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Protein

Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form

Gene

penDE

Organism
Penicillium chrysogenum (Penicillium notatum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Last enzyme in penicillin biosynthetic pathway, which converts isopenicillin N (IPN) to penicillin G, using phenyl-acetyl-CoA or phenoxyacetyl-CoA as acyl donors.

Catalytic activityi

Phenylacetyl-CoA + isopenicillin N + H2O = CoA + penicillin G + L-2-aminohexanedioate.

Pathwayi: penicillin G biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase (PCBAB), N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase (PCBAB)
  2. Isopenicillin N synthase (pcbC)
  3. Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form (penDE)
This subpathway is part of the pathway penicillin G biosynthesis, which is itself part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine, the pathway penicillin G biosynthesis and in Antibiotic biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13369.
BRENDAi2.3.1.164. 4606.
UniPathwayiUPA00149; UER00241.

Protein family/group databases

MEROPSiC45.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form (EC:2.3.1.164)
Alternative name(s):
Isopenicillin-N N-acyltransferase
Cleaved into the following 2 chains:
Gene namesi
Name:penDE
Synonyms:aat
OrganismiPenicillium chrysogenum (Penicillium notatum)
Taxonomic identifieri5076 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaePenicilliumPenicillium chrysogenum complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa formPRO_0000020595Add
BLAST
Chaini1 – 102102Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 11 kDa subunitPRO_0000020596Add
BLAST
Chaini103 – 357255Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 29 kDa subunitPRO_0000020597Add
BLAST

Keywords - PTMi

Zymogen

Miscellaneous databases

PMAP-CutDBP15802.

Interactioni

Protein-protein interaction databases

STRINGi500485.XP_002569112.1.

Structurei

Secondary structure

1
357
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97Combined sources
Helixi10 – 2011Combined sources
Helixi22 – 3716Combined sources
Helixi44 – 6118Combined sources
Helixi63 – 7614Combined sources
Helixi80 – 878Combined sources
Helixi89 – 10012Combined sources
Beta strandi104 – 1085Combined sources
Beta strandi115 – 1228Combined sources
Helixi124 – 1296Combined sources
Beta strandi130 – 1367Combined sources
Beta strandi143 – 1486Combined sources
Beta strandi155 – 1584Combined sources
Beta strandi163 – 1675Combined sources
Helixi181 – 1899Combined sources
Helixi194 – 20310Combined sources
Beta strandi206 – 2094Combined sources
Beta strandi211 – 2166Combined sources
Beta strandi221 – 2277Combined sources
Beta strandi230 – 2345Combined sources
Beta strandi240 – 2445Combined sources
Helixi263 – 27715Combined sources
Helixi283 – 2897Combined sources
Turni294 – 2974Combined sources
Beta strandi298 – 3014Combined sources
Turni306 – 3083Combined sources
Beta strandi312 – 3209Combined sources
Turni321 – 3244Combined sources
Beta strandi325 – 3317Combined sources
Beta strandi337 – 3437Combined sources
Helixi346 – 3538Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2X1CX-ray1.85A/B/C/D1-357[»]
2X1DX-ray1.64A/B/C/D1-357[»]
2X1EX-ray2.00A/B/C/D1-357[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15802.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C45 family.Curated

Phylogenomic databases

eggNOGiENOG410JNG2. Eukaryota.
ENOG410XSKX. LUCA.
PhylomeDBiP15802.

Family and domain databases

InterProiIPR005079. Peptidase_C45.
[Graphical view]
PfamiPF03417. AAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHILCQGTP FEIGYEHGSA AKAVIARSID FAVDLIRGKT KKTDEELKQV
60 70 80 90 100
LSQLGRVIEE RWPKYYEEIR GIAKGAERDV SEIVMLNTRT EFAYGLKAAR
110 120 130 140 150
DGCTTAYCQL PNGALQGQNW DFFSATKENL IRLTIRQAGL PTIKFITEAG
160 170 180 190 200
IIGKVGFNSA GVAVNYNALH LQGLRPTGVP SHIALRIALE STSPSQAYDR
210 220 230 240 250
IVEQGGMAAS AFIMVGNGHE AFGLEFSPTS IRKQVLDANG RMVHTNHCLL
260 270 280 290 300
QHGKNEKELD PLPDSWNRHQ RMEFLLDGFD GTKQAFAQLW ADEDNYPFSI
310 320 330 340 350
CRAYEEGKSR GATLFNIIYD HARREATVRL GRPTNPDEMF VMRFDEEDER

SALNARL
Length:357
Mass (Da):39,939
Last modified:April 1, 1990 - v1
Checksum:i7A05822312D1CF08
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031C → W AA sequence (PubMed:2110531).Curated
Sequence conflicti125 – 1251A → P AA sequence (PubMed:2110531).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31454 Genomic DNA. Translation: AAA33692.1.
A15528 Unassigned DNA. Translation: CAA01234.1.
A15359 Unassigned DNA. Translation: CAA01221.1.
PIRiJQ0118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31454 Genomic DNA. Translation: AAA33692.1.
A15528 Unassigned DNA. Translation: CAA01234.1.
A15359 Unassigned DNA. Translation: CAA01221.1.
PIRiJQ0118.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2X1CX-ray1.85A/B/C/D1-357[»]
2X1DX-ray1.64A/B/C/D1-357[»]
2X1EX-ray2.00A/B/C/D1-357[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi500485.XP_002569112.1.

Protein family/group databases

MEROPSiC45.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410JNG2. Eukaryota.
ENOG410XSKX. LUCA.
PhylomeDBiP15802.

Enzyme and pathway databases

UniPathwayiUPA00149; UER00241.
BioCyciMetaCyc:MONOMER-13369.
BRENDAi2.3.1.164. 4606.

Miscellaneous databases

EvolutionaryTraceiP15802.
PMAP-CutDBP15802.

Family and domain databases

InterProiIPR005079. Peptidase_C45.
[Graphical view]
PfamiPF03417. AAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAAAA_PENCH
AccessioniPrimary (citable) accession number: P15802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The pre-AAT protein is probably synthesized as 40 kDa precursor which is then processed into an 11 kDa (protein A) and a 29 kDa (protein B). The B protein carries AAT activity.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.