Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphorylase b kinase gamma catalytic chain, liver/testis isoform

Gene

PHKG2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM (By similarity).By similarity1 Publication

Catalytic activityi

2 ATP + phosphorylase b = 2 ADP + phosphorylase a.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53ATPPROSITE-ProRule annotation1
Active sitei153Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 38ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorylase kinase activity Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB
  • tau-protein kinase activity Source: UniProtKB

GO - Biological processi

  • generation of precursor metabolites and energy Source: ProtInc
  • glycogen biosynthetic process Source: InterPro
  • glycogen catabolic process Source: Reactome
  • glycogen metabolic process Source: ProtInc
  • positive regulation of glycogen catabolic process Source: UniProtKB
  • protein phosphorylation Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS08155-MONOMER.
ZFISH:HS08155-MONOMER.
BRENDAi2.7.11.19. 2681.
ReactomeiR-HSA-70221. Glycogen breakdown (glycogenolysis).
SignaLinkiP15735.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase gamma catalytic chain, liver/testis isoform (EC:2.7.11.19)
Short name:
PHK-gamma-LT
Short name:
PHK-gamma-T
Alternative name(s):
PSK-C3
Phosphorylase kinase subunit gamma-2
Gene namesi
Name:PHKG2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:8931. PHKG2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • phosphorylase kinase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Involvement in diseasei

Glycogen storage disease 9C (GSD9C)3 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA metabolic disorder manifesting in infancy with hepatomegaly, growth retardation, hypotonia, liver dysfunction, and elevated plasma aminotransferases and lipids. These symptoms improve with age in most cases; however, some patients may develop hepatic fibrosis or cirrhosis.
See also OMIM:613027
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_009517106V → E in GSD9C. 1 PublicationCorresponds to variant rs137853589dbSNPEnsembl.1
Natural variantiVAR_020854157E → K in GSD9C. 1 PublicationCorresponds to variant rs752961445dbSNPEnsembl.1
Natural variantiVAR_009518189G → E in GSD9C. 1 PublicationCorresponds to variant rs137853588dbSNPEnsembl.1
Natural variantiVAR_020855215D → N in GSD9C. 1 PublicationCorresponds to variant rs767427889dbSNPEnsembl.1

Keywords - Diseasei

Disease mutation, Glycogen storage disease

Organism-specific databases

DisGeNETi5261.
MalaCardsiPHKG2.
MIMi613027. phenotype.
OpenTargetsiENSG00000156873.
Orphaneti264580. Glycogen storage disease due to liver phosphorylase kinase deficiency.
PharmGKBiPA33272.

Chemistry databases

ChEMBLiCHEMBL2349.
GuidetoPHARMACOLOGYi2147.

Polymorphism and mutation databases

BioMutaiPHKG2.
DMDMi125536.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865121 – 406Phosphorylase b kinase gamma catalytic chain, liver/testis isoformAdd BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei345PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP15735.
MaxQBiP15735.
PaxDbiP15735.
PeptideAtlasiP15735.
PRIDEiP15735.

PTM databases

iPTMnetiP15735.
PhosphoSitePlusiP15735.

Expressioni

Gene expression databases

BgeeiENSG00000156873.
CleanExiHS_PHKG2.
ExpressionAtlasiP15735. baseline and differential.
GenevisibleiP15735. HS.

Organism-specific databases

HPAiCAB073407.

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PHKA2P460194EBI-1383819,EBI-1642846

Protein-protein interaction databases

BioGridi111279. 53 interactors.
IntActiP15735. 31 interactors.
STRINGi9606.ENSP00000455607.

Chemistry databases

BindingDBiP15735.

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 23Combined sources11
Beta strandi24 – 32Combined sources9
Beta strandi34 – 43Combined sources10
Turni44 – 46Combined sources3
Beta strandi49 – 56Combined sources8
Helixi64 – 84Combined sources21
Beta strandi93 – 107Combined sources15
Helixi115 – 122Combined sources8
Helixi127 – 146Combined sources20
Helixi156 – 158Combined sources3
Beta strandi159 – 161Combined sources3
Beta strandi167 – 169Combined sources3
Helixi191 – 193Combined sources3
Helixi196 – 201Combined sources6
Helixi214 – 228Combined sources15
Helixi238 – 247Combined sources10
Helixi254 – 257Combined sources4
Beta strandi258 – 260Combined sources3
Helixi262 – 271Combined sources10
Turni276 – 278Combined sources3
Helixi282 – 287Combined sources6
Helixi289 – 291Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y7JX-ray2.50A/B/C/D6-293[»]
ProteinModelPortaliP15735.
SMRiP15735.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 291Protein kinasePROSITE-ProRule annotationAdd BLAST268

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni306 – 330Calmodulin-binding (domain-N)By similarityAdd BLAST25
Regioni346 – 370Calmodulin-binding (domain-C)By similarityAdd BLAST25

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0599. Eukaryota.
ENOG410Y7VX. LUCA.
GeneTreeiENSGT00840000129744.
HOGENOMiHOG000233016.
HOVERGENiHBG106193.
InParanoidiP15735.
KOiK00871.
PhylomeDBiP15735.
TreeFamiTF320349.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01049. PHOSPHBKNASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P15735-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLDVGPEDE LPDWAAAKEF YQKYDPKDVI GRGVSSVVRR CVHRATGHEF
60 70 80 90 100
AVKIMEVTAE RLSPEQLEEV REATRRETHI LRQVAGHPHI ITLIDSYESS
110 120 130 140 150
SFMFLVFDLM RKGELFDYLT EKVALSEKET RSIMRSLLEA VSFLHANNIV
160 170 180 190 200
HRDLKPENIL LDDNMQIRLS DFGFSCHLEP GEKLRELCGT PGYLAPEILK
210 220 230 240 250
CSMDETHPGY GKEVDLWACG VILFTLLAGS PPFWHRRQIL MLRMIMEGQY
260 270 280 290 300
QFSSPEWDDR SSTVKDLISR LLQVDPEARL TAEQALQHPF FERCEGSQPW
310 320 330 340 350
NLTPRQRFRV AVWTVLAAGR VALSTHRVRP LTKNALLRDP YALRSVRHLI
360 370 380 390 400
DNCAFRLYGH WVKKGEQQNR AALFQHRPPG PFPIMGPEEE GDSAAITEDE

AVLVLG
Length:406
Mass (Da):46,442
Last modified:April 1, 1990 - v1
Checksum:iE991CFF2D3D70F60
GO
Isoform 2 (identifier: P15735-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     362-374: VKKGEQQNRAALF → IRKQWIGKLMACV
     375-406: Missing.

Show »
Length:374
Mass (Da):43,158
Checksum:iD0E2416820A68AA7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146A → P in AAA36518 (PubMed:2948189).Curated1
Sequence conflicti176C → S in AAA36518 (PubMed:2948189).Curated1
Sequence conflicti179E → D in AAA36518 (PubMed:2948189).Curated1
Sequence conflicti212 – 214KEV → LVD in AAA36518 (PubMed:2948189).Curated3
Sequence conflicti221V → E in AAA36518 (PubMed:2948189).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_009517106V → E in GSD9C. 1 PublicationCorresponds to variant rs137853589dbSNPEnsembl.1
Natural variantiVAR_020854157E → K in GSD9C. 1 PublicationCorresponds to variant rs752961445dbSNPEnsembl.1
Natural variantiVAR_009518189G → E in GSD9C. 1 PublicationCorresponds to variant rs137853588dbSNPEnsembl.1
Natural variantiVAR_020855215D → N in GSD9C. 1 PublicationCorresponds to variant rs767427889dbSNPEnsembl.1
Natural variantiVAR_051658247E → G.Corresponds to variant rs34006569dbSNPEnsembl.1
Natural variantiVAR_040996317A → T.1 PublicationCorresponds to variant rs759992249dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041858362 – 374VKKGE…RAALF → IRKQWIGKLMACV in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_041859375 – 406Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31606 mRNA. Translation: AAA36442.1.
Y11950, Y11951 Genomic DNA. Translation: CAA72694.1.
AK289492 mRNA. Translation: BAF82181.1.
AK293551 mRNA. Translation: BAG57028.1.
AC106886 Genomic DNA. No translation available.
BC002541 mRNA. Translation: AAH02541.1.
M14503 mRNA. Translation: AAA36518.1.
CCDSiCCDS10690.1. [P15735-1]
CCDS54002.1. [P15735-2]
PIRiA40069. KIHUCT.
RefSeqiNP_000285.1. NM_000294.2. [P15735-1]
NP_001165903.1. NM_001172432.1. [P15735-2]
UniGeneiHs.65735.

Genome annotation databases

EnsembliENST00000424889; ENSP00000388571; ENSG00000156873. [P15735-2]
ENST00000563588; ENSP00000455607; ENSG00000156873. [P15735-1]
GeneIDi5261.
KEGGihsa:5261.
UCSCiuc021tgo.3. human. [P15735-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31606 mRNA. Translation: AAA36442.1.
Y11950, Y11951 Genomic DNA. Translation: CAA72694.1.
AK289492 mRNA. Translation: BAF82181.1.
AK293551 mRNA. Translation: BAG57028.1.
AC106886 Genomic DNA. No translation available.
BC002541 mRNA. Translation: AAH02541.1.
M14503 mRNA. Translation: AAA36518.1.
CCDSiCCDS10690.1. [P15735-1]
CCDS54002.1. [P15735-2]
PIRiA40069. KIHUCT.
RefSeqiNP_000285.1. NM_000294.2. [P15735-1]
NP_001165903.1. NM_001172432.1. [P15735-2]
UniGeneiHs.65735.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y7JX-ray2.50A/B/C/D6-293[»]
ProteinModelPortaliP15735.
SMRiP15735.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111279. 53 interactors.
IntActiP15735. 31 interactors.
STRINGi9606.ENSP00000455607.

Chemistry databases

BindingDBiP15735.
ChEMBLiCHEMBL2349.
GuidetoPHARMACOLOGYi2147.

PTM databases

iPTMnetiP15735.
PhosphoSitePlusiP15735.

Polymorphism and mutation databases

BioMutaiPHKG2.
DMDMi125536.

Proteomic databases

EPDiP15735.
MaxQBiP15735.
PaxDbiP15735.
PeptideAtlasiP15735.
PRIDEiP15735.

Protocols and materials databases

DNASUi5261.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000424889; ENSP00000388571; ENSG00000156873. [P15735-2]
ENST00000563588; ENSP00000455607; ENSG00000156873. [P15735-1]
GeneIDi5261.
KEGGihsa:5261.
UCSCiuc021tgo.3. human. [P15735-1]

Organism-specific databases

CTDi5261.
DisGeNETi5261.
GeneCardsiPHKG2.
GeneReviewsiPHKG2.
HGNCiHGNC:8931. PHKG2.
HPAiCAB073407.
MalaCardsiPHKG2.
MIMi172471. gene.
613027. phenotype.
neXtProtiNX_P15735.
OpenTargetsiENSG00000156873.
Orphaneti264580. Glycogen storage disease due to liver phosphorylase kinase deficiency.
PharmGKBiPA33272.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0599. Eukaryota.
ENOG410Y7VX. LUCA.
GeneTreeiENSGT00840000129744.
HOGENOMiHOG000233016.
HOVERGENiHBG106193.
InParanoidiP15735.
KOiK00871.
PhylomeDBiP15735.
TreeFamiTF320349.

Enzyme and pathway databases

BioCyciMetaCyc:HS08155-MONOMER.
ZFISH:HS08155-MONOMER.
BRENDAi2.7.11.19. 2681.
ReactomeiR-HSA-70221. Glycogen breakdown (glycogenolysis).
SignaLinkiP15735.

Miscellaneous databases

ChiTaRSiPHKG2. human.
GeneWikiiPHKG2.
GenomeRNAii5261.
PROiP15735.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000156873.
CleanExiHS_PHKG2.
ExpressionAtlasiP15735. baseline and differential.
GenevisibleiP15735. HS.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01049. PHOSPHBKNASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHKG2_HUMAN
AccessioniPrimary (citable) accession number: P15735
Secondary accession number(s): A8K0C7
, B4DEB7, E9PEU3, P11800
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 189 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.