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P15790

- CSK21_YEAST

UniProt

P15790 - CSK21_YEAST

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Protein

Casein kinase II subunit alpha

Gene

CKA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. May play a role in cell proliferation. Required for cell viability.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691ATPPROSITE-ProRule annotation
Active sitei195 – 1951Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 549ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: SGD
  2. donor selection Source: SGD
  3. protein phosphorylation Source: SGD
  4. regulation of transcription from RNA polymerase III promoter Source: SGD
  5. regulation of transcription from RNA polymerase I promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31307-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase II subunit alpha (EC:2.7.11.1)
Short name:
CK II subunit alpha
Gene namesi
Name:CKA1
Ordered Locus Names:YIL035C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL035c.
SGDiS000001297. CKA1.

Subcellular locationi

GO - Cellular componenti

  1. protein kinase CK2 complex Source: SGD
  2. UTP-C complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Casein kinase II subunit alphaPRO_0000085889Add
BLAST

Proteomic databases

MaxQBiP15790.
PaxDbiP15790.
PeptideAtlasiP15790.

Expressioni

Gene expression databases

GenevestigatoriP15790.

Interactioni

Subunit structurei

Tetramer composed of an alpha chain, an alpha', one beta chain and one beta' chain. Interacts with FACT subunits POB3 and SPT16.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG32P404582EBI-9533,EBI-25256
CDC14Q006843EBI-9533,EBI-4192
CKA2P194548EBI-9533,EBI-9548
CKB1P436397EBI-9533,EBI-9563
CKB2P389307EBI-9533,EBI-9578

Protein-protein interaction databases

BioGridi34955. 258 interactions.
DIPiDIP-48N.
IntActiP15790. 67 interactions.
MINTiMINT-583532.
STRINGi4932.YIL035C.

Structurei

Secondary structure

1
372
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 123
Helixi15 – 184
Helixi21 – 244
Helixi26 – 283
Beta strandi37 – 4711
Beta strandi53 – 597
Turni60 – 623
Beta strandi65 – 706
Helixi76 – 9116
Helixi96 – 983
Helixi103 – 1064
Helixi112 – 1154
Helixi129 – 1324
Beta strandi136 – 1416
Turni143 – 1453
Beta strandi148 – 1536
Helixi160 – 1634
Helixi164 – 1663
Helixi169 – 18820
Helixi198 – 2003
Beta strandi201 – 2044
Helixi205 – 2073
Beta strandi209 – 2124
Helixi234 – 2363
Helixi239 – 2424
Helixi251 – 26616
Beta strandi269 – 2724
Helixi277 – 28812
Helixi290 – 30011
Helixi306 – 3083
Helixi319 – 3224
Turni325 – 3273
Helixi328 – 3314
Helixi334 – 34310
Helixi348 – 3503
Helixi354 – 3585
Helixi361 – 3633
Helixi364 – 3674

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FI1X-ray2.09A1-372[»]
4JQEX-ray1.77A1-372[»]
4JR7X-ray1.48A1-372[»]
4LFIX-ray1.85A/B1-372[»]
4MWHX-ray2.09A1-372[»]
ProteinModelPortaliP15790.
SMRiP15790. Positions 3-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 363324Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00740000116084.
HOGENOMiHOG000233021.
InParanoidiP15790.
KOiK03097.
OMAiLFGYLRK.
OrthoDBiEOG79PK04.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15790-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKCRVWSEAR VYTNINKQRT EEYWDYENTV IDWSTNTKDY EIENKVGRGK
60 70 80 90 100
YSEVFQGVKL DSKVKIVIKM LKPVKKKKIK REIKILTDLS NEKVPPTTLP
110 120 130 140 150
FQKDQYYTNQ KEDVLKFIRP YIFDQPHNGH ANIIHLFDII KDPISKTPAL
160 170 180 190 200
VFEYVDNVDF RILYPKLTDL EIRFYMFELL KALDYCHSMG IMHRDVKPHN
210 220 230 240 250
VMIDHKNKKL RLIDWGLAEF YHVNMEYNVR VASRFFKGPE LLVDYRMYDY
260 270 280 290 300
SLDLWSFGTM LASMIFKREP FFHGTSNTDQ LVKIVKVLGT SDFEKYLLKY
310 320 330 340 350
EITLPREFYD MDQYIRKPWH RFINDGNKHL SGNDEIIDLI DNLLRYDHQE
360 370
RLTAKEAMGH PWFAPIREQI EK
Length:372
Mass (Da):44,668
Last modified:April 1, 1990 - v1
Checksum:i6A1A7F1BF47250C9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22473 Genomic DNA. Translation: AAA34534.1.
Z46861 Genomic DNA. Translation: CAA86916.1.
AY558299 Genomic DNA. Translation: AAS56625.1.
X62630 Genomic DNA. Translation: CAA44498.1.
BK006942 Genomic DNA. Translation: DAA08513.1.
PIRiA31564.
RefSeqiNP_012229.1. NM_001179385.1.

