P15721 (PLY56_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable pectate lyase P56
EC=4.2.2.2

Gene names

Name:

LAT56

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Might be needed during pollen development and tube growth.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Tissue specificity	Expressed in anthers and pollen.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 398	371	Probable pectate lyase P56		PRO_0000024890
Sites
	Active site	273	1	Potential
	Metal binding	192	1	Calcium By similarity
	Metal binding	217	1	Calcium By similarity
	Metal binding	221	1	Calcium By similarity
Amino acid modifications
	Glycosylation	135	1	N-linked (GlcNAc...) Potential
	Glycosylation	228	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P15721 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 8D676250BD8BC7C8	FASTA	398	44,564
	10 20 30 40 50 60 MEYSYRTKIN VLFIVLILFV FAALGTAINA PRRKLTKKYR GPCMAVNSID KCWRCDPFWA 70 80 90 100 110 120 EDRQKMADCA LGFGINAMGG KYGPYYIVTD NSDDDVVDPK PGTLRFGVIQ KGPLWITFAR 130 140 150 160 170 180 SMRIRLTREL IVSSNKTIDG RGKYVHIANG AGIKIQSASN VIISNLRIHN IVPTAGGLLR 190 200 210 220 230 240 ESDDHLGLRG ADEGDAISIF NSHDIWIDHI SMSRATDGLI DAVAGSTNIT ISNCHFTDHE 250 260 270 280 290 300 KVMLFGANDH AEEDRGMKIT LAYNHFGKRL DQRMPRCRFG FFHLVNNDYT HWERYAIGGS 310 320 330 340 350 360 SGATIISQGN RFIAEDKLLV KEVTYREKST SSVEEWMKWT WITDGDDFEN GATFTPSGDQ 370 380 390 NLLSKIDHLN LIQPEPSSKV GLLTKFSGAL SCKIRRPC

References

[1]	"Molecular and genetic characterization of two pollen-expressed genes that have sequence similarity to pectate lyases of the plant pathogen Erwinia." Wing R.A., Yamaguchi J., Larabell S.K., Ursin V.M., McCormick S. Plant Mol. Biol. 14:17-28(1990) [PubMed: 1983191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. VF36. Tissue: Anther.
[2]	Wing R.A. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15500 Genomic DNA. Translation: CAA33524.1.
PIR	T07058.
3D structure databases
ProteinModelPortal	P15721.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLY56_SOLLC

Accession

Primary (citable) accession number: P15721

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	February 1, 1996
Last modified:	September 21, 2011
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program