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P15736 (VP3_ROTS1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein VP3

Including the following 2 domains:

  1. mRNA guanylyltransferase
    EC=2.7.7.50
  2. mRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.56
OrganismRotavirus A (strain SA11-Both G3-P5B[2]-I2-R2-C5-M5-A5-N2-T5-E2-H5) (RV-A) (Simian Agent 11 (strain Both)) [Complete proteome]
Taxonomic identifier37137 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.

Catalytic activity

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

Subunit structure

Interacts with VP1 Potential. Interacts with VP2 By similarity.

Subcellular location

Virion Potential. Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.

Sequence similarities

Belongs to the rotavirus VP3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 835835Protein VP3
PRO_0000149537

Regions

Compositional bias270 – 2734Poly-Tyr

Experimental info

Sequence conflict251I → T in CAA34198. Ref.2
Sequence conflict1361G → A in CAA34198. Ref.2
Sequence conflict3881H → D in CAA34198. Ref.2
Sequence conflict6131I → V in CAA34198. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P15736 [UniParc].

Last modified August 1, 1991. Version 2.
Checksum: D67C329D6A29B680

FASTA83598,141
        10         20         30         40         50         60 
MKVLALRHSV AQVYADTQVY VHDDIKDSYE NAFLISNLTT HNILYLNYSI KTLEILNKSG 

        70         80         90        100        110        120 
IAAIALQSLE ELFTLIRCNF TYDYELDIIY LHDYSYYTNN EIRTDQHWIT KTNIEEYLLP 

       130        140        150        160        170        180 
GWKLTYVGYN GSETRGHYNF SFKCQNAATD DDLIIEYIYS EALDFQNFML KKIKERMTTS 

       190        200        210        220        230        240 
LPIARLSNRV FRDKLFPSLL KEHKNVVNVG PRNESMFTFL NYPTIKQFSN GAYLVKDTIK 

       250        260        270        280        290        300 
LKQERWLGKR ISQFDIGQYK NMLNVLTAIY YYYNLYKSKP IIYMIGSAPS YWIYDVRHYS 

       310        320        330        340        350        360 
DFFFETWDPL DTPYSSIHHK ELFFINDVKK LKDNSILYID IRTDRGNADW KKWRKTVEEQ 

       370        380        390        400        410        420 
TINNLDIAYE YLRTGKAKVC CVKMTAMHLE LPISAKLLHH PTTEIRSEFY LLLDTWDLTN 

       430        440        450        460        470        480 
IRRFIPKGVL YSFINNIITE NVFIQQPFKV KVLNDSYIVA LYALSNDFNN RSEVIKLINN 

       490        500        510        520        530        540 
QKQSLITVRI NNTFKDEPKV GFKNIYDWTF LPTDFDTKEA IITSYDGCLG LFGLSISLAS 

       550        560        570        580        590        600 
KPTGNNHLFI LSGTDKYYKL DQFANHTSIS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL 

       610        620        630        640        650        660 
IGTNIENSVS GHIYNALIYY RYNYSFDLKR WIYLHSIDKV DIEGGKYYEH APIELIYACR 

       670        680        690        700        710        720 
SAKEFATLQD DLTVLRYSNE IENYINTVYS ITYADDPNYF IGIQFRNIPY KYDVKIPHLT 

       730        740        750        760        770        780 
FGVLHISDNM VPDVIDILKI MKNELFKMDI TTSYTYMLSD GIYVANVSGV LSTYFKIYNV 

       790        800        810        820        830 
FYKNQITFGQ SRMFIPHITL SFNNMRTVRI ETTKLQIKSI YLRKIKGDTV FDMVE 

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References

[1]"Completion of the genomic sequence of the simian rotavirus SA11: nucleotide sequences of segments 1, 2, and 3."
Mitchell D.B., Both G.W.
Virology 177:324-331(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Nucleotide sequence of the simian rotavirus SA11 genome segment 3."
Liu M., Estes M.K.
Nucleic Acids Res. 17:7991-7991(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X16387 Genomic RNA. Translation: CAA34423.1.
X16062 Genomic RNA. Translation: CAA34198.1.
PIRP3XRSR. S06085.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011181. VP3_Rotav.
[Graphical view]
PfamPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVP3_ROTS1
AccessionPrimary (citable) accession number: P15736
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: August 1, 1991
Last modified: April 16, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families