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P15790

- CSK21_YEAST

UniProt

P15790 - CSK21_YEAST

Protein

Casein kinase II subunit alpha

Gene

CKA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. May play a role in cell proliferation. Required for cell viability.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691ATPPROSITE-ProRule annotation
    Active sitei195 – 1951Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi46 – 549ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine kinase activity Source: SGD

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: SGD
    2. donor selection Source: SGD
    3. protein phosphorylation Source: SGD
    4. regulation of transcription from RNA polymerase III promoter Source: SGD
    5. regulation of transcription from RNA polymerase I promoter Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31307-MONOMER.
    BRENDAi2.7.11.1. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Casein kinase II subunit alpha (EC:2.7.11.1)
    Short name:
    CK II subunit alpha
    Gene namesi
    Name:CKA1
    Ordered Locus Names:YIL035C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL035c.
    SGDiS000001297. CKA1.

    Subcellular locationi

    GO - Cellular componenti

    1. protein kinase CK2 complex Source: SGD
    2. UTP-C complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372Casein kinase II subunit alphaPRO_0000085889Add
    BLAST

    Proteomic databases

    MaxQBiP15790.
    PaxDbiP15790.
    PeptideAtlasiP15790.

    Expressioni

    Gene expression databases

    GenevestigatoriP15790.

    Interactioni

    Subunit structurei

    Tetramer composed of an alpha chain, an alpha', one beta chain and one beta' chain. Interacts with FACT subunits POB3 and SPT16.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ATG32P404582EBI-9533,EBI-25256
    CDC14Q006843EBI-9533,EBI-4192
    CKA2P194548EBI-9533,EBI-9548
    CKB1P436397EBI-9533,EBI-9563
    CKB2P389307EBI-9533,EBI-9578

    Protein-protein interaction databases

    BioGridi34955. 257 interactions.
    DIPiDIP-48N.
    IntActiP15790. 67 interactions.
    MINTiMINT-583532.
    STRINGi4932.YIL035C.

    Structurei

    Secondary structure

    1
    372
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 123
    Helixi15 – 184
    Helixi21 – 244
    Helixi26 – 283
    Beta strandi37 – 4711
    Beta strandi53 – 597
    Turni60 – 623
    Beta strandi65 – 706
    Helixi76 – 9116
    Helixi96 – 983
    Helixi103 – 1064
    Helixi112 – 1154
    Helixi129 – 1324
    Beta strandi136 – 1416
    Turni143 – 1453
    Beta strandi148 – 1536
    Helixi160 – 1634
    Helixi164 – 1663
    Helixi169 – 18820
    Helixi198 – 2003
    Beta strandi201 – 2044
    Helixi205 – 2073
    Beta strandi209 – 2124
    Helixi234 – 2363
    Helixi239 – 2424
    Helixi251 – 26616
    Beta strandi269 – 2724
    Helixi277 – 28812
    Helixi290 – 30011
    Helixi306 – 3083
    Helixi319 – 3224
    Turni325 – 3273
    Helixi328 – 3314
    Helixi334 – 34310
    Helixi348 – 3503
    Helixi354 – 3585
    Helixi361 – 3633
    Helixi364 – 3674

