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Protein

Heat shock protein STI1

Gene

STI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in mediating the heat shock response of some HSP70 genes. It is required for optimal growth of yeast cells at both low and high temperature.

GO - Molecular functioni

  • ATPase inhibitor activity Source: SGD
  • Hsp70 protein binding Source: SGD
  • Hsp90 protein binding Source: SGD
  • mRNA binding Source: SGD

GO - Biological processi

  • protein folding Source: SGD
  • protein localization Source: SGD
  • protein targeting to mitochondrion Source: SGD
Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-33574-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein STI1
Gene namesi
Name:STI1
Ordered Locus Names:YOR027W
ORF Names:OR26.17
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR027W.
SGDiS000005553. STI1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063411 – 589Heat shock protein STI1Add BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki168Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei227PhosphoserineCombined sources1
Cross-linki384Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP15705.
PRIDEiP15705.

2D gel databases

SWISS-2DPAGEP15705.

PTM databases

iPTMnetiP15705.

Expressioni

Inductioni

By heat shock and canavanine.

Interactioni

Subunit structurei

Part of a larger complex that includes HSP70, HSP90, and immunophilins.

Binary interactionsi

WithEntry#Exp.IntActNotes
HSC82P151083EBI-18418,EBI-8666
HSP82P028299EBI-18418,EBI-8659

GO - Molecular functioni

  • Hsp70 protein binding Source: SGD
  • Hsp90 protein binding Source: SGD

Protein-protein interaction databases

BioGridi34430. 105 interactors.
DIPiDIP-2329N.
IntActiP15705. 17 interactors.
MINTiMINT-707004.

Structurei

Secondary structure

1589
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi133 – 137Combined sources5
Beta strandi138 – 140Combined sources3
Helixi142 – 147Combined sources6
Helixi152 – 156Combined sources5
Helixi160 – 169Combined sources10
Helixi174 – 177Combined sources4
Turni178 – 180Combined sources3
Helixi182 – 192Combined sources11
Turni193 – 196Combined sources4
Helixi262 – 274Combined sources13
Helixi278 – 291Combined sources14
Helixi296 – 307Combined sources12
Helixi311 – 327Combined sources17
Helixi332 – 348Combined sources17
Helixi352 – 365Combined sources14
Helixi369 – 390Combined sources22
Helixi395 – 408Combined sources14
Helixi412 – 425Combined sources14
Helixi430 – 442Combined sources13
Helixi446 – 459Combined sources14
Helixi464 – 476Combined sources13
Helixi480 – 498Combined sources19
Turni499 – 502Combined sources4
Helixi503 – 516Combined sources14
Helixi527 – 536Combined sources10
Helixi538 – 544Combined sources7
Helixi548 – 558Combined sources11
Helixi560 – 568Combined sources9
Helixi570 – 581Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LLVNMR-A127-197[»]
2LLWNMR-A519-589[»]
3UPVX-ray1.60A395-518[»]
3UQ3X-ray2.60A262-515[»]
ProteinModelPortaliP15705.
SMRiP15705.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati5 – 38TPR 1Add BLAST34
Repeati40 – 73TPR 2Add BLAST34
Repeati74 – 107TPR 3Add BLAST34
Domaini138 – 177STI1 1Add BLAST40
Repeati262 – 295TPR 4Add BLAST34
Repeati297 – 328TPR 5Add BLAST32
Repeati336 – 369TPR 6Add BLAST34
Repeati396 – 429TPR 7Add BLAST34
Repeati430 – 463TPR 8Add BLAST34
Repeati465 – 492TPR 9Add BLAST28
Domaini537 – 576STI1 2Add BLAST40

Sequence similaritiesi

Contains 2 STI1 domains.Curated
Contains 9 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

GeneTreeiENSGT00840000129761.
HOGENOMiHOG000186562.
InParanoidiP15705.
KOiK09553.
OMAiSWSKGYN.
OrthoDBiEOG092C1UGR.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR006636. STI1_HS-bd.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 2 hits.
PF13181. TPR_8. 2 hits.
[Graphical view]
SMARTiSM00727. STI1. 2 hits.
SM00028. TPR. 9 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 7 hits.
PS50293. TPR_REGION. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLTADEYKQ QGNAAFTAKD YDKAIELFTK AIEVSETPNH VLYSNRSACY
60 70 80 90 100
TSLKKFSDAL NDANECVKIN PSWSKGYNRL GAAHLGLGDL DEAESNYKKA
110 120 130 140 150
LELDASNKAA KEGLDQVHRT QQARQAQPDL GLTQLFADPN LIENLKKNPK
160 170 180 190 200
TSEMMKDPQL VAKLIGYKQN PQAIGQDLFT DPRLMTIMAT LMGVDLNMDD
210 220 230 240 250
INQSNSMPKE PETSKSTEQK KDAEPQSDST TSKENSSKAP QKEESKESEP
260 270 280 290 300
MEVDEDDSKI EADKEKAEGN KFYKARQFDE AIEHYNKAWE LHKDITYLNN
310 320 330 340 350
RAAAEYEKGE YETAISTLND AVEQGREMRA DYKVISKSFA RIGNAYHKLG
360 370 380 390 400
DLKKTIEYYQ KSLTEHRTAD ILTKLRNAEK ELKKAEAEAY VNPEKAEEAR
410 420 430 440 450
LEGKEYFTKS DWPNAVKAYT EMIKRAPEDA RGYSNRAAAL AKLMSFPEAI
460 470 480 490 500
ADCNKAIEKD PNFVRAYIRK ATAQIAVKEY ASALETLDAA RTKDAEVNNG
510 520 530 540 550
SSAREIDQLY YKASQQRFQP GTSNETPEET YQRAMKDPEV AAIMQDPVMQ
560 570 580
SILQQAQQNP AALQEHMKNP EVFKKIQTLI AAGIIRTGR
Length:589
Mass (Da):66,265
Last modified:April 1, 1990 - v1
Checksum:iCF8A85C2BBD3C44C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28486 Genomic DNA. Translation: AAA35121.1.
X87331 Genomic DNA. Translation: CAA60743.1.
Z74935 Genomic DNA. Translation: CAA99217.1.
BK006948 Genomic DNA. Translation: DAA10809.1.
PIRiA32567.
RefSeqiNP_014670.1. NM_001183446.1.

Genome annotation databases

EnsemblFungiiYOR027W; YOR027W; YOR027W.
GeneIDi854192.
KEGGisce:YOR027W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28486 Genomic DNA. Translation: AAA35121.1.
X87331 Genomic DNA. Translation: CAA60743.1.
Z74935 Genomic DNA. Translation: CAA99217.1.
BK006948 Genomic DNA. Translation: DAA10809.1.
PIRiA32567.
RefSeqiNP_014670.1. NM_001183446.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LLVNMR-A127-197[»]
2LLWNMR-A519-589[»]
3UPVX-ray1.60A395-518[»]
3UQ3X-ray2.60A262-515[»]
ProteinModelPortaliP15705.
SMRiP15705.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34430. 105 interactors.
DIPiDIP-2329N.
IntActiP15705. 17 interactors.
MINTiMINT-707004.

PTM databases

iPTMnetiP15705.

2D gel databases

SWISS-2DPAGEP15705.

Proteomic databases

MaxQBiP15705.
PRIDEiP15705.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR027W; YOR027W; YOR027W.
GeneIDi854192.
KEGGisce:YOR027W.

Organism-specific databases

EuPathDBiFungiDB:YOR027W.
SGDiS000005553. STI1.

Phylogenomic databases

GeneTreeiENSGT00840000129761.
HOGENOMiHOG000186562.
InParanoidiP15705.
KOiK09553.
OMAiSWSKGYN.
OrthoDBiEOG092C1UGR.

Enzyme and pathway databases

BioCyciYEAST:G3O-33574-MONOMER.

Miscellaneous databases

PROiP15705.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR006636. STI1_HS-bd.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 2 hits.
PF13181. TPR_8. 2 hits.
[Graphical view]
SMARTiSM00727. STI1. 2 hits.
SM00028. TPR. 9 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 7 hits.
PS50293. TPR_REGION. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTI1_YEAST
AccessioniPrimary (citable) accession number: P15705
Secondary accession number(s): D6W293
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 174 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 67600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.