P15719 (MDHP_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Malate dehydrogenase [NADP], chloroplastic EC=1.1.1.82 Alternative name(s): NADP-MDH
Organism	Zea mays (Maize)
Taxonomic identifier	4577 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.
Catalytic activity	(S)-malate + NADP+ = oxaloacetate + NADPH.
Enzyme regulation	Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.
Subunit structure	Homodimer.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast envelope Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast stroma Inferred from electronic annotation. Source: EnsemblPlants/Gramene mitochondrion Inferred from electronic annotation. Source: EnsemblPlants/Gramene thylakoid Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	malate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Chloroplast Ref.3
	Chain	41 – 432	392	Malate dehydrogenase [NADP], chloroplastic		PRO_0000018643
Regions
	Nucleotide binding	96 – 102	7	NADP By similarity
	Nucleotide binding	214 – 216	3	NADP By similarity
Sites
	Active site	272	1	Proton acceptor By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
	Binding site	190	1	NADP By similarity
	Binding site	197	1	NAD By similarity
	Binding site	216	1	Substrate By similarity
	Binding site	247	1	Substrate By similarity
	Site	67	1	Activation of NADP-MDH
	Site	72	1	Activation of NADP-MDH
Amino acid modifications
	Disulfide bond	67 ↔ 72		In oxidized inactive NAD-MDH Ref.2
	Disulfide bond	408 ↔ 420		In oxidized inactive NAD-MDH By similarity

Sequences

	Sequence		Length	Mass (Da)
	P15719 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 45531DEF8BBF79FD	FASTA	432	46,860
	10 20 30 40 50 60 MGLSTVYSPA GPRLVPAPLG RCRSAQPRRP RRAPLATVRC SVDATKQAQD GVATAVATEA 70 80 90 100 110 120 PASRKECFGV FCTTYDLKAE DKTKSWRKLV NVAVSGAAGM ISNHLLFKLA SGEVFGQDQP 130 140 150 160 170 180 IALKLLGSER SFQALEGVAM ELEDSLYPLL REVSIGIDPY VVFQDVDWAL LIGAKPRGPG 190 200 210 220 230 240 MERAALLDIN GQIFADQGKA LNAVASRNDE VLVVGNPCNT NALICLKNAP NIPAKNFHAL 250 260 270 280 290 300 TRLDENRAKC QLALKAGVFY DKVSNVTIWG NHSTTQVPDF LNAKIDGRPV KEVIKDTKWL 310 320 330 340 350 360 EEEFTLTVQK RGGVLIQKWG RSSAASTAVS IVDAIRSLVT PTPEGDWFST GVYTTGNPYG 370 380 390 400 410 420 IAEDIVFSMP CRSKGDGDYE LASDVLMDDF LWERIKKSEA ELLAEKKCVA HLTGEGNAFC 430 DLPEDTMLPG EV

References

[1]	"Maize NADP-malate dehydrogenase: cDNA cloning, sequence, and mRNA characterization." Metzler M., Rothermel B.A., Nelson T. Plant Mol. Biol. 12:713-722(1989) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[2]	"Primary structure of the light-dependent regulatory site of corn NADP-malate dehydrogenase." Decottignies P., Schmitter J.-M., Miginiac-Maslow M., le Marechal P., Jacquot J.-P., Gadal P. J. Biol. Chem. 263:11780-11785(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 58-84.
[3]	"Amino acid sequence and molecular weight of native NADP malate dehydrogenase from the C4 plant Zea mays." Agostino A., Jeffrey P., Hatch M.D. Plant Physiol. 98:1506-1510(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 41-62.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16084 mRNA. Translation: CAA34213.1.
PIR	DEMZMC. S04859.
RefSeq	NP_001105420.1. NM_001111950.1.
UniGene	Zm.122.
3D structure databases
ProteinModelPortal	P15719.
SMR	P15719. Positions 64-429.
ModBase	Search...
Proteomic databases
PRIDE	P15719.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	542374.
KEGG	zma:542374.
Organism-specific databases
Gramene	P15719.
MaizeGDB	40092.
Phylogenomic databases
HOGENOM	HOG000220953.
KO	K00051.
Enzyme and pathway databases
BRENDA	1.1.1.82. 6752.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
InterPro	IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR011273. Malate_DH_NADP-dep_pln. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR23382. PTHR23382. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01757. Malate-DH_plant. 1 hit. TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHP_MAIZE

Accession

Primary (citable) accession number: P15719

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program