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P15776

- HEMA_CVBM

UniProt

P15776 - HEMA_CVBM

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Protein

Hemagglutinin-esterase

Gene
HE, 2b
Organism
Bovine coronavirus (strain Mebus) (BCoV) (BCV)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401Nucleophile By similarity
Active sitei228 – 2281Charge relay system By similarity
Active sitei329 – 3291Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiBovine coronavirus (strain Mebus) (BCoV) (BCV)
Taxonomic identifieri11132 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000007554: Genome

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 392374Virion surface Reviewed predictionAdd
BLAST
Transmembranei393 – 41321Helical; Reviewed predictionAdd
BLAST
Topological domaini414 – 42411Intravirion Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18181 PublicationAdd
BLAST
Chaini19 – 424406Hemagglutinin-esterasePRO_0000037138Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 651 Publication
Glycosylationi54 – 541N-linked (GlcNAc...); by host1 Publication
Glycosylationi89 – 891N-linked (GlcNAc...); by host1 Publication
Disulfide bondi113 ↔ 1621 Publication
Glycosylationi153 – 1531N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi197 ↔ 2761 Publication
Disulfide bondi205 ↔ 2491 Publication
Glycosylationi236 – 2361N-linked (GlcNAc...); by host1 Publication
Glycosylationi301 – 3011N-linked (GlcNAc...); by host1 Publication
Disulfide bondi307 ↔ 3121 Publication
Glycosylationi316 – 3161N-linked (GlcNAc...); by host1 Publication
Disulfide bondi347 ↔ 3711 Publication
Glycosylationi358 – 3581N-linked (GlcNAc...); by host1 Publication

Post-translational modificationi

N-glycosylated in the RER.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.1 Publication

Structurei

Secondary structure

1
424
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi23 – 253
Beta strandi29 – 324
Beta strandi34 – 396
Helixi40 – 423
Helixi44 – 496
Beta strandi50 – 523
Helixi55 – 573
Helixi62 – 643
Beta strandi70 – 734
Helixi78 – 836
Beta strandi84 – 863
Beta strandi95 – 1006
Helixi107 – 1093
Helixi119 – 13719
Beta strandi140 – 1478
Beta strandi150 – 1523
Beta strandi162 – 1643
Beta strandi167 – 1693
Beta strandi171 – 1755
Beta strandi182 – 1843
Beta strandi188 – 20720
Beta strandi211 – 2133
Beta strandi216 – 2183
Beta strandi221 – 2266
Turni227 – 2293
Beta strandi232 – 2365
Beta strandi242 – 2443
Beta strandi247 – 27024
Beta strandi273 – 2808
Beta strandi286 – 2894
Helixi302 – 3065
Turni309 – 3113
Beta strandi312 – 3154
Beta strandi323 – 3264
Beta strandi329 – 3313
Helixi332 – 3387
Helixi339 – 3424
Beta strandi346 – 3494
Beta strandi352 – 3554
Beta strandi357 – 3593
Beta strandi368 – 3703

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CL4X-ray2.10A19-388[»]
3CL5X-ray1.80A19-388[»]
ProteinModelPortaliP15776.
SMRiP15776. Positions 19-376.

Miscellaneous databases

EvolutionaryTraceiP15776.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 127121Esterase domain first part By similarityAdd
BLAST
Regioni128 – 266139Receptor binding By similarityAdd
BLAST
Regioni267 – 379113Esterase domain second part By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15776-1 [UniParc]FASTAAdd to Basket

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MFLLLRFVLV SCIIGSLGFD NPPTNVVSHL NGDWFLFGDS RSDCNHVVNT    50
NPRNYSYMDL NPALCDSGKI SSKAGNSIFR SFHFTDFYNY TGEGQQIIFY 100
EGVNFTPYHA FKCTTSGSND IWMQNKGLFY TQVYKNMAVY RSLTFVNVPY 150
VYNGSAQSTA LCKSGSLVLN NPAYIAREAN FGDYYYKVEA DFYLSGCDEY 200
IVPLCIFNGK FLSNTKYYDD SQYYFNKDTG VIYGLNSTET ITTGFDFNCH 250
YLVLPSGNYL AISNELLLTV PTKAICLNKR KDFTPVQVVD SRWNNARQSD 300
NMTAVACQPP YCYFRNSTTN YVGVYDINHG DAGFTSILSG LLYDSPCFSQ 350
QGVFRYDNVS SVWPLYSYGR CPTAADINTP DVPICVYDPL PLILLGILLG 400
VAVIIIVVLL LYFMVDNGTR LHDA 424
Length:424
Mass (Da):47,695
Last modified:April 1, 1990 - v1
Checksum:iFDEF70542F91F305
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00735 Genomic RNA. Translation: AAA66393.1.
S50936 Genomic RNA. Translation: AAB19562.1.
PIRiA34666. HMIHBC.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00735 Genomic RNA. Translation: AAA66393.1 .
S50936 Genomic RNA. Translation: AAB19562.1 .
PIRi A34666. HMIHBC.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3CL4 X-ray 2.10 A 19-388 [» ]
3CL5 X-ray 1.80 A 19-388 [» ]
ProteinModelPortali P15776.
SMRi P15776. Positions 19-376.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P15776.

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein."
    Kienzle T.E., Abraham S., Hogue B.G., Brian D.A.
    J. Virol. 64:1834-1838(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], SUBCELLULAR LOCATION.
  2. "Structure and expression of the bovine coronavirus hemagglutinin protein."
    Kienzle T.E., Abraham S., Hogue B.G., Brian D.A.
    Adv. Exp. Med. Biol. 276:95-102(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Synthesis and processing of the bovine enteric coronavirus haemagglutinin protein."
    Hogue B.G., Kienzle T.E., Brian D.A.
    J. Gen. Virol. 70:345-352(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-27.
  4. "Nidovirus sialate-O-acetylesterases: evolution and substrate specificity of coronaviral and toroviral receptor-destroying enzymes."
    Smits S.L., Gerwig G.J., van Vliet A.L., Lissenberg A., Briza P., Kamerling J.P., Vlasak R., de Groot R.J.
    J. Biol. Chem. 280:6933-6941(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Structure of coronavirus hemagglutinin-esterase offers insight into corona and influenza virus evolution."
    Zeng Q., Langereis M.A., van Vliet A.L., Huizinga E.G., de Groot R.J.
    Proc. Natl. Acad. Sci. U.S.A. 105:9065-9069(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 19-388, GLYCOSYLATION AT ASN-54; ASN-89; ASN-236; ASN-301; ASN-316 AND ASN-358, DISULFIDE BONDS.

Entry informationi

Entry nameiHEMA_CVBM
AccessioniPrimary (citable) accession number: P15776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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