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P15781

- PSPB_BOVIN

UniProt

P15781 - PSPB_BOVIN

Protein

Pulmonary surfactant-associated protein B

Gene

SFTPB

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 3 (01 May 2007)
      Previous versions | rss
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    Functioni

    Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

    GO - Biological processi

    1. respiratory gaseous exchange Source: UniProtKB-KW
    2. sphingolipid metabolic process Source: InterPro

    Keywords - Biological processi

    Gaseous exchange

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pulmonary surfactant-associated protein B
    Short name:
    SP-B
    Alternative name(s):
    6 kDa protein
    Pulmonary surfactant-associated proteolipid SPL(Phe)
    Gene namesi
    Name:SFTPB
    Synonyms:SFTP3
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 11

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell
    2. lysosome Source: InterPro

    Keywords - Cellular componenti

    Secreted, Surface film

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Propeptidei23 – 187165PRO_0000285124Add
    BLAST
    Chaini188 – 26679Pulmonary surfactant-associated protein BPRO_0000175240Add
    BLAST
    Propeptidei267 – 373107PRO_0000285125Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi68 ↔ 142PROSITE-ProRule annotation
    Disulfide bondi71 ↔ 136PROSITE-ProRule annotation
    Glycosylationi73 – 731N-linked (GlcNAc...)PROSITE-ProRule annotation
    Disulfide bondi99 ↔ 111PROSITE-ProRule annotation
    Disulfide bondi195 ↔ 264PROSITE-ProRule annotation
    Disulfide bondi198 ↔ 258PROSITE-ProRule annotation
    Disulfide bondi222 ↔ 233PROSITE-ProRule annotation
    Disulfide bondi235 – 235InterchainPROSITE-ProRule annotation
    Disulfide bondi291 ↔ 358PROSITE-ProRule annotation
    Disulfide bondi294 ↔ 352PROSITE-ProRule annotation
    Glycosylationi303 – 3031N-linked (GlcNAc...)PROSITE-ProRule annotation
    Disulfide bondi317 ↔ 327PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000042549.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2IL7model-A188-266[»]
    2IP3model-A188-266[»]
    ProteinModelPortaliP15781.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 6441Saposin A-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini64 – 14683Saposin B-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini191 – 26878Saposin B-type 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini287 – 36276Saposin B-type 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 saposin A-type domain.PROSITE-ProRule annotation
    Contains 3 saposin B-type domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG286450.
    GeneTreeiENSGT00530000063434.
    HOGENOMiHOG000115745.
    HOVERGENiHBG006905.
    InParanoidiP15781.
    OMAiCWLCRTL.
    OrthoDBiEOG7F24TB.
    TreeFamiTF316942.

    Family and domain databases

    Gene3Di1.10.225.10. 3 hits.
    InterProiIPR003119. SapA.
    IPR007856. SapB_1.
    IPR008138. SapB_2.
    IPR008373. Saposin.
    IPR011001. Saposin-like.
    IPR008139. SaposinB.
    [Graphical view]
    PfamiPF02199. SapA. 1 hit.
    PF05184. SapB_1. 1 hit.
    PF03489. SapB_2. 3 hits.
    [Graphical view]
    PRINTSiPR01797. SAPOSIN.
    SMARTiSM00162. SAPA. 1 hit.
    SM00741. SapB. 3 hits.
    [Graphical view]
    SUPFAMiSSF47862. SSF47862. 2 hits.
    PROSITEiPS51110. SAP_A. 1 hit.
    PS50015. SAP_B. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15781-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKSHLLPWL LLLPILCGPG TAAAITYSLA CAQGPEFWCQ SLEQALQCRA    50
    LGHCLQEVWG HVEADDLCQE CENISRLLTK MAKEAIFQDS VRKFLEQECD 100
    VLPLKLLAPL CRHLLDTYFP LIIEHFQSHM NPKFICQHVG LCKPRHPEPG 150
    KGPEPWGPLL DKLALPLLPG VPQAKPGPQT QDLSEQLFPI PIPYCWLCRT 200
    LIKRIQAVIP KGVLAMTVAQ VCHVVPLLVG GICQCLVERY SVILLDTLLG 250
    RMLPQLVCGL VLRCSSEDSA GPALPALGSV PGEWLPQDSD CQLCMFVTTQ 300
    AGNSSEQATP QAMRQACLGT WLDRQKCERF VEENAPRLQT LVSSGWDAHM 350
    ACQALGTCAA PFSPLQCVHS PHF 373
    Length:373
    Mass (Da):41,060
    Last modified:May 1, 2007 - v3
    Checksum:iA41A31AE5A5C0F82
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti198 – 1981C → L AA sequence (PubMed:3689402)Curated
    Sequence conflicti204 – 2041R → K AA sequence (PubMed:3689402)Curated
    Sequence conflicti233 – 2397CQCLVER → IQQLVIE AA sequence (PubMed:3689402)Curated
    Sequence conflicti245 – 2451L → LT AA sequence (PubMed:3689402)Curated
    Sequence conflicti252 – 2521Missing AA sequence (PubMed:3689402)Curated
    Sequence conflicti255 – 2551Q → N AA sequence (PubMed:3689402)Curated
    Sequence conflicti261 – 2611V → R AA sequence (PubMed:3689402)Curated
    Sequence conflicti266 – 2661S → G AA sequence (PubMed:3689402)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC118395 mRNA. Translation: AAI18396.1.
    PIRiA29667.
    RefSeqiNP_001068779.1. NM_001075311.2.
    XP_005212817.1. XM_005212760.1.
    UniGeneiBt.42696.

