Reviewed, UniProtKB/Swiss-Prot P15723 (DGTP_ECOLI)

Names and origin

Protein names

Recommended name:
    Deoxyguanosinetriphosphate triphosphohydrolase
      Short name=dGTPase
      Short name=dGTP triphosphohydrolase
    EC=3.1.5.1

Gene names

Name:	dgt
Ordered Locus Names:	b0160, JW0156

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	dGTPase preferentially hydrolyzes dGTP over the other canonical NTPs. HAMAP MF_00030
Catalytic activity	dGTP + H2O = deoxyguanosine + triphosphate. HAMAP MF_00030
Cofactor	Magnesium. HAMAP MF_00030
Subunit structure	Homotetramer. HAMAP MF_00030
Sequence similarities	Belongs to the dGTPase family. Type 1 subfamily.
Sequence caution	The sequence AAA23716.1 differs from that shown. Reason: Frameshift at positions 90 and 211.

Ontologies

Keywords
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	GTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dGTPase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7
	Chain	2 – 505	504	Deoxyguanosinetriphosphate triphosphohydrolase HAMAP MF_00030		PRO_0000205279
Experimental info
	Sequence conflict	363	1	T → R in AAA23716. Ref.2
	Sequence conflict	450	1	R → P in AAA23716. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P15723-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: B04C4CA02AD5B16E	FASTA	505	59,383
	10 20 30 40 50 60 MAQIDFRKKI NWHRRYRSPQ GVKTEHEILR IFESDRGRII NSPAIRRLQQ KTQVFPLERN 70 80 90 100 110 120 AAVRTRLTHS MEVQQVGRYI AKEILSRLKE LKLLEAYGLD ELTGPFESIV EMSCLMHDIG 130 140 150 160 170 180 NPPFGHFGEA AINDWFRQRL HPEDAESQPL TDDRCSVAAL RLRDGEEPLN ELRRKIRQDL 190 200 210 220 230 240 CHFEGNAQGI RLVHTLMRMN LTWAQVGGIL KYTRPAWWRG ETPETHHYLM KKPGYYLSEE 250 260 270 280 290 300 AYIARLRKEL NLALYSRFPL TWIMEAADDI SYCVADLEDA VEKRIFTVEQ LYHHLHEAWG 310 320 330 340 350 360 QHEKGSLFSL VVENAWEKSR SNSLSRSTED QFFMYLRVNT LNKLVPYAAQ RFIDNLPAIF 370 380 390 400 410 420 AGTFNHALLE DASECSDLLK LYKNVAVKHV FSHPDVERLE LQGYRVISGL LEIYRPLLSL 430 440 450 460 470 480 SLSDFTELVE KERVKRFPIE SRLFHKLSTR HRLAYVEAVS KLPSDSPEFP LWEYYYRCRL 490 500 LQDYISGMTD LYAWDEYRRL MAVEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and regulation of the gene for dGTP triphosphohydrolase from Escherichia coli." Wurgler S.M., Richardson C.C. Proc. Natl. Acad. Sci. U.S.A. 87:2740-2744(1990) [PubMed: 2157212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HR42 and HR44.
[2]	"Primary structure of the deoxyguanosine triphosphate triphosphohydrolase-encoding gene (dgt) of Escherichia coli." Quirk S., Bhatnagar S.K., Bessman M.J. Gene 89:13-18(1990) [PubMed: 2165018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"The purification and properties of deoxyguanosine triphosphate triphosphohydrolase from Escherichia coli." Seto D., Bhatnagar S.K., Bessman M.J. J. Biol. Chem. 263:1494-1499(1988) [PubMed: 2826481] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M31772 Genomic DNA. Translation: AAA23679.1. M29955 Genomic DNA. Translation: AAA23716.1. Frameshift. U70214 Genomic DNA. Translation: AAB08590.1. U00096 Genomic DNA. Translation: AAC73271.1. AP009048 Genomic DNA. Translation: BAB96737.1.
PIR	A35993.
RefSeq	AP_000821.1. NP_414702.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9437N.
STRING	P15723.
Genome annotation databases
GeneID	947177.
GenomeReviews	Gene locus JW0156 in contig AP009048_GR. Gene locus b0160 in contig U00096_GR.
KEGG	ecj:JW0156. eco:b0160.
Organism-specific databases
EchoBASE	EB0221.
EcoGene	EG10225. dgt.
CMR	Search...
Phylogenomic databases
HOGENOM	P15723.
OMA	ELQGYRI
Enzyme and pathway databases
BioCyc	EcoCyc:DGTPTRIPHYDRO-MON. ECOL168927:B0160-MON. MetaCyc:DGTPTRIPHYDRO-MON.
Gene expression databases
Genevestigator	P15723.
Family and domain databases
HAMAP	MF_00030. [Tree]
InterPro	IPR006261. dGTP_triP_hydro. IPR020779. dGTP_triP_hydro_proteobacteria. IPR003607. Met-dep_phosphohydro_HD. IPR006674. Met-dep_phosphohydro_HD_sub. [Graphical view]
Pfam	PF01966. HD. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01353. dGTP_triPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DGTP_ECOLI

Accession

Primary (citable) accession number: P15723

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 92 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)