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P15781 (PSPB_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pulmonary surfactant-associated protein B

Short name=SP-B
Alternative name(s):
6 kDa protein
Pulmonary surfactant-associated proteolipid SPL(Phe)
Gene names
Name:SFTPB
Synonyms:SFTP3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

Subunit structure

Homodimer; disulfide-linked.

Subcellular location

Secretedextracellular spacesurface film.

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Sequence similarities

Contains 1 saposin A-type domain.

Contains 3 saposin B-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Propeptide23 – 187165
PRO_0000285124
Chain188 – 26679Pulmonary surfactant-associated protein B
PRO_0000175240
Propeptide267 – 373107
PRO_0000285125

Regions

Domain24 – 6441Saposin A-type
Domain64 – 14683Saposin B-type 1
Domain191 – 26878Saposin B-type 2
Domain287 – 36276Saposin B-type 3

Amino acid modifications

Glycosylation731N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Disulfide bond68 ↔ 142 By similarity
Disulfide bond71 ↔ 136 By similarity
Disulfide bond99 ↔ 111 By similarity
Disulfide bond195 ↔ 264 By similarity
Disulfide bond198 ↔ 258 By similarity
Disulfide bond222 ↔ 233 By similarity
Disulfide bond235Interchain By similarity
Disulfide bond291 ↔ 358 By similarity
Disulfide bond294 ↔ 352 By similarity
Disulfide bond317 ↔ 327 By similarity

Experimental info

Sequence conflict1981C → L AA sequence Ref.2
Sequence conflict2041R → K AA sequence Ref.2
Sequence conflict233 – 2397CQCLVER → IQQLVIE AA sequence Ref.2
Sequence conflict2451L → LT AA sequence Ref.2
Sequence conflict2521Missing AA sequence Ref.2
Sequence conflict2551Q → N AA sequence Ref.2
Sequence conflict2611V → R AA sequence Ref.2
Sequence conflict2661S → G AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P15781 [UniParc].

Last modified May 1, 2007. Version 3.
Checksum: A41A31AE5A5C0F82

FASTA37341,060
        10         20         30         40         50         60 
MAKSHLLPWL LLLPILCGPG TAAAITYSLA CAQGPEFWCQ SLEQALQCRA LGHCLQEVWG 

        70         80         90        100        110        120 
HVEADDLCQE CENISRLLTK MAKEAIFQDS VRKFLEQECD VLPLKLLAPL CRHLLDTYFP 

       130        140        150        160        170        180 
LIIEHFQSHM NPKFICQHVG LCKPRHPEPG KGPEPWGPLL DKLALPLLPG VPQAKPGPQT 

       190        200        210        220        230        240 
QDLSEQLFPI PIPYCWLCRT LIKRIQAVIP KGVLAMTVAQ VCHVVPLLVG GICQCLVERY 

       250        260        270        280        290        300 
SVILLDTLLG RMLPQLVCGL VLRCSSEDSA GPALPALGSV PGEWLPQDSD CQLCMFVTTQ 

       310        320        330        340        350        360 
AGNSSEQATP QAMRQACLGT WLDRQKCERF VEENAPRLQT LVSSGWDAHM ACQALGTCAA 

       370 
PFSPLQCVHS PHF 

« Hide

References

« Hide 'large scale' references
[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal lung.
[2]"Protein sequence analysis studies on the low molecular weight hydrophobic proteins associated with bovine pulmonary surfactant."
Olafson R.W., Rink U., Kielland S., Yu S.-H., Chung J., Harding P.G.R., Possmayer F.
Biochem. Biophys. Res. Commun. 148:1406-1411(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 188-266.
[3]"Characterization of bovine surfactant proteins B and C by electrospray ionization mass spectrometry."
Liu S., Zhao L., Manzanares D., Doherty-Kirby A., Zhang C., Possmayer F., Lajoie G.A.
Rapid Commun. Mass Spectrom. 22:197-203(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 188-266, IDENTIFICATION BY MASS SPECTROMETRY.
[4]"Characterization of the small hydrophobic proteins associated with pulmonary surfactant."
Yu S.-H., Chung W., Olafson R.W., Harding P.G.R., Possmayer F.
Biochim. Biophys. Acta 921:437-448(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 188-197.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC118395 mRNA. Translation: AAI18396.1.
PIRA29667.
RefSeqNP_001068779.1. NM_001075311.2.
XP_005212817.1. XM_005212760.1.
UniGeneBt.42696.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2IL7model-A188-266[»]
2IP3model-A188-266[»]
ProteinModelPortalP15781.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000042549.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000045134; ENSBTAP00000042549; ENSBTAG00000021230.
GeneID507398.
KEGGbta:507398.

Organism-specific databases

CTD6439.

Phylogenomic databases

eggNOGNOG286450.
GeneTreeENSGT00530000063434.
HOGENOMHOG000115745.
HOVERGENHBG006905.
InParanoidP15781.
OMACWLCRTL.
OrthoDBEOG7F24TB.
TreeFamTF316942.

Family and domain databases

Gene3D1.10.225.10. 3 hits.
InterProIPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]
PfamPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 3 hits.
[Graphical view]
PRINTSPR01797. SAPOSIN.
SMARTSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMSSF47862. SSF47862. 2 hits.
PROSITEPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20868039.

Entry information

Entry namePSPB_BOVIN
AccessionPrimary (citable) accession number: P15781
Secondary accession number(s): Q148E8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: May 1, 2007
Last modified: April 16, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references