Calcium/calmodulin-dependent protein kinase type II delta chain

Names and origin

Protein names

Recommended name:
    Calcium/calmodulin-dependent protein kinase type II delta chain
      Short name=CaM-kinase II delta chain
      Short name=CaM kinase II subunit delta
      Short name=CaMK-II subunit delta
    EC=2.7.11.17

Gene names

Name:

Camk2d

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Autophosphorylation of CAMK2 plays an important role in the regulation of the kinase activity.
Subunit structure	CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
Tissue specificity	Isoform Delta 1 is the predominant form in the brain, isoform Delta 2 and isoform Delta 3 predominate in the aorta and isoform Delta 4 in skeletal muscle.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.

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Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cellular potassium ion homeostasis Inferred from mutant phenotype. Source: RGD positive regulation of cell proliferation Inferred from mutant phenotype. Source: RGD protein amino acid autophosphorylation Inferred from direct assay. Source: RGD regulation of G2/M transition of mitotic cell cycle Inferred from mutant phenotype. Source: RGD response to hypoxia Inferred from mutant phenotype. Source: RGD
Cellular component	calcium- and calmodulin-dependent protein kinase complex Traceable author statement. Source: UniProtKB cytosol Ref.3 Inferred from physical interaction. Source: RGD nucleus Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

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Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Isoform Delta 1 (identifier: P15791-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Delta 2 (identifier: P15791-2) The sequence of this isoform differs from the canonical sequence as follows: 329-362: Missing.

Isoform Delta 3 (identifier: P15791-3) The sequence of this isoform differs from the canonical sequence as follows: 329-335: INNKANV → KRKSSSV 337-359: Missing. 360-362: GNK → QMM

Isoform Delta 4 (identifier: P15791-4) The sequence of this isoform differs from the canonical sequence as follows: 349-362: Missing.

Isoform Delta 5 (identifier: P15791-5) The sequence of this isoform differs from the canonical sequence as follows: 329-362: Missing. 512-533: KPPCIPNGKENFSGGTSLWQNI → N

Isoform Delta 6 (identifier: P15791-6) The sequence of this isoform differs from the canonical sequence as follows: 512-533: KPPCIPNGKENFSGGTSLWQNI → N

Isoform Delta 7 (identifier: P15791-7) The sequence of this isoform differs from the canonical sequence as follows: 349-362: Missing. 512-533: KPPCIPNGKENFSGGTSLWQNI → N

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 533

533

Calcium/calmodulin-dependent protein kinase type II delta chain

PRO_0000086100

Regions

Domain

14 – 272

259

Protein kinase

Nucleotide binding

20 – 28

9

ATP By similarity

Region

291 – 301

11

Calmodulin-binding By similarity

Sites

Active site

136

1

Proton acceptor By similarity

Binding site

43

1

ATP By similarity

Amino acid modifications

Modified residue

3

1

Phosphoserine By similarity

Modified residue

231

1

Phosphotyrosine By similarity

Modified residue

287

1

Phosphothreonine By similarity

Modified residue

315

1

Phosphoserine By similarity

Modified residue

319

1

Phosphoserine By similarity

Modified residue

364

1

Phosphoserine By similarity

Modified residue

365

1

Phosphothreonine By similarity

Modified residue

367

1

Phosphoserine By similarity

Modified residue

371

1

Phosphothreonine By similarity

Modified residue

506

1

Phosphoserine By similarity

Natural variations

Alternative sequence

329 – 362

34

Missing in isoform Delta 2 and isoform Delta 5.

VSP_004784

Alternative sequence

329 – 335

7

INNKANV → KRKSSSV in isoform Delta 3.

VSP_004785

Alternative sequence

337 – 359

23

Missing in isoform Delta 3.

VSP_004786

Alternative sequence

349 – 362

14

Missing in isoform Delta 4 and isoform Delta 7.

VSP_004788

Alternative sequence

360 – 362

3

GNK → QMM in isoform Delta 3.

VSP_004787

Alternative sequence

512 – 533

22

KPPCI…LWQNI → N in isoform Delta 5, isoform Delta 6 and isoform Delta 7.

