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Protein

DNA polymerase gamma

Gene

MIP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the replication of mitochondrial DNA.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

GO - Molecular functioni

  • 3'-5' exonuclease activity Source: SGD
  • DNA binding Source: UniProtKB-KW
  • DNA-directed DNA polymerase activity Source: SGD

GO - Biological processi

  • mitochondrial DNA replication Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding, Magnesium

Enzyme and pathway databases

BioCyciYEAST:G3O-33807-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase gamma (EC:2.7.7.7)
Alternative name(s):
Mitochondrial DNA polymerase catalytic subunit
Gene namesi
Name:MIP1
Ordered Locus Names:YOR330C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR330C.
SGDiS000005857. MIP1.

Subcellular locationi

GO - Cellular componenti

  • gamma DNA polymerase complex Source: InterPro
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12541254DNA polymerase gammaPRO_0000101278Add
BLAST

Proteomic databases

MaxQBiP15801.

PTM databases

iPTMnetiP15801.

Interactioni

Protein-protein interaction databases

BioGridi34715. 155 interactions.
IntActiP15801. 3 interactions.
MINTiMINT-4503978.

Structurei

3D structure databases

ProteinModelPortaliP15801.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000000453.
HOGENOMiHOG000176668.
InParanoidiP15801.
KOiK02332.
OMAiDWSGQEI.
OrthoDBiEOG7WDN9T.

Family and domain databases

InterProiIPR019760. DNA-dir_DNA_pol_A_CS.
IPR002297. DNA-dir_DNA_pol_A_mt.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10267. PTHR10267. 1 hit.
PfamiPF00476. DNA_pol_A. 1 hit.
[Graphical view]
PIRSFiPIRSF000797. DNA_pol_mt. 1 hit.
PRINTSiPR00867. DNAPOLG.
SMARTiSM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKLMVRSEC MLRMVRRRPL RVQFCARWFS TKKNTAEAPR INPVGIQYLG
60 70 80 90 100
ESLQRQVFGS CGGKDEVEQS DKLMELSKKS LKDHGLWGKK TLITDPISFP
110 120 130 140 150
LPPLQGRSLD EHFQKIGRFN SEPYKSFCED KFTEMVARPA EWLRKPGWVK
160 170 180 190 200
YVPGMAPVEV AYPDEELVVF DVETLYNVSD YPTLATALSS TAWYLWCSPF
210 220 230 240 250
ICGGDDPAAL IPLNTLNKEQ VIIGHNVAYD RARVLEEYNF RDSKAFFLDT
260 270 280 290 300
QSLHIASFGL CSRQRPMFMK NNKKKEAEVE SEVHPEISIE DYDDPWLNVS
310 320 330 340 350
ALNSLKDVAK FHCKIDLDKT DRDFFASTDK STIIENFQKL VNYCATDVTA
360 370 380 390 400
TSQVFDEIFP VFLKKCPHPV SFAGLKSLSK CILPTKLNDW NDYLNSSESL
410 420 430 440 450
YQQSKVQIES KIVQIIKDIV LLKDKPDFYL KDPWLSQLDW TTKPLRLTKK
460 470 480 490 500
GVPAKCQKLP GFPEWYRQLF PSKDTVEPKI TIKSRIIPIL FKLSWENSPV
510 520 530 540 550
IWSKESGWCF NVPHEQVETY KAKNYVLADS VSQEEEEIRT HNLGLQCTGV
560 570 580 590 600
LFKVPHPNGP TFNCTNLLTK SYNHFFEKGV LKSESELAHQ ALQINSSGSY
610 620 630 640 650
WMSARERIQS QFVVPSCKFP NEFQSLSAKS SLNNEKTNDL AIIIPKIVPM
660 670 680 690 700
GTITRRAVEN AWLTASNAKA NRIGSELKTQ VKAPPGYCFV GADVDSEELW
710 720 730 740 750
IASLVGDSIF NVHGGTAIGW MCLEGTKNEG TDLHTKTAQI LGCSRNEAKI
760 770 780 790 800
FNYGRIYGAG AKFASQLLKR FNPSLTDEET KKIANKLYEN TKGKTKRSKL
810 820 830 840 850
FKKFWYGGSE SILFNKLESI AEQETPKTPV LGCGITYSLM KKNLRANSFL
860 870 880 890 900
PSRINWAIQS SGVDYLHLLC CSMEYIIKKY NLEARLCISI HDEIRFLVSE
910 920 930 940 950
KDKYRAAMAL QISNIWTRAM FCQQMGINEL PQNCAFFSQV DIDSVIRKEV
960 970 980 990 1000
NMDCITPSNK TAIPHGEALD INQLLDKSNS KLGKPNLDID SKVSQYAYNY
1010 1020 1030 1040 1050
REPVFEEYNK SYTPEFLKYF LAMQVQSDKR DVNRLEDEYL RECTSKEYAR
1060 1070 1080 1090 1100
DGNTAEYSLL DYIKDVEKGK RTKVRIMGSN FLDGTKNAKA DQRIRLPVNM
1110 1120 1130 1140 1150
PDYPTLHKIA NDSAIPEKQL LENRRKKENR IDDENKKKLT RKKNTTPMER
1160 1170 1180 1190 1200
KYKRVYGGRK AFEAFYECAN KPLDYTLETE KQFFNIPIDG VIDDVLNDKS
1210 1220 1230 1240 1250
NYKKKPSQAR TASSSPIRKT AKAVHSKKLP ARKSSTTNRN LVELERDITI

