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P15703

- BGL2_YEAST

UniProt

P15703 - BGL2_YEAST

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Protein

Glucan 1,3-beta-glucosidase

Gene

BGL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Glucanases possibly play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase.

Catalytic activityi

Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei233 – 2331NucleophileBy similarity
Active sitei292 – 2921Proton donorBy similarity

GO - Molecular functioni

  1. 1,3-beta-glucanosyltransferase activity Source: SGD
  2. chitin binding Source: UniProtKB-KW
  3. glucan endo-1,3-beta-D-glucosidase activity Source: SGD
  4. glucan exo-1,3-beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Chitin-binding

Enzyme and pathway databases

BioCyciYEAST:YGR282C-MONOMER.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.
mycoCLAPiEXG17B_YEAST.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan 1,3-beta-glucosidase (EC:3.2.1.58)
Alternative name(s):
Exo-1,3-beta-glucanase
GP29
Soluble cell wall protein 9
Gene namesi
Name:BGL2
Synonyms:SCW9
Ordered Locus Names:YGR282C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR282c.
SGDiS000003514. BGL2.

Subcellular locationi

Secretedcell wall 1 Publication
Note: Tightly bound to cell wall.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
  2. fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23232 PublicationsAdd
BLAST
Chaini24 – 313290Glucan 1,3-beta-glucosidasePRO_0000011896Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP15703.
PaxDbiP15703.
PeptideAtlasiP15703.

2D gel databases

COMPLUYEAST-2DPAGEP15703.

Expressioni

Gene expression databases

GenevestigatoriP15703.

Interactioni

Protein-protein interaction databases

BioGridi33532. 68 interactions.
DIPiDIP-6341N.
IntActiP15703. 5 interactions.
MINTiMINT-658203.
STRINGi4932.YGR282C.

Structurei

3D structure databases

ProteinModelPortaliP15703.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5309.
GeneTreeiENSGT00390000008245.
HOGENOMiHOG000207150.
InParanoidiP15703.
KOiK01210.
OMAiSDCNTLQ.
OrthoDBiEOG7WMCVT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15703 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRFSTTLATA ATALFFTASQ VSAIGELAFN LGVKNNDGTC KSTSDYETEL
60 70 80 90 100
QALKSYTSTV KVYAASDCNT LQNLGPAAEA EGFTIFVGVW PTDDSHYAAE
110 120 130 140 150
KAALQTYLPK IKESTVAGFL VGSEALYRND LTASQLSDKI NDVRSVVADI
160 170 180 190 200
SDSDGKSYSG KQVGTVDSWN VLVAGYNSAV IEASDFVMAN AFSYWQGQTM
210 220 230 240 250
QNASYSFFDD IMQALQVIQS TKGSTDITFW VGETGWPTDG TNFESSYPSV
260 270 280 290 300
DNAKQFWKEG ICSMRAWGVN VIVFEAFDED WKPNTSGTSD VEKHWGVFTS
310
SDNLKYSLDC DFS
Length:313
Mass (Da):34,119
Last modified:April 1, 1990 - v1
Checksum:i118D75528C5AD383
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31072 Genomic DNA. Translation: AAA34648.1.
Z73067 Genomic DNA. Translation: CAA97313.1.
AY558101 Genomic DNA. Translation: AAS56427.1.
BK006941 Genomic DNA. Translation: DAA08370.1.
PIRiA33499.
RefSeqiNP_011798.1. NM_001181411.1.

Genome annotation databases

EnsemblFungiiYGR282C; YGR282C; YGR282C.
GeneIDi853199.
KEGGisce:YGR282C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31072 Genomic DNA. Translation: AAA34648.1 .
Z73067 Genomic DNA. Translation: CAA97313.1 .
AY558101 Genomic DNA. Translation: AAS56427.1 .
BK006941 Genomic DNA. Translation: DAA08370.1 .
PIRi A33499.
RefSeqi NP_011798.1. NM_001181411.1.

3D structure databases

ProteinModelPortali P15703.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33532. 68 interactions.
DIPi DIP-6341N.
IntActi P15703. 5 interactions.
MINTi MINT-658203.
STRINGi 4932.YGR282C.

Protein family/group databases

CAZyi GH17. Glycoside Hydrolase Family 17.
mycoCLAPi EXG17B_YEAST.

2D gel databases

COMPLUYEAST-2DPAGE P15703.

Proteomic databases

MaxQBi P15703.
PaxDbi P15703.
PeptideAtlasi P15703.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR282C ; YGR282C ; YGR282C .
GeneIDi 853199.
KEGGi sce:YGR282C.

Organism-specific databases

CYGDi YGR282c.
SGDi S000003514. BGL2.

Phylogenomic databases

eggNOGi COG5309.
GeneTreei ENSGT00390000008245.
HOGENOMi HOG000207150.
InParanoidi P15703.
KOi K01210.
OMAi SDCNTLQ.
OrthoDBi EOG7WMCVT.

Enzyme and pathway databases

BioCyci YEAST:YGR282C-MONOMER.

Miscellaneous databases

NextBioi 973368.

Gene expression databases

Genevestigatori P15703.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a cell wall exo-beta-1,3-glucanase from Saccharomyces cerevisiae."
    Klebl F., Tanner W.
    J. Bacteriol. 171:6259-6264(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene."
    Volckaert G., Voet M., Robben J.
    Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "New potential cell wall glucanases of Saccharomyces cerevisiae and their involvement in mating."
    Cappellaro C., Mrsa V., Tanner W.
    J. Bacteriol. 180:5030-5037(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-34, SUBCELLULAR LOCATION.
    Strain: ATCC 96099 / S288c / SEY6210.
  7. "Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
    Norbeck J., Blomberg A.
    FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-31.
    Strain: ATCC 38531 / Y41.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBGL2_YEAST
AccessioniPrimary (citable) accession number: P15703
Secondary accession number(s): D6VV59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 29, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein strongly binds to glucan and chitin.
Present with 45000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3