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Protein

Type 4 prepilin-like proteins leader peptide-processing enzyme

Gene

pulO

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Required for the translocation of pullulanase.

Catalytic activityi

Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Methyltransferase, Protease, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

MEROPSiA24.A10.
TCDBi3.A.15.1.1. the outer membrane protein secreting main terminal branch (mtb) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzyme
Alternative name(s):
Pullulanase secretion protein PulO
Including the following 2 domains:
Leader peptidase (EC:3.4.23.43)
Alternative name(s):
Prepilin peptidase
N-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:pulO
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei18 – 3821HelicalSequence analysisAdd
BLAST
Transmembranei95 – 11521HelicalSequence analysisAdd
BLAST
Transmembranei116 – 13621HelicalSequence analysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence analysisAdd
BLAST
Transmembranei168 – 18821HelicalSequence analysisAdd
BLAST
Transmembranei204 – 22421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228Type 4 prepilin-like proteins leader peptide-processing enzymePRO_0000192621Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.

Sequencei

Sequence statusi: Complete.

P15754-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVENIALLPE FAAQYPFLWG SFLFLSGLAF GSFFNVVIHR LPLMMEQAEG
60 70 80 90 100
INLCFPASFC PQCREPIAWR DNIPLLGFLF LKGRSRCCGQ PISPRYPLME
110 120 130 140 150
LATGALFVLA GYLMAPGVPL LGGLILLSLL LILAAIDAQT QLLPDGLTLP
160 170 180 190 200
LMWAGLLFNL SATYVPLAEA VVGAMAGYLS LWSVYWVFRL LSGKEALGYG
210 220
DFKLLAALGA WLGWQALPQT LLLASPAA
Length:228
Mass (Da):24,754
Last modified:April 1, 1990 - v1
Checksum:i38D5EE1A9E3BC7B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32613 Genomic DNA. Translation: AAA25137.1.
X52462 Genomic DNA. Translation: CAA36700.1.
PIRiS11803.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32613 Genomic DNA. Translation: AAA25137.1.
X52462 Genomic DNA. Translation: CAA36700.1.
PIRiS11803.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiA24.A10.
TCDBi3.A.15.1.1. the outer membrane protein secreting main terminal branch (mtb) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.
ProtoNetiSearch...

Entry informationi

Entry nameiLEP4_KLEPN
AccessioniPrimary (citable) accession number: P15754
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 14, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.