    Genome annotation databases

    EnsembliENSBTAT00000045134; ENSBTAP00000042549; ENSBTAG00000021230.
    GeneIDi507398.
    KEGGibta:507398.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC118395 mRNA. Translation: AAI18396.1 .
    PIRi A29667.
    RefSeqi NP_001068779.1. NM_001075311.2.
    XP_005212817.1. XM_005212760.1.
    UniGenei Bt.42696.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2IL7 model - A 188-266 [» ]
    2IP3 model - A 188-266 [» ]
    ProteinModelPortali P15781.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000042549.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000045134 ; ENSBTAP00000042549 ; ENSBTAG00000021230 .
    GeneIDi 507398.
    KEGGi bta:507398.

    Organism-specific databases

    CTDi 6439.

    Phylogenomic databases

    eggNOGi NOG286450.
    GeneTreei ENSGT00530000063434.
    HOGENOMi HOG000115745.
    HOVERGENi HBG006905.
    InParanoidi P15781.
    OMAi CWLCRTL.
    OrthoDBi EOG7F24TB.
    TreeFami TF316942.

    Miscellaneous databases

    NextBioi 20868039.

    Family and domain databases

    Gene3Di 1.10.225.10. 3 hits.
    InterProi IPR003119. SapA.
    IPR007856. SapB_1.
    IPR008138. SapB_2.
    IPR008373. Saposin.
    IPR011001. Saposin-like.
    IPR008139. SaposinB.
    [Graphical view ]
    Pfami PF02199. SapA. 1 hit.
    PF05184. SapB_1. 1 hit.
    PF03489. SapB_2. 3 hits.
    [Graphical view ]
    PRINTSi PR01797. SAPOSIN.
    SMARTi SM00162. SAPA. 1 hit.
    SM00741. SapB. 3 hits.
    [Graphical view ]
    SUPFAMi SSF47862. SSF47862. 2 hits.
    PROSITEi PS51110. SAP_A. 1 hit.
    PS50015. SAP_B. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal lung.
    2. "Protein sequence analysis studies on the low molecular weight hydrophobic proteins associated with bovine pulmonary surfactant."
      Olafson R.W., Rink U., Kielland S., Yu S.-H., Chung J., Harding P.G.R., Possmayer F.
      Biochem. Biophys. Res. Commun. 148:1406-1411(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 188-266.
    3. "Characterization of bovine surfactant proteins B and C by electrospray ionization mass spectrometry."
      Liu S., Zhao L., Manzanares D., Doherty-Kirby A., Zhang C., Possmayer F., Lajoie G.A.
      Rapid Commun. Mass Spectrom. 22:197-203(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 188-266, IDENTIFICATION BY MASS SPECTROMETRY.
    4. "Characterization of the small hydrophobic proteins associated with pulmonary surfactant."
      Yu S.-H., Chung W., Olafson R.W., Harding P.G.R., Possmayer F.
      Biochim. Biophys. Acta 921:437-448(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 188-197.

    Entry informationi

    Entry nameiPSPB_BOVIN
    AccessioniPrimary (citable) accession number: P15781
    Secondary accession number(s): Q148E8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3