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Protein

Deoxyguanosinetriphosphate triphosphohydrolase

Gene

dgt

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

dGTPase preferentially hydrolyzes dGTP over the other canonical NTPs.UniRule annotation1 Publication

Catalytic activityi

dGTP + H2O = deoxyguanosine + triphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 Publication

GO - Molecular functioni

  • cobalt ion binding Source: EcoliWiki
  • dGTPase activity Source: EcoCyc
  • GTPase activity Source: EcoliWiki
  • magnesium ion binding Source: UniProtKB-HAMAP
  • manganese ion binding Source: EcoliWiki
  • single-stranded DNA binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciEcoCyc:DGTPTRIPHYDRO-MONOMER.
ECOL316407:JW0156-MONOMER.
MetaCyc:DGTPTRIPHYDRO-MONOMER.
SABIO-RKP15723.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyguanosinetriphosphate triphosphohydrolaseUniRule annotation (EC:3.1.5.1UniRule annotation)
Short name:
dGTP triphosphohydrolaseUniRule annotation
Short name:
dGTPaseUniRule annotation
Gene namesi
Name:dgtUniRule annotation
Ordered Locus Names:b0160, JW0156
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10225. dgt.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002052792 – 505Deoxyguanosinetriphosphate triphosphohydrolaseAdd BLAST504

Proteomic databases

PaxDbiP15723.
PRIDEiP15723.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4260993. 6 interactors.
DIPiDIP-9437N.
IntActiP15723. 5 interactors.
MINTiMINT-1223226.
STRINGi511145.b0160.

Structurei

Secondary structure

1505
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Beta strandi14 – 17Combined sources4
Helixi25 – 40Combined sources16
Helixi43 – 49Combined sources7
Beta strandi51 – 55Combined sources5
Helixi66 – 90Combined sources25
Helixi95 – 97Combined sources3
Helixi103 – 115Combined sources13
Helixi118 – 120Combined sources3
Turni123 – 125Combined sources3
Helixi126 – 140Combined sources15
Helixi142 – 145Combined sources4
Beta strandi146 – 148Combined sources3
Helixi158 – 160Combined sources3
Helixi168 – 180Combined sources13
Helixi185 – 194Combined sources10
Helixi203 – 209Combined sources7
Helixi224 – 226Combined sources3
Turni227 – 230Combined sources4
Beta strandi231 – 235Combined sources5
Helixi240 – 249Combined sources10
Helixi260 – 282Combined sources23
Helixi288 – 299Combined sources12
Helixi307 – 320Combined sources14
Helixi328 – 354Combined sources27
Helixi356 – 360Combined sources5
Beta strandi371 – 373Combined sources3
Helixi374 – 389Combined sources16
Turni390 – 392Combined sources3
Helixi394 – 419Combined sources26
Helixi422 – 431Combined sources10
Helixi438 – 446Combined sources9
Helixi449 – 460Combined sources12
Helixi469 – 486Combined sources18
Helixi490 – 500Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4X9EX-ray3.10A/B/C/D/E/F1-505[»]
4XDSX-ray3.35A/B/C/D/E/F1-505[»]
ProteinModelPortaliP15723.
SMRiP15723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the dGTPase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4Q. Bacteria.
COG0232. LUCA.
HOGENOMiHOG000082150.
InParanoidiP15723.
KOiK01129.
OMAiKTDFVHT.
PhylomeDBiP15723.

Family and domain databases

Gene3Di1.10.3210.10. 2 hits.
1.10.3410.10. 1 hit.
1.10.3550.10. 2 hits.
HAMAPiMF_00030. dGTPase_type1. 1 hit.
InterProiIPR006261. dGTP_triP_hydro.
IPR023293. dGTP_triP_hydro_central.
IPR020779. dGTP_triP_hydro_proteobacteria.
IPR027432. dGTP_triphosphohydrolase_C_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
[Graphical view]
PfamiPF01966. HD. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01353. dGTP_triPase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15723-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQIDFRKKI NWHRRYRSPQ GVKTEHEILR IFESDRGRII NSPAIRRLQQ
60 70 80 90 100
KTQVFPLERN AAVRTRLTHS MEVQQVGRYI AKEILSRLKE LKLLEAYGLD
110 120 130 140 150
ELTGPFESIV EMSCLMHDIG NPPFGHFGEA AINDWFRQRL HPEDAESQPL
160 170 180 190 200
TDDRCSVAAL RLRDGEEPLN ELRRKIRQDL CHFEGNAQGI RLVHTLMRMN
210 220 230 240 250
LTWAQVGGIL KYTRPAWWRG ETPETHHYLM KKPGYYLSEE AYIARLRKEL
260 270 280 290 300
NLALYSRFPL TWIMEAADDI SYCVADLEDA VEKRIFTVEQ LYHHLHEAWG
310 320 330 340 350
QHEKGSLFSL VVENAWEKSR SNSLSRSTED QFFMYLRVNT LNKLVPYAAQ
360 370 380 390 400
RFIDNLPAIF AGTFNHALLE DASECSDLLK LYKNVAVKHV FSHPDVERLE
410 420 430 440 450
LQGYRVISGL LEIYRPLLSL SLSDFTELVE KERVKRFPIE SRLFHKLSTR
460 470 480 490 500
HRLAYVEAVS KLPSDSPEFP LWEYYYRCRL LQDYISGMTD LYAWDEYRRL

