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Protein

Pulmonary surfactant-associated protein B

Gene

SFTPB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Gaseous exchange

Enzyme and pathway databases

ReactomeiR-BTA-5683826. Surfactant metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Pulmonary surfactant-associated protein B
Short name:
SP-B
Alternative name(s):
6 kDa protein
Pulmonary surfactant-associated proteolipid SPL(Phe)
Gene namesi
Name:SFTPB
Synonyms:SFTP3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted, Surface film

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000028512423 – 187Add BLAST165
ChainiPRO_0000175240188 – 266Pulmonary surfactant-associated protein BAdd BLAST79
PropeptideiPRO_0000285125267 – 373Add BLAST107

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi68 ↔ 142PROSITE-ProRule annotation
Disulfide bondi71 ↔ 136PROSITE-ProRule annotation
Glycosylationi73N-linked (GlcNAc...)PROSITE-ProRule annotation1
Disulfide bondi99 ↔ 111PROSITE-ProRule annotation
Disulfide bondi195 ↔ 264PROSITE-ProRule annotation
Disulfide bondi198 ↔ 258PROSITE-ProRule annotation
Disulfide bondi222 ↔ 233PROSITE-ProRule annotation
Disulfide bondi235InterchainPROSITE-ProRule annotation
Disulfide bondi291 ↔ 358PROSITE-ProRule annotation
Disulfide bondi294 ↔ 352PROSITE-ProRule annotation
Glycosylationi303N-linked (GlcNAc...)PROSITE-ProRule annotation1
Disulfide bondi317 ↔ 327PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP15781.
PRIDEiP15781.

Expressioni

Gene expression databases

BgeeiENSBTAG00000021230.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042549.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IL7model-A188-266[»]
2IP3model-A188-266[»]
ProteinModelPortaliP15781.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 64Saposin A-typePROSITE-ProRule annotationAdd BLAST41
Domaini64 – 146Saposin B-type 1PROSITE-ProRule annotationAdd BLAST83
Domaini191 – 268Saposin B-type 2PROSITE-ProRule annotationAdd BLAST78
Domaini287 – 362Saposin B-type 3PROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Contains 1 saposin A-type domain.PROSITE-ProRule annotation
Contains 3 saposin B-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1340. Eukaryota.
ENOG410XSI5. LUCA.
GeneTreeiENSGT00530000063434.
HOGENOMiHOG000115745.
HOVERGENiHBG006905.
InParanoidiP15781.
OMAiVWGHVGA.
OrthoDBiEOG091G0K86.
TreeFamiTF316942.

Family and domain databases

Gene3Di1.10.225.10. 3 hits.
InterProiIPR003119. SAP_A.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PfamiPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 2 hits.
[Graphical view]
PRINTSiPR01797. SAPOSIN.
SMARTiSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 2 hits.
PROSITEiPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15781-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSHLLPWL LLLPILCGPG TAAAITYSLA CAQGPEFWCQ SLEQALQCRA
60 70 80 90 100
LGHCLQEVWG HVEADDLCQE CENISRLLTK MAKEAIFQDS VRKFLEQECD
110 120 130 140 150
VLPLKLLAPL CRHLLDTYFP LIIEHFQSHM NPKFICQHVG LCKPRHPEPG
160 170 180 190 200
KGPEPWGPLL DKLALPLLPG VPQAKPGPQT QDLSEQLFPI PIPYCWLCRT
210 220 230 240 250
LIKRIQAVIP KGVLAMTVAQ VCHVVPLLVG GICQCLVERY SVILLDTLLG
260 270 280 290 300
RMLPQLVCGL VLRCSSEDSA GPALPALGSV PGEWLPQDSD CQLCMFVTTQ
310 320 330 340 350
AGNSSEQATP QAMRQACLGT WLDRQKCERF VEENAPRLQT LVSSGWDAHM
360 370
ACQALGTCAA PFSPLQCVHS PHF
Length:373
Mass (Da):41,060
Last modified:May 1, 2007 - v3
Checksum:iA41A31AE5A5C0F82
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198C → L AA sequence (PubMed:3689402).Curated1
Sequence conflicti204R → K AA sequence (PubMed:3689402).Curated1
Sequence conflicti233 – 239CQCLVER → IQQLVIE AA sequence (PubMed:3689402).Curated7
Sequence conflicti245L → LT AA sequence (PubMed:3689402).Curated1
Sequence conflicti252Missing AA sequence (PubMed:3689402).Curated1
Sequence conflicti255Q → N AA sequence (PubMed:3689402).Curated1
Sequence conflicti261V → R AA sequence (PubMed:3689402).Curated1
Sequence conflicti266S → G AA sequence (PubMed:3689402).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC118395 mRNA. Translation: AAI18396.1.
PIRiA29667.
RefSeqiNP_001068779.1. NM_001075311.2.
XP_005212817.1. XM_005212760.2.
UniGeneiBt.42696.

Genome annotation databases

EnsembliENSBTAT00000045134; ENSBTAP00000042549; ENSBTAG00000021230.
GeneIDi507398.
KEGGibta:507398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC118395 mRNA. Translation: AAI18396.1.
PIRiA29667.
RefSeqiNP_001068779.1. NM_001075311.2.
XP_005212817.1. XM_005212760.2.
UniGeneiBt.42696.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IL7model-A188-266[»]
2IP3model-A188-266[»]
ProteinModelPortaliP15781.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042549.

Proteomic databases

PaxDbiP15781.
PRIDEiP15781.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000045134; ENSBTAP00000042549; ENSBTAG00000021230.
GeneIDi507398.
KEGGibta:507398.

Organism-specific databases

CTDi6439.

Phylogenomic databases

eggNOGiKOG1340. Eukaryota.
ENOG410XSI5. LUCA.
GeneTreeiENSGT00530000063434.
HOGENOMiHOG000115745.
HOVERGENiHBG006905.
InParanoidiP15781.
OMAiVWGHVGA.
OrthoDBiEOG091G0K86.
TreeFamiTF316942.

Enzyme and pathway databases

ReactomeiR-BTA-5683826. Surfactant metabolism.

Gene expression databases

BgeeiENSBTAG00000021230.

Family and domain databases

Gene3Di1.10.225.10. 3 hits.
InterProiIPR003119. SAP_A.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PfamiPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 2 hits.
[Graphical view]
PRINTSiPR01797. SAPOSIN.
SMARTiSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 2 hits.
PROSITEiPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSPB_BOVIN
AccessioniPrimary (citable) accession number: P15781
Secondary accession number(s): Q148E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: May 1, 2007
Last modified: November 2, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.