P15723 (DGTP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyguanosinetriphosphate triphosphohydrolase
Short name=dGTP triphosphohydrolase
Short name=dGTPase
EC=3.1.5.1

Gene names

Name:	dgt
Ordered Locus Names:	b0160, JW0156

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	dGTPase preferentially hydrolyzes dGTP over the other canonical NTPs. HAMAP MF_00030
Catalytic activity	dGTP + H2O = deoxyguanosine + triphosphate. HAMAP MF_00030
Cofactor	Magnesium.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the dGTPase family. Type 1 subfamily.
Sequence caution	The sequence AAA23716.1 differs from that shown. Reason: Frameshift at positions 90 and 211.

Ontologies

Keywords
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	GTPase activity Inferred from direct assay Ref.7. Source: EcoliWiki cobalt ion binding Inferred from direct assay Ref.7. Source: EcoliWiki dGTPase activity Inferred from direct assay. Source: EcoliWiki magnesium ion binding Inferred from direct assay Ref.7. Source: EcoliWiki manganese ion binding Inferred from direct assay Ref.7. Source: EcoliWiki phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7
	Chain	2 – 505	504	Deoxyguanosinetriphosphate triphosphohydrolase HAMAP MF_00030		PRO_0000205279
Experimental info
	Sequence conflict	363	1	T → R in AAA23716. Ref.2
	Sequence conflict	450	1	R → P in AAA23716. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P15723 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: B04C4CA02AD5B16E	FASTA	505	59,383
	10 20 30 40 50 60 MAQIDFRKKI NWHRRYRSPQ GVKTEHEILR IFESDRGRII NSPAIRRLQQ KTQVFPLERN 70 80 90 100 110 120 AAVRTRLTHS MEVQQVGRYI AKEILSRLKE LKLLEAYGLD ELTGPFESIV EMSCLMHDIG 130 140 150 160 170 180 NPPFGHFGEA AINDWFRQRL HPEDAESQPL TDDRCSVAAL RLRDGEEPLN ELRRKIRQDL 190 200 210 220 230 240 CHFEGNAQGI RLVHTLMRMN LTWAQVGGIL KYTRPAWWRG ETPETHHYLM KKPGYYLSEE 250 260 270 280 290 300 AYIARLRKEL NLALYSRFPL TWIMEAADDI SYCVADLEDA VEKRIFTVEQ LYHHLHEAWG 310 320 330 340 350 360 QHEKGSLFSL VVENAWEKSR SNSLSRSTED QFFMYLRVNT LNKLVPYAAQ RFIDNLPAIF 370 380 390 400 410 420 AGTFNHALLE DASECSDLLK LYKNVAVKHV FSHPDVERLE LQGYRVISGL LEIYRPLLSL 430 440 450 460 470 480 SLSDFTELVE KERVKRFPIE SRLFHKLSTR HRLAYVEAVS KLPSDSPEFP LWEYYYRCRL 490 500 LQDYISGMTD LYAWDEYRRL MAVEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and regulation of the gene for dGTP triphosphohydrolase from Escherichia coli." Wurgler S.M., Richardson C.C. Proc. Natl. Acad. Sci. U.S.A. 87:2740-2744(1990) [PubMed: 2157212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HR42 and HR44.
[2]	"Primary structure of the deoxyguanosine triphosphate triphosphohydrolase-encoding gene (dgt) of Escherichia coli." Quirk S., Bhatnagar S.K., Bessman M.J. Gene 89:13-18(1990) [PubMed: 2165018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"The purification and properties of deoxyguanosine triphosphate triphosphohydrolase from Escherichia coli." Seto D., Bhatnagar S.K., Bessman M.J. J. Biol. Chem. 263:1494-1499(1988) [PubMed: 2826481] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31772 Genomic DNA. Translation: AAA23679.1. M29955 Genomic DNA. Translation: AAA23716.1. Frameshift. U70214 Genomic DNA. Translation: AAB08590.1. U00096 Genomic DNA. Translation: AAC73271.1. AP009048 Genomic DNA. Translation: BAB96737.1.
PIR	A35993.
RefSeq	NP_414702.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P15723.
SMR	P15723. Positions 3-500.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9437N.
IntAct	P15723. 5 interactions.
MINT	MINT-1223226.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004512; EBESCP00000004512; EBESCG00000003686. EBESCT00000004513; EBESCP00000004513; EBESCG00000003686. EBESCT00000016880; EBESCP00000016171; EBESCG00000015939.
GeneID	947177.
GenomeReviews	Gene locus JW0156 in contig AP009048_GR. Gene locus b0160 in contig U00096_GR.
KEGG	ecj:JW0156. eco:b0160.
PATRIC	32115429. VBIEscCol129921_0166.
Organism-specific databases
EchoBASE	EB0221.
EcoGene	EG10225. dgt.
Phylogenomic databases
eggNOG	COG0232.
GeneTree	EBGT00050000009116.
HOGENOM	HBG470926.
OMA	LVEMACL.
PhylomeDB	P15723.
ProtClustDB	PRK04926.
Enzyme and pathway databases
BioCyc	EcoCyc:DGTPTRIPHYDRO-MONOMER. MetaCyc:DGTPTRIPHYDRO-MONOMER.
Gene expression databases
Genevestigator	P15723.
Family and domain databases
HAMAP	MF_00030. dGTPase_type1. [Tree]
InterPro	IPR006261. dGTP_triP_hydro. IPR023293. dGTP_triP_hydro_central. IPR020779. dGTP_triP_hydro_proteobacteria. IPR023279. HD. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR006674. Metal-dep_PHydrolase_HD_sub. [Graphical view]
Gene3D	G3DSA:1.10.3410.10. G3DSA:1.10.3410.10. 1 hit. G3DSA:1.10.3210.10. HD. 2 hits.
KO	K01129.
Pfam	PF01966. HD. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01353. DGTP_triPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DGTP_ECOLI

Accession

Primary (citable) accession number: P15723

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 113 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program