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P15797 (E13B_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform

EC=3.2.1.39
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase, basic
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length371 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in the defense of plants against pathogens.

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Vacuole.

Tissue specificity

Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

Induction

By ethylene.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentVacuole
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Pyrrolidone carboxylic acid
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

defense response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentvacuole

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglucan endo-1,3-beta-D-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333
Chain34 – 349316Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform
PRO_0000011869
Propeptide350 – 37122Removed in mature form
PRO_0000011870

Sites

Active site2741Nucleophile By similarity
Active site3301Proton donor By similarity

Amino acid modifications

Modified residue341Pyrrolidone carboxylic acid Probable
Glycosylation3621N-linked (GlcNAc...)

Natural variations

Natural variant1931T → V.
Natural variant2961N → Y.
Natural variant2991Q → P.
Natural variant3461I → L.

Sequences

Sequence LengthMass (Da)Tools
P15797 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: 8BB1C273104974C8

FASTA37140,510
        10         20         30         40         50         60 
MSTSSHKHNT PQMAAITLLG LLLVASSIDI AGAQSIGVCY GMLGNNLPNH WEVIQLYKSR 

        70         80         90        100        110        120 
NIGRLRLYDP NHGALQALKG SNIEVMLGLP NSDVKHIASG MEHARWWVQK NVKDFWPDVK 

       130        140        150        160        170        180 
IKYIAVGNEI SPVTGTSYLT SFLTPAMVNI YKAIGEAGLG NNIKVSTSVD MTLIGNSYPP 

       190        200        210        220        230        240 
SQGSFRNDAR WFTDPIVGFL RDTRAPLLVN IYPYFSYSGN PGQISLPYSL FTAPNVVVQD 

       250        260        270        280        290        300 
GSRQYRNLFD AMLDSVYAAL ERSGGASVGI VVSESGWPSA GAFGATYDNA ATYLRNLIQH 

       310        320        330        340        350        360 
AKEGSPRKPG PIETYIFAMF DENNKNPELE KHFGLFSPNK QPKYNINFGV SGGVWDSSVE 

       370 
TNATASLVSE M 

« Hide

References

[1]"Evidence for N- and C-terminal processing of a plant defence-related enzyme: the primary structure of tobacco pre-pro-beta-1,3-glucanase."
Shinshi H., Wenzler H., Neuhaus J.-M., Felix G., Hofsteenge J., Meins F. Jr.
Proc. Natl. Acad. Sci. U.S.A. 85:5541-5545(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system."
van den Bulcke M., Bauw G., Castresana C., van Montagu M., Vandekerckhove J.
Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 67-91; 106-110; 194-217; 263-291 AND 344-349.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20618 mRNA. Translation: AAA34080.1.
M20619 mRNA. Translation: AAA34081.1.
M20620 mRNA. Translation: AAA34082.1.
PIRA30758.

3D structure databases

ProteinModelPortalP15797.
SMRP15797. Positions 36-348.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE13B_TOBAC
AccessionPrimary (citable) accession number: P15797
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries