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Protein

Ubiquitin-conjugating enzyme E2-16 kDa

Gene

UBC5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • cellular response to heat Source: SGD
  • protein polyubiquitination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Stress response, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29667-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-16 kDa (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 5
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:UBC5
Ordered Locus Names:YDR059C
ORF Names:D4234, YD9609.13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR059C.
SGDiS000002466. UBC5.

Subcellular locationi

GO - Cellular componenti

  • proteasome complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825461 – 148Ubiquitin-conjugating enzyme E2-16 kDaAdd BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineBy similarity1
Cross-linki91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP15732.
PRIDEiP15732.

Expressioni

Inductioni

By heat shock and cadmium.

Interactioni

Protein-protein interaction databases

BioGridi32114. 40 interactors.
IntActiP15732. 4 interactors.
MINTiMINT-2780403.

Structurei

3D structure databases

ProteinModelPortaliP15732.
SMRiP15732.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00860000134067.
HOGENOMiHOG000233455.
InParanoidiP15732.
KOiK06689.
OMAiQEIAREW.
OrthoDBiEOG092C50OL.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15732-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSKRIAKE LSDLGRDPPA SCSAGPVGDD LYHWQASIMG PSDSPYAGGV
60 70 80 90 100
FFLSIHFPTD YPFKPPKVNF TTKIYHPNIN SSGNICLDIL KDQWSPALTL
110 120 130 140
SKVLLSICSL LTDANPDDPL VPEIAQIYKT DKAKYEATAK EWTKKYAV
Length:148
Mass (Da):16,281
Last modified:April 1, 1990 - v1
Checksum:iDC72805EC1F819AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17494 Genomic DNA. Translation: CAA35529.1.
Z49209 Genomic DNA. Translation: CAA89088.1.
X84162 Genomic DNA. Translation: CAA58975.1.
Z74355 Genomic DNA. Translation: CAA98877.1.
BK006938 Genomic DNA. Translation: DAA11905.1.
PIRiS22858.
RefSeqiNP_010344.1. NM_001180367.1.

Genome annotation databases

EnsemblFungiiYDR059C; YDR059C; YDR059C.
GeneIDi851631.
KEGGisce:YDR059C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17494 Genomic DNA. Translation: CAA35529.1.
Z49209 Genomic DNA. Translation: CAA89088.1.
X84162 Genomic DNA. Translation: CAA58975.1.
Z74355 Genomic DNA. Translation: CAA98877.1.
BK006938 Genomic DNA. Translation: DAA11905.1.
PIRiS22858.
RefSeqiNP_010344.1. NM_001180367.1.

3D structure databases

ProteinModelPortaliP15732.
SMRiP15732.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32114. 40 interactors.
IntActiP15732. 4 interactors.
MINTiMINT-2780403.

Proteomic databases

MaxQBiP15732.
PRIDEiP15732.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR059C; YDR059C; YDR059C.
GeneIDi851631.
KEGGisce:YDR059C.

Organism-specific databases

EuPathDBiFungiDB:YDR059C.
SGDiS000002466. UBC5.

Phylogenomic databases

GeneTreeiENSGT00860000134067.
HOGENOMiHOG000233455.
InParanoidiP15732.
KOiK06689.
OMAiQEIAREW.
OrthoDBiEOG092C50OL.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-29667-MONOMER.

Miscellaneous databases

PROiP15732.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC5_YEAST
AccessioniPrimary (citable) accession number: P15732
Secondary accession number(s): D6VS45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 981 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.