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P15703

- BGL2_YEAST

UniProt

P15703 - BGL2_YEAST

Protein

Glucan 1,3-beta-glucosidase

Gene

BGL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Glucanases possibly play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase.

    Catalytic activityi

    Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei233 – 2331NucleophileBy similarity
    Active sitei292 – 2921Proton donorBy similarity

    GO - Molecular functioni

    1. 1,3-beta-glucanosyltransferase activity Source: SGD
    2. chitin binding Source: UniProtKB-KW
    3. glucan endo-1,3-beta-D-glucosidase activity Source: SGD
    4. glucan exo-1,3-beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. fungal-type cell wall organization Source: SGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Keywords - Ligandi

    Chitin-binding

    Enzyme and pathway databases

    BioCyciYEAST:YGR282C-MONOMER.

    Protein family/group databases

    CAZyiGH17. Glycoside Hydrolase Family 17.
    mycoCLAPiEXG17B_YEAST.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan 1,3-beta-glucosidase (EC:3.2.1.58)
    Alternative name(s):
    Exo-1,3-beta-glucanase
    GP29
    Soluble cell wall protein 9
    Gene namesi
    Name:BGL2
    Synonyms:SCW9
    Ordered Locus Names:YGR282C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR282c.
    SGDiS000003514. BGL2.

    Subcellular locationi

    Secretedcell wall 1 Publication
    Note: Tightly bound to cell wall.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-KW
    2. fungal-type cell wall Source: SGD

    Keywords - Cellular componenti

    Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23232 PublicationsAdd
    BLAST
    Chaini24 – 313290Glucan 1,3-beta-glucosidasePRO_0000011896Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP15703.
    PaxDbiP15703.
    PeptideAtlasiP15703.

    2D gel databases

    COMPLUYEAST-2DPAGEP15703.

    Expressioni

    Gene expression databases

    GenevestigatoriP15703.

    Interactioni

    Protein-protein interaction databases

    BioGridi33532. 68 interactions.
    DIPiDIP-6341N.
    IntActiP15703. 5 interactions.
    MINTiMINT-658203.
    STRINGi4932.YGR282C.

    Structurei

    3D structure databases

    ProteinModelPortaliP15703.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5309.
    GeneTreeiENSGT00390000008245.
    HOGENOMiHOG000207150.
    KOiK01210.
    OMAiSDCNTLQ.
    OrthoDBiEOG7WMCVT.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15703-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRFSTTLATA ATALFFTASQ VSAIGELAFN LGVKNNDGTC KSTSDYETEL    50
    QALKSYTSTV KVYAASDCNT LQNLGPAAEA EGFTIFVGVW PTDDSHYAAE 100
    KAALQTYLPK IKESTVAGFL VGSEALYRND LTASQLSDKI NDVRSVVADI 150
    SDSDGKSYSG KQVGTVDSWN VLVAGYNSAV IEASDFVMAN AFSYWQGQTM 200
    QNASYSFFDD IMQALQVIQS TKGSTDITFW VGETGWPTDG TNFESSYPSV 250
    DNAKQFWKEG ICSMRAWGVN VIVFEAFDED WKPNTSGTSD VEKHWGVFTS 300
    SDNLKYSLDC DFS 313
    Length:313
    Mass (Da):34,119
    Last modified:April 1, 1990 - v1
    Checksum:i118D75528C5AD383
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31072 Genomic DNA. Translation: AAA34648.1.
    Z73067 Genomic DNA. Translation: CAA97313.1.
    AY558101 Genomic DNA. Translation: AAS56427.1.
    BK006941 Genomic DNA. Translation: DAA08370.1.
    PIRiA33499.
    RefSeqiNP_011798.1. NM_001181411.1.

    Genome annotation databases

    EnsemblFungiiYGR282C; YGR282C; YGR282C.
    GeneIDi853199.
    KEGGisce:YGR282C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31072 Genomic DNA. Translation: AAA34648.1 .
    Z73067 Genomic DNA. Translation: CAA97313.1 .
    AY558101 Genomic DNA. Translation: AAS56427.1 .
    BK006941 Genomic DNA. Translation: DAA08370.1 .
    PIRi A33499.
    RefSeqi NP_011798.1. NM_001181411.1.

    3D structure databases

    ProteinModelPortali P15703.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33532. 68 interactions.
    DIPi DIP-6341N.
    IntActi P15703. 5 interactions.
    MINTi MINT-658203.
    STRINGi 4932.YGR282C.

    Protein family/group databases

    CAZyi GH17. Glycoside Hydrolase Family 17.
    mycoCLAPi EXG17B_YEAST.

    2D gel databases

    COMPLUYEAST-2DPAGE P15703.

    Proteomic databases

    MaxQBi P15703.
    PaxDbi P15703.
    PeptideAtlasi P15703.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR282C ; YGR282C ; YGR282C .
    GeneIDi 853199.
    KEGGi sce:YGR282C.

    Organism-specific databases

    CYGDi YGR282c.
    SGDi S000003514. BGL2.

    Phylogenomic databases

    eggNOGi COG5309.
    GeneTreei ENSGT00390000008245.
    HOGENOMi HOG000207150.
    KOi K01210.
    OMAi SDCNTLQ.
    OrthoDBi EOG7WMCVT.

    Enzyme and pathway databases

    BioCyci YEAST:YGR282C-MONOMER.

    Miscellaneous databases

    NextBioi 973368.

    Gene expression databases

    Genevestigatori P15703.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a cell wall exo-beta-1,3-glucanase from Saccharomyces cerevisiae."
      Klebl F., Tanner W.
      J. Bacteriol. 171:6259-6264(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. "Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene."
      Volckaert G., Voet M., Robben J.
      Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "New potential cell wall glucanases of Saccharomyces cerevisiae and their involvement in mating."
      Cappellaro C., Mrsa V., Tanner W.
      J. Bacteriol. 180:5030-5037(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-34, SUBCELLULAR LOCATION.
      Strain: ATCC 96099 / S288c / SEY6210.
    7. "Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
      Norbeck J., Blomberg A.
      FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-31.
      Strain: ATCC 38531 / Y41.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBGL2_YEAST
    AccessioniPrimary (citable) accession number: P15703
    Secondary accession number(s): D6VV59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein strongly binds to glucan and chitin.
    Present with 45000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3