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4FI1X-ray2.09A1-372[»]
    4JQEX-ray1.77A1-372[»]
    4JR7X-ray1.48A1-372[»]
    4LFIX-ray1.85A/B1-372[»]
    4MWHX-ray2.09A1-372[»]
    ProteinModelPortaliP15790.
    SMRiP15790. Positions 3-371.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 363324Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000116084.
    HOGENOMiHOG000233021.
    KOiK03097.
    OMAiLFGYLRK.
    OrthoDBiEOG79PK04.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 2 hits.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15790-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKCRVWSEAR VYTNINKQRT EEYWDYENTV IDWSTNTKDY EIENKVGRGK    50
    YSEVFQGVKL DSKVKIVIKM LKPVKKKKIK REIKILTDLS NEKVPPTTLP 100
    FQKDQYYTNQ KEDVLKFIRP YIFDQPHNGH ANIIHLFDII KDPISKTPAL 150
    VFEYVDNVDF RILYPKLTDL EIRFYMFELL KALDYCHSMG IMHRDVKPHN 200
    VMIDHKNKKL RLIDWGLAEF YHVNMEYNVR VASRFFKGPE LLVDYRMYDY 250
    SLDLWSFGTM LASMIFKREP FFHGTSNTDQ LVKIVKVLGT SDFEKYLLKY 300
    EITLPREFYD MDQYIRKPWH RFINDGNKHL SGNDEIIDLI DNLLRYDHQE 350
    RLTAKEAMGH PWFAPIREQI EK 372
    Length:372
    Mass (Da):44,668
    Last modified:April 1, 1990 - v1
    Checksum:i6A1A7F1BF47250C9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22473 Genomic DNA. Translation: AAA34534.1.
    Z46861 Genomic DNA. Translation: CAA86916.1.
    AY558299 Genomic DNA. Translation: AAS56625.1.
    X62630 Genomic DNA. Translation: CAA44498.1.
    BK006942 Genomic DNA. Translation: DAA08513.1.
    PIRiA31564.
    RefSeqiNP_012229.1. NM_001179385.1.

    Genome annotation databases

    EnsemblFungiiYIL035C; YIL035C; YIL035C.
    GeneIDi854776.
    KEGGisce:YIL035C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22473 Genomic DNA. Translation: AAA34534.1 .
    Z46861 Genomic DNA. Translation: CAA86916.1 .
    AY558299 Genomic DNA. Translation: AAS56625.1 .
    X62630 Genomic DNA. Translation: CAA44498.1 .
    BK006942 Genomic DNA. Translation: DAA08513.1 .
    PIRi A31564.
    RefSeqi NP_012229.1. NM_001179385.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4FI1 X-ray 2.09 A 1-372 [» ]
    4JQE X-ray 1.77 A 1-372 [» ]
    4JR7 X-ray 1.48 A 1-372 [» ]
    4LFI X-ray 1.85 A/B 1-372 [» ]
    4MWH X-ray 2.09 A 1-372 [» ]
    ProteinModelPortali P15790.
    SMRi P15790. Positions 3-371.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34955. 257 interactions.
    DIPi DIP-48N.
    IntActi P15790. 67 interactions.
    MINTi MINT-583532.
    STRINGi 4932.YIL035C.

    Proteomic databases

    MaxQBi P15790.
    PaxDbi P15790.
    PeptideAtlasi P15790.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL035C ; YIL035C ; YIL035C .
    GeneIDi 854776.
    KEGGi sce:YIL035C.

    Organism-specific databases

    CYGDi YIL035c.
    SGDi S000001297. CKA1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000116084.
    HOGENOMi HOG000233021.
    KOi K03097.
    OMAi LFGYLRK.
    OrthoDBi EOG79PK04.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31307-MONOMER.
    BRENDAi 2.7.11.1. 984.

    Miscellaneous databases

    NextBioi 977550.

    Gene expression databases

    Genevestigatori P15790.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 2 hits.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, sequencing, and disruption of the CKA1 gene encoding the alpha subunit of yeast casein kinase II."
      Chen-Wu J.L.-P., Padmanabha R., Glover C.V.C.
      Mol. Cell. Biol. 8:4981-4990(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein."
      Amatruda J.F., Cooper J.A.
      J. Cell Biol. 117:1067-1076(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-142.
    6. "Casein kinase II of yeast contains two distinct alpha polypeptides and an unusually large beta subunit."
      Padmanabha R., Glover C.V.C.
      J. Biol. Chem. 262:1829-1835(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-45.
    7. "RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach."
      Krogan N.J., Kim M., Ahn S.H., Zhong G., Kobor M.S., Cagney G., Emili A., Shilatifard A., Buratowski S., Greenblatt J.F.
      Mol. Cell. Biol. 22:6979-6992(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH POB3 AND SPT16.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCSK21_YEAST
    AccessioniPrimary (citable) accession number: P15790
    Secondary accession number(s): D6VVP7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 149 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7180 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3