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P15704

- GUN_CLOSA

UniProt

P15704 - GUN_CLOSA

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Protein

Endoglucanase

Gene

eglA

Organism
Clostridium saccharobutylicum
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei175 – 1751Proton donorBy similarity
Active sitei263 – 2631NucleophileBy similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:eglA
OrganismiClostridium saccharobutylicum
Taxonomic identifieri169679 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Add
BLAST
Chaini35 – 448414EndoglucanasePRO_0000007844Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP15704.
SMRiP15704. Positions 45-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR004867. CHB_HEX_C_dom.
IPR026876. Fn3_assoc_repeat.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
PF03174. CHB_HEX_C. 1 hit.
PF13287. Fn3_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15704-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFSKIKKINF FKKTFSFLIA VVMMLFTVLG TNTYKAEAAT TSFGGQLKVV
60 70 80 90 100
GSQLCDSNGK PIQLKGMSSH GLQWYVNFVN YDSMKFLRDK WGVNVIRAAM
110 120 130 140 150
YTNEGGYISN PSSQKEKIKK IVQDAIDLNM YVIIDWHILS DNNPNTYKEQ
160 170 180 190 200
AKSFFQEMAE EYGKYSNVIY EICNEPNGGT NWANDIKPYA NYIIPAIRAI
210 220 230 240 250
DPNNIIIVGT STWSQDVDIA ADNPLRYSNI MYTCHFYAGT HTQSLRDKIN
260 270 280 290 300
YAMSKGIAIF VTEWGTSDAS GNGGPYLDES QKWVDFMASK NISWTNWALC
310 320 330 340 350
DKSEASAALK SGSSTTGGWT DSDLTTSGLF VKKSIGGSNT TSQTSAPTFS
360 370 380 390 400
LQSGTYDSAQ TVTLTSSDND SVIHYTTDGT TPTSSSPVYT SPITISKTTT
410 420 430 440
VKAFTTKTGM TDSNITSAVY TISNTDPVKQ VSAPTFSYNQ EHTIQLKL
Length:448
Mass (Da):49,366
Last modified:April 1, 1990 - v1
Checksum:i6FE6AC97C836A598
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31311 Genomic DNA. Translation: AAA23230.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31311 Genomic DNA. Translation: AAA23230.1 .

3D structure databases

ProteinModelPortali P15704.
SMRi P15704. Positions 45-335.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR004867. CHB_HEX_C_dom.
IPR026876. Fn3_assoc_repeat.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
PF03174. CHB_HEX_C. 1 hit.
PF13287. Fn3_assoc. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure of an endo-beta-1,4-glucanase gene from Clostridium acetobutylicum P262 showing homology with endoglucanase genes from Bacillus spp."
    Zappe H., Jones W.A., Jones D.T., Woods D.R.
    Appl. Environ. Microbiol. 54:1289-1292(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-117 / DSM 13864 / NCP 262.

Entry informationi

Entry nameiGUN_CLOSA
AccessioniPrimary (citable) accession number: P15704
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 1, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The C-terminal region of C.acetobutylicum is not required for activity.

Caution

Was originally thought to originate from C.acetobutylicum.1 Publication

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3