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P15770 (AROE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Shikimate dehydrogenase
EC=1.1.1.25
Gene names
Name:aroE
Ordered Locus Names:b3281, JW3242
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222

Subunit structure

Monomer. Ref.5

Sequence similarities

Belongs to the shikimate dehydrogenase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

yedEP310641EBI-544087,EBI-544094

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 272272Shikimate dehydrogenase HAMAP MF_00222
PRO_0000136002

Regions

Nucleotide binding126 – 1305NAD HAMAP MF_00222
Nucleotide binding149 – 1546NAD HAMAP MF_00222
Nucleotide binding237 – 2415NAD HAMAP MF_00222

Sites

Active site651Proton acceptor Potential
Binding site1021Substrate By similarity
Binding site1891NAD; via amide nitrogen
Binding site2131NAD; via carbonyl oxygen

Secondary structure

1 .................................................... 272
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P15770 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 62D3797ECA1EC1E8

FASTA27229,414
        10         20         30         40         50         60 
METYAVFGNP IAHSKSPFIH QQFAQQLNIE HPYGRVLAPI NDFINTLNAF FSAGGKGANV 

        70         80         90        100        110        120 
TVPFKEEAFA RADELTERAA LAGAVNTLMR LEDGRLLGDN TDGVGLLSDL ERLSFIRPGL 

       130        140        150        160        170        180 
RILLIGAGGA SRGVLLPLLS LDCAVTITNR TVSRAEELAK LFAHTGSIQA LSMDELEGHE 

       190        200        210        220        230        240 
FDLIINATSS GISGDIPAIP SSLIHPGIYC YDMFYQKGKT PFLAWCEQRG SKRNADGLGM 

       250        260        270 
LVAQAAHAFL LWHGVLPDVE PVIKQLQEEL SA

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase."
Anton I.A., Coggins J.R.
Biochem. J. 249:319-326(1988) [PubMed: 3277621] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-30.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Crystallization and preliminary X-ray analysis of shikimate dehydrogenase from Escherichia coli."
Maclean J., Campbell S.A., Pollock K., Chackrewarthy S., Coggins J.R., Lapthorn A.J.
Acta Crystallogr. D 56:512-515(2000) [PubMed: 10739937] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[5]"Structures of shikimate dehydrogenase AroE and its paralog YdiB. A common structural framework for different activities."
Michel G., Roszak A.W., Sauve V., Maclean J., Matte A., Coggins J.R., Cygler M., Lapthorn A.J.
J. Biol. Chem. 278:19463-19472(2003) [PubMed: 12637497] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) IN COMPLEX WITH NADP, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Y00710 Genomic DNA. Translation: CAA68700.1.
U18997 Genomic DNA. Translation: AAA58078.1.
U00096 Genomic DNA. Translation: AAC76306.1.
AP009048 Genomic DNA. Translation: BAE78010.1.
PIRS00252.
RefSeqNP_417740.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NYTX-ray1.50A/B/C/D1-271[»]
ProteinModelPortalP15770.
SMRP15770. Positions 1-271.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-9153N.
IntActP15770. 3 interactions.
MINTMINT-1246863.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000001413; EBESCP00000001413; EBESCG00000001177.
EBESCT00000016050; EBESCP00000015341; EBESCG00000015110.
GeneID947776.
GenomeReviewsGene locus JW3242 in contig AP009048_GR.
Gene locus b3281 in contig U00096_GR.
KEGGecj:JW3242.
eco:b3281.
PATRIC32121996. VBIEscCol129921_3375.

Organism-specific databases

EchoBASEEB0075.
EcoGeneEG10077. aroE.

Phylogenomic databases

eggNOGCOG0169.
GeneTreeEBGT00050000010797.
HOGENOMHBG553408.
OMAVVFGNPI.
PhylomeDBP15770.
ProtClustDBPRK00258.

Enzyme and pathway databases

BioCycEcoCyc:AROE-MONOMER.
MetaCyc:AROE-MONOMER.
BRENDA1.1.1.25. 2026.

Gene expression databases

GenevestigatorP15770.

Family and domain databases

HAMAPMF_00222. Shikimate_DH_AroE.
[Tree]
InterProIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_quinate_DH.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00014.
PfamPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00507. AroE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAROE_ECOLI
AccessionPrimary (citable) accession number: P15770
Secondary accession number(s): Q2M6U6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: January 25, 2012
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents