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P15797

- E13B_TOBAC

UniProt

P15797 - E13B_TOBAC

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Protein

Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Implicated in the defense of plants against pathogens.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei274 – 2741NucleophileBy similarity
Active sitei330 – 3301Proton donorBy similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase, basic
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. vacuole Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Add
BLAST
Chaini34 – 349316Glucan endo-1,3-beta-glucosidase, basic vacuolar isoformPRO_0000011869Add
BLAST
Propeptidei350 – 37122Removed in mature formPRO_0000011870Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341Pyrrolidone carboxylic acidCurated
Glycosylationi362 – 3621N-linked (GlcNAc...)

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

Inductioni

By ethylene.

Structurei

3D structure databases

ProteinModelPortaliP15797.
SMRiP15797. Positions 36-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15797-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTSSHKHNT PQMAAITLLG LLLVASSIDI AGAQSIGVCY GMLGNNLPNH
60 70 80 90 100
WEVIQLYKSR NIGRLRLYDP NHGALQALKG SNIEVMLGLP NSDVKHIASG
110 120 130 140 150
MEHARWWVQK NVKDFWPDVK IKYIAVGNEI SPVTGTSYLT SFLTPAMVNI
160 170 180 190 200
YKAIGEAGLG NNIKVSTSVD MTLIGNSYPP SQGSFRNDAR WFTDPIVGFL
210 220 230 240 250
RDTRAPLLVN IYPYFSYSGN PGQISLPYSL FTAPNVVVQD GSRQYRNLFD
260 270 280 290 300
AMLDSVYAAL ERSGGASVGI VVSESGWPSA GAFGATYDNA ATYLRNLIQH
310 320 330 340 350
AKEGSPRKPG PIETYIFAMF DENNKNPELE KHFGLFSPNK QPKYNINFGV
360 370
SGGVWDSSVE TNATASLVSE M
Length:371
Mass (Da):40,510
Last modified:June 1, 1994 - v2
Checksum:i8BB1C273104974C8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti193 – 1931T → V.
Natural varianti296 – 2961N → Y.
Natural varianti299 – 2991Q → P.
Natural varianti346 – 3461I → L.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20618 mRNA. Translation: AAA34080.1.
M20619 mRNA. Translation: AAA34081.1.
M20620 mRNA. Translation: AAA34082.1.
PIRiA30758.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20618 mRNA. Translation: AAA34080.1 .
M20619 mRNA. Translation: AAA34081.1 .
M20620 mRNA. Translation: AAA34082.1 .
PIRi A30758.

3D structure databases

ProteinModelPortali P15797.
SMRi P15797. Positions 36-348.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evidence for N- and C-terminal processing of a plant defence-related enzyme: the primary structure of tobacco pre-pro-beta-1,3-glucanase."
    Shinshi H., Wenzler H., Neuhaus J.-M., Felix G., Hofsteenge J., Meins F. Jr.
    Proc. Natl. Acad. Sci. U.S.A. 85:5541-5545(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system."
    van den Bulcke M., Bauw G., Castresana C., van Montagu M., Vandekerckhove J.
    Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 67-91; 106-110; 194-217; 263-291 AND 344-349.

Entry informationi

Entry nameiE13B_TOBAC
AccessioniPrimary (citable) accession number: P15797
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3