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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA). It displays no activity in the presence of NAD.UniRule annotation2 Publications

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation1 Publication

Kineticsi

Kcat is 14.2 min(-1) for dehydrogenase activity with NADP or shikimate.1 Publication

  1. KM=56 µM for NADP1 Publication
  2. KM=65 µM for shikimate1 Publication

    Pathwayi: chorismate biosynthesis

    This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
    Proteins known to be involved in the 7 steps of the subpathway in this organism are:
    1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG)
    2. 3-dehydroquinate synthase (aroB)
    3. 3-dehydroquinate dehydratase (aroD)
    4. Quinate/shikimate dehydrogenase (ydiB), Shikimate dehydrogenase (NADP(+)) (aroE)
    5. Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
    6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
    7. Chorismate synthase (aroC)
    This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611ShikimateUniRule annotation
    Active sitei65 – 651Proton acceptorUniRule annotation
    Binding sitei77 – 771NADPUniRule annotation
    Binding sitei86 – 861ShikimateUniRule annotation
    Binding sitei102 – 1021ShikimateUniRule annotation
    Binding sitei213 – 2131NADP; via carbonyl oxygenUniRule annotation1 Publication
    Binding sitei215 – 2151ShikimateUniRule annotation
    Binding sitei237 – 2371NADP; via carbonyl oxygenUniRule annotation1 Publication
    Binding sitei244 – 2441ShikimateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi126 – 1305NADPUniRule annotation1 Publication
    Nucleotide bindingi149 – 1546NADPUniRule annotation1 Publication

    GO - Molecular functioni

    • NADP binding Source: EcoCyc
    • nucleotide binding Source: EcoCyc
    • shikimate 3-dehydrogenase (NADP+) activity Source: EcoCyc

    GO - Biological processi

    • aromatic amino acid family biosynthetic process Source: UniProtKB-HAMAP
    • chorismate biosynthetic process Source: EcoCyc
    • shikimate metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:AROE-MONOMER.
    ECOL316407:JW3242-MONOMER.
    MetaCyc:AROE-MONOMER.
    SABIO-RKP15770.
    UniPathwayiUPA00053; UER00087.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Shikimate dehydrogenase (NADP(+))1 Publication (EC:1.1.1.251 Publication)
    Short name:
    SD1 Publication
    Short name:
    SDHUniRule annotation
    Gene namesi
    Name:aroE1 PublicationUniRule annotation
    Ordered Locus Names:b3281, JW3242
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10077. aroE.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 272272Shikimate dehydrogenase (NADP(+))PRO_0000136002Add
    BLAST

    Proteomic databases

    PaxDbiP15770.

    Interactioni

    Subunit structurei

    Monomer or homodimer.UniRule annotation2 Publications

    Protein-protein interaction databases

    BioGridi4261870. 136 interactions.
    DIPiDIP-9153N.
    IntActiP15770. 6 interactions.
    MINTiMINT-1246863.
    STRINGi511145.b3281.

