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P15790 (CSK21_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Casein kinase II subunit alpha

Short name=CK II subunit alpha
EC=2.7.11.1
Gene names
Name:CKA1
Ordered Locus Names:YIL035C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. May play a role in cell proliferation. Required for cell viability.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Tetramer composed of an alpha chain, an alpha', one beta chain and one beta' chain. Interacts with FACT subunits POB3 and SPT16. Ref.7

Miscellaneous

Present with 7180 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Casein kinase II subunit alpha
PRO_0000085889

Regions

Domain40 – 363324Protein kinase
Nucleotide binding46 – 549ATP By similarity

Sites

Active site1951Proton acceptor By similarity
Binding site691ATP By similarity

Secondary structure

...................................................................... 372
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P15790 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 6A1A7F1BF47250C9

FASTA37244,668
        10         20         30         40         50         60 
MKCRVWSEAR VYTNINKQRT EEYWDYENTV IDWSTNTKDY EIENKVGRGK YSEVFQGVKL 

        70         80         90        100        110        120 
DSKVKIVIKM LKPVKKKKIK REIKILTDLS NEKVPPTTLP FQKDQYYTNQ KEDVLKFIRP 

       130        140        150        160        170        180 
YIFDQPHNGH ANIIHLFDII KDPISKTPAL VFEYVDNVDF RILYPKLTDL EIRFYMFELL 

       190        200        210        220        230        240 
KALDYCHSMG IMHRDVKPHN VMIDHKNKKL RLIDWGLAEF YHVNMEYNVR VASRFFKGPE 

       250        260        270        280        290        300 
LLVDYRMYDY SLDLWSFGTM LASMIFKREP FFHGTSNTDQ LVKIVKVLGT SDFEKYLLKY 

       310        320        330        340        350        360 
EITLPREFYD MDQYIRKPWH RFINDGNKHL SGNDEIIDLI DNLLRYDHQE RLTAKEAMGH 

       370 
PWFAPIREQI EK 

« Hide

References

« Hide 'large scale' references
[1]"Isolation, sequencing, and disruption of the CKA1 gene encoding the alpha subunit of yeast casein kinase II."
Chen-Wu J.L.-P., Padmanabha R., Glover C.V.C.
Mol. Cell. Biol. 8:4981-4990(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein."
Amatruda J.F., Cooper J.A.
J. Cell Biol. 117:1067-1076(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-142.
[6]"Casein kinase II of yeast contains two distinct alpha polypeptides and an unusually large beta subunit."
Padmanabha R., Glover C.V.C.
J. Biol. Chem. 262:1829-1835(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-45.
[7]"RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach."
Krogan N.J., Kim M., Ahn S.H., Zhong G., Kobor M.S., Cagney G., Emili A., Shilatifard A., Buratowski S., Greenblatt J.F.
Mol. Cell. Biol. 22:6979-6992(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH POB3 AND SPT16.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22473 Genomic DNA. Translation: AAA34534.1.
Z46861 Genomic DNA. Translation: CAA86916.1.
AY558299 Genomic DNA. Translation: AAS56625.1.
X62630 Genomic DNA. Translation: CAA44498.1.
BK006942 Genomic DNA. Translation: DAA08513.1.
PIRA31564.
RefSeqNP_012229.1. NM_001179385.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4FI1X-ray2.09A1-372[»]
4JQEX-ray1.77A1-372[»]
4JR7X-ray1.48A1-372[»]
4LFIX-ray1.85A/B1-372[»]
4MWHX-ray2.09A1-372[»]
ProteinModelPortalP15790.
SMRP15790. Positions 1-371.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34955. 256 interactions.
DIPDIP-48N.
IntActP15790. 67 interactions.
MINTMINT-583532.
STRING4932.YIL035C.

Proteomic databases

PaxDbP15790.
PeptideAtlasP15790.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL035C; YIL035C; YIL035C.
GeneID854776.
KEGGsce:YIL035C.

Organism-specific databases

CYGDYIL035c.
SGDS000001297. CKA1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00740000116084.
HOGENOMHOG000233021.
KOK03097.
OMALFGYLRK.
OrthoDBEOG79PK04.

Enzyme and pathway databases

BioCycYEAST:G3O-31307-MONOMER.
BRENDA2.7.11.1. 984.

Gene expression databases

GenevestigatorP15790.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 2 hits.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977550.

Entry information

Entry nameCSK21_YEAST
AccessionPrimary (citable) accession number: P15790
Secondary accession number(s): D6VVP7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references