Reviewed, UniProtKB/Swiss-Prot P15797 (E13B_TOBAC)

Names and origin

Protein names	Recommended name:     Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform     EC=3.2.1.39 Alternative name(s):     (1->3)-beta-glucan endohydrolase       Short name=(1->3)-beta-glucanase     Beta-1,3-endoglucanase, basic
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Vacuole.
Tissue specificity	Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.
Induction	By ethylene.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Vacuole
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33
	Chain	34 – 349	316	Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform		PRO_0000011869
	Propeptide	350 – 371	22	Removed in mature form		PRO_0000011870
Sites
	Active site	274	1	Nucleophile By similarity
	Active site	330	1	Proton donor By similarity
Amino acid modifications
	Modified residue	34	1	Pyrrolidone carboxylic acid Probable
	Glycosylation	362	1	N-linked (GlcNAc...)
Natural variations
	Natural variant	193	1	T → V
	Natural variant	296	1	N → Y
	Natural variant	299	1	Q → P
	Natural variant	346	1	I → L

Sequences

	Sequence		Length	Mass (Da)
	P15797-1 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 8BB1C273104974C8	FASTA	371	40,510
	10 20 30 40 50 60 MSTSSHKHNT PQMAAITLLG LLLVASSIDI AGAQSIGVCY GMLGNNLPNH WEVIQLYKSR 70 80 90 100 110 120 NIGRLRLYDP NHGALQALKG SNIEVMLGLP NSDVKHIASG MEHARWWVQK NVKDFWPDVK 130 140 150 160 170 180 IKYIAVGNEI SPVTGTSYLT SFLTPAMVNI YKAIGEAGLG NNIKVSTSVD MTLIGNSYPP 190 200 210 220 230 240 SQGSFRNDAR WFTDPIVGFL RDTRAPLLVN IYPYFSYSGN PGQISLPYSL FTAPNVVVQD 250 260 270 280 290 300 GSRQYRNLFD AMLDSVYAAL ERSGGASVGI VVSESGWPSA GAFGATYDNA ATYLRNLIQH 310 320 330 340 350 360 AKEGSPRKPG PIETYIFAMF DENNKNPELE KHFGLFSPNK QPKYNINFGV SGGVWDSSVE 370 TNATASLVSE M

References

[1]	"Evidence for N- and C-terminal processing of a plant defence-related enzyme: the primary structure of tobacco pre-pro-beta-1,3-glucanase." Shinshi H., Wenzler H., Neuhaus J.-M., Felix G., Hofsteenge J., Meins F. Jr. Proc. Natl. Acad. Sci. U.S.A. 85:5541-5545(1988) [PubMed: 16593965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system." van den Bulcke M., Bauw G., Castresana C., van Montagu M., Vandekerckhove J. Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989) [PubMed: 16594025] [Abstract] Cited for: PROTEIN SEQUENCE OF 67-91; 106-110; 194-217; 263-291 AND 344-349.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M20618 mRNA. Translation: AAA34080.1. M20619 mRNA. Translation: AAA34081.1. M20620 mRNA. Translation: AAA34082.1.
PIR	A30758.
3D structure databases
HSSP	HSSP built from PDB template 1GHS based on UniProtKB P15737.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Enzyme and pathway databases
BRENDA	3.2.1.39. 298.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13B_TOBAC

Accession

Primary (citable) accession number: P15797

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)