    Structurei

    Secondary structure

    1
    272
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 108Combined sources
    Helixi16 – 2712Combined sources
    Beta strandi33 – 375Combined sources
    Helixi43 – 5210Combined sources
    Beta strandi57 – 604Combined sources
    Helixi65 – 717Combined sources
    Beta strandi73 – 753Combined sources
    Helixi77 – 826Combined sources
    Beta strandi86 – 905Combined sources
    Beta strandi96 – 994Combined sources
    Helixi102 – 11211Combined sources
    Beta strandi121 – 1255Combined sources
    Helixi129 – 14012Combined sources
    Beta strandi144 – 1485Combined sources
    Helixi152 – 16110Combined sources
    Helixi163 – 1653Combined sources
    Beta strandi166 – 1705Combined sources
    Helixi174 – 1763Combined sources
    Beta strandi182 – 1865Combined sources
    Helixi191 – 1933Combined sources
    Helixi201 – 2033Combined sources
    Beta strandi209 – 2135Combined sources
    Helixi221 – 2288Combined sources
    Beta strandi233 – 2353Combined sources
    Helixi238 – 25316Combined sources
    Helixi259 – 27012Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NYTX-ray1.50A/B/C/D1-271[»]
    ProteinModelPortaliP15770.
    SMRiP15770. Positions 1-271.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP15770.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni14 – 163Shikimate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the shikimate dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105E2X. Bacteria.
    COG0169. LUCA.
    HOGENOMiHOG000237876.
    InParanoidiP15770.
    KOiK00014.
    OMAiFGNPIKH.
    PhylomeDBiP15770.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR011342. Shikimate_DH.
    IPR013708. Shikimate_DH-bd_N.
    IPR022893. Shikimate_DH_fam.
    IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
    [Graphical view]
    PfamiPF01488. Shikimate_DH. 1 hit.
    PF08501. Shikimate_dh_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.
    TIGRFAMsiTIGR00507. aroE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P15770-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    METYAVFGNP IAHSKSPFIH QQFAQQLNIE HPYGRVLAPI NDFINTLNAF
    60 70 80 90 100
    FSAGGKGANV TVPFKEEAFA RADELTERAA LAGAVNTLMR LEDGRLLGDN
    110 120 130 140 150
    TDGVGLLSDL ERLSFIRPGL RILLIGAGGA SRGVLLPLLS LDCAVTITNR
    160 170 180 190 200
    TVSRAEELAK LFAHTGSIQA LSMDELEGHE FDLIINATSS GISGDIPAIP
    210 220 230 240 250
    SSLIHPGIYC YDMFYQKGKT PFLAWCEQRG SKRNADGLGM LVAQAAHAFL
    260 270
    LWHGVLPDVE PVIKQLQEEL SA
    Length:272
    Mass (Da):29,414
    Last modified:April 1, 1990 - v1
    Checksum:i62D3797ECA1EC1E8
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y00710 Genomic DNA. Translation: CAA68700.1.
    U18997 Genomic DNA. Translation: AAA58078.1.
    U00096 Genomic DNA. Translation: AAC76306.1.
    AP009048 Genomic DNA. Translation: BAE78010.1.
    PIRiS00252.
    RefSeqiNP_417740.1. NC_000913.3.
    WP_000451243.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76306; AAC76306; b3281.
    BAE78010; BAE78010; BAE78010.
    GeneIDi947776.
    KEGGiecj:JW3242.
    eco:b3281.
    PATRICi32121996. VBIEscCol129921_3375.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y00710 Genomic DNA. Translation: CAA68700.1.
    U18997 Genomic DNA. Translation: AAA58078.1.
    U00096 Genomic DNA. Translation: AAC76306.1.
    AP009048 Genomic DNA. Translation: BAE78010.1.
    PIRiS00252.
    RefSeqiNP_417740.1. NC_000913.3.
    WP_000451243.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NYTX-ray1.50A/B/C/D1-271[»]
    ProteinModelPortaliP15770.
    SMRiP15770. Positions 1-271.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261870. 136 interactions.
    DIPiDIP-9153N.
    IntActiP15770. 6 interactions.
    MINTiMINT-1246863.
    STRINGi511145.b3281.

    Proteomic databases

    PaxDbiP15770.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76306; AAC76306; b3281.
    BAE78010; BAE78010; BAE78010.
    GeneIDi947776.
    KEGGiecj:JW3242.
    eco:b3281.
    PATRICi32121996. VBIEscCol129921_3375.

    Organism-specific databases

    EchoBASEiEB0075.
    EcoGeneiEG10077. aroE.

    Phylogenomic databases

    eggNOGiENOG4105E2X. Bacteria.
    COG0169. LUCA.
    HOGENOMiHOG000237876.
    InParanoidiP15770.
    KOiK00014.
    OMAiFGNPIKH.
    PhylomeDBiP15770.

    Enzyme and pathway databases

    UniPathwayiUPA00053; UER00087.
    BioCyciEcoCyc:AROE-MONOMER.
    ECOL316407:JW3242-MONOMER.
    MetaCyc:AROE-MONOMER.
    SABIO-RKP15770.

    Miscellaneous databases

    EvolutionaryTraceiP15770.
    PROiP15770.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR011342. Shikimate_DH.
    IPR013708. Shikimate_DH-bd_N.
    IPR022893. Shikimate_DH_fam.
    IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
    [Graphical view]
    PfamiPF01488. Shikimate_DH. 1 hit.
    PF08501. Shikimate_dh_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.
    TIGRFAMsiTIGR00507. aroE. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiAROE_ECOLI
    AccessioniPrimary (citable) accession number: P15770
    Secondary accession number(s): Q2M6U6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: September 7, 2016
    This is version 154 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.