P15705 (STI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heat shock protein STI1

Gene names

Name:	STI1
Ordered Locus Names:	YOR027W
ORF Names:	OR26.17

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in mediating the heat shock response of some HSP70 genes. It is required for optimal growth of yeast cells at both low and high temperature.
Subunit structure	Part of a larger complex that includes HSP70, HSP90, and immunophilins.
Subcellular location	Cytoplasm Ref.8.
Induction	By heat shock and canavanine.
Post-translational modification	N-glycosylated. Ref.12
Miscellaneous	Present with 67600 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Contains 2 STI1 domains. Contains 9 TPR repeats.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Domain	Repeat TPR repeat
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from mutant phenotype. Source: SGD response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from physical interaction. Source: SGD
Molecular function	ATPase inhibitor activity Inferred from direct assay. Source: SGD Hsp70 protein binding Inferred from direct assay. Source: SGD Hsp90 protein binding Inferred from direct assay. Source: SGD mRNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HSC82	P15108	3	EBI-18418,EBI-8666
HSP82	P02829	8	EBI-18418,EBI-8659

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Heat shock protein STI1		PRO_0000106341
Regions
	Repeat	5 – 38	34	TPR 1
	Repeat	40 – 73	34	TPR 2
	Repeat	74 – 107	34	TPR 3
	Domain	138 – 177	40	STI1 1
	Repeat	262 – 295	34	TPR 4
	Repeat	297 – 328	32	TPR 5
	Repeat	336 – 369	34	TPR 6
	Repeat	396 – 429	34	TPR 7
	Repeat	430 – 463	34	TPR 8
	Repeat	465 – 492	28	TPR 9
	Domain	537 – 576	40	STI1 2
Amino acid modifications
	Modified residue	2	1	Phosphoserine Ref.11
	Modified residue	227	1	Phosphoserine Ref.10 Ref.11
	Modified residue	523	1	Phosphoserine Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P15705 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: CF8A85C2BBD3C44C	FASTA	589	66,265
	10 20 30 40 50 60 MSLTADEYKQ QGNAAFTAKD YDKAIELFTK AIEVSETPNH VLYSNRSACY TSLKKFSDAL 70 80 90 100 110 120 NDANECVKIN PSWSKGYNRL GAAHLGLGDL DEAESNYKKA LELDASNKAA KEGLDQVHRT 130 140 150 160 170 180 QQARQAQPDL GLTQLFADPN LIENLKKNPK TSEMMKDPQL VAKLIGYKQN PQAIGQDLFT 190 200 210 220 230 240 DPRLMTIMAT LMGVDLNMDD INQSNSMPKE PETSKSTEQK KDAEPQSDST TSKENSSKAP 250 260 270 280 290 300 QKEESKESEP MEVDEDDSKI EADKEKAEGN KFYKARQFDE AIEHYNKAWE LHKDITYLNN 310 320 330 340 350 360 RAAAEYEKGE YETAISTLND AVEQGREMRA DYKVISKSFA RIGNAYHKLG DLKKTIEYYQ 370 380 390 400 410 420 KSLTEHRTAD ILTKLRNAEK ELKKAEAEAY VNPEKAEEAR LEGKEYFTKS DWPNAVKAYT 430 440 450 460 470 480 EMIKRAPEDA RGYSNRAAAL AKLMSFPEAI ADCNKAIEKD PNFVRAYIRK ATAQIAVKEY 490 500 510 520 530 540 ASALETLDAA RTKDAEVNNG SSAREIDQLY YKASQQRFQP GTSNETPEET YQRAMKDPEV 550 560 570 580 AAIMQDPVMQ SILQQAQQNP AALQEHMKNP EVFKKIQTLI AAGIIRTGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of STI1, a stress-inducible gene from Saccharomyces cerevisiae." Nicolet C.M., Craig E.A. Mol. Cell. Biol. 9:3638-3646(1989) [PubMed: 2674681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Snap helix with knob and hole: essential repeats in S. pombe nuclear protein nuc2+." Hirano T., Kinoshita N., Morikawa K., Yanagida M. Cell 60:319-328(1990) [PubMed: 2297790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Protein identifications for a Saccharomyces cerevisiae protein database." Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S. Electrophoresis 15:1466-1486(1994) [PubMed: 7895733] [Abstract] Cited for: PROTEIN SEQUENCE OF 76-93. Strain: ATCC 204508 / S288c.
[6]	"Expanding family." Boguski M.S., Sikorski R.S., Hieter P.A., Goebl M. Nature 346:114-114(1990) [PubMed: 1694969] [Abstract] Cited for: DOMAINS TPR REPEATS.
[7]	"Identification of a 60-kilodalton stress-related protein, p60, which interacts with hsp90 and hsp70." Smith D.F., Sullivan W.P., Marion T.N., Zaitsu K., Madden B., McCormick D.J., Toft D.O. Mol. Cell. Biol. 13:869-876(1993) [PubMed: 8423808] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-227 AND SER-523, MASS SPECTROMETRY.
[12]	"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes." Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H. Mol. Syst. Biol. 5:308-308(2009) [PubMed: 19756047] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28486 Genomic DNA. Translation: AAA35121.1. X87331 Genomic DNA. Translation: CAA60743.1. Z74935 Genomic DNA. Translation: CAA99217.1. BK006948 Genomic DNA. Translation: DAA10809.1.
PIR	A32567.
RefSeq	NP_014670.1. NM_001183446.1.
3D structure databases
ProteinModelPortal	P15705.
SMR	P15705. Positions 4-123, 261-507.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2329N.
IntAct	P15705. 37 interactions.
MINT	MINT-707004.
STRING	P15705.
2D gel databases
SWISS-2DPAGE	P15705.
Proteomic databases
PeptideAtlas	P15705.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR027W; YOR027W; YOR027W.
GeneID	854192.
KEGG	sce:YOR027W.
NMPDR	fig|4932.3.peg.5768.
Organism-specific databases
CYGD	YOR027w.
SGD	S000005553. STI1.
Phylogenomic databases
eggNOG	fuNOG04387.
GeneTree	EFGT00050000001948.
HOGENOM	HBG384096.
OMA	RSACYAS.
OrthoDB	EOG4XD70P.
Gene expression databases
ArrayExpress	P15705.
Genevestigator	P15705.
GermOnline	YOR027W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006636. STI1_HS-bd. IPR001440. TPR-1. IPR013026. TPR-contain. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 3 hits.
KO	K09553.
Pfam	PF00515. TPR_1. 4 hits. [Graphical view]
SMART	SM00727. STI1. 2 hits. SM00028. TPR. 9 hits. [Graphical view]
PROSITE	PS50005. TPR. 7 hits. PS50293. TPR_REGION. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	976014.

Entry information

Entry name

STI1_YEAST

Accession

Primary (citable) accession number: P15705
Secondary accession number(s): D6W293

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	December 14, 2011
This is version 125 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program