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P15719

- MDHP_MAIZE

UniProt

P15719 - MDHP_MAIZE

Protein

Malate dehydrogenase [NADP], chloroplastic

Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

    Catalytic activityi

    (S)-malate + NADP+ = oxaloacetate + NADPH.

    Enzyme regulationi

    Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei67 – 671Activation of NADP-MDH
    Sitei72 – 721Activation of NADP-MDH
    Binding sitei177 – 1771SubstratePROSITE-ProRule annotation
    Binding sitei183 – 1831SubstratePROSITE-ProRule annotation
    Binding sitei190 – 1901NADPBy similarity
    Binding sitei197 – 1971NADBy similarity
    Binding sitei216 – 2161SubstratePROSITE-ProRule annotation
    Binding sitei247 – 2471SubstratePROSITE-ProRule annotation
    Active sitei272 – 2721Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi96 – 1027NADPBy similarity
    Nucleotide bindingi214 – 2163NADPBy similarity

    GO - Molecular functioni

    1. malate dehydrogenase (NADP+) activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BRENDAi1.1.1.82. 6752.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase [NADP], chloroplastic (EC:1.1.1.82)
    Alternative name(s):
    NADP-MDH
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiP15719.
    MaizeGDBi40092.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: EnsemblPlants/Gramene
    2. chloroplast envelope Source: EnsemblPlants/Gramene
    3. chloroplast stroma Source: EnsemblPlants/Gramene
    4. mitochondrion Source: EnsemblPlants/Gramene
    5. thylakoid Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4040Chloroplast1 PublicationAdd
    BLAST
    Chaini41 – 432392Malate dehydrogenase [NADP], chloroplasticPRO_0000018643Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi67 ↔ 72In oxidized inactive NAD-MDH1 Publication
    Disulfide bondi408 ↔ 420In oxidized inactive NAD-MDHBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiP15719.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP15719.
    SMRiP15719. Positions 64-429.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000220953.
    KOiK00051.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011273. Malate_DH_NADP-dep_pln.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
    TIGR01759. MalateDH-SF1. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15719-1 [UniParc]FASTAAdd to Basket

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    MGLSTVYSPA GPRLVPAPLG RCRSAQPRRP RRAPLATVRC SVDATKQAQD    50
    GVATAVATEA PASRKECFGV FCTTYDLKAE DKTKSWRKLV NVAVSGAAGM 100
    ISNHLLFKLA SGEVFGQDQP IALKLLGSER SFQALEGVAM ELEDSLYPLL 150
    REVSIGIDPY VVFQDVDWAL LIGAKPRGPG MERAALLDIN GQIFADQGKA 200
    LNAVASRNDE VLVVGNPCNT NALICLKNAP NIPAKNFHAL TRLDENRAKC 250
    QLALKAGVFY DKVSNVTIWG NHSTTQVPDF LNAKIDGRPV KEVIKDTKWL 300
    EEEFTLTVQK RGGVLIQKWG RSSAASTAVS IVDAIRSLVT PTPEGDWFST 350
    GVYTTGNPYG IAEDIVFSMP CRSKGDGDYE LASDVLMDDF LWERIKKSEA 400
    ELLAEKKCVA HLTGEGNAFC DLPEDTMLPG EV 432
    Length:432
    Mass (Da):46,860
    Last modified:April 1, 1990 - v1
    Checksum:i45531DEF8BBF79FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16084 mRNA. Translation: CAA34213.1.
    PIRiS04859. DEMZMC.
    RefSeqiNP_001105420.1. NM_001111950.1.
    UniGeneiZm.122.

    Genome annotation databases

    GeneIDi542374.
    KEGGizma:542374.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16084 mRNA. Translation: CAA34213.1 .
    PIRi S04859. DEMZMC.
    RefSeqi NP_001105420.1. NM_001111950.1.
    UniGenei Zm.122.

    3D structure databases

    ProteinModelPortali P15719.
    SMRi P15719. Positions 64-429.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P15719.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 542374.
    KEGGi zma:542374.

    Organism-specific databases

    Gramenei P15719.
    MaizeGDBi 40092.

    Phylogenomic databases

    HOGENOMi HOG000220953.
    KOi K00051.

    Enzyme and pathway databases

    BRENDAi 1.1.1.82. 6752.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011273. Malate_DH_NADP-dep_pln.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01757. Malate-DH_plant. 1 hit.
    TIGR01759. MalateDH-SF1. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Maize NADP-malate dehydrogenase: cDNA cloning, sequence, and mRNA characterization."
      Metzler M., Rothermel B.A., Nelson T.
      Plant Mol. Biol. 12:713-722(1989)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Leaf.
    2. "Primary structure of the light-dependent regulatory site of corn NADP-malate dehydrogenase."
      Decottignies P., Schmitter J.-M., Miginiac-Maslow M., le Marechal P., Jacquot J.-P., Gadal P.
      J. Biol. Chem. 263:11780-11785(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 58-84.
    3. "Amino acid sequence and molecular weight of native NADP malate dehydrogenase from the C4 plant Zea mays."
      Agostino A., Jeffrey P., Hatch M.D.
      Plant Physiol. 98:1506-1510(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 41-62.

    Entry informationi

    Entry nameiMDHP_MAIZE
    AccessioniPrimary (citable) accession number: P15719
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3