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P15703 (BGL2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan 1,3-beta-glucosidase

EC=3.2.1.58
Alternative name(s):
Exo-1,3-beta-glucanase
GP29
Soluble cell wall protein 9
Gene names
Name:BGL2
Synonyms:SCW9
Ordered Locus Names:YGR282C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glucanases possibly play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase.

Catalytic activity

Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.

Subcellular location

Secretedcell wall. Note: Tightly bound to cell wall. Ref.6

Miscellaneous

This protein strongly binds to glucan and chitin.

Present with 45000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.6 Ref.7
Chain24 – 313290Glucan 1,3-beta-glucosidase
PRO_0000011896

Sites

Active site2331Nucleophile By similarity
Active site2921Proton donor By similarity

Amino acid modifications

Glycosylation2021N-linked (GlcNAc...) Potential
Glycosylation2841N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P15703 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 118D75528C5AD383

FASTA31334,119
        10         20         30         40         50         60 
MRFSTTLATA ATALFFTASQ VSAIGELAFN LGVKNNDGTC KSTSDYETEL QALKSYTSTV 

        70         80         90        100        110        120 
KVYAASDCNT LQNLGPAAEA EGFTIFVGVW PTDDSHYAAE KAALQTYLPK IKESTVAGFL 

       130        140        150        160        170        180 
VGSEALYRND LTASQLSDKI NDVRSVVADI SDSDGKSYSG KQVGTVDSWN VLVAGYNSAV 

       190        200        210        220        230        240 
IEASDFVMAN AFSYWQGQTM QNASYSFFDD IMQALQVIQS TKGSTDITFW VGETGWPTDG 

       250        260        270        280        290        300 
TNFESSYPSV DNAKQFWKEG ICSMRAWGVN VIVFEAFDED WKPNTSGTSD VEKHWGVFTS 

       310 
SDNLKYSLDC DFS 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of a cell wall exo-beta-1,3-glucanase from Saccharomyces cerevisiae."
Klebl F., Tanner W.
J. Bacteriol. 171:6259-6264(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene."
Volckaert G., Voet M., Robben J.
Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"New potential cell wall glucanases of Saccharomyces cerevisiae and their involvement in mating."
Cappellaro C., Mrsa V., Tanner W.
J. Bacteriol. 180:5030-5037(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-34, SUBCELLULAR LOCATION.
Strain: ATCC 96099 / S288c / SEY6210.
[7]"Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
Norbeck J., Blomberg A.
FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-31.
Strain: ATCC 38531 / Y41.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31072 Genomic DNA. Translation: AAA34648.1.
Z73067 Genomic DNA. Translation: CAA97313.1.
AY558101 Genomic DNA. Translation: AAS56427.1.
BK006941 Genomic DNA. Translation: DAA08370.1.
PIRA33499.
RefSeqNP_011798.1. NM_001181411.1.

3D structure databases

ProteinModelPortalP15703.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33532. 68 interactions.
DIPDIP-6341N.
IntActP15703. 5 interactions.
MINTMINT-658203.
STRING4932.YGR282C.

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.
mycoCLAPEXG17B_YEAST.

2D gel databases

COMPLUYEAST-2DPAGEP15703.

Proteomic databases

PaxDbP15703.
PeptideAtlasP15703.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR282C; YGR282C; YGR282C.
GeneID853199.
KEGGsce:YGR282C.

Organism-specific databases

CYGDYGR282c.
SGDS000003514. BGL2.

Phylogenomic databases

eggNOGCOG5309.
GeneTreeENSGT00390000008245.
HOGENOMHOG000207150.
KOK01210.
OMASDCNTLQ.
OrthoDBEOG7WMCVT.

Enzyme and pathway databases

BioCycYEAST:YGR282C-MONOMER.

Gene expression databases

GenevestigatorP15703.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973368.

Entry information

Entry nameBGL2_YEAST
AccessionPrimary (citable) accession number: P15703
Secondary accession number(s): D6VV59
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries