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Protein

Nucleolin

Gene

NCL

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolin
Alternative name(s):
Protein C23
Gene namesi
Name:NCL
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 694694NucleolinPRO_0000081695Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 1161PhosphoserineBy similarity
Modified residuei136 – 1361PhosphoserineBy similarity
Modified residuei171 – 1711PhosphoserineBy similarity

Post-translational modificationi

Highly phosphorylated during mitosis.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiP15771.
PRIDEiP15771.

Interactioni

Protein-protein interaction databases

BioGridi676461. 1 interaction.
STRINGi9031.ENSGALP00000012472.

Structurei

3D structure databases

ProteinModelPortaliP15771.
SMRiP15771. Positions 459-535, 549-629.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati55 – 6171
Repeati62 – 6872
Repeati69 – 7573
Repeati76 – 8274
Repeati84 – 9075
Domaini281 – 35777RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini371 – 44575RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini461 – 53575RRM 3PROSITE-ProRule annotationAdd
BLAST
Domaini553 – 62876RRM 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 90365 X 7 AA tandem repeats of X-T-P-X-K-K-XAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi124 – 14118Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi170 – 19223Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi217 – 24731Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi630 – 68253Arg/Gly/Phe-richAdd
BLAST

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IRRZ. Eukaryota.
ENOG411015D. LUCA.
HOGENOMiHOG000113885.
HOVERGENiHBG002295.
InParanoidiP15771.
KOiK11294.
PhylomeDBiP15771.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.70.330. 4 hits.
InterProiIPR011989. ARM-like.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
SM00361. RRM_1. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLAKTPKN QMKQKKMAPP PKKVEESEEE ESSDLEESSG EEVMVPPKKQ
60 70 80 90 100
QKAAVTPAKK AATPAKKAAT PAKKAVTPAK KAVATPAKKA VAPSPKKAAV
110 120 130 140 150
VGKGAKNGKN AKKEESEEED EDDEDDEEDE DEEEESDEEE EPAVPVKPAA
160 170 180 190 200
KKSAAAVPAK KPAVVPAKQE SEEEEEEDDE EEDEEDDESE DEAMDTTPAP
210 220 230 240 250
VKKPTPAKAT PAKAKAESED EEDEEDEDED EEDEDDEEED EEESEDEKPV
260 270 280 290 300
KEAPGKRKKE MANKSAPEAK KKKTETPASA FSLFVKNLTP TKDYEELRTA
310 320 330 340 350
IKEFFGKKNL QVSEVRIGSS KRFGYVDFLS AEDMDKALQL NGKKLMGLEI
360 370 380 390 400
KLEKAKSKES LKENKKERDA RTLFVKNLPY RVTEDEMKNV FENALEVRLV
410 420 430 440 450
LNKEGSSKGM AYIEFKTEAE AEKALEEKQG TEVDGRAMVI DYTGEKSQQE
460 470 480 490 500
SQKGGGERES KTLIVNNLSY AASEETLQEL FKKATSIKMP QNNQGRPKGY
510 520 530 540 550
AFVEFPTAED AKEALNSCNN TEIEGRAIRL EFSSPSWQKG NMNARGGFNQ
560 570 580 590 600
QSKTLFVRGL SEDTTEETLR ESFEGSISAR IVTDRDTGSS KGFGFVDFSS
610 620 630 640 650
PEDAKAAKEA MEDGEIDGNK VTLDFAKPKG EFQRGGGFGG GFGGRGGRGG
660 670 680 690
RGGGRGGFGG RGGGRGFGGR GGGFRGGRGG GGDHKPQGKK IKFE
Length:694
Mass (Da):75,640
Last modified:April 1, 1990 - v1
Checksum:i7996C504BE9459A1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti419 – 4191A → R in AAA48983 (PubMed:2914325).Curated
Sequence conflicti520 – 5201N → T in AAA48983 (PubMed:2914325).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17199 mRNA. Translation: CAA35060.1.
M21791 mRNA. Translation: AAA48983.1.
PIRiS08414. DNCHNL.
RefSeqiNP_990596.1. NM_205265.1.
UniGeneiGga.3663.

Genome annotation databases

GeneIDi396201.
KEGGigga:396201.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17199 mRNA. Translation: CAA35060.1.
M21791 mRNA. Translation: AAA48983.1.
PIRiS08414. DNCHNL.
RefSeqiNP_990596.1. NM_205265.1.
UniGeneiGga.3663.

3D structure databases

ProteinModelPortaliP15771.
SMRiP15771. Positions 459-535, 549-629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676461. 1 interaction.
STRINGi9031.ENSGALP00000012472.

Proteomic databases

PaxDbiP15771.
PRIDEiP15771.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396201.
KEGGigga:396201.

Organism-specific databases

CTDi4691.

Phylogenomic databases

eggNOGiENOG410IRRZ. Eukaryota.
ENOG411015D. LUCA.
HOGENOMiHOG000113885.
HOVERGENiHBG002295.
InParanoidiP15771.
KOiK11294.
PhylomeDBiP15771.

Miscellaneous databases

PROiP15771.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.70.330. 4 hits.
InterProiIPR011989. ARM-like.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
SM00361. RRM_1. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA sequences of chicken nucleolin/C23 and NO38/B23, two major nucleolar proteins."
    Maridor G., Nigg E.A.
    Nucleic Acids Res. 18:1286-1286(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure and developmental expression of chicken nucleolin and NO38: coordinate expression of two abundant non-ribosomal nucleolar proteins."
    Maridor G., Krek W., Nigg E.A.
    Biochim. Biophys. Acta 1049:126-133(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.
  3. "Major nucleolar proteins shuttle between nucleus and cytoplasm."
    Borer R.A., Lehner C.F., Eppenberger H.M., Nigg E.A.
    Cell 56:379-390(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 407-694.
  4. "Identification of major nucleolar proteins as candidate mitotic substrates of cdc2 kinase."
    Peter M., Nakagawa J., Doree M., Labbe J.C., Nigg E.A.
    Cell 60:791-801(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.

Entry informationi

Entry nameiNUCL_CHICK
AccessioniPrimary (citable) accession number: P15771
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.