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Protein

Nucleolin

Gene

NCL

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolin
Alternative name(s):
Protein C23
Gene namesi
Name:NCL
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816951 – 694NucleolinAdd BLAST694

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116PhosphoserineBy similarity1
Modified residuei136PhosphoserineBy similarity1
Modified residuei171PhosphoserineBy similarity1

Post-translational modificationi

Highly phosphorylated during mitosis.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiP15771.
PRIDEiP15771.

Interactioni

Protein-protein interaction databases

BioGridi676461. 1 interactor.
STRINGi9031.ENSGALP00000012472.

Structurei

3D structure databases

ProteinModelPortaliP15771.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati55 – 6117
Repeati62 – 6827
Repeati69 – 7537
Repeati76 – 8247
Repeati84 – 9057
Domaini281 – 357RRM 1PROSITE-ProRule annotationAdd BLAST77
Domaini371 – 445RRM 2PROSITE-ProRule annotationAdd BLAST75
Domaini461 – 535RRM 3PROSITE-ProRule annotationAdd BLAST75
Domaini553 – 628RRM 4PROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 905 X 7 AA tandem repeats of X-T-P-X-K-K-XAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi124 – 141Asp/Glu-rich (acidic)Add BLAST18
Compositional biasi170 – 192Asp/Glu-rich (acidic)Add BLAST23
Compositional biasi217 – 247Asp/Glu-rich (acidic)Add BLAST31
Compositional biasi630 – 682Arg/Gly/Phe-richAdd BLAST53

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IRRZ. Eukaryota.
ENOG411015D. LUCA.
HOGENOMiHOG000113885.
HOVERGENiHBG002295.
InParanoidiP15771.
KOiK11294.
PhylomeDBiP15771.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.70.330. 4 hits.
InterProiIPR011989. ARM-like.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
SM00361. RRM_1. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLAKTPKN QMKQKKMAPP PKKVEESEEE ESSDLEESSG EEVMVPPKKQ
60 70 80 90 100
QKAAVTPAKK AATPAKKAAT PAKKAVTPAK KAVATPAKKA VAPSPKKAAV
110 120 130 140 150
VGKGAKNGKN AKKEESEEED EDDEDDEEDE DEEEESDEEE EPAVPVKPAA
160 170 180 190 200
KKSAAAVPAK KPAVVPAKQE SEEEEEEDDE EEDEEDDESE DEAMDTTPAP
210 220 230 240 250
VKKPTPAKAT PAKAKAESED EEDEEDEDED EEDEDDEEED EEESEDEKPV
260 270 280 290 300
KEAPGKRKKE MANKSAPEAK KKKTETPASA FSLFVKNLTP TKDYEELRTA
310 320 330 340 350
IKEFFGKKNL QVSEVRIGSS KRFGYVDFLS AEDMDKALQL NGKKLMGLEI
360 370 380 390 400
KLEKAKSKES LKENKKERDA RTLFVKNLPY RVTEDEMKNV FENALEVRLV
410 420 430 440 450
LNKEGSSKGM AYIEFKTEAE AEKALEEKQG TEVDGRAMVI DYTGEKSQQE
460 470 480 490 500
SQKGGGERES KTLIVNNLSY AASEETLQEL FKKATSIKMP QNNQGRPKGY
510 520 530 540 550
AFVEFPTAED AKEALNSCNN TEIEGRAIRL EFSSPSWQKG NMNARGGFNQ
560 570 580 590 600
QSKTLFVRGL SEDTTEETLR ESFEGSISAR IVTDRDTGSS KGFGFVDFSS
610 620 630 640 650
PEDAKAAKEA MEDGEIDGNK VTLDFAKPKG EFQRGGGFGG GFGGRGGRGG
660 670 680 690
RGGGRGGFGG RGGGRGFGGR GGGFRGGRGG GGDHKPQGKK IKFE
Length:694
Mass (Da):75,640
Last modified:April 1, 1990 - v1
Checksum:i7996C504BE9459A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti419A → R in AAA48983 (PubMed:2914325).Curated1
Sequence conflicti520N → T in AAA48983 (PubMed:2914325).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17199 mRNA. Translation: CAA35060.1.
M21791 mRNA. Translation: AAA48983.1.
PIRiS08414. DNCHNL.
RefSeqiNP_990596.1. NM_205265.1.
UniGeneiGga.3663.

Genome annotation databases

GeneIDi396201.
KEGGigga:396201.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17199 mRNA. Translation: CAA35060.1.
M21791 mRNA. Translation: AAA48983.1.
PIRiS08414. DNCHNL.
RefSeqiNP_990596.1. NM_205265.1.
UniGeneiGga.3663.

3D structure databases

ProteinModelPortaliP15771.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676461. 1 interactor.
STRINGi9031.ENSGALP00000012472.

Proteomic databases

PaxDbiP15771.
PRIDEiP15771.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396201.
KEGGigga:396201.

Organism-specific databases

CTDi4691.

Phylogenomic databases

eggNOGiENOG410IRRZ. Eukaryota.
ENOG411015D. LUCA.
HOGENOMiHOG000113885.
HOVERGENiHBG002295.
InParanoidiP15771.
KOiK11294.
PhylomeDBiP15771.

Miscellaneous databases

PROiP15771.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.70.330. 4 hits.
InterProiIPR011989. ARM-like.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
SM00361. RRM_1. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUCL_CHICK
AccessioniPrimary (citable) accession number: P15771
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 5, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.