAKGAERDV SEIVMLNTRT EFAYGLKAAR
110 120 130 140 150
DGCTTAYCQL PNGALQGQNW DFFSATKENL IRLTIRQAGL PTIKFITEAG
160 170 180 190 200
IIGKVGFNSA GVAVNYNALH LQGLRPTGVP SHIALRIALE STSPSQAYDR
210 220 230 240 250
IVEQGGMAAS AFIMVGNGHE AFGLEFSPTS IRKQVLDANG RMVHTNHCLL
260 270 280 290 300
QHGKNEKELD PLPDSWNRHQ RMEFLLDGFD GTKQAFAQLW ADEDNYPFSI
310 320 330 340 350
CRAYEEGKSR GATLFNIIYD HARREATVRL GRPTNPDEMF VMRFDEEDER

SALNARL
Length:357
Mass (Da):39,939
Last modified:April 1, 1990 - v1
Checksum:i7A05822312D1CF08
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103C → W AA sequence (PubMed:2110531).Curated1
Sequence conflicti125A → P AA sequence (PubMed:2110531).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31454 Genomic DNA. Translation: AAA33692.1.
A15528 Unassigned DNA. Translation: CAA01234.1.
A15359 Unassigned DNA. Translation: CAA01221.1.
PIRiJQ0118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31454 Genomic DNA. Translation: AAA33692.1.
A15528 Unassigned DNA. Translation: CAA01234.1.
A15359 Unassigned DNA. Translation: CAA01221.1.
PIRiJQ0118.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X1CX-ray1.85A/B/C/D1-357[»]
2X1DX-ray1.64A/B/C/D1-357[»]
2X1EX-ray2.00A/B/C/D1-357[»]
SMRiP15802.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi500485.XP_002569112.1.

Protein family/group databases

MEROPSiC45.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410JNG2. Eukaryota.
ENOG410XSKX. LUCA.
PhylomeDBiP15802.

Enzyme and pathway databases

UniPathwayiUPA00149; UER00241.
BioCyciMetaCyc:MONOMER-13369.
BRENDAi2.3.1.164. 4606.

Miscellaneous databases

EvolutionaryTraceiP15802.
PMAP-CutDBP15802.

Family and domain databases

InterProiIPR005079. Peptidase_C45.
[Graphical view]
PfamiPF03417. AAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAAAA_PENCH
AccessioniPrimary (citable) accession number: P15802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The pre-AAT protein is probably synthesized as 40 kDa precursor which is then processed into an 11 kDa (protein A) and a 29 kDa (protein B). The B protein carries AAT activity.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.