VSP_012043

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Sequences

Sequence		Length	Mass (Da)
Isoform Delta 1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: E41BCB2B5A00E7CA Show »	FASTA	533	60,081
10 20 30 40 50 60 MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSAAKSL LKKPDGVKIN NKANVVTSPK ENIPTPALEP QTTVIHNPDG 370 380 390 400 410 420 NKESTESSNT TIEDEDVKAR KQEIIKVTEQ LIEAINNGDF EAYTKICDPG LTAFEPEALG 430 440 450 460 470 480 NLVEGMDFHR FYFENALPKI NKPIHTIILN PHVHLVGDDA ACIAYIRLTQ YMDGNGMPKT 490 500 510 520 530 MQSEETRVWH RRDGKWQNIH FHRSGSPTVP IKPPCIPNGK ENFSGGTSLW QNI « Hide
Isoform Delta 2. Checksum: A200989BB0E857D6 Show »	FASTA	499	56,447
10 20 30 40 50 60 MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSAAKSL LKKPDGVKES TESSNTTIED EDVKARKQEI IKVTEQLIEA 370 380 390 400 410 420 INNGDFEAYT KICDPGLTAF EPEALGNLVE GMDFHRFYFE NALPKINKPI HTIILNPHVH 430 440 450 460 470 480 LVGDDAACIA YIRLTQYMDG NGMPKTMQSE ETRVWHRRDG KWQNIHFHRS GSPTVPIKPP 490 CIPNGKENFS GGTSLWQNI « Hide
Isoform Delta 3. Checksum: D21D28E2314C20AB Show »	FASTA	510	57,709
10 20 30 40 50 60 MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSAAKSL LKKPDGVKKR KSSSVVQMME STESSNTTIE DEDVKARKQE 370 380 390 400 410 420 IIKVTEQLIE AINNGDFEAY TKICDPGLTA FEPEALGNLV EGMDFHRFYF ENALPKINKP 430 440 450 460 470 480 IHTIILNPHV HLVGDDAACI AYIRLTQYMD GNGMPKTMQS EETRVWHRRD GKWQNIHFHR 490 500 510 SGSPTVPIKP PCIPNGKENF SGGTSLWQNI « Hide
Isoform Delta 4. Checksum: D8D44571AB08AE3B Show »	FASTA	519	58,549
10 20 30 40 50 60 MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSAAKSL LKKPDGVKIN NKANVVTSPK ENIPTPALES TESSNTTIED 370 380 390 400 410 420 EDVKARKQEI IKVTEQLIEA INNGDFEAYT KICDPGLTAF EPEALGNLVE GMDFHRFYFE 430 440 450 460 470 480 NALPKINKPI HTIILNPHVH LVGDDAACIA YIRLTQYMDG NGMPKTMQSE ETRVWHRRDG 490 500 510 KWQNIHFHRS GSPTVPIKPP CIPNGKENFS GGTSLWQNI « Hide
Isoform Delta 5. Checksum: D58353ACB0B7CEBF Show »	FASTA	478	54,191
10 20 30 40 50 60 MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSAAKSL LKKPDGVKES TESSNTTIED EDVKARKQEI IKVTEQLIEA 370 380 390 400 410 420 INNGDFEAYT KICDPGLTAF EPEALGNLVE GMDFHRFYFE NALPKINKPI HTIILNPHVH 430 440 450 460 470 LVGDDAACIA YIRLTQYMDG NGMPKTMQSE ETRVWHRRDG KWQNIHFHRS GSPTVPIN « Hide
Isoform Delta 6. Checksum: F84B958F92A2BF6C Show »	FASTA	512	57,825
10 20 30 40 50 60 MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSAAKSL LKKPDGVKIN NKANVVTSPK ENIPTPALEP QTTVIHNPDG 370 380 390 400 410 420 NKESTESSNT TIEDEDVKAR KQEIIKVTEQ LIEAINNGDF EAYTKICDPG LTAFEPEALG 430 440 450 460 470 480 NLVEGMDFHR FYFENALPKI NKPIHTIILN PHVHLVGDDA ACIAYIRLTQ YMDGNGMPKT 490 500 510 MQSEETRVWH RRDGKWQNIH FHRSGSPTVP IN « Hide
Isoform Delta 7. Checksum: BC7AC4EE329AC27A Show »	FASTA	498	56,293
