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P15797

- E13B_TOBAC

UniProt

P15797 - E13B_TOBAC

Protein

Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Implicated in the defense of plants against pathogens.

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei274 – 2741NucleophileBy similarity
    Active sitei330 – 3301Proton donorBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Plant defense

    Protein family/group databases

    CAZyiGH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase
    Short name:
    (1->3)-beta-glucanase
    Beta-1,3-endoglucanase, basic
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    Subcellular locationi

    GO - Cellular componenti

    1. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Add
    BLAST
    Chaini34 – 349316Glucan endo-1,3-beta-glucosidase, basic vacuolar isoformPRO_0000011869Add
    BLAST
    Propeptidei350 – 37122Removed in mature formPRO_0000011870Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei34 – 341Pyrrolidone carboxylic acidCurated
    Glycosylationi362 – 3621N-linked (GlcNAc...)

    Keywords - PTMi

    Glycoprotein, Pyrrolidone carboxylic acid

    Expressioni

    Tissue specificityi

    Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

    Inductioni

    By ethylene.

    Structurei

    3D structure databases

    ProteinModelPortaliP15797.
    SMRiP15797. Positions 36-348.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15797-1 [UniParc]FASTAAdd to Basket

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    MSTSSHKHNT PQMAAITLLG LLLVASSIDI AGAQSIGVCY GMLGNNLPNH    50
    WEVIQLYKSR NIGRLRLYDP NHGALQALKG SNIEVMLGLP NSDVKHIASG 100
    MEHARWWVQK NVKDFWPDVK IKYIAVGNEI SPVTGTSYLT SFLTPAMVNI 150
    YKAIGEAGLG NNIKVSTSVD MTLIGNSYPP SQGSFRNDAR WFTDPIVGFL 200
    RDTRAPLLVN IYPYFSYSGN PGQISLPYSL FTAPNVVVQD GSRQYRNLFD 250
    AMLDSVYAAL ERSGGASVGI VVSESGWPSA GAFGATYDNA ATYLRNLIQH 300
    AKEGSPRKPG PIETYIFAMF DENNKNPELE KHFGLFSPNK QPKYNINFGV 350
    SGGVWDSSVE TNATASLVSE M 371
    Length:371
    Mass (Da):40,510
    Last modified:June 1, 1994 - v2
    Checksum:i8BB1C273104974C8
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti193 – 1931T → V.
    Natural varianti296 – 2961N → Y.
    Natural varianti299 – 2991Q → P.
    Natural varianti346 – 3461I → L.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20618 mRNA. Translation: AAA34080.1.
    M20619 mRNA. Translation: AAA34081.1.
    M20620 mRNA. Translation: AAA34082.1.
    PIRiA30758.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20618 mRNA. Translation: AAA34080.1 .
    M20619 mRNA. Translation: AAA34081.1 .
    M20620 mRNA. Translation: AAA34082.1 .
    PIRi A30758.

    3D structure databases

    ProteinModelPortali P15797.
    SMRi P15797. Positions 36-348.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH17. Glycoside Hydrolase Family 17.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evidence for N- and C-terminal processing of a plant defence-related enzyme: the primary structure of tobacco pre-pro-beta-1,3-glucanase."
      Shinshi H., Wenzler H., Neuhaus J.-M., Felix G., Hofsteenge J., Meins F. Jr.
      Proc. Natl. Acad. Sci. U.S.A. 85:5541-5545(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system."
      van den Bulcke M., Bauw G., Castresana C., van Montagu M., Vandekerckhove J.
      Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 67-91; 106-110; 194-217; 263-291 AND 344-349.

    Entry informationi

    Entry nameiE13B_TOBAC
    AccessioniPrimary (citable) accession number: P15797
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3