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P15719

- MDHP_MAIZE

UniProt

P15719 - MDHP_MAIZE

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Protein
Malate dehydrogenase [NADP], chloroplastic
Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

Catalytic activityi

(S)-malate + NADP+ = oxaloacetate + NADPH.

Enzyme regulationi

Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei67 – 671Activation of NADP-MDH
Sitei72 – 721Activation of NADP-MDH
Binding sitei177 – 1771Substrate By similarity
Binding sitei183 – 1831Substrate By similarity
Binding sitei190 – 1901NADP By similarity
Binding sitei197 – 1971NAD By similarity
Binding sitei216 – 2161Substrate By similarity
Binding sitei247 – 2471Substrate By similarity
Active sitei272 – 2721Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi96 – 1027NADP By similarity
Nucleotide bindingi214 – 2163NADP By similarity

GO - Molecular functioni

  1. malate dehydrogenase (NADP+) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.82. 6752.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase [NADP], chloroplastic (EC:1.1.1.82)
Alternative name(s):
NADP-MDH
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiP15719.
MaizeGDBi40092.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: EnsemblPlants/Gramene
  2. chloroplast envelope Source: EnsemblPlants/Gramene
  3. chloroplast stroma Source: EnsemblPlants/Gramene
  4. mitochondrion Source: EnsemblPlants/Gramene
  5. thylakoid Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4040Chloroplast1 Publication
Add
BLAST
Chaini41 – 432392Malate dehydrogenase [NADP], chloroplastic
PRO_0000018643Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi67 ↔ 72In oxidized inactive NAD-MDH1 Publication
Disulfide bondi408 ↔ 420In oxidized inactive NAD-MDH By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP15719.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP15719.
SMRiP15719. Positions 64-429.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000220953.
KOiK00051.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15719-1 [UniParc]FASTAAdd to Basket

« Hide

MGLSTVYSPA GPRLVPAPLG RCRSAQPRRP RRAPLATVRC SVDATKQAQD    50
GVATAVATEA PASRKECFGV FCTTYDLKAE DKTKSWRKLV NVAVSGAAGM 100
ISNHLLFKLA SGEVFGQDQP IALKLLGSER SFQALEGVAM ELEDSLYPLL 150
REVSIGIDPY VVFQDVDWAL LIGAKPRGPG MERAALLDIN GQIFADQGKA 200
LNAVASRNDE VLVVGNPCNT NALICLKNAP NIPAKNFHAL TRLDENRAKC 250
QLALKAGVFY DKVSNVTIWG NHSTTQVPDF LNAKIDGRPV KEVIKDTKWL 300
EEEFTLTVQK RGGVLIQKWG RSSAASTAVS IVDAIRSLVT PTPEGDWFST 350
GVYTTGNPYG IAEDIVFSMP CRSKGDGDYE LASDVLMDDF LWERIKKSEA 400
ELLAEKKCVA HLTGEGNAFC DLPEDTMLPG EV 432
Length:432
Mass (Da):46,860
Last modified:April 1, 1990 - v1
Checksum:i45531DEF8BBF79FD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16084 mRNA. Translation: CAA34213.1.
PIRiS04859. DEMZMC.
RefSeqiNP_001105420.1. NM_001111950.1.
UniGeneiZm.122.

Genome annotation databases

GeneIDi542374.
KEGGizma:542374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16084 mRNA. Translation: CAA34213.1 .
PIRi S04859. DEMZMC.
RefSeqi NP_001105420.1. NM_001111950.1.
UniGenei Zm.122.

3D structure databases

ProteinModelPortali P15719.
SMRi P15719. Positions 64-429.
ModBasei Search...

Proteomic databases

PRIDEi P15719.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 542374.
KEGGi zma:542374.

Organism-specific databases

Gramenei P15719.
MaizeGDBi 40092.

Phylogenomic databases

HOGENOMi HOG000220953.
KOi K00051.

Enzyme and pathway databases

BRENDAi 1.1.1.82. 6752.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Maize NADP-malate dehydrogenase: cDNA cloning, sequence, and mRNA characterization."
    Metzler M., Rothermel B.A., Nelson T.
    Plant Mol. Biol. 12:713-722(1989)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.
  2. "Primary structure of the light-dependent regulatory site of corn NADP-malate dehydrogenase."
    Decottignies P., Schmitter J.-M., Miginiac-Maslow M., le Marechal P., Jacquot J.-P., Gadal P.
    J. Biol. Chem. 263:11780-11785(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 58-84.
  3. "Amino acid sequence and molecular weight of native NADP malate dehydrogenase from the C4 plant Zea mays."
    Agostino A., Jeffrey P., Hatch M.D.
    Plant Physiol. 98:1506-1510(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 41-62.

Entry informationi

Entry nameiMDHP_MAIZE
AccessioniPrimary (citable) accession number: P15719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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