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P15704 (GUN_CLOSA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase

EC=3.2.1.4
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Gene names
Name:eglA
OrganismClostridium saccharobutylicum
Taxonomic identifier169679 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Miscellaneous

The C-terminal region of C.acetobutylicum is not required for activity.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Caution

Was originally (Ref.1) thought to originate from C.acetobutylicum.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434
Chain35 – 448414Endoglucanase
PRO_0000007844

Sites

Active site1751Proton donor By similarity
Active site2631Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P15704 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 6FE6AC97C836A598

FASTA44849,366
        10         20         30         40         50         60 
MFSKIKKINF FKKTFSFLIA VVMMLFTVLG TNTYKAEAAT TSFGGQLKVV GSQLCDSNGK 

        70         80         90        100        110        120 
PIQLKGMSSH GLQWYVNFVN YDSMKFLRDK WGVNVIRAAM YTNEGGYISN PSSQKEKIKK 

       130        140        150        160        170        180 
IVQDAIDLNM YVIIDWHILS DNNPNTYKEQ AKSFFQEMAE EYGKYSNVIY EICNEPNGGT 

       190        200        210        220        230        240 
NWANDIKPYA NYIIPAIRAI DPNNIIIVGT STWSQDVDIA ADNPLRYSNI MYTCHFYAGT 

       250        260        270        280        290        300 
HTQSLRDKIN YAMSKGIAIF VTEWGTSDAS GNGGPYLDES QKWVDFMASK NISWTNWALC 

       310        320        330        340        350        360 
DKSEASAALK SGSSTTGGWT DSDLTTSGLF VKKSIGGSNT TSQTSAPTFS LQSGTYDSAQ 

       370        380        390        400        410        420 
TVTLTSSDND SVIHYTTDGT TPTSSSPVYT SPITISKTTT VKAFTTKTGM TDSNITSAVY 

       430        440 
TISNTDPVKQ VSAPTFSYNQ EHTIQLKL 

« Hide

References

[1]"Structure of an endo-beta-1,4-glucanase gene from Clostridium acetobutylicum P262 showing homology with endoglucanase genes from Bacillus spp."
Zappe H., Jones W.A., Jones D.T., Woods D.R.
Appl. Environ. Microbiol. 54:1289-1292(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC BAA-117 / DSM 13864 / NCP 262.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31311 Genomic DNA. Translation: AAA23230.1.

3D structure databases

ProteinModelPortalP15704.
SMRP15704. Positions 45-335.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR004867. CHB_HEX_C_dom.
IPR026876. Fn3_assoc_repeat.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
PF03174. CHB_HEX_C. 1 hit.
PF13287. Fn3_assoc. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN_CLOSA
AccessionPrimary (citable) accession number: P15704
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 16, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries