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Protein

Ubiquitin-conjugating enzyme E2-16 kDa

Gene

UBC5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.

Miscellaneous

Present with 981 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • cellular response to heat Source: SGD
  • protein polyubiquitination Source: SGD

Keywordsi

Molecular functionTransferase
Biological processStress response, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29667-MONOMER
ReactomeiR-SCE-1169408 ISG15 antiviral mechanism
R-SCE-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-SCE-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-SCE-9033241 Peroxisomal protein import
R-SCE-936440 Negative regulators of DDX58/IFIH1 signaling
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-16 kDa (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 5
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:UBC5
Ordered Locus Names:YDR059C
ORF Names:D4234, YD9609.13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR059C
SGDiS000002466 UBC5

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825461 – 148Ubiquitin-conjugating enzyme E2-16 kDaAdd BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineBy similarity1
Cross-linki91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP15732
PaxDbiP15732
PRIDEiP15732

Expressioni

Inductioni

By heat shock and cadmium.

Interactioni

Protein-protein interaction databases

BioGridi32114, 67 interactors
IntActiP15732, 4 interactors
MINTiP15732
STRINGi4932.YDR059C

Structurei

3D structure databases

ProteinModelPortaliP15732
SMRiP15732
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00910000143990
HOGENOMiHOG000233455
InParanoidiP15732
KOiK06689
OMAiREWTANW
OrthoDBiEOG092C50OL

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P15732-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSKRIAKE LSDLGRDPPA SCSAGPVGDD LYHWQASIMG PSDSPYAGGV
60 70 80 90 100
FFLSIHFPTD YPFKPPKVNF TTKIYHPNIN SSGNICLDIL KDQWSPALTL
110 120 130 140
SKVLLSICSL LTDANPDDPL VPEIAQIYKT DKAKYEATAK EWTKKYAV
Length:148
Mass (Da):16,281
Last modified:April 1, 1990 - v1
Checksum:iDC72805EC1F819AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17494 Genomic DNA Translation: CAA35529.1
Z49209 Genomic DNA Translation: CAA89088.1
X84162 Genomic DNA Translation: CAA58975.1
Z74355 Genomic DNA Translation: CAA98877.1
BK006938 Genomic DNA Translation: DAA11905.1
PIRiS22858
RefSeqiNP_010344.1, NM_001180367.1

Genome annotation databases

EnsemblFungiiYDR059C; YDR059C; YDR059C
GeneIDi851631
KEGGisce:YDR059C

Similar proteinsi

Entry informationi

Entry nameiUBC5_YEAST
AccessioniPrimary (citable) accession number: P15732
Secondary accession number(s): D6VS45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: May 23, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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