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Protein

Ubiquitin-conjugating enzyme E2 4

Gene

UBC4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Mediates ubiquitination of PEX5.PROSITE-ProRule annotation1 Publication

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Glycyl thioester intermediate1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein binding, bridging Source: SGD
  • ubiquitin binding Source: SGD
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • cellular response to heat Source: SGD
  • cytoplasm protein quality control by the ubiquitin-proteasome system Source: SGD
  • protein monoubiquitination Source: SGD
  • protein polyubiquitination Source: SGD
  • protein ubiquitination Source: SGD
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD

Keywordsi

Molecular functionTransferase
Biological processStress response, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29050-MONOMER
ReactomeiR-SCE-1169408 ISG15 antiviral mechanism
R-SCE-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 4 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 4
Ubiquitin carrier protein 4
Ubiquitin-protein ligase 4
Gene namesi
Name:UBC4
Ordered Locus Names:YBR082C
ORF Names:YBR0745
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR082C
SGDiS000000286 UBC4

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825451 – 148Ubiquitin-conjugating enzyme E2 4Add BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineCombined sources1
Cross-linki91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP15731
PaxDbiP15731
PRIDEiP15731
TopDownProteomicsiP15731

PTM databases

iPTMnetiP15731

Expressioni

Inductioni

By heat shock and cadmium.

Interactioni

Subunit structurei

Interacts with TUL1.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein binding, bridging Source: SGD
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi32786, 405 interactors
DIPiDIP-6596N
IntActiP15731, 14 interactors
MINTiP15731
STRINGi4932.YBR082C

Structurei

Secondary structure

1148
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 14Combined sources13
Beta strandi20 – 27Combined sources8
Beta strandi30 – 40Combined sources11
Beta strandi42 – 44Combined sources3
Turni45 – 48Combined sources4
Beta strandi50 – 56Combined sources7
Turni59 – 62Combined sources4
Beta strandi67 – 70Combined sources4
Helixi88 – 90Combined sources3
Turni91 – 93Combined sources3
Helixi100 – 112Combined sources13
Helixi122 – 130Combined sources9
Helixi132 – 146Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QCQX-ray2.70A1-148[»]
5AIEX-ray2.80B1-148[»]
ProteinModelPortaliP15731
SMRiP15731
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15731

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00910000143990
HOGENOMiHOG000233455
InParanoidiP15731
KOiK06689
OMAiNDLGRDP
OrthoDBiEOG092C50OL

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P15731-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSKRIAKE LSDLERDPPT SCSAGPVGDD LYHWQASIMG PADSPYAGGV
60 70 80 90 100
FFLSIHFPTD YPFKPPKISF TTKIYHPNIN ANGNICLDIL KDQWSPALTL
110 120 130 140
SKVLLSICSL LTDANPDDPL VPEIAHIYKT DRPKYEATAR EWTKKYAV
Length:148
Mass (Da):16,456
Last modified:April 1, 1990 - v1
Checksum:i8E96137D3EB20F80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17493 Genomic DNA Translation: CAA35528.1
X76294 Genomic DNA Translation: CAA53942.1
Z35951 Genomic DNA Translation: CAA85027.1
BK006936 Genomic DNA Translation: DAA07201.1
PIRiS22857
RefSeqiNP_009638.1, NM_001178430.1

Genome annotation databases

EnsemblFungiiYBR082C; YBR082C; YBR082C
GeneIDi852376
KEGGisce:YBR082C

Similar proteinsi

Entry informationi

Entry nameiUBC4_YEAST
AccessioniPrimary (citable) accession number: P15731
Secondary accession number(s): D6VQ81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: March 28, 2018
This is version 185 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health