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Reviewed, UniProtKB/Swiss-Prot P15719 (MDHP_MAIZE)

Last modified June 16, 2009. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Malate dehydrogenase [NADP], chloroplastic
    EC=1.1.1.82
Alternative name(s):
    NADP-MDH
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

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Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

Catalytic activity

(S)-malate + NADP+ = oxaloacetate + NADPH.

Enzyme regulation

Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfides bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

Subunit structure

Homodimer.

Subcellular location

Plastid › chloroplast.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

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Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandNADP
   Molecular functionOxidoreductase
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionbinding

Inferred from electronic annotation. Source: InterPro

malate dehydrogenase (NADP+) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4040Chloroplast Ref.3
Chain41 – 432392Malate dehydrogenase [NADP], chloroplastic
PRO_0000018643

Regions

Nucleotide binding96 – 1027NADP By similarity
Nucleotide binding214 – 2163NADP By similarity

Sites

Active site2721Proton acceptor By similarity
Binding site1771Substrate By similarity
Binding site1831Substrate By similarity
Binding site1901NADP By similarity
Binding site1971NAD By similarity
Binding site2161Substrate By similarity
Binding site2471Substrate By similarity
Site671Activation of NADP-MDH
Site721Activation of NADP-MDH

Amino acid modifications

Disulfide bond67 ↔ 72In oxidized inactive NAD-MDH Ref.2
Disulfide bond408 ↔ 420In oxidized inactive NAD-MDH By similarity

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Sequences

Sequence LengthMass (Da)Tools
P15719-1 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 45531DEF8BBF79FD

FASTA43246,860
        10         20         30         40         50         60 
MGLSTVYSPA GPRLVPAPLG RCRSAQPRRP RRAPLATVRC SVDATKQAQD GVATAVATEA 

        70         80         90        100        110        120 
PASRKECFGV FCTTYDLKAE DKTKSWRKLV NVAVSGAAGM ISNHLLFKLA SGEVFGQDQP 

       130        140        150        160        170        180 
IALKLLGSER SFQALEGVAM ELEDSLYPLL REVSIGIDPY VVFQDVDWAL LIGAKPRGPG 

       190        200        210        220        230        240 
MERAALLDIN GQIFADQGKA LNAVASRNDE VLVVGNPCNT NALICLKNAP NIPAKNFHAL 

       250        260        270        280        290        300 
TRLDENRAKC QLALKAGVFY DKVSNVTIWG NHSTTQVPDF LNAKIDGRPV KEVIKDTKWL 

       310        320        330        340        350        360 
EEEFTLTVQK RGGVLIQKWG RSSAASTAVS IVDAIRSLVT PTPEGDWFST GVYTTGNPYG 

       370        380        390        400        410        420 
IAEDIVFSMP CRSKGDGDYE LASDVLMDDF LWERIKKSEA ELLAEKKCVA HLTGEGNAFC 

       430 
DLPEDTMLPG EV

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References

[1]"Maize NADP-malate dehydrogenase: cDNA cloning, sequence, and mRNA characterization."
Metzler M., Rothermel B.A., Nelson T.
Plant Mol. Biol. 12:713-722(1989)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leaf.
[2]"Primary structure of the light-dependent regulatory site of corn NADP-malate dehydrogenase."
Decottignies P., Schmitter J.-M., Miginiac-Maslow M., le Marechal P., Jacquot J.-P., Gadal P.
J. Biol. Chem. 263:11780-11785(1988) [PubMed: 3403553] [Abstract]
Cited for: PROTEIN SEQUENCE OF 58-84.
[3]"Amino acid sequence and molecular weight of native NADP malate dehydrogenase from the C4 plant Zea mays."
Agostino A., Jeffrey P., Hatch M.D.
Plant Physiol. 98:1506-1510(1992) [PubMed: 16668822] [Abstract]
Cited for: PROTEIN SEQUENCE OF 41-62.

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Cross-references

Sequence databases

X16084 mRNA. Translation: CAA34213.1.
PIRDEMZMC. S04859.
RefSeqNP_001105420.1.
UniGeneZm.122

3D structure databases

HSSPHSSP built from PDB template 7MDH based on UniProtKB P17606.
SMRP15719. Positions 64-429.
ModBaseSearch...

Genome annotation databases

GeneID542374.

Organism-specific databases

GrameneP15719.
MaizeGDB40092.

Enzyme and pathway databases

BRENDA1.1.1.82. 289.

Family and domain databases

InterProIPR001236. Lactate/malate_DH.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_SF1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR23382. MDH_SF1. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
ProDomPD003052. Mal_dehydrog. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMDHP_MAIZE
AccessionPrimary (citable) accession number: P15719
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents