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Protein

Casein kinase II subunit alpha

Gene

CKA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. May play a role in cell proliferation. Required for cell viability.

Miscellaneous

Present with 7180 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69ATPPROSITE-ProRule annotation1
Active sitei195Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 54ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • cellular response to DNA damage stimulus Source: SGD
  • donor selection Source: SGD
  • peptidyl-serine phosphorylation Source: SGD
  • peptidyl-threonine phosphorylation Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of transcription from RNA polymerase III promoter Source: SGD
  • regulation of transcription from RNA polymerase I promoter Source: SGD
  • rRNA methylation Source: Reactome

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31307-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-6790901. rRNA modification in the nucleus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase II subunit alpha (EC:2.7.11.1)
Short name:
CK II subunit alpha
Gene namesi
Name:CKA1
Ordered Locus Names:YIL035C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL035C.
SGDiS000001297. CKA1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858891 – 372Casein kinase II subunit alphaAdd BLAST372

Proteomic databases

MaxQBiP15790.
PRIDEiP15790.

PTM databases

iPTMnetiP15790.

Interactioni

Subunit structurei

Tetramer composed of an alpha chain, an alpha', one beta chain and one beta' chain. Interacts with FACT subunits POB3 and SPT16.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34955. 360 interactors.
DIPiDIP-48N.
IntActiP15790. 142 interactors.
MINTiMINT-583532.
STRINGi4932.YIL035C.

Structurei

Secondary structure

1372
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 12Combined sources3
Helixi15 – 18Combined sources4
Helixi21 – 24Combined sources4
Helixi26 – 28Combined sources3
Beta strandi37 – 47Combined sources11
Beta strandi53 – 59Combined sources7
Turni60 – 62Combined sources3
Beta strandi65 – 70Combined sources6
Helixi76 – 91Combined sources16
Helixi96 – 98Combined sources3
Helixi103 – 106Combined sources4
Helixi112 – 115Combined sources4
Helixi129 – 132Combined sources4
Beta strandi136 – 141Combined sources6
Turni143 – 145Combined sources3
Beta strandi148 – 153Combined sources6
Helixi160 – 163Combined sources4
Helixi164 – 166Combined sources3
Helixi169 – 188Combined sources20
Helixi198 – 200Combined sources3
Beta strandi201 – 204Combined sources4
Helixi205 – 207Combined sources3
Beta strandi209 – 212Combined sources4
Helixi234 – 236Combined sources3
Helixi239 – 242Combined sources4
Helixi251 – 266Combined sources16
Beta strandi269 – 272Combined sources4
Helixi277 – 288Combined sources12
Helixi290 – 300Combined sources11
Helixi306 – 308Combined sources3
Helixi319 – 322Combined sources4
Turni325 – 327Combined sources3
Helixi328 – 331Combined sources4
Helixi334 – 343Combined sources10
Helixi348 – 350Combined sources3
Helixi354 – 358Combined sources5
Helixi361 – 363Combined sources3
Helixi364 – 367Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FI1X-ray2.09A1-372[»]
4JQEX-ray1.77A1-372[»]
4JR7X-ray1.48A1-372[»]
4LFIX-ray1.85A/B1-372[»]
4MWHX-ray2.09A1-372[»]
ProteinModelPortaliP15790.
SMRiP15790.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 363Protein kinasePROSITE-ProRule annotationAdd BLAST324

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00890000140687.
HOGENOMiHOG000233021.
InParanoidiP15790.
KOiK03097.
OMAiNNTDFRS.
OrthoDBiEOG092C3L63.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P15790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCRVWSEAR VYTNINKQRT EEYWDYENTV IDWSTNTKDY EIENKVGRGK
60 70 80 90 100
YSEVFQGVKL DSKVKIVIKM LKPVKKKKIK REIKILTDLS NEKVPPTTLP
110 120 130 140 150
FQKDQYYTNQ KEDVLKFIRP YIFDQPHNGH ANIIHLFDII KDPISKTPAL
160 170 180 190 200
VFEYVDNVDF RILYPKLTDL EIRFYMFELL KALDYCHSMG IMHRDVKPHN
210 220 230 240 250
VMIDHKNKKL RLIDWGLAEF YHVNMEYNVR VASRFFKGPE LLVDYRMYDY
260 270 280 290 300
SLDLWSFGTM LASMIFKREP FFHGTSNTDQ LVKIVKVLGT SDFEKYLLKY
310 320 330 340 350
EITLPREFYD MDQYIRKPWH RFINDGNKHL SGNDEIIDLI DNLLRYDHQE
360 370
RLTAKEAMGH PWFAPIREQI EK
Length:372
Mass (Da):44,668
Last modified:April 1, 1990 - v1
Checksum:i6A1A7F1BF47250C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22473 Genomic DNA. Translation: AAA34534.1.
Z46861 Genomic DNA. Translation: CAA86916.1.
AY558299 Genomic DNA. Translation: AAS56625.1.
X62630 Genomic DNA. Translation: CAA44498.1.
BK006942 Genomic DNA. Translation: DAA08513.1.
PIRiA31564.
RefSeqiNP_012229.1. NM_001179385.1.

Genome annotation databases

EnsemblFungiiYIL035C; YIL035C; YIL035C.
GeneIDi854776.
KEGGisce:YIL035C.

Similar proteinsi

Entry informationi

Entry nameiCSK21_YEAST
AccessioniPrimary (citable) accession number: P15790
Secondary accession number(s): D6VVP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 27, 2017
This is version 176 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names