P15722 (PLY59_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable pectate lyase P59
EC=4.2.2.2

Gene names

Name:

LAT59

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Might be needed during pollen development and tube growth.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Tissue specificity	Expressed in anthers and pollen.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 449	427	Probable pectate lyase P59		PRO_0000024891
Sites
	Active site	325	1	Potential
	Metal binding	245	1	Calcium By similarity
	Metal binding	269	1	Calcium By similarity
	Metal binding	273	1	Calcium By similarity
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Glycosylation	80	1	N-linked (GlcNAc...) Potential
	Glycosylation	81	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P15722 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 17E3AA13F173B03C	FASTA	449	50,893
	10 20 30 40 50 60 MGGPKIKYSF LFLCITFATI IPSLMAHIGH YDEVWRRRAE EAKEYARNIY EPHPENVTLA 70 80 90 100 110 120 FNQKLRDTMK ELKKVKGTHN NSTRRGLGTK KYTGPCMVTN PIDKCWRCDP NWADNRKKLA 130 140 150 160 170 180 DCAMGFGSKA IGGKDGEFYV VTDNSDDYND PKPGTLRHAV IQKEPLWIIF KRGMNIRLHQ 190 200 210 220 230 240 EMIMQSDKTI DARGVNVHIT KGAGITLQYI KNVIIHGLHI HDIVEGNGGM VRDAVDHIGI 250 260 270 280 290 300 RTKSDGDGIS IFGASYIWID HVSMQRCYDG LIDAVEGSTG ITISNGHFTD HNEVMLFGAS 310 320 330 340 350 360 DSSSIDQVMQ ITLAFNHFGK RLIQRMPRCR WGYIHVVNND YTHWNMYAIG GSMHPTIIHQ 370 380 390 400 410 420 GNRFIAPPDI FKKQVTKREY NPESVWMQWT WRSEGNLFMN GAYFTESGDP EWSSKHKDLY 430 440 DGISAAPAED VTWMTRFAGV LGCKPGKPC

References

[1]

"Molecular and genetic characterization of two pollen-expressed genes that have sequence similarity to pectate lyases of the plant pathogen Erwinia." Wing R.A., Yamaguchi J., Larabell S.K., Ursin V.M., McCormick S. Plant Mol. Biol. 14:17-28(1990) [PubMed: 1983191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. VF36. Tissue: Anther.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15499 Genomic DNA. Translation: CAA33523.1.
PIR	S27098.
3D structure databases
ProteinModelPortal	P15722.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR007524. Pec_lyase_N. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. PF04431. Pec_lyase_N. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLY59_SOLLC

Accession

Primary (citable) accession number: P15722

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	September 21, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program