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P15781

- PSPB_BOVIN

UniProt

P15781 - PSPB_BOVIN

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Protein

Pulmonary surfactant-associated protein B

Gene

SFTPB

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

GO - Biological processi

  1. respiratory gaseous exchange Source: UniProtKB-KW
  2. sphingolipid metabolic process Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Gaseous exchange

Names & Taxonomyi

Protein namesi
Recommended name:
Pulmonary surfactant-associated protein B
Short name:
SP-B
Alternative name(s):
6 kDa protein
Pulmonary surfactant-associated proteolipid SPL(Phe)
Gene namesi
Name:SFTPB
Synonyms:SFTP3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 11

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
  2. lysosome Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted, Surface film

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Propeptidei23 – 187165PRO_0000285124Add
BLAST
Chaini188 – 26679Pulmonary surfactant-associated protein BPRO_0000175240Add
BLAST
Propeptidei267 – 373107PRO_0000285125Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi68 ↔ 142PROSITE-ProRule annotation
Disulfide bondi71 ↔ 136PROSITE-ProRule annotation
Glycosylationi73 – 731N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi99 ↔ 111PROSITE-ProRule annotation
Disulfide bondi195 ↔ 264PROSITE-ProRule annotation
Disulfide bondi198 ↔ 258PROSITE-ProRule annotation
Disulfide bondi222 ↔ 233PROSITE-ProRule annotation
Disulfide bondi235 – 235InterchainPROSITE-ProRule annotation
Disulfide bondi291 ↔ 358PROSITE-ProRule annotation
Disulfide bondi294 ↔ 352PROSITE-ProRule annotation
Glycosylationi303 – 3031N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi317 ↔ 327PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042549.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IL7model-A188-266[»]
2IP3model-A188-266[»]
ProteinModelPortaliP15781.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 6441Saposin A-typePROSITE-ProRule annotationAdd
BLAST
Domaini64 – 14683Saposin B-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini191 – 26878Saposin B-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini287 – 36276Saposin B-type 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 saposin A-type domain.PROSITE-ProRule annotation
Contains 3 saposin B-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG286450.
GeneTreeiENSGT00530000063434.
HOGENOMiHOG000115745.
HOVERGENiHBG006905.
InParanoidiP15781.
OMAiCWLCRTL.
OrthoDBiEOG7F24TB.
TreeFamiTF316942.

Family and domain databases

Gene3Di1.10.225.10. 3 hits.
InterProiIPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]
PfamiPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 3 hits.
[Graphical view]
PRINTSiPR01797. SAPOSIN.
SMARTiSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 2 hits.
PROSITEiPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15781-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKSHLLPWL LLLPILCGPG TAAAITYSLA CAQGPEFWCQ SLEQALQCRA
60 70 80 90 100
LGHCLQEVWG HVEADDLCQE CENISRLLTK MAKEAIFQDS VRKFLEQECD
110 120 130 140 150
VLPLKLLAPL CRHLLDTYFP LIIEHFQSHM NPKFICQHVG LCKPRHPEPG
160 170 180 190 200
KGPEPWGPLL DKLALPLLPG VPQAKPGPQT QDLSEQLFPI PIPYCWLCRT
210 220 230 240 250
LIKRIQAVIP KGVLAMTVAQ VCHVVPLLVG GICQCLVERY SVILLDTLLG
260 270 280 290 300
RMLPQLVCGL VLRCSSEDSA GPALPALGSV PGEWLPQDSD CQLCMFVTTQ
310 320 330 340 350
AGNSSEQATP QAMRQACLGT WLDRQKCERF VEENAPRLQT LVSSGWDAHM
360 370
ACQALGTCAA PFSPLQCVHS PHF
Length:373
Mass (Da):41,060
Last modified:May 1, 2007 - v3
Checksum:iA41A31AE5A5C0F82
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 1981C → L AA sequence (PubMed:3689402)Curated
Sequence conflicti204 – 2041R → K AA sequence (PubMed:3689402)Curated
Sequence conflicti233 – 2397CQCLVER → IQQLVIE AA sequence (PubMed:3689402)Curated
Sequence conflicti245 – 2451L → LT AA sequence (PubMed:3689402)Curated
Sequence conflicti252 – 2521Missing AA sequence (PubMed:3689402)Curated
Sequence conflicti255 – 2551Q → N AA sequence (PubMed:3689402)Curated
Sequence conflicti261 – 2611V → R AA sequence (PubMed:3689402)Curated
Sequence conflicti266 – 2661S → G AA sequence (PubMed:3689402)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC118395 mRNA. Translation: AAI18396.1.
PIRiA29667.
RefSeqiNP_001068779.1. NM_001075311.2.
XP_005212817.1. XM_005212760.1.
UniGeneiBt.42696.

Genome annotation databases

EnsembliENSBTAT00000045134; ENSBTAP00000042549; ENSBTAG00000021230.
GeneIDi507398.
KEGGibta:507398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC118395 mRNA. Translation: AAI18396.1 .
PIRi A29667.
RefSeqi NP_001068779.1. NM_001075311.2.
XP_005212817.1. XM_005212760.1.
UniGenei Bt.42696.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2IL7 model - A 188-266 [» ]
2IP3 model - A 188-266 [» ]
ProteinModelPortali P15781.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000042549.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000045134 ; ENSBTAP00000042549 ; ENSBTAG00000021230 .
GeneIDi 507398.
KEGGi bta:507398.

Organism-specific databases

CTDi 6439.

Phylogenomic databases

eggNOGi NOG286450.
GeneTreei ENSGT00530000063434.
HOGENOMi HOG000115745.
HOVERGENi HBG006905.
InParanoidi P15781.
OMAi CWLCRTL.
OrthoDBi EOG7F24TB.
TreeFami TF316942.

Miscellaneous databases

NextBioi 20868039.

Family and domain databases

Gene3Di 1.10.225.10. 3 hits.
InterProi IPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view ]
Pfami PF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 3 hits.
[Graphical view ]
PRINTSi PR01797. SAPOSIN.
SMARTi SM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view ]
SUPFAMi SSF47862. SSF47862. 2 hits.
PROSITEi PS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal lung.
  2. "Protein sequence analysis studies on the low molecular weight hydrophobic proteins associated with bovine pulmonary surfactant."
    Olafson R.W., Rink U., Kielland S., Yu S.-H., Chung J., Harding P.G.R., Possmayer F.
    Biochem. Biophys. Res. Commun. 148:1406-1411(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 188-266.
  3. "Characterization of bovine surfactant proteins B and C by electrospray ionization mass spectrometry."
    Liu S., Zhao L., Manzanares D., Doherty-Kirby A., Zhang C., Possmayer F., Lajoie G.A.
    Rapid Commun. Mass Spectrom. 22:197-203(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 188-266, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Characterization of the small hydrophobic proteins associated with pulmonary surfactant."
    Yu S.-H., Chung W., Olafson R.W., Harding P.G.R., Possmayer F.
    Biochim. Biophys. Acta 921:437-448(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 188-197.

Entry informationi

Entry nameiPSPB_BOVIN
AccessioniPrimary (citable) accession number: P15781
Secondary accession number(s): Q148E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: May 1, 2007
Last modified: October 29, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3