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Protein

Malate dehydrogenase [NADP], chloroplastic

Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

Catalytic activityi

(S)-malate + NADP+ = oxaloacetate + NADPH.

Enzyme regulationi

Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei67Activation of NADP-MDH1
Sitei72Activation of NADP-MDH1
Binding sitei177SubstratePROSITE-ProRule annotation1
Binding sitei183SubstratePROSITE-ProRule annotation1
Binding sitei190NADPBy similarity1
Binding sitei197NADBy similarity1
Binding sitei216SubstratePROSITE-ProRule annotation1
Binding sitei247SubstratePROSITE-ProRule annotation1
Active sitei272Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi96 – 102NADPBy similarity7
Nucleotide bindingi214 – 216NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.82. 6752.
SABIO-RKP15719.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase [NADP], chloroplastic (EC:1.1.1.82)
Alternative name(s):
NADP-MDH
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Organism-specific databases

MaizeGDBi40092.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 40Chloroplast1 PublicationAdd BLAST40
ChainiPRO_000001864341 – 432Malate dehydrogenase [NADP], chloroplasticAdd BLAST392

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi67 ↔ 72In oxidized inactive NAD-MDH1 Publication
Disulfide bondi408 ↔ 420In oxidized inactive NAD-MDHBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP15719.
PRIDEiP15719.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi4577.GRMZM2G129513_P01.

Structurei

3D structure databases

ProteinModelPortaliP15719.
SMRiP15719.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1496. Eukaryota.
COG0039. LUCA.
HOGENOMiHOG000220953.
KOiK00051.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15719-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSTVYSPA GPRLVPAPLG RCRSAQPRRP RRAPLATVRC SVDATKQAQD
60 70 80 90 100
GVATAVATEA PASRKECFGV FCTTYDLKAE DKTKSWRKLV NVAVSGAAGM
110 120 130 140 150
ISNHLLFKLA SGEVFGQDQP IALKLLGSER SFQALEGVAM ELEDSLYPLL
160 170 180 190 200
REVSIGIDPY VVFQDVDWAL LIGAKPRGPG MERAALLDIN GQIFADQGKA
210 220 230 240 250
LNAVASRNDE VLVVGNPCNT NALICLKNAP NIPAKNFHAL TRLDENRAKC
260 270 280 290 300
QLALKAGVFY DKVSNVTIWG NHSTTQVPDF LNAKIDGRPV KEVIKDTKWL
310 320 330 340 350
EEEFTLTVQK RGGVLIQKWG RSSAASTAVS IVDAIRSLVT PTPEGDWFST
360 370 380 390 400
GVYTTGNPYG IAEDIVFSMP CRSKGDGDYE LASDVLMDDF LWERIKKSEA
410 420 430
ELLAEKKCVA HLTGEGNAFC DLPEDTMLPG EV
Length:432
Mass (Da):46,860
Last modified:April 1, 1990 - v1
Checksum:i45531DEF8BBF79FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16084 mRNA. Translation: CAA34213.1.
PIRiS04859. DEMZMC.
RefSeqiNP_001105420.1. NM_001111950.1.
UniGeneiZm.122.

Genome annotation databases

GeneIDi542374.
KEGGizma:542374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16084 mRNA. Translation: CAA34213.1.
PIRiS04859. DEMZMC.
RefSeqiNP_001105420.1. NM_001111950.1.
UniGeneiZm.122.

3D structure databases

ProteinModelPortaliP15719.
SMRiP15719.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G129513_P01.

Proteomic databases

PaxDbiP15719.
PRIDEiP15719.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi542374.
KEGGizma:542374.

Organism-specific databases

MaizeGDBi40092.

Phylogenomic databases

eggNOGiKOG1496. Eukaryota.
COG0039. LUCA.
HOGENOMiHOG000220953.
KOiK00051.

Enzyme and pathway databases

BRENDAi1.1.1.82. 6752.
SABIO-RKP15719.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDHP_MAIZE
AccessioniPrimary (citable) accession number: P15719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.