MAVEQ
Length:505
Mass (Da):59,383
Last modified:January 23, 2007 - v4
Checksum:iB04C4CA02AD5B16E
GO

Sequence cautioni

The sequence AAA23716 differs from that shown. Reason: Frameshift at positions 90 and 211.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti363T → R in AAA23716 (PubMed:2165018).Curated1
Sequence conflicti450R → P in AAA23716 (PubMed:2165018).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31772 Genomic DNA. Translation: AAA23679.1.
M29955 Genomic DNA. Translation: AAA23716.1. Frameshift.
U70214 Genomic DNA. Translation: AAB08590.1.
U00096 Genomic DNA. Translation: AAC73271.1.
AP009048 Genomic DNA. Translation: BAB96737.1.
PIRiA35993.
RefSeqiNP_414702.1. NC_000913.3.
WP_000057073.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73271; AAC73271; b0160.
BAB96737; BAB96737; BAB96737.
GeneIDi947177.
KEGGiecj:JW0156.
eco:b0160.
PATRICi32115429. VBIEscCol129921_0166.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31772 Genomic DNA. Translation: AAA23679.1.
M29955 Genomic DNA. Translation: AAA23716.1. Frameshift.
U70214 Genomic DNA. Translation: AAB08590.1.
U00096 Genomic DNA. Translation: AAC73271.1.
AP009048 Genomic DNA. Translation: BAB96737.1.
PIRiA35993.
RefSeqiNP_414702.1. NC_000913.3.
WP_000057073.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4X9EX-ray3.10A/B/C/D/E/F1-505[»]
4XDSX-ray3.35A/B/C/D/E/F1-505[»]
ProteinModelPortaliP15723.
SMRiP15723.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260993. 6 interactors.
DIPiDIP-9437N.
IntActiP15723. 5 interactors.
MINTiMINT-1223226.
STRINGi511145.b0160.

Proteomic databases

PaxDbiP15723.
PRIDEiP15723.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73271; AAC73271; b0160.
BAB96737; BAB96737; BAB96737.
GeneIDi947177.
KEGGiecj:JW0156.
eco:b0160.
PATRICi32115429. VBIEscCol129921_0166.

Organism-specific databases

EchoBASEiEB0221.
EcoGeneiEG10225. dgt.

Phylogenomic databases

eggNOGiENOG4105C4Q. Bacteria.
COG0232. LUCA.
HOGENOMiHOG000082150.
InParanoidiP15723.
KOiK01129.
OMAiKTDFVHT.
PhylomeDBiP15723.

Enzyme and pathway databases

BioCyciEcoCyc:DGTPTRIPHYDRO-MONOMER.
ECOL316407:JW0156-MONOMER.
MetaCyc:DGTPTRIPHYDRO-MONOMER.
SABIO-RKP15723.

Miscellaneous databases

PROiP15723.

Family and domain databases

Gene3Di1.10.3210.10. 2 hits.
1.10.3410.10. 1 hit.
1.10.3550.10. 2 hits.
HAMAPiMF_00030. dGTPase_type1. 1 hit.
InterProiIPR006261. dGTP_triP_hydro.
IPR023293. dGTP_triP_hydro_central.
IPR020779. dGTP_triP_hydro_proteobacteria.
IPR027432. dGTP_triphosphohydrolase_C_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
[Graphical view]
PfamiPF01966. HD. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01353. dGTP_triPase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDGTP_ECOLI
AccessioniPrimary (citable) accession number: P15723
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 153 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.