Reviewed,
UniProtKB/Swiss-Prot P15731 (UBC4_YEAST)
Last modified
June 16, 2009.
Version 98.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ubiquitin-conjugating enzyme E2 4 EC=6.3.2.19 Alternative name(s): Ubiquitin-protein ligase 4 Ubiquitin carrier protein 4 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 148 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. |
| Catalytic activity | ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. |
| Pathway | |
| Subunit structure | Interacts with TUL1. Ref.4 |
| Induction | By heat shock and cadmium. |
| Post-translational modification | The N-terminus is blocked. |
| Miscellaneous | Present with 13500 molecules/cell in log phase SD medium. Ref.5 |
| Sequence similarities | Belongs to the ubiquitin-conjugating enzyme family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 148 | 148 | Ubiquitin-conjugating enzyme E2 4 | PRO_0000082545 | ||||||||||||||||||||||||||
Sites | ||||||||||||||||||||||||||||||
| Active site | 86 | 1 | Glycyl thioester intermediate | |||||||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||||||
| Modified residue | 12 | 1 | Phosphoserine Ref.6 | |||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||
| Helix | 2 – 14 | 13 | ||||||||||||||||||||||||||||
| Beta strand | 20 – 27 | 8 | ||||||||||||||||||||||||||||
| Beta strand | 30 – 40 | 11 | ||||||||||||||||||||||||||||
| Beta strand | 50 – 56 | 7 | ||||||||||||||||||||||||||||
| Turn | 59 – 62 | 4 | ||||||||||||||||||||||||||||
| Beta strand | 67 – 70 | 4 | ||||||||||||||||||||||||||||
| Helix | 88 – 90 | 3 | ||||||||||||||||||||||||||||
| Turn | 91 – 93 | 3 | ||||||||||||||||||||||||||||
| Helix | 100 – 112 | 13 | ||||||||||||||||||||||||||||
| Helix | 122 – 130 | 9 | ||||||||||||||||||||||||||||
| Helix | 132 – 146 | 15 | ||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins." Seufert W., Jentsch S. EMBO J. 9:543-550(1990) [PubMed: 2154373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-64 AND 119-125. |
| [2] | "Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II." van der Aart Q.J.M., Barthe C., Doignon F., Aigle M., Crouzet M., Steensma H.Y. Yeast 10:959-964(1994) [PubMed: 7985423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K.EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "A transmembrane ubiquitin ligase required to sort membrane proteins into multivesicular bodies." Reggiori F., Pelham H.R.B. Nat. Cell Biol. 4:117-123(2002) [PubMed: 11788821] [Abstract] Cited for: INTERACTION WITH TUL1. |
| [5] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [6] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, MASS SPECTROMETRY. |
| [7] | "Tertiary structures of class I ubiquitin-conjugating enzymes are highly conserved: crystal structure of yeast Ubc4." Cook W.J., Jeffrey L.C., Xu Y., Chau V. Biochemistry 32:13809-13817(1993) [PubMed: 8268156] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS). |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X17493 Genomic DNA. Translation: CAA35528.1. X76294 Genomic DNA. Translation: CAA53942.1. Z35951 Genomic DNA. Translation: CAA85027.1. | |||||||||||||
| PIR | S22857. | ||||||||||||
| RefSeq | NP_009638.1. | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| DIP | DIP:6596N. | ||||||||||||
| IntAct | P15731. 15 interactions. | ||||||||||||
Proteomic databases | |||||||||||||
| PeptideAtlas | P15731. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | YBR082C. Saccharomyces cerevisiae. [Contig view] | ||||||||||||
| GeneID | 852376. | ||||||||||||
| GenomeReviews | Gene locus YBR082C in contig Y13134_GR. | ||||||||||||
| KEGG | sce:YBR082C. | ||||||||||||
| NMPDR | fig|4932.3.peg.336. | ||||||||||||
Organism-specific databases | |||||||||||||
| CYGD | YBR082c. | ||||||||||||
| SGD | S000000286. UBC4. | ||||||||||||
| Yeast-GFP | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | P15731. | ||||||||||||
| OMA | P15731. NFTTRIY. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BRENDA | 6.3.2.19. 250. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P15731. | ||||||||||||
| GermOnline | YBR082C. Saccharomyces cerevisiae. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit. | ||||||||||||
| Pfam | PF00179. UQ_con. 1 hit. [Graphical view] | ||||||||||||
| ProDom | PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||
| SMART | SM00212. UBCc. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 971168. | ||||||||||||
Entry information
| Entry name | UBC4_YEAST | ||||||||
| Accession | Primary (citable) accession number: P15731 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome II Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names |

Clusters with


