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Protein

Ubiquitin-conjugating enzyme E2 4

Gene

UBC4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Mediates ubiquitination of PEX5.PROSITE-ProRule annotation1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Glycyl thioester intermediate1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein binding, bridging Source: SGD
  • ubiquitin binding Source: SGD
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • cellular response to heat Source: SGD
  • cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  • protein monoubiquitination Source: SGD
  • protein polyubiquitination Source: UniProtKB
  • protein ubiquitination Source: SGD
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Stress response, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29050-MONOMER.
ReactomeiR-SCE-1169408. ISG15 antiviral mechanism.
R-SCE-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 4 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 4
Ubiquitin carrier protein 4
Ubiquitin-protein ligase 4
Gene namesi
Name:UBC4
Ordered Locus Names:YBR082C
ORF Names:YBR0745
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR082C.
SGDiS000000286. UBC4.

Subcellular locationi

GO - Cellular componenti

  • proteasome complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825451 – 148Ubiquitin-conjugating enzyme E2 4Add BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineCombined sources1
Cross-linki91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP15731.
PRIDEiP15731.
TopDownProteomicsiP15731.

PTM databases

iPTMnetiP15731.

Expressioni

Inductioni

By heat shock and cadmium.

Interactioni

Subunit structurei

Interacts with TUL1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EGD2P388792EBI-19735,EBI-6379

GO - Molecular functioni

  • protein binding, bridging Source: SGD
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi32786. 267 interactors.
DIPiDIP-6596N.
IntActiP15731. 4 interactors.
MINTiMINT-677321.

Structurei

Secondary structure

1148
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 14Combined sources13
Beta strandi20 – 27Combined sources8
Beta strandi30 – 40Combined sources11
Beta strandi42 – 44Combined sources3
Turni45 – 48Combined sources4
Beta strandi50 – 56Combined sources7
Turni59 – 62Combined sources4
Beta strandi67 – 70Combined sources4
Helixi88 – 90Combined sources3
Turni91 – 93Combined sources3
Helixi100 – 112Combined sources13
Helixi122 – 130Combined sources9
Helixi132 – 146Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QCQX-ray2.70A1-148[»]
5AIEX-ray2.80B1-148[»]
ProteinModelPortaliP15731.
SMRiP15731.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15731.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
InParanoidiP15731.
KOiK06689.
OMAiYAGGVYF.
OrthoDBiEOG092C50OL.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15731-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSKRIAKE LSDLERDPPT SCSAGPVGDD LYHWQASIMG PADSPYAGGV
60 70 80 90 100
FFLSIHFPTD YPFKPPKISF TTKIYHPNIN ANGNICLDIL KDQWSPALTL
110 120 130 140
SKVLLSICSL LTDANPDDPL VPEIAHIYKT DRPKYEATAR EWTKKYAV
Length:148
Mass (Da):16,456
Last modified:April 1, 1990 - v1
Checksum:i8E96137D3EB20F80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17493 Genomic DNA. Translation: CAA35528.1.
X76294 Genomic DNA. Translation: CAA53942.1.
Z35951 Genomic DNA. Translation: CAA85027.1.
BK006936 Genomic DNA. Translation: DAA07201.1.
PIRiS22857.
RefSeqiNP_009638.1. NM_001178430.1.

Genome annotation databases

EnsemblFungiiYBR082C; YBR082C; YBR082C.
GeneIDi852376.
KEGGisce:YBR082C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17493 Genomic DNA. Translation: CAA35528.1.
X76294 Genomic DNA. Translation: CAA53942.1.
Z35951 Genomic DNA. Translation: CAA85027.1.
BK006936 Genomic DNA. Translation: DAA07201.1.
PIRiS22857.
RefSeqiNP_009638.1. NM_001178430.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QCQX-ray2.70A1-148[»]
5AIEX-ray2.80B1-148[»]
ProteinModelPortaliP15731.
SMRiP15731.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32786. 267 interactors.
DIPiDIP-6596N.
IntActiP15731. 4 interactors.
MINTiMINT-677321.

PTM databases

iPTMnetiP15731.

Proteomic databases

MaxQBiP15731.
PRIDEiP15731.
TopDownProteomicsiP15731.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR082C; YBR082C; YBR082C.
GeneIDi852376.
KEGGisce:YBR082C.

Organism-specific databases

EuPathDBiFungiDB:YBR082C.
SGDiS000000286. UBC4.

Phylogenomic databases

GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
InParanoidiP15731.
KOiK06689.
OMAiYAGGVYF.
OrthoDBiEOG092C50OL.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-29050-MONOMER.
ReactomeiR-SCE-1169408. ISG15 antiviral mechanism.
R-SCE-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Miscellaneous databases

EvolutionaryTraceiP15731.
PROiP15731.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC4_YEAST
AccessioniPrimary (citable) accession number: P15731
Secondary accession number(s): D6VQ81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 172 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.