10 20 30 40 50 60 MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSAAKSL LKKPDGVKIN NKANVVTSPK ENIPTPALES TESSNTTIED 370 380 390 400 410 420 EDVKARKQEI IKVTEQLIEA INNGDFEAYT KICDPGLTAF EPEALGNLVE GMDFHRFYFE 430 440 450 460 470 480 NALPKINKPI HTIILNPHVH LVGDDAACIA YIRLTQYMDG NGMPKTMQSE ETRVWHRRDG 490 KWQNIHFHRS GSPTVPIN « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tissue-specific expression of four types of rat calmodulin-dependent protein kinase II mRNAs." Tobimatsu T., Fujisawa H. J. Biol. Chem. 264:17907-17912(1989) [PubMed: 2553697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM DELTA 1).
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM DELTA 2). Tissue: Prostate.
[3]	"Identification of novel isoforms of the delta subunit of Ca2+/calmodulin-dependent protein kinase II. Differential expression in rat brain and aorta." Schworer C.M., Rothblum L.I., Thekkumkara T.J., Singer H.A. J. Biol. Chem. 268:14443-14449(1993) [PubMed: 8390994] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 314-533 (ISOFORMS DELTA 2; DELTA 3 AND DELTA 4). Strain: Sprague-Dawley. Tissue: Aorta and Skeletal muscle.
[4]	"New isoforms of multifunctional calcium/calmodulin-dependent protein kinase II." Mayer P., Moehlig M., Schatz H., Pfeiffer A. FEBS Lett. 333:315-318(1993) [PubMed: 8224201] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 307-533 (ISOFORMS DELTA 1; DELTA 2; DELTA 4; DELTA 5; DELTA 6 AND DELTA 7).
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	J05072 mRNA. Translation: AAA40866.1. BC107562 mRNA. Translation: AAI07563.1. L13406 mRNA. Translation: AAA41479.1. L13407 mRNA. Translation: AAA41480.1. L13408 mRNA. Translation: AAA41481.1. X77192 Genomic DNA. Translation: CAA54412.1. X77193 Genomic DNA. Translation: CAA54413.1. X77194 Genomic DNA. Translation: CAA54414.1. X77195 Genomic DNA. Translation: CAA54415.1. X75774 Genomic DNA. Translation: CAA53395.1.
IPI	IPI00212226. IPI00213583. IPI00231612. IPI00231613. IPI00231614. IPI00480684. IPI00480796.
PIR	A34366.
RefSeq	NP_036651.1.
UniGene	Rn.87208
3D structure databases
HSSP	HSSP built from PDB template 1HKX based on UniProtKB P11798.
SMR	P15791. Positions 8-315, 370-508.
ModBase	Search...
PTM databases
PhosphoSite	P15791.
Proteomic databases
PRIDE	P15791.
Genome annotation databases
Ensembl	ENSRNOT00000015457; ENSRNOP00000015457; ENSRNOG00000011589; Rattus norvegicus. [Genome view] ENSRNOT00000016035; ENSRNOP00000016035; ENSRNOG00000011589; Rattus norvegicus. [Genome view]
GeneID	24246.
KEGG	rno:24246.
Organism-specific databases
CTD	24246.
RGD	2263. Camk2d.
Phylogenomic databases
HOVERGEN	P15791.
Enzyme and pathway databases
BRENDA	2.7.11.17. 248.
Gene expression databases
Genevestigator	P15791.
Family and domain databases
InterPro	IPR015742. Ca/calmodulin-dep_kinase. IPR013543. Ca/calmodulin-dep_kinase-assoc. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22982:SF64. CaMKII. 1 hit.
Pfam	PF08332. CaMKII_AD. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	602745.

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Entry information

Entry name

KCC2D_RAT

Accession

Primary (citable) accession number: P15791
Secondary accession number(s): P97915

Q63908

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	October 13, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents