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Protein

Bile salt sulfotransferase

Gene

St2a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze sulfonation of hydroxysteroids and xenobiotics. Bioactivation of carcinogenic polycyclic arylmethanols.

Catalytic activityi

3'-phosphoadenylyl sulfate + glycolithocholate = adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate.
3'-phosphoadenylyl sulfate + taurolithocholate = adenosine 3',5'-bisphosphate + taurolithocholate sulfate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711SubstrateBy similarity
Binding sitei76 – 761SubstrateBy similarity
Active sitei98 – 981Proton acceptorBy similarity
Binding sitei120 – 1201PAPSBy similarity
Binding sitei128 – 1281PAPSBy similarity
Binding sitei183 – 1831PAPSBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 486PAPSBy similarity
Nucleotide bindingi217 – 2226PAPSBy similarity
Nucleotide bindingi246 – 2483PAPSBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Bile salt sulfotransferase (EC:2.8.2.14)
Alternative name(s):
Hydroxysteroid sulfotransferase
Short name:
ST
ST-20
Sulfotransferase 2A1
Short name:
ST2A1
Gene namesi
Name:St2a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3007.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 284283Bile salt sulfotransferasePRO_0000085144Add
BLAST

Proteomic databases

PaxDbiP15709.
PRIDEiP15709.

PTM databases

iPTMnetiP15709.
PhosphoSiteiP15709.

Expressioni

Developmental stagei

There is a marked sex difference (female >> male) in the expressed level of mRNA for this protein.

Inductioni

Induced by estrogens and suppressed by androgens. Expression is under the influence of pituitary growth hormone and thyroid hormone.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043445.

Chemistry

BindingDBiP15709.

Structurei

3D structure databases

ProteinModelPortaliP15709.
SMRiP15709. Positions 3-284.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Phylogenomic databases

eggNOGiKOG1584. Eukaryota.
ENOG4111H56. LUCA.
HOGENOMiHOG000037209.
HOVERGENiHBG001195.
InParanoidiP15709.
KOiK11822.
PhylomeDBiP15709.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15709-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDYTWFEGI PFHAFGISKE TLQNVCNKFV VKEEDLILLA YPKSGTNWLI
60 70 80 90 100
EIVCLIQTKG DPKWIQSVTI WDRSPWIETD VGYDILIKKK GPRLMTSHLP
110 120 130 140 150
MHLFSKSLFS SKAKVIYLIR NPRDVLVSGY YFWGNSTLVK KPDSLGTYVE
160 170 180 190 200
WFLKGNVLYG SWFEHIRAWL SMREWDNFLL LYYEDMKKDT MGTIKKICDF
210 220 230 240 250
LGKKLEPDEL DLVLKYSSFQ VMKENDMSNY SLLMKKSIFT GIGLMRKGTI
260 270 280
GDWKNHFTVS QAEAFDKVFQ EKMAGFPPGM FPWE
Length:284
Mass (Da):33,191
Last modified:January 23, 2007 - v3
Checksum:i07A82B2A96CD4AF5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331E → D in AAA41356 (PubMed:2590219).Curated
Sequence conflicti119 – 1191I → V in AAA41356 (PubMed:2590219).Curated
Sequence conflicti139 – 1391V → A in AAA41356 (PubMed:2590219).Curated
Sequence conflicti173 – 1731R → Q in AAA41356 (PubMed:2590219).Curated
Sequence conflicti242 – 2421I → T in AAA41356 (PubMed:2590219).Curated
Sequence conflicti250 – 2501I → V in AAA41356 (PubMed:2590219).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31363 mRNA. Translation: AAA41356.1.
D14988 mRNA. Translation: BAA03633.1.
D14987 mRNA. Translation: BAA03632.1.
PIRiA33569.
I52849.
RefSeqiNP_571978.1. NM_131903.1.
UniGeneiRn.91378.

Genome annotation databases

GeneIDi24912.
KEGGirno:24912.
UCSCiRGD:621337. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31363 mRNA. Translation: AAA41356.1.
D14988 mRNA. Translation: BAA03633.1.
D14987 mRNA. Translation: BAA03632.1.
PIRiA33569.
I52849.
RefSeqiNP_571978.1. NM_131903.1.
UniGeneiRn.91378.

3D structure databases

ProteinModelPortaliP15709.
SMRiP15709. Positions 3-284.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043445.

Chemistry

BindingDBiP15709.
ChEMBLiCHEMBL3007.

PTM databases

iPTMnetiP15709.
PhosphoSiteiP15709.

Proteomic databases

PaxDbiP15709.
PRIDEiP15709.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24912.
KEGGirno:24912.
UCSCiRGD:621337. rat.

Organism-specific databases

CTDi6822.

Phylogenomic databases

eggNOGiKOG1584. Eukaryota.
ENOG4111H56. LUCA.
HOGENOMiHOG000037209.
HOVERGENiHBG001195.
InParanoidiP15709.
KOiK11822.
PhylomeDBiP15709.

Miscellaneous databases

PROiP15709.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence analysis of a rat liver cDNA encoding hydroxysteroid sulfotransferase."
    Ogura K., Kajita J., Narihata H., Watabe T., Ozawa S., Nagata K., Yamazoe Y., Kato R.
    Biochem. Biophys. Res. Commun. 165:168-174(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Molecular cloning and functions of rat liver hydroxysteroid sulfotransferases catalysing covalent binding of carcinogenic polycyclic arylmethanols to DNA."
    Watabe T., Ogura K., Satsukawa M., Okuda H., Hiratsuka A.
    Chem. Biol. Interact. 92:87-105(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.

Entry informationi

Entry nameiST2A1_RAT
AccessioniPrimary (citable) accession number: P15709
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.