P15790 (CSK21_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Casein kinase II subunit alpha
Short name=CK II subunit alpha
EC=2.7.11.1

Gene names

Name:	CKA1
Ordered Locus Names:	YIL035C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. May play a role in cell proliferation. Required for cell viability.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Tetramer composed of an alpha chain, an alpha', one beta chain and one beta' chain. Interacts with FACT subunits POB3 and SPT16. Ref.7
Miscellaneous	Present with 7180 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	donor selection Inferred from genetic interaction PubMed 22496671. Source: SGD regulation of transcription from RNA polymerase I promoter Inferred from direct assay PubMed 11551505. Source: SGD regulation of transcription from RNA polymerase III promoter Inferred from direct assay PubMed 11551505. Source: SGD response to DNA damage stimulus Inferred from direct assay PubMed 11551505. Source: SGD
Cellular_component	UTP-C complex Inferred from direct assay PubMed 17515605. Source: SGD protein kinase CK2 complex Inferred from direct assay PubMed 8135547. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from direct assay PubMed 8226802. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC14	Q00684	3	EBI-9533,EBI-4192
CKA2	P19454	10	EBI-9533,EBI-9548
CKB1	P43639	9	EBI-9533,EBI-9563
CKB2	P38930	9	EBI-9533,EBI-9578

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Casein kinase II subunit alpha		PRO_0000085889
Regions
	Domain	40 – 363	324	Protein kinase
	Nucleotide binding	46 – 54	9	ATP By similarity
Sites
	Active site	195	1	Proton acceptor By similarity
	Binding site	69	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15790 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 6A1A7F1BF47250C9	FASTA	372	44,668
	10 20 30 40 50 60 MKCRVWSEAR VYTNINKQRT EEYWDYENTV IDWSTNTKDY EIENKVGRGK YSEVFQGVKL 70 80 90 100 110 120 DSKVKIVIKM LKPVKKKKIK REIKILTDLS NEKVPPTTLP FQKDQYYTNQ KEDVLKFIRP 130 140 150 160 170 180 YIFDQPHNGH ANIIHLFDII KDPISKTPAL VFEYVDNVDF RILYPKLTDL EIRFYMFELL 190 200 210 220 230 240 KALDYCHSMG IMHRDVKPHN VMIDHKNKKL RLIDWGLAEF YHVNMEYNVR VASRFFKGPE 250 260 270 280 290 300 LLVDYRMYDY SLDLWSFGTM LASMIFKREP FFHGTSNTDQ LVKIVKVLGT SDFEKYLLKY 310 320 330 340 350 360 EITLPREFYD MDQYIRKPWH RFINDGNKHL SGNDEIIDLI DNLLRYDHQE RLTAKEAMGH 370 PWFAPIREQI EK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, sequencing, and disruption of the CKA1 gene encoding the alpha subunit of yeast casein kinase II." Chen-Wu J.L.-P., Padmanabha R., Glover C.V.C. Mol. Cell. Biol. 8:4981-4990(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein." Amatruda J.F., Cooper J.A. J. Cell Biol. 117:1067-1076(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-142.
[6]	"Casein kinase II of yeast contains two distinct alpha polypeptides and an unusually large beta subunit." Padmanabha R., Glover C.V.C. J. Biol. Chem. 262:1829-1835(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-45.
[7]	"RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach." Krogan N.J., Kim M., Ahn S.H., Zhong G., Kobor M.S., Cagney G., Emili A., Shilatifard A., Buratowski S., Greenblatt J.F. Mol. Cell. Biol. 22:6979-6992(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH POB3 AND SPT16.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22473 Genomic DNA. Translation: AAA34534.1. Z46861 Genomic DNA. Translation: CAA86916.1. AY558299 Genomic DNA. Translation: AAS56625.1. X62630 Genomic DNA. Translation: CAA44498.1. BK006942 Genomic DNA. Translation: DAA08513.1.
PIR	A31564.
RefSeq	NP_012229.1. NM_001179385.1.
3D structure databases
ProteinModelPortal	P15790.
SMR	P15790. Positions 7-369.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48N.
IntAct	P15790. 107 interactions.
MINT	MINT-583532.
STRING	4932.YIL035C.
Proteomic databases
PaxDb	P15790.
PeptideAtlas	P15790.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL035C; YIL035C; YIL035C.
GeneID	854776.
KEGG	sce:YIL035C.
Organism-specific databases
CYGD	YIL035c.
SGD	S000001297. CKA1.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00390000004215.
HOGENOM	HOG000233021.
KO	K03097.
OMA	FGYLRKY.
OrthoDB	EOG4QVGMQ.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31307-MONOMER.
BRENDA	2.7.11.1. 984.
Gene expression databases
Genevestigator	P15790.
GermOnline	YIL035C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977550.

Entry information

Entry name

CSK21_YEAST

Accession

Primary (citable) accession number: P15790
Secondary accession number(s): D6VVP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 29, 2013
This is version 139 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program