SREY
Length:1,254
Mass (Da):143,502
Last modified:October 1, 1996 - v3
Checksum:i80EE686E8EA9F2EC
GO

Sequence cautioni

The sequence CAA99652.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81S → F in AAA17543 (PubMed:2684980).Curated
Sequence conflicti35 – 351T → A in AAA17543 (PubMed:2684980).Curated
Sequence conflicti222 – 2221I → V in AAA17543 (PubMed:2684980).Curated
Sequence conflicti357 – 3571E → K in AAA17543 (PubMed:2684980).Curated
Sequence conflicti540 – 5412TH → MN in AAA17543 (PubMed:2684980).Curated
Sequence conflicti616 – 6161S → N in AAA17543 (PubMed:2684980).Curated
Sequence conflicti661 – 6611A → T in AAA17543 (PubMed:2684980).Curated
Sequence conflicti978 – 9781S → P in AAA17543 (PubMed:2684980).Curated
Sequence conflicti986 – 9861N → S in AAA17543 (PubMed:2684980).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05117 Unassigned DNA. Translation: AAA17543.1.
Z49821 Genomic DNA. Translation: CAA89977.1.
Z75238 Genomic DNA. Translation: CAA99652.1. Different initiation.
U42227 Genomic DNA. Translation: AAA85442.1.
BK006948 Genomic DNA. Translation: DAA11093.1.
PIRiS62062. A32686.
RefSeqiNP_014975.2. NM_001183750.1.

Genome annotation databases

EnsemblFungiiYOR330C; YOR330C; YOR330C.
GeneIDi854508.
KEGGisce:YOR330C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05117 Unassigned DNA. Translation: AAA17543.1.
Z49821 Genomic DNA. Translation: CAA89977.1.
Z75238 Genomic DNA. Translation: CAA99652.1. Different initiation.
U42227 Genomic DNA. Translation: AAA85442.1.
BK006948 Genomic DNA. Translation: DAA11093.1.
PIRiS62062. A32686.
RefSeqiNP_014975.2. NM_001183750.1.

3D structure databases

ProteinModelPortaliP15801.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34715. 155 interactions.
IntActiP15801. 3 interactions.
MINTiMINT-4503978.

PTM databases

iPTMnetiP15801.

Proteomic databases

MaxQBiP15801.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR330C; YOR330C; YOR330C.
GeneIDi854508.
KEGGisce:YOR330C.

Organism-specific databases

EuPathDBiFungiDB:YOR330C.
SGDiS000005857. MIP1.

Phylogenomic databases

GeneTreeiENSGT00390000000453.
HOGENOMiHOG000176668.
InParanoidiP15801.
KOiK02332.
OMAiDWSGQEI.
OrthoDBiEOG7WDN9T.

Enzyme and pathway databases

BioCyciYEAST:G3O-33807-MONOMER.

Miscellaneous databases

PROiP15801.

Family and domain databases

InterProiIPR019760. DNA-dir_DNA_pol_A_CS.
IPR002297. DNA-dir_DNA_pol_A_mt.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10267. PTHR10267. 1 hit.
PfamiPF00476. DNA_pol_A. 1 hit.
[Graphical view]
PIRSFiPIRSF000797. DNA_pol_mt. 1 hit.
PRINTSiPR00867. DNAPOLG.
SMARTiSM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the nuclear gene MIP1 encoding the catalytic subunit of the yeast mitochondrial DNA polymerase."
    Foury F.
    J. Biol. Chem. 264:20552-20560(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. Foury F.
    Submitted (JUN-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I."
    Parle-McDermott A.G., Hand N.J., Goulding S.E., Wolfe K.H.
    Yeast 12:999-1004(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Song J.M., Cheung E., Rabinowitz J.C.
    Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE OF 987-1254.
    Strain: S288c / GRF88.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDPOG_YEAST
AccessioniPrimary (citable) accession number: P15801
Secondary accession number(s): D6W327, Q08785
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.