Genome annotation databases

EnsemblFungiiYIL035C; YIL035C; YIL035C.
GeneIDi854776.
KEGGisce:YIL035C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22473 Genomic DNA. Translation: AAA34534.1 .
Z46861 Genomic DNA. Translation: CAA86916.1 .
AY558299 Genomic DNA. Translation: AAS56625.1 .
X62630 Genomic DNA. Translation: CAA44498.1 .
BK006942 Genomic DNA. Translation: DAA08513.1 .
PIRi A31564.
RefSeqi NP_012229.1. NM_001179385.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4FI1 X-ray 2.09 A 1-372 [» ]
4JQE X-ray 1.77 A 1-372 [» ]
4JR7 X-ray 1.48 A 1-372 [» ]
4LFI X-ray 1.85 A/B 1-372 [» ]
4MWH X-ray 2.09 A 1-372 [» ]
ProteinModelPortali P15790.
SMRi P15790. Positions 3-371.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34955. 258 interactions.
DIPi DIP-48N.
IntActi P15790. 67 interactions.
MINTi MINT-583532.
STRINGi 4932.YIL035C.

Proteomic databases

MaxQBi P15790.
PaxDbi P15790.
PeptideAtlasi P15790.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL035C ; YIL035C ; YIL035C .
GeneIDi 854776.
KEGGi sce:YIL035C.

Organism-specific databases

CYGDi YIL035c.
SGDi S000001297. CKA1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00740000116084.
HOGENOMi HOG000233021.
InParanoidi P15790.
KOi K03097.
OMAi LFGYLRK.
OrthoDBi EOG79PK04.

Enzyme and pathway databases

BioCyci YEAST:G3O-31307-MONOMER.
BRENDAi 2.7.11.1. 984.

Miscellaneous databases

NextBioi 977550.

Gene expression databases

Genevestigatori P15790.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 2 hits.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, sequencing, and disruption of the CKA1 gene encoding the alpha subunit of yeast casein kinase II."
    Chen-Wu J.L.-P., Padmanabha R., Glover C.V.C.
    Mol. Cell. Biol. 8:4981-4990(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein."
    Amatruda J.F., Cooper J.A.
    J. Cell Biol. 117:1067-1076(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-142.
  6. "Casein kinase II of yeast contains two distinct alpha polypeptides and an unusually large beta subunit."
    Padmanabha R., Glover C.V.C.
    J. Biol. Chem. 262:1829-1835(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-45.
  7. "RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach."
    Krogan N.J., Kim M., Ahn S.H., Zhong G., Kobor M.S., Cagney G., Emili A., Shilatifard A., Buratowski S., Greenblatt J.F.
    Mol. Cell. Biol. 22:6979-6992(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH POB3 AND SPT16.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCSK21_YEAST
AccessioniPrimary (citable) accession number: P15790
Secondary accession number(s): D6VVP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 29, 2014
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7180 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3