Pulmonary surfactant-associated protein B precursor

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P15781 (PSPB_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Pulmonary surfactant-associated protein B
Short name=SP-B

Alternative name(s):
6 kDa protein
Pulmonary surfactant-associated proteolipid SPL(Phe)

Gene names

Name:	SFTPB
Synonyms:	SFTP3

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Subunit structure	Homodimer; disulfide-linked.
Subcellular location	Secreted › extracellular space › surface film.
Miscellaneous	Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).
Sequence similarities	Contains 1 saposin A-type domain. Contains 3 saposin B-type domains.

Ontologies

Keywords
Biological process	Gaseous exchange
Cellular component	Secreted Surface film
Domain	Repeat Signal
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	respiratory gaseous exchange Inferred from electronic annotation. Source: UniProtKB-KW sphingolipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

Potential

Propeptide

23 – 187

165

PRO_0000285124

Chain

188 – 266

Pulmonary surfactant-associated protein B

PRO_0000175240

Propeptide

267 – 373

107

PRO_0000285125

Regions

Domain

24 – 64

Saposin A-type

Domain

64 – 146

Saposin B-type 1

Domain

191 – 268

Saposin B-type 2

Domain

287 – 362

Saposin B-type 3

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

303

N-linked (GlcNAc...) Potential

Disulfide bond

68 ↔ 142

By similarity

Disulfide bond

71 ↔ 136

By similarity

Disulfide bond

99 ↔ 111

By similarity

Disulfide bond

195 ↔ 264

By similarity

Disulfide bond

198 ↔ 258

By similarity

Disulfide bond

222 ↔ 233

By similarity

Disulfide bond

235

Interchain By similarity

Disulfide bond

291 ↔ 358

By similarity

Disulfide bond

294 ↔ 352

By similarity

Disulfide bond

317 ↔ 327

By similarity

Experimental info

Sequence conflict

198

C → L AA sequence Ref.2

Sequence conflict

204

R → K AA sequence Ref.2

Sequence conflict

233 – 239

CQCLVER → IQQLVIE AA sequence Ref.2

Sequence conflict

245

L → LT AA sequence Ref.2

Sequence conflict

252

Missing AA sequence Ref.2

Sequence conflict

255

Q → N AA sequence Ref.2

Sequence conflict

261

V → R AA sequence Ref.2

Sequence conflict

266

S → G AA sequence Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P15781 [UniParc].

Last modified May 1, 2007. Version 3.
Checksum: A41A31AE5A5C0F82
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FASTA

373

41,060

        10         20         30         40         50         60 
MAKSHLLPWL LLLPILCGPG TAAAITYSLA CAQGPEFWCQ SLEQALQCRA LGHCLQEVWG 

        70         80         90        100        110        120 
HVEADDLCQE CENISRLLTK MAKEAIFQDS VRKFLEQECD VLPLKLLAPL CRHLLDTYFP 

       130        140        150        160        170        180 
LIIEHFQSHM NPKFICQHVG LCKPRHPEPG KGPEPWGPLL DKLALPLLPG VPQAKPGPQT 

       190        200        210        220        230        240 
QDLSEQLFPI PIPYCWLCRT LIKRIQAVIP KGVLAMTVAQ VCHVVPLLVG GICQCLVERY 

       250        260        270        280        290        300 
SVILLDTLLG RMLPQLVCGL VLRCSSEDSA GPALPALGSV PGEWLPQDSD CQLCMFVTTQ 

       310        320        330        340        350        360 
AGNSSEQATP QAMRQACLGT WLDRQKCERF VEENAPRLQT LVSSGWDAHM ACQALGTCAA 

       370 
PFSPLQCVHS PHF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal lung.
[2]	"Protein sequence analysis studies on the low molecular weight hydrophobic proteins associated with bovine pulmonary surfactant." Olafson R.W., Rink U., Kielland S., Yu S.-H., Chung J., Harding P.G.R., Possmayer F. Biochem. Biophys. Res. Commun. 148:1406-1411(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 188-266.
[3]	"Characterization of bovine surfactant proteins B and C by electrospray ionization mass spectrometry." Liu S., Zhao L., Manzanares D., Doherty-Kirby A., Zhang C., Possmayer F., Lajoie G.A. Rapid Commun. Mass Spectrom. 22:197-203(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 188-266, MASS SPECTROMETRY.
[4]	"Characterization of the small hydrophobic proteins associated with pulmonary surfactant." Yu S.-H., Chung W., Olafson R.W., Harding P.G.R., Possmayer F. Biochim. Biophys. Acta 921:437-448(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 188-197.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BC118395 mRNA. Translation: AAI18396.1.

IPI

IPI00709322.

PIR

A29667.

RefSeq

NP_001068779.1. NM_001075311.2.

UniGene

Bt.42696.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IL7	model	-	A	188-266	[»]
2IP3	model	-	A	188-266	[»]

ProteinModelPortal

P15781.

ModBase

Search...

Protein-protein interaction databases

STRING

9913.ENSBTAP00000042549.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSBTAT00000045134; ENSBTAP00000042549; ENSBTAG00000021230.

GeneID

507398.

KEGG

bta:507398.

Organism-specific databases

CTD

6439.

Phylogenomic databases

eggNOG

NOG286450.

GeneTree

ENSGT00530000063434.

HOGENOM

HOG000115745.

HOVERGEN

HBG006905.

InParanoid

P15781.

OMA

CWLCRTL.

OrthoDB

EOG434W6C.

Family and domain databases

Gene3D

1.10.225.10. 3 hits.

InterPro

IPR003119. SapA.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]

Pfam

PF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 3 hits.
[Graphical view]

PRINTS

PR01797. SAPOSIN.

SMART

SM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]

SUPFAM

SSF47862. Saposin_like. 3 hits.

PROSITE

PS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

20868039.

Entry information

Entry name

PSPB_BOVIN

Accession

Primary (citable) accession number: P15781
Secondary accession number(s): Q148E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	May 1, 2007
Last modified:	April 